RESUMEN
The present research was an examination of the concentration levels of 10 metal(loid)s that had accumulated in the muscle, gills, and liver tissues of Carassius gibelio (Prussian carp) fish. The fish species, used as samples in the study, were obtained from the part of the river in Gülsehir (Türkiye). Besides, possible health risks to consumers were specified based on the daily estimated intake (EDI) of metal(loid)s in adults. Additionally, considering the inhabitants' frequency of fish consumption in the area, target hazard quotient (THQ), hazard index (HI), carcinogenic risk (CR), and maximum safe consumption quantity (MSCQ) values were assessed. All of the examined metal(loid)s in the research were determined in all of the tissues of the fish. Also, Fe and Zn had the highest levels in the tissues, and Pb had the lowest level. There were statistically significant differences of Fe, Co, Cu, Zn, As, Cd, and Pb among the tissues (p < 0.05). It was determined that the gender of the fish did not affect the accumulation of the metal(loid)s in the tissues. According to the research results, no significant statistical difference was found between the accumulation of the metal(loid)s (except Ni) and the size of the fish (length and weight) (p > 0.05). The estimated daily intake levels of the metal(loid)s accumulating in the eatable muscle of the samples were calculated to be lower than the reference dose limit. Besides, the THQ and the HI values lower than 1 were found. Carcinogenic risk values related to inorganic As were below 10-6. This result specifies that consumption of the fish poses no carcinogenic risks to consumers' health. It was concluded that less than 70 g of C. gibelio daily consumption would not harm consumers' health.
RESUMEN
Hexavalent chromium Cr (VI) causes various toxic and carcinogenic effects. The main carcinogenic effect is observed in the pulmonary system through inhalation route. Reduction of Cr (VI) to Cr (V, IV, and III) reactive intermediates within the cells by intracellular reducing agents such as glutathione is an important event leading to oxidative stress and oxidative DNA damage. This study evaluated the effects of intraperitoneal administration of Cr (VI) and GSH on total oxidant status (TOS), total antioxidant capacity (TAC), oxidative stress index, and oxidative DNA damage by evaluating the level of 8-hydroxy-2Ì-deoxyguanosine (8-OHdG) in Swiss-Albino mice. Seventy two mice were divided into 6 groups and treated intraperitoneally as follow: control (saline), group GSH (30 mg/kg GSH) groups of Cr-20 (20 mg/kg, K2Cr2O7), Cr-30 (30 mg/kg K2Cr2O7), Cr-20 + GSH (20 mg/kg K2Cr2O7 + 30 mg/kg GSH), Cr-30 + GSH (30 mg/kg K2Cr2O7 + 30 mg/kg GSH). Total oxidant capacities of Cr-20 and Cr-30 were increased compared to control, Cr-20 + GSH, and Cr-30 + GSH. TOS levels in Cr-20 + GSH and Cr-30 + GSH were lower than in Cr-20 and Cr-30. No difference in TAC was observed among the groups. 8-Hydroxy-2Ì-deoxyguanosine levels were increased in groups Cr-20 and Cr-30 compared with control and groups Cr-20 + GSH and Cr-30 + GSH. No difference was determined in 8-OHdG levels among control, groups GSH, Cr-20 + GSH and Cr-30 + GSH. Results indicate that Cr (VI) given i.p. route causes increased oxidative stress and oxidative DNA damage in the blood of Swiss-Albino mice. Administration of GSH via i.p. route protects from oxidative stress and DNA damage.