Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 24
Filtrar
1.
Fungal Genet Biol ; 82: 158-67, 2015 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-26212074

RESUMEN

In the filamentous fungus Neurospora crassa, phospholipase C may play a role in hyphal extension at the growing tips as part of a growth-sensing mechanism that activates calcium release from internal stores to mediate continued expansion of the hyphal tip. One candidate for a tip-localized phospholipase C is PLC-1. We characterized morphology and growth characteristics of a knockout mutant (KO plc-1) and a RIP mutated strain (RIP plc-1) (missense mutations and a nonsense mutation render the gene product non-functional). Growth and hyphal cytology of wildtype and KO plc-1 were similar, but the RIP plc-1 mutant grew slower and exhibited abnormal membrane structures at the hyphal tip, imaged using the fluorescence dye FM4-64. To test for causes of the slower growth of the RIP plc-1 mutant, we examined its physiological poise compared to wildtype and the KO plc-1 mutant. The electrical properties of all three strains and the electrogenic contribution of the plasma membrane H(+)-ATPase (identified by cyanide inhibition) were the same. Responses to high osmolarity were also similar. However, the RIP plc-1 mutant had a significantly lower turgor, a possible cause of its slower growth. While growth of all three strains was inhibited by the phospholipase C inhibitor 3-nitrocoumarin, the RIP plc-1 mutant did not exhibit hyphal bursting after addition of the inhibitor, observed in both wildtype and the KO plc-1 mutant. Although the plc-1 gene is not obligatory for tip growth, the phenotype of the RIP plc-1 mutant - abnormal tip cytology, lower turgor and resistance to inhibitor-induced hyphal bursting - suggest it does play a role in tip growth. The expression of a dysfunctional plc-1 gene may cause a shift to alternative mechanism(s) of growth sensing in hyphal extension.


Asunto(s)
Estudios de Asociación Genética , Mutación , Neurospora crassa/genética , Neurospora crassa/metabolismo , Fenotipo , Fosfolipasas de Tipo C/genética , Calcio/metabolismo , Fenómenos Electrofisiológicos , Inhibidores Enzimáticos/farmacología , Técnicas de Inactivación de Genes , Genotipo , Hifa , Neurospora crassa/citología , Neurospora crassa/efectos de los fármacos , Fosfolipasas de Tipo C/antagonistas & inhibidores , Fosfolipasas de Tipo C/metabolismo
2.
Microbiology (Reading) ; 159(Pt 11): 2386-2394, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-23970568

RESUMEN

Movement of nuclei, mitochondria and vacuoles through hyphal trunks of Neurospora crassa were vector-mapped using fluorescent markers and green fluorescent protein tags. The vectorial movements of all three were strongly correlated, indicating the central role of mass (bulk) flow in cytoplasm movements in N. crassa. Profiles of velocity versus distance from the hyphal wall did not match the parabolic shape predicted by the ideal Hagen-Poiseuille model of flow at low Reynolds number. Instead, the profiles were flat, consistent with a model of partial plug flow due to the high concentration of organelles in the flowing cytosol. The intra-hyphal pressure gradients were manipulated by localized external osmotic treatments to demonstrate the dependence of velocity (and direction) on pressure gradients within the hyphae. The data support the concept that mass transport, driven by pressure gradients, dominates intra-hyphal transport. The transport occurs by partial plug flow due to the organelles in the cytosol.


Asunto(s)
Transporte Biológico , Hifa/fisiología , Neurospora crassa/fisiología , Orgánulos/fisiología , Genes Reporteros , Proteínas Fluorescentes Verdes/análisis , Proteínas Fluorescentes Verdes/genética , Hifa/metabolismo , Microscopía Fluorescente , Neurospora crassa/metabolismo , Orgánulos/metabolismo , Presión Osmótica , Coloración y Etiquetado/métodos
3.
New Phytol ; 220(1): 8-9, 2018 10.
Artículo en Inglés | MEDLINE | ID: mdl-30156021
4.
Eukaryot Cell ; 11(5): 694-702, 2012 May.
Artículo en Inglés | MEDLINE | ID: mdl-22408225

RESUMEN

We characterized the electrical phenotypes of mutants with mutations in genes encoding calcium transporters-a mechanosensitive channel homolog (MscS), a Ca(2+)/H(+) exchange protein (cax), and Ca(2+)-ATPases (nca-1, nca-2, nca-3)-as well as those of double mutants (the nca-2 cax, nca-2 nca-3, and nca-3 cax mutants). The electrical characterization used dual impalements to obtain cable-corrected current-voltage measurements. Only two types of mutants (the MscS mutant; the nca-2 mutant and nca-2-containing double mutants) exhibited lower resting potentials. For the nca-2 mutant, on the basis of unchanged conductance and cyanide-induced depolarization of the potential, the cause is attenuated H(+)-ATPase activity. The growth of the nca-2 mutant-containing strains was inhibited by elevated extracellular Ca(2+) levels, indicative of lesions in Ca(2+) homeostasis. However, the net Ca(2+) effluxes of the nca-2 mutant, measured noninvasively with a self-referencing Ca(2+)-selective microelectrode, were similar to those of the wild type. All of the mutants exhibited osmosensitivity similar to that of the wild type (the turgor of the nca-2 mutant was also similar to that of the wild type), suggesting that Ca(2+) signaling does not play a role in osmoregulation. The hyphal tip morphology and tip-localized mitochondria of the nca-2 mutant were similar to those of the wild type, even when the external [Ca(2+)] was elevated. Thus, although Ca(2+) homeostasis is perturbed in the nca-2 mutant (B. J. Bowman et al., Eukaryot. Cell 10:654-661, 2011), the phenotype does not extend to tip growth or to osmoregulation but is revealed by lower H(+)-ATPase activity.


Asunto(s)
Señalización del Calcio , Calcio/metabolismo , Genes Fúngicos , Neurospora crassa/fisiología , Membrana Celular/metabolismo , Medios de Cultivo/metabolismo , Técnicas de Cultivo , Cianuros/farmacología , Citoplasma/metabolismo , Proteínas Fúngicas/metabolismo , Homeostasis , Hifa/crecimiento & desarrollo , Hifa/fisiología , Potenciales de la Membrana , Microelectrodos , Mitocondrias/fisiología , Neurospora crassa/crecimiento & desarrollo , Neurospora crassa/metabolismo , Fenotipo , ATPasas de Translocación de Protón Vacuolares/metabolismo
5.
Eukaryot Cell ; 10(6): 832-41, 2011 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-21357477

RESUMEN

The role of Mid1, a stretch-activated ion channel capable of being permeated by calcium, in ascospore development and forcible discharge from asci was examined in the pathogenic fungus Gibberella zeae (anamorph Fusarium graminearum). The Δmid1 mutants exhibited a >12-fold reduction in ascospore discharge activity and produced predominately abnormal two-celled ascospores with constricted and fragile septae. The vegetative growth rate of the mutants was ∼50% of the wild-type rate, and production of macroconidia was >10-fold lower than in the wild type. To better understand the role of calcium flux, Δmid1 Δcch1 double mutants were also examined, as Cch1, an L-type calcium ion channel, is associated with Mid1 in Saccharomyces cerevisiae. The phenotype of the Δmid1 Δcch1 double mutants was similar to but more severe than the phenotype of the Δmid1 mutants for all categories. Potential and current-voltage measurements were taken in the vegetative hyphae of the Δmid1 and Δcch1 mutants and the wild type, and the measurements for all three strains were remarkably similar, indicating that neither protein contributes significantly to the overall electrical properties of the plasma membrane. Pathogenicity of the Δmid1 and Δmid1Δcch1 mutants on the host (wheat) was not affected by the mutations. Exogenous calcium supplementation partially restored the ascospore discharge and vegetative growth defects for all mutants, but abnormal ascospores were still produced. These results extend the known roles of Mid1 to ascospore development and forcible discharge. However, Neurospora crassa Δmid1 mutants were also examined and did not exhibit defects in ascospore development or in ascospore discharge. In comparison to ion channels in other ascomycetes, Mid1 shows remarkable adaptability of roles, particularly with regard to niche-specific adaptation.


Asunto(s)
Canales de Calcio/metabolismo , Proteínas Fúngicas/metabolismo , Gibberella/crecimiento & desarrollo , Mecanotransducción Celular , Esporas Fúngicas/metabolismo , Señalización del Calcio/genética , Proliferación Celular , Medios de Cultivo , Técnicas de Inactivación de Genes , Gibberella/fisiología , Potenciales de la Membrana
6.
Plant Cell Physiol ; 51(11): 1889-99, 2010 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-20926416

RESUMEN

Plasma membrane fluxes of the large unicellular model algal cell Eremosphaera viridis (De Bary) were measured under various light regimes to explore the role of plasma membrane fluxes during photosynthesis and high light-induced chloroplast translocation. Plasma membrane fluxes were measured directly and non-invasively with self-referencing ion-selective (H(+), Ca(2+), K(+) and Cl(-)) potentiometric microelectrodes and oxygen amperometric microelectrodes. At light irradiances high enough to induce chloroplast migration from the cell periphery to its center, oxygen evolution declined to respiratory net O(2) uptake prior to any significant chloroplast translocation, while net K(+) and Cl(-) influx increased during the decline in photosynthetic activity (and the membrane potential depolarized). The results suggest that chloroplast translocation is not the cause of the cessation of O(2) evolution at high irradiance. Rather, the chloroplast translocation may play a protective role: shielding the centrally located nucleus from damaging light intensities. At both high and low light intensities (similar to ambient growth conditions), there was a strong inverse correlation between H(+) net fluxes and respiratory and photosynthetic net O(2) fluxes. A similar inverse relationship was also observed for Ca(2+) net fluxes, but only at higher light intensities. The net H(+) fluxes are small relative to the buffering capacity of the cell, but are clearly related to both photosynthetic and respiratory activity.


Asunto(s)
Chlorophyta/metabolismo , Iones/metabolismo , Oxígeno/metabolismo , Transporte Biológico , Chlorophyta/fisiología , Cloroplastos/metabolismo , Potenciales de la Membrana , Microelectrodos , Fotosíntesis
7.
Fungal Genet Biol ; 47(8): 721-6, 2010 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-20546911

RESUMEN

The internal hydrostatic pressure (turgor) of the filamentous fungus Neurospora crassa is regulated at about 400-500 kiloPascals, primarily by an osmotic MAP kinase cascade which activates ion uptake from the extracellular medium and glycerol synthesis. In the absence of hyperosmotic stress, the phenylpyrrole fungicide fludioxonil activates the osmotic MAP kinase cascade, resulting in cell death. Turgor, the electrical potential and net ion fluxes were measured after treatment with fludioxonil. In wildtype, fludioxonil causes a hyperpolarization of the plasma membrane and net H(+) efflux from the cell, consistent with activation of the H(+)-ATPase. At the same time, net K(+) uptake occurs, and turgor increases (about 2-fold above normal levels). None of these changes are observed in the os-2 mutant (which lacks a functional MAP kinase, the last of the three kinases in the osmotic MAP kinase cascade). Tip growth ceases as hyperpolarization, net ion flux changes, and turgor increases begin. The inappropriate turgor increase is the probable cause of eventual lysis and death. The results corroborate a multi-pathway response to hyperosmotic stress that includes activation of plasma membrane transport. The relation to cell expansion (tip growth) is not direct. Increases in turgor due to ion transport might be expected to increase growth rate, but this does not occur. Instead, there must be a complex regulatory interplay between the growth and the turgor driving force, possibly mediated by regulation of cell wall extensibility.


Asunto(s)
Dioxoles/toxicidad , Iones/metabolismo , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Neurospora crassa/efectos de los fármacos , Presión Osmótica , Pirroles/toxicidad , Antifúngicos/toxicidad , Membrana Celular/efectos de los fármacos , Proteínas Fúngicas/metabolismo , Hidrógeno/metabolismo , Potenciales de la Membrana/efectos de los fármacos , Neurospora crassa/crecimiento & desarrollo , Potasio/metabolismo
8.
Fungal Genet Biol ; 46(12): 949-55, 2009 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-19772928

RESUMEN

Hyphal tip-growing organisms often rely upon an internal hydrostatic pressure (turgor) to drive localized expansion of the cell. Regulation of the turgor in response to osmotic shock is mediated primarily by an osmotic MAP kinase cascade which activates osmolyte synthesis and ion uptake to effect turgor recovery. We characterized a Neurospora crassa homolog (PTK2) of ser/thr kinase regulators of ion transport in yeast to determine its role in turgor regulation in a filamentous fungi. The ptk2 mutant is osmosensitive, and has lower turgor poise than wildtype. The cause appears to be lower activity of the plasma membrane H+-ATPase. Its role in osmoadaptation is unrelated to the activity of the osmotic MAP kinase cascade. Instead, it acts in an alternative pathway that, like the osmotic MAP kinase cascade, also involves ion transport mediated osmoadaptation.


Asunto(s)
Adaptación Fisiológica , Proteínas Fúngicas/metabolismo , Neurospora crassa/fisiología , Proteínas Serina-Treonina Quinasas/metabolismo , Permeabilidad de la Membrana Celular , Proteínas Fúngicas/genética , Homeostasis , Presión Hidrostática , Hifa/crecimiento & desarrollo , Transporte Iónico , Sistema de Señalización de MAP Quinasas , Potenciales de la Membrana , Mutación , Neurospora crassa/enzimología , Neurospora crassa/genética , Concentración Osmolar , Presión Osmótica , Técnicas de Placa-Clamp , Proteínas Serina-Treonina Quinasas/genética , ATPasas de Translocación de Protón/genética , ATPasas de Translocación de Protón/metabolismo , Saccharomyces cerevisiae/fisiología
9.
Eukaryot Cell ; 7(4): 647-55, 2008 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-18296620

RESUMEN

In the yeast Saccharomyces cerevisiae, the MID1 (mating-induced death) gene encodes a stretch-activated channel which is required for successful mating; the mutant phenotype is rescued by elevated extracellular calcium. Homologs of the MID1 gene are found in fungi that are morphologically complex compared to yeast, both Basidiomycetes and Ascomycetes. We explored the phenotype of a mid-1 knockout mutant in the filamentous ascomycete Neurospora crassa. The mutant exhibits lower growth vigor than the wild type (which is not rescued by replete calcium) and mates successfully. Thus, the role of the MID-1 protein differs from that of the homologous gene product in yeast. Hyphal cytology, growth on diverse carbon sources, turgor regulation, and circadian rhythms of the mid-1 mutant are all similar to those of the wild type. However, basal turgor is lower than wild type, as is the activity of the plasma membrane H(+)-ATPase (measured by cyanide [CN(-)]-induced depolarization of the energy-dependent component of the membrane potential). In addition, the mutant is unable to grow at low extracellular Ca(2+) levels or when cytoplasmic Ca(2+) is elevated with the Ca(2+) ionophore A23187. We conclude that the MID-1 protein plays a role in regulation of ion transport via Ca(2+) homeostasis and signaling. In the absence of normal ion transport activity, the mutant exhibits poorer growth.


Asunto(s)
Canales de Calcio/genética , Proteínas Fúngicas/genética , Neurospora crassa/fisiología , Adenosina Trifosfatasas/metabolismo , Canales de Calcio/metabolismo , Ritmo Circadiano , Proteínas Fúngicas/metabolismo , Mecanorreceptores/metabolismo , Mutación
10.
FEMS Microbiol Lett ; 233(1): 125-30, 2004 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-15043878

RESUMEN

Oxygen fluxes were mapped at the growing apices and along mycelial hyphal segments of the ascomycete Neurospora crassa. High spatial resolution was obtained using micro-oxygen probes (2-3 microm tip diameters) and the self-referencing technique to maximize the sensitivity of oxygen flux measurements. As expected, oxygen influx was inhibited by cyanide, although oxygen influx (and hyphal growth) resumed with the induction of an alternate oxidase activity. Along hyphal segments, variations in oxygen influx were not correlated with location, near or far from septa, and varied over time along the same hyphal segment. Growing hyphae had a region of maximal oxygen influx greater than 10 microm behind the hyphal tip, the oxygen influx was correlated with hyphal growth rate. The region of maximal oxygen influx did not correspond with mitochondrial density, which is maximal (about 30% of hyphal volume) 5-10 microm behind the tip. Therefore, tip-localized mitochondria do not contribute to the respiratory requirements of the growing hypha. The tip-localized mitochondria may function in clearing calcium from the cytoplasm, although a decline in chlortetracycline fluorescence after cyanide inhibition could also be due to ATP-depletion due to inhibition of actively respiring mitochondria. Alternatively, the growing tip may be the site of mitochondrial biogenesis.


Asunto(s)
Hifa/crecimiento & desarrollo , Hifa/metabolismo , Neurospora crassa/crecimiento & desarrollo , Neurospora crassa/metabolismo , Oxígeno/metabolismo , Calcio/metabolismo , Cianuros/toxicidad , Mitocondrias/metabolismo , Oxidorreductasas/metabolismo
11.
Nat Rev Microbiol ; 9(7): 509-18, 2011 Jun 06.
Artículo en Inglés | MEDLINE | ID: mdl-21643041

RESUMEN

The mechanisms underlying the growth of fungal hyphae are rooted in the physical property of cell pressure. Internal hydrostatic pressure (turgor) is one of the major forces driving the localized expansion at the hyphal tip which causes the characteristic filamentous shape of the hypha. Calcium gradients regulate tip growth, and secretory vesicles that contribute to this process are actively transported to the growing tip by molecular motors that move along cytoskeletal structures. Turgor is controlled by an osmotic mitogen-activated protein kinase cascade that causes de novo synthesis of osmolytes and uptake of ions from the external medium. However, as discussed in this Review, turgor and pressure have additional roles in hyphal growth, such as causing the mass flow of cytoplasm from the basal mycelial network towards the expanding hyphal tips at the colony edge.


Asunto(s)
Hongos/crecimiento & desarrollo , Hongos/fisiología , Hifa/crecimiento & desarrollo , Fenómenos Biofísicos , Citoplasma/fisiología , Hongos/metabolismo , Hifa/metabolismo , Neurospora crassa/crecimiento & desarrollo , Neurospora crassa/metabolismo , Neurospora crassa/fisiología , Presión Osmótica
12.
Fungal Biol ; 115(6): 446-74, 2011 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-21640311

RESUMEN

Neurospora crassa has been at the forefront of biological research from the early days of biochemical genetics to current progress being made in understanding gene and genetic network function. Here, we discuss recent developments in analysis of the fundamental form of fungal growth, development and proliferation -- the hypha. Understanding the establishment and maintenance of polarity, hyphal elongation, septation, branching and differentiation are at the core of current research. The advances in the identification and functional dissection of regulatory as well as structural components of the hypha provide an expanding basis for elucidation of fundamental attributes of the fungal cell. The availability and continuous development of various molecular and microscopic tools, as utilized by an active and co-supportive research community, promises to yield additional important new discoveries on the biology of fungi.


Asunto(s)
Polaridad Celular , Hifa/citología , Neurospora crassa/crecimiento & desarrollo , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Hifa/genética , Hifa/crecimiento & desarrollo , Hifa/metabolismo , Modelos Biológicos , Neurospora crassa/citología , Neurospora crassa/genética , Neurospora crassa/metabolismo
13.
Microbiology (Reading) ; 155(Pt 3): 903-911, 2009 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-19246761

RESUMEN

Fungal cells maintain an internal hydrostatic pressure (turgor) of about 400-500 kPa. In the filamentous fungus Neurospora crassa, the initial cellular responses to hyperosmotic treatment are loss of turgor, a decrease in relative hyphal volume per unit length (within 1 min) and cell growth arrest; all recover over a period of 10-60 min due to increased net ion uptake and glycerol production. The electrical responses to hyperosmotic treatment are a transient depolarization of the potential (within 1 min), followed by a sustained hyperpolarization (after 4 min) to a potential more negative than the initial potential (a driving force for ion uptake). The nature of the transient depolarization was explored in the context of other transient responses to hyperosmotic shock, to determine whether activation of a specific ion permeability or some other rapid change in electrogenic transport was responsible. Changing the ionic composition of the extracellular medium revealed that K(+) permeability increases and H(+) permeability declines during the transient depolarization. We suggest that these changes are due to concerted inhibition of the electrogenic H(+)-ATPase, and an increase in a K(+) conductance. Knockout mutants of known K(+) (tok, trk, trm-8, hak-1) and Cl(-) (a clc-3 homologue) channels and transporters had no effect on the transient depolarization, but trk and hak-1 do play a role in osmoadaptation, as does a homologue of a serine kinase regulator of H(+)-ATPase in yeast, Ptk2.


Asunto(s)
Potenciales de la Membrana , Neurospora crassa/fisiología , Presión Osmótica , Permeabilidad de la Membrana Celular , Regulación Fúngica de la Expresión Génica , Técnicas de Inactivación de Genes , Hifa/enzimología , Hifa/crecimiento & desarrollo , Transporte Iónico/fisiología , Neurospora crassa/enzimología , Neurospora crassa/genética , Neurospora crassa/crecimiento & desarrollo , Consumo de Oxígeno , Potasio/metabolismo , ATPasas de Translocación de Protón/metabolismo , Protones , Factores de Tiempo
14.
J Exp Bot ; 58(12): 3475-81, 2007.
Artículo en Inglés | MEDLINE | ID: mdl-17898420

RESUMEN

Voltage dependence of ionic currents and ion fluxes in a walled, turgor-regulating cell were measured in Neurospora crassa. The hyphal morphology of the model organism Neurospora simplifies cable analysis of ionic currents to determine current density for quantitative comparisons with ion fluxes. The ion fluxes were measured directly and non-invasively with self-referencing ion-selective microelectrodes. Four ions (H(+), Ca(2+), K(+), and Cl(-)) were examined. H(+) net uptake and Ca(2+) net release were small (10.2 nmol m(-2) s(-1) and 1.1 nmol m(-2) s(-1), respectively) and voltage independent. K(+) and Cl(-) fluxes were larger and voltage dependent. Maximal K(+) net release ( approximately 1440 nmol m(-2) s(-1)) was observed at positive voltages (+15 mV), while maximal Cl(-) net release ( approximately 905 nmol m(-2) s(-1)) was observed at negative voltage (-210 mV). A possible function of the net outward K(+) and Cl(-) fluxes is regulation of the plasma membrane potential. Total ion fluxes were 37-58% of the total ionic current density (about +/-244 mA m(-2), equivalent to +/-2500 nmol m(-2) s(-1), at 0 mV and -200 mV) so other ions must contribute significantly to the ionic currents.


Asunto(s)
Neurospora crassa/metabolismo , Transporte Iónico , Electrodos de Iones Selectos , Microelectrodos
15.
Microbiology (Reading) ; 153(Pt 5): 1530-1537, 2007 May.
Artículo en Inglés | MEDLINE | ID: mdl-17464067

RESUMEN

The internal hydrostatic pressure (turgor) of fungal cells is maintained at 400-500 kPa. The turgor is regulated by changes in ion flux and by production of the osmotically active metabolite glycerol. In Neurospora crassa, there are at least two genetically distinct pathways that function in adaptation to hyperosmotic shock. One involves a mitogen-activated protein (MAP) kinase cascade (kinases OS-4, OS-5 and OS-2 downstream of the osmosensing OS-1); the other is less understood, but involves the cut gene, which encodes a putative phosphatase. This study examined turgor regulation, electrical responses, ion fluxes and glycerol accumulation in the cut mutant. Turgor recovery after hyperosmotic treatment was similar to that in the wild-type, for both time-course ( approximately 40 min) and magnitude. Prior to turgor recovery, the hyperosmotic shock caused a rapid transient depolarization of the membrane potential, followed by a sustained hyperpolarization that occurred concomitant with increased H(+) efflux, indicating that the plasma membrane H(+)-ATPase was being activated. These changes also occurred in the wild-type. Net fluxes of Ca(2+) and Cl(-) during turgor recovery were similar to those in the wild-type, but K(+) influx was attenuated in the cut mutant. The similar turgor recovery can be explained by the ion uptake, since glycerol did not accumulate in the cut mutant within the time frame of turgor recovery (but did accumulate in the wild-type). The results suggest that turgor regulation involves multi-faceted coordination of both ion flux and glycerol accumulation. Ion uptake is activated by a MAP kinase cascade, while CUT is required for glycerol accumulation.


Asunto(s)
Presión Hidrostática , Neurospora crassa/fisiología , Equilibrio Hidroelectrolítico/fisiología , Calcio/metabolismo , Cloruros/metabolismo , Glicerol/metabolismo , Transporte Iónico/fisiología , Potenciales de la Membrana/fisiología , Proteínas Quinasas Activadas por Mitógenos/fisiología , Mutación , Neurospora crassa/enzimología , Neurospora crassa/genética , ATPasas de Translocación de Protón/fisiología , Equilibrio Hidroelectrolítico/genética
16.
Fungal Genet Biol ; 43(2): 65-74, 2006 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-16455272

RESUMEN

Hyphal tip-growing organisms have a high density of tip-localized mitochondria which maintain a membrane potential based on Rhodamine 123 fluorescence, but do not produce ATP based on the absence of significant oxygen consumption. Two possible roles of these mitochondria in tip growth were examined: Calcium sequestration and biogenesis, because tip-high cytoplasmic calcium gradients are a common feature of tip-growing organisms, and the volume expansion as the tip extends would require a continuous supply of additional mitochondria. Co-localization of calcium-sensitive fluorescent dye and mitochondria-specific fluorescent dyes showed that the tip-localized mitochondria do contain calcium, and therefore, may function in calcium clearance from the cytoplasm. Short-term inhibition of DNA synthesis or mitochondrial protein synthesis did not affect either tip growth, or mitochondrial shape or distribution. Therefore, mitochondrial biogenesis may not occur from the tip-localized mitochondria in hyphal organisms.


Asunto(s)
Calcio/metabolismo , Hifa/crecimiento & desarrollo , Mitocondrias/fisiología , Neurospora crassa/crecimiento & desarrollo , Colorantes Fluorescentes/metabolismo , Hifa/metabolismo , Procesamiento de Imagen Asistido por Computador , Potenciales de la Membrana , Microscopía Fluorescente , Mitocondrias/metabolismo , Morfogénesis , Neurospora crassa/metabolismo , Biogénesis de Organelos , Rodamina 123/metabolismo
17.
Eukaryot Cell ; 5(3): 480-7, 2006 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-16524903

RESUMEN

Fungi normally maintain a high internal hydrostatic pressure (turgor) of about 500 kPa. In response to hyperosmotic shock, there are immediate electrical changes: a transient depolarization (1 to 2 min) followed by a sustained hyperpolarization (5 to 10 min) prior to turgor recovery (10 to 60 min). Using ion-selective vibrating probes, we established that the transient depolarization is due to Ca(2+) influx and the sustained hyperpolarization is due to H(+) efflux by activation of the plasma membrane H(+)-ATPase. Protein synthesis is not required for H(+)-ATPase activation. Net K(+) and Cl(-) uptake occurs at the same time as turgor recovery. The magnitude of the ion uptake is more than sufficient to account for the osmotic gradients required for turgor to return to its original level. Two osmotic mutants, os-1 and os-2, homologs of a two-component histidine kinase sensor and the yeast high osmotic glycerol mitogen-activated protein (MAP) kinase, respectively, have lower turgor than the wild type and do not exhibit the sustained hyperpolarization after hyperosmotic treatment. The os-1 mutant does not exhibit all of the wild-type turgor-adaptive ion fluxes (Cl(-) uptake increases, but net K(+) flux barely changes and net H(+) efflux declines) (os-2 was not examined). Both os mutants are able to regulate turgor but at a lower level than the wild type. Our results demonstrate that a MAP kinase cascade regulates ion transport, activation of the H(+)-ATPase, and net K(+) and Cl(-) uptake during turgor regulation. Other pathways regulating turgor must also exist.


Asunto(s)
Calcio/metabolismo , Cloruros/metabolismo , Proteínas Quinasas Activadas por Mitógenos/fisiología , Neurospora crassa/fisiología , Potasio/metabolismo , ATPasas de Translocación de Protón/metabolismo , Calcio/análisis , Cloruros/análisis , Activación Enzimática , Transporte Iónico , Mutación , Neurospora crassa/enzimología , Neurospora crassa/genética , Neurospora crassa/crecimiento & desarrollo , Presión Osmótica , Técnicas de Placa-Clamp , Potasio/análisis
18.
Microbiology (Reading) ; 151(Pt 8): 2685-2692, 2005 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-16079346

RESUMEN

Mass flow of cytoplasm in Neurospora crassa trunk hyphae was directly confirmed by injecting oil droplets into the hyphae. The droplets move in a manner similar to cytoplasmic particles and vacuoles within the hyphae. The direction of mass flow is towards the growing hyphal tips at the colony edge. Based on flow velocities (about 5 microm s(-1)), hyphal radius and estimates of cytoplasm viscosity, the Reynolds number is about 10(-4), indicating that mass flow is laminar. Therefore, the Poiseulle equation can be used to calculate the pressure gradient required for mass flow: 0.0005-0.1 bar cm(-1) (depending on the values used for septal pore radius and cytoplasmic viscosity). These values are very small compared to the normal hydrostatic pressure of the hyphae (4-5 bar). Mass flow stops after respiratory inhibition with cyanide, or creation of an extracellular osmotic gradient. The flow is probably caused by internal osmotic gradients created by differential ion transport along the hyphae. Apical cytoplasm migrates at the same rate as tip extension, as do oil droplets injected near the tip. Thus, in addition to organelle positioning mediated by molecular motors, pressure-driven mass flow may be an integral part of hyphal extension.


Asunto(s)
Citoplasma/metabolismo , Hifa/crecimiento & desarrollo , Neurospora crassa/crecimiento & desarrollo , Neurospora crassa/citología , Presión
19.
Plant Physiol ; 134(1): 352-60, 2004 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-14730070

RESUMEN

To assess the role of the vacuole in responses to hyperosmotic and hypo-osmotic stress, the electrical properties of the vacuole were measured in situ. A double-barrel micropipette was inserted into the vacuole for voltage clamping. A second double-barrel micropipette was inserted into the cytoplasm to provide a virtual ground that separated the electrical properties of the vacuole from those of the plasma membrane. Osmotic stress causes immediate electrical responses at the plasma membrane (Lew RR [1996] Plant Physiol 97: 2002-2005) and ion flux changes and turgor recovery (Shabala SN, Lew RR [2002] 129: 290-299) in Arabidopsis root cells. In situ, the vacuole also responds rapidly to changes in extracellular osmotic potential. Hyperosmotic treatment caused a very large increase in the ionic conductance of the vacuole. Hypo-osmotic treatment did not affect the vacuolar conductance. In either case, the vacuolar electrical potential was unchanged. Taken in concert with previous studies of changes at the plasma membrane, these results demonstrate a highly coordinated system in which the vacuole and plasma membrane are primed to respond immediately to hyperosmotic stress before changes in gene expression.


Asunto(s)
Arabidopsis/fisiología , Membrana Celular/fisiología , Electrofisiología , Potenciales de la Membrana , Presión Osmótica , Técnicas de Placa-Clamp , Raíces de Plantas/fisiología , Vacuolas/fisiología
20.
Plant Physiol ; 129(1): 290-9, 2002 May.
Artículo en Inglés | MEDLINE | ID: mdl-12011359

RESUMEN

Hyperosmotic stress is known to significantly enhance net uptake of inorganic ions into plant cells. Direct evidence for cell turgor recovery via such a mechanism, however, is still lacking. In the present study, we performed concurrent measurements of net ion fluxes (with the noninvasive microelectrode ion flux estimation technique) and cell turgor changes (with the pressure-probe technique) to provide direct evidence that inorganic ion uptake regulates turgor in osmotically stressed Arabidopsis epidermal root cells. Immediately after onset of hyperosmotic stress (100/100 mM mannitol/sorbitol treatment), the cell turgor dropped from 0.65 to about 0.25 MPa. Turgor recovery started within 2 to 10 min after the treatment and was accompanied by a significant (30-80 nmol m-2 s-1) increase in uptake of K+, Cl-, and Na+ by root cells. In most cells, almost complete (>90% of initial values) recovery of the cell turgor was observed within 40 to 50 min after stress onset. In another set of experiments, we combined the voltage-clamp and the microelectrode ion flux estimation techniques to show that this process is, in part, mediated by voltage-gated K+ transporters at the cell plasma membrane. The possible physiological significance of these findings is discussed.


Asunto(s)
Arabidopsis/fisiología , Epidermis de la Planta/fisiología , Raíces de Plantas/fisiología , Transporte Biológico/efectos de los fármacos , Membrana Celular/metabolismo , Cloruros/metabolismo , Diseño de Equipo , Iones/metabolismo , Manitol/farmacología , Potenciales de la Membrana/fisiología , Presión Osmótica , Técnicas de Placa-Clamp , Potasio/metabolismo , Canales de Potasio con Entrada de Voltaje/metabolismo , Sodio/metabolismo , Sorbitol/farmacología , Estrés Mecánico
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA