RESUMEN
Experimental autoimmune encephalomyelitis (EAE) is a mouse model of demyelinating diseases, such as multiple sclerosis (MS). MS can be accompanied by autoimmune hepatitis. In this study, nanomechanical, biorheological and histological examinations were carried out by atomic force microscopy (AFM), rheology, and immunofluorescence microscopy to investigate changes in the liver tissue of EAE mice and the effect of natalizumab, a monoclonal antibody against α4-integrin (VLA-4) cell adhesion molecule, used in MS therapy. Liver samples collected from EAE mice in three successive phases of the disease showed inflammatory changes manifested by leukocyte infiltrations and elevated levels of proinflammatory cytokine IL-1ß. Liver stiffness and viscoelasticity increased in the onset phase of EAE, decreased in the peak phase and increased again in the chronic phase to reach the highest values. These changes were not associated with inflammation parameters which increased in the peak phase and decreased to the lowest values in the chronic phase. Moreover, anti-VLA treatment, which reduced the inflammation parameters, had an ambiguous effect on stiffness and viscoelasticity: it increased them in the peak phase but decreased in the chronic phase. The observed discrepancies can result from a complex network of interactions between inflammation and fibrosis, as well as between liver cells and the extracellular matrix influencing the biomechanical properties of the liver tissue.
Asunto(s)
Encefalomielitis Autoinmune Experimental , Esclerosis Múltiple , Animales , Anticuerpos Monoclonales , Modelos Animales de Enfermedad , Inflamación , Integrina alfa4beta1 , Hígado , Ratones , Ratones Endogámicos C57BLRESUMEN
A traditional photonic-force microscope (PFM) results in huge sets of data, which requires tedious numerical analysis. In this paper, we propose instead an analog signal processor to attain real-time capabilities while retaining the richness of the traditional PFM data. Our system is devoted to intracellular measurements and is fully interactive through the use of a haptic joystick. Using our specialized analog hardware along with a dedicated algorithm, we can extract the full 3D stiffness matrix of the optical trap in real time, including the off-diagonal cross-terms. Our system is also capable of simultaneously recording data for subsequent offline analysis. This allows us to check that a good correlation exists between the classical analysis of stiffness and our real-time measurements. We monitor the PFM beads using an optical microscope. The force-feedback mechanism of the haptic joystick helps us in interactively guiding the bead inside living cells and collecting information from its (possibly anisotropic) environment. The instantaneous stiffness measurements are also displayed in real time on a graphical user interface. The whole system has been built and is operational; here we present early results that confirm the consistency of the real-time measurements with offline computations.
Asunto(s)
Microscopía/instrumentación , Microscopía/métodos , Rayos LáserRESUMEN
Among all the fossils, petrified wood belongs to the most impressive and most common of materials. Still, its study has not exceeded the purely phenomenological level. The recognition of the conserved structure of petrified wood seems to be of meaning for understanding the geological past, the complete carbon cycle inside the Earth, and the structure of potential new materials. The first ever published spatial distributions of the remains of the primordial organic material (lignin, cellulose, pectins) in the cells of permineralized wood, from Dunarobba (Central Italy), are presented here. They were collected using micro-Raman spectrometry. The composite nature of the petrified material (calcite located in the lumena of cells and goethite located in the cell walls) was confirmed by electron, proton, and X-ray microprobes. The structure of the cell walls was well preserved. The mineralization process was induced by the tracheidal water flow and was stopped after formation of pipe-like goethite shielding of the cell walls on the cellulose scaffolds. The chemical (Eh and pH ranges) and probable microbial conditions for such a pattern of mineralization were determined. We estimate that substantial amounts of the primordial organic matter were preserved in bodies of petrified wood on a global scale. The wood petrifaction process, if well understood, can be a basis for the production of "everlasting" organic-inorganic composite compounds.
Asunto(s)
Fósiles , Espectrometría Raman/métodos , Madera/química , Microanálisis por Sonda Electrónica , Protones , Espectrometría por Rayos X , Madera/ultraestructuraRESUMEN
In recent years, a single ion hit facility has been constructed at the IFJ ion microprobe. The setup is used for the precise irradiations of living cells by a controlled number of ions. Investigations of such type have two very important requirements: (1) cells must be examined in their natural state and environment (i.e. without previously being killed, preferentially neither fixed nor stained) and (2) the possibility of automatic irradiation of large number of cells (including computer recognition of cells positions) must be provided. This work presents some of the crucial features of the off-line and on-line optical systems, including self-developed software responsible for automatic cell recognition.
Asunto(s)
Técnicas de Cultivo de Célula/instrumentación , Separación Celular/instrumentación , Iones Pesados , Interpretación de Imagen Asistida por Computador/métodos , Microscopía/instrumentación , Aceleradores de Partículas/instrumentación , Radiometría/instrumentación , Técnicas de Cultivo de Célula/métodos , Separación Celular/métodos , Diseño de Equipo , Análisis de Falla de Equipo , Alemania , Interpretación de Imagen Asistida por Computador/instrumentación , Iones , Microscopía/métodos , Miniaturización , Sistemas en Línea , Dosis de Radiación , Radiometría/métodos , Sensibilidad y Especificidad , Evaluación de la Tecnología BiomédicaRESUMEN
The cell's cytoskeleton together with the cell membrane and numerous accessory proteins determines the mechanical properties of cell. Any factors influencing cell organization and structure can cause alterations in mechanical properties of cell (its ability for deformation and adhesion). The determination of the local elastic properties of cells in their culture conditions has opened the possibility for the measurement of the influence of different factors on the mechanical properties of the living cells. The effect of the chitosan on the stiffness of the non-malignant transitional epithelial cells of ureter (HCV 29) and the transitional cell cancer of urine bladder (T24) was determined using scanning force microscopy. The investigations were performed in the culture medium (RPMI 1640) containing 10% fetal calf serum in the presence of the microcrystalline chitosan of the three different deacetylation degrees. In parallel, the effect of chitosan on production of lactate and ATP level was determined. The results showed the strong correlation between the decrease of the energy production and the increase in Young's modulus values obtained for the cancer cells treated with chitosan.
Asunto(s)
Quitina/farmacología , Vejiga Urinaria/efectos de los fármacos , Membrana Celular/efectos de los fármacos , Células Cultivadas , Quitina/análogos & derivados , Quitosano , Citoesqueleto/efectos de los fármacos , Glucólisis/efectos de los fármacos , Humanos , Microscopía de Fuerza Atómica , Células Tumorales CultivadasRESUMEN
Synchrotron radiation-Fourier transform infrared (SR-FTIR) microscopy coupled with multivariate data analysis was used as an independent modality to monitor the cellular bystander effect. Single, living prostate cancer PC-3 cells were irradiated with various numbers of protons, ranging from 50-2,000, with an energy of either 1 or 2 MeV using a proton microprobe. SR-FTIR spectra of cells, fixed after exposure to protons and nonirradiated neighboring cells (bystander cells), were recorded. Spectral differences were observed in both the directly targeted and bystander cells and included changes in the DNA backbone and nucleic bases, along with changes in the protein secondary structure. Principal component analysis (PCA) was used to investigate the variance in the entire data set. The percentage of bystander cells relative to the applied number of protons with two different energies was calculated. Of all the applied quantities, the dose of 400 protons at 2 MeV was found to be the most effective for causing significant macromolecular perturbation in bystander PC-3 cells.
Asunto(s)
Efecto Espectador/efectos de la radiación , Análisis de Componente Principal , Espectroscopía Infrarroja por Transformada de Fourier , Línea Celular Tumoral , ADN/química , Reparación del ADN , Humanos , Masculino , Conformación de Ácido NucleicoRESUMEN
The SFM setup has been used to image the three-dimensional surface structures of various biological specimens in air and in liquid cell. The samples like red blood cell (RBC), lymphocytes, aggregates of pronase, and surface of bone measured in air, and macrophage surface measured in PBS solution are presented.
Asunto(s)
Huesos/ultraestructura , Células/ultraestructura , Microscopía de Fuerza Atómica , Pronasa/ultraestructura , Manejo de Especímenes/métodos , Humanos , Propiedades de SuperficieRESUMEN
We propose a system to characterize the 3-D diffusion properties of the probing bead trapped by a photonic-force microscope. We follow a model-based approach, where the model of the dynamics of the bead is given by the Langevin equation. Our procedure combines software and analog hardware to measure the corresponding stiffness matrix. We are able to estimate all its elements in real time, including off-diagonal terms. To achieve our goal, we have built a simple analog computer that performs a continuous preprocessing of the data, which can be subsequently digitized at a much lower rate than is otherwise required. We also provide an effective numerical algorithm for compensating the correlation bias introduced by a quadrant photodiode detector in the microscope. We validate our approach using simulated data and show that our bias-compensation scheme effectively improves the accuracy of the system. Moreover, we perform experiments with the real system and demonstrate real-time capabilities. Finally, we suggest a simple adjunction that would allow one to determine the mass matrix as well.
Asunto(s)
Algoritmos , Microscopía , Programas Informáticos , Simulación por Computador , Elasticidad , Microscopía/instrumentación , Microscopía/métodos , Método de Montecarlo , Pinzas Ópticas , Reproducibilidad de los ResultadosRESUMEN
Scanning force microscopy was used for the determination of the elastic properties of living cells in their culture conditions. The studies were carried out on human epithelial cells. Two similar lines of normal cells (Hu609 and HCV29) and three cancerous ones (Hu456, T24, BC3726) were measured using the scanning force microscope in order to collect the force versus indentation curves. The BC3726 line originates from the HCV29 cell line which was transformed by the v-ras oncogene. To evaluate their elastic properties, Young's modulus values were determined. The present study has shown that normal cells have a Young's modulus of about one order of magnitude higher than cancerous ones. Such a change might be attributed to a difference in the organisation of cell cytoskeletons and requires further studies.