RESUMEN
G-protein-coupled inwardly rectifying potassium channels (GIRKs) are important for regulation of synaptic transmission and neuronal firing rates. Because of their key role in brain function, we asked if these potassium channels are targets of alcohol action. Ethanol enhanced function of cerebellar granule cell GIRKs coupled to GABAB receptors. Enhancement of GIRK function by ethanol was studied in detail using Xenopus oocytes expressing homomeric or heteromeric channels. Function of all GIRK channels was enhanced by intoxicating concentrations of ethanol, but other, related inwardly rectifying potassium channels were not affected. GIRK2/IRK1 chimeras and GIRK2 truncation mutants were used to identify a region of 43 amino acids in the carboxyl (C) terminus that is critical for the action of ethanol on these channels.
Asunto(s)
Etanol/farmacología , Proteínas de Unión al GTP Heterotriméricas/metabolismo , Canales de Potasio de Rectificación Interna , Canales de Potasio/metabolismo , Transducción de Señal/efectos de los fármacos , Alcoholes/química , Alcoholes/farmacología , Animales , Calcio/metabolismo , Calcio/farmacología , Células Cultivadas , Cerebelo/citología , Cerebelo/efectos de los fármacos , Cerebelo/metabolismo , Canales de Potasio Rectificados Internamente Asociados a la Proteína G , Agonistas de Receptores GABA-B , Antagonistas de Receptores de GABA-B , Humanos , Potenciales de la Membrana , Oocitos/metabolismo , Potasio/metabolismo , Potasio/farmacología , Canales de Potasio/química , Canales de Potasio/genética , Inhibidores de Proteínas Quinasas , Proteínas Quinasas/metabolismo , Ratas , Ratas Sprague-Dawley , Receptores de GABA-B/metabolismo , Receptores Opioides mu/genética , Receptores Opioides mu/metabolismo , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Eliminación de Secuencia/genética , Xenopus laevisRESUMEN
Selective variations in cerebral GABAA receptor pharmacology and function are observed in experimental animals subjected to a number of alcohol-treatment and -withdrawal paradigms, and where human alcoholics with and without a range of concomitant diseases are compared with non-alcoholic cases. Recombination studies have shown that variations in GABAA receptor pharmacology and function can result from altering its subunit isoform composition. This commentary examines the rôle of subunit isoform expression in the response to long-term alcohol administration in animals, and in the pathogenesis of alcoholism-related brain damage in human cases.
Asunto(s)
Alcoholismo/metabolismo , Daño Encefálico Crónico/metabolismo , Etanol/efectos adversos , Regulación de la Expresión Génica/efectos de los fármacos , Receptores de GABA-A/efectos de los fármacos , Alcoholismo/complicaciones , Animales , Daño Encefálico Crónico/etiología , Modelos Animales de Enfermedad , Humanos , Receptores de GABA-A/genéticaRESUMEN
Human alcoholics have reduced neuronal counts in certain brain regions, such as superior frontal cortex (SFC), where the form and quantity of synaptic gamma-aminobutyric acid type A (GABAA) receptor sites are atypical. We measured the expression of GABAA receptor isoform mRNA and protein, since GABAA receptor pharmacology is strongly influenced by its subunit composition. Cortex samples were obtained at autopsy; whole-tissue extracts were assayed for mRNA by quantitative reverse transcriptase polymerase chain reaction (RT-PCR), while synaptic membranes were studied for both GABAA receptor pharmacology and subunit protein levels by Western blots with isoform-specific antibodies. Although alpha 1 and alpha 3 mRNA species were strongly expressed in alcoholics irrespective of cirrhosis than in controls, alpha 1 protein differed little between case groups, and alpha 3 protein showed some complex variations. Differences in GABAA pharmacology conformed more closely with differences in protein levels than with altered mRNA expression.
Asunto(s)
Alcoholismo/fisiopatología , Encéfalo/fisiopatología , Receptores de GABA-A/fisiología , Alcoholismo/patología , Unión Competitiva , Encéfalo/patología , Cadáver , Muerte Celular/fisiología , Humanos , Cirrosis Hepática Alcohólica/metabolismo , Piridinas/metabolismo , ARN Mensajero/metabolismo , Receptores de GABA-A/genética , Receptores de GABA-A/metabolismo , ZolpidemRESUMEN
The expression of the alpha 1, alpha 2 and alpha 3 isoforms of the GABAA receptor was studied in the superior frontal and motor cortices of 10 control, 10 uncomplicated alcoholic and 7 cirrhotic alcoholic cases matched for age and post-mortem delay. The assay was based on competitive RT/PCR using a single set of primers specific to the alpha class of isoform mRNA species, and was normalized against a synthetic cRNA internal standard. The assay was shown to be quantitative for all three isoform mRNA species. Neither the patient's age nor the post-mortem interval significantly affected the expression of any isoform in either cortical area. The profile of expression was shown to be significantly different between the case groups, particularly because alpha 1 expression was raised in both groups of alcoholics of controls. The two groups of alcoholics could be differentiated on the basis of regional variations in alpha 1 expression. In frontal cortex, alpha 1 mRNA expression was significantly increased when uncomplicated alcoholics were compared with control cases whereas alcoholic-cirrhotic cases were not significantly different from either controls or uncomplicated alcoholic cases. In the motor cortex, alpha 1 expression was elevated only when alcoholic-cirrhotic cases were compared with control cases. There was no significant difference between case groups or areas for any other isoform.
Asunto(s)
Alcoholismo/metabolismo , Corteza Cerebral/química , Receptores de GABA-A/química , Receptores de GABA-A/genética , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Southern Blotting , Corteza Cerebral/metabolismo , Cartilla de ADN , Femenino , Expresión Génica/fisiología , Humanos , Isomerismo , Masculino , Persona de Mediana Edad , Sondas de Oligonucleótidos , Reacción en Cadena de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa/normas , Cambios Post Mortem , ARN Mensajero/análisis , Estándares de ReferenciaRESUMEN
The displacement of [3H]flunitrazepam by unlabelled flunitrazepam or zolpidem was used to assess the affinity and density of sub-types of GABA(A) receptors in the superior frontal and primary motor cortices of ten alcoholic, seven alcoholic-cirrhotic and ten matched control cases. The binding was best fitted by a model with a single site for flunitrazepam, but two sites for zolpidem. Neither the patients' age nor the post-mortem interval were significantly correlated with the affinity or density of any of the binding sites. The affinity of all ligands did not differ either between cortical regions or across case groups. Hence, the density of each binding site was analyzed at constant affinity. The densities of flunitrazepam and high-affinity zolpidem binding sites were invariant across cortical regions and case groups. Low-affinity zolpidem binding sites were significantly more dense in the frontal than in the motor cortex of alcoholic cases irrespective of cirrhosis, whereas this regional difference was not significant in control cases.
Asunto(s)
Alcoholismo/metabolismo , Encéfalo/metabolismo , Cirrosis Hepática Alcohólica/metabolismo , Piridinas/metabolismo , Receptores de GABA-A/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Sitios de Unión , Femenino , Flunitrazepam/metabolismo , Lóbulo Frontal/metabolismo , Humanos , Modelos Lineales , Masculino , Persona de Mediana Edad , Corteza Motora/metabolismo , Cambios Post Mortem , ARN Mensajero/biosíntesis , Regulación hacia Arriba , ZolpidemRESUMEN
The GABAA receptor is the site of action of the inhibitory neurotransmitter GABA, as well as a number of pharmacologically important drugs such as benzodiazepines, barbiturates, and ethanol. The GABAA receptor is a pentameric complex composed of distinct polypeptides, which have been divided into five subunit classes on the basis of sequence homology. To date, 17 isoforms of the receptor have been identified and cloned in mammalian brain, and designated alpha 1-6, beta 1-4, gamma 1-4, delta and rho 1-2. In addition, several isoforms exist in alternatively spliced forms (for review see ref.). Studies on recombinant receptors have revealed that receptors constituted from different isoforms exhibit distinct pharmacological properties. For example, the alpha subunit class appears to be responsible for GABA enhancement of benzodiazepine binding. GABAA receptor function is modulated by benzodiazepine agonists such as flunitrazepam and diazepam, barbiturates, anaesthetics, neurosteroids, and ethanol. Chronic treatment of animals with many of these compounds can bring about profound changes in receptor expression and pharmacology. The RT/PCR assay described here was developed to quantify the alpha 1, alpha 2 and alpha 3 isoforms in the same assay. The amount of each isoform was quantified on the basis of a standard curve generated under identical PCR conditions to the target sequences. In this way it is possible to quantify multiple samples in each RT/PCR assay, thereby reducing inter-assay variability. The assay can be applied to quantify the expression of these isoforms in response to acute and chronic drug administration, or in particular disease states. Altered expression may reflect a corresponding change in protein synthesis, or an alteration of the subtype composition of GABAA receptor.
Asunto(s)
Encéfalo/metabolismo , Reacción en Cadena de la Polimerasa/métodos , Receptores de GABA-A/metabolismo , Humanos , Isomerismo , Transcripción GenéticaAsunto(s)
Depresores del Sistema Nervioso Central/farmacología , Etanol/metabolismo , Canales de Potasio de Rectificación Interna , Canales de Potasio/genética , Canales de Potasio/metabolismo , Secuencia de Aminoácidos , Animales , Análisis Mutacional de ADN/métodos , Femenino , Canales de Potasio Rectificados Internamente Asociados a la Proteína G , Ratones , Datos de Secuencia Molecular , Ratas , Xenopus laevisRESUMEN
Human alcoholics have reduced neuronal counts in certain brain regions, such as superior frontal cortex (SFC), where the form and quantity of synaptic γ-aminobutyric acid type A (GABAA) receptor sites are atypical. We measured the expression of GABAA receptor isoform mRNA and protein, since GABAA receptor pharmacology is strongly influenced by its subunit composition. Cortex samples were obtained at autopsy; whole-tissue extracts were assayed for mRNA by quantitative reverse transcriptase polymerase chain reaction (RT-PCR), while synaptic membranes were studied for both GABAA receptor pharmacology and subunit protein levels by Western blots with isoform-specific antibodies. Although α1 and α3 mRNA species were more strongly expressed in alcoholics irrespective of cirrhosis than in controls, α1 protein differed little between case groups, and α3 protein showed some complex variations. Differences in GABAA pharmacology conformed more closely with differences in protein levels than with altered mRNA expression.
RESUMEN
BACKGROUND: General anesthetics differentially affect various families of potassium channels, and some potassium channels are suggested to be potential targets for anesthetics and alcohols. METHODS: The voltage-gated (ERG1, ELK1, and KCNQ2/3) and inwardly rectifying (GIRK1/2, GIRK1/4, GIRK2, IRK1, and ROMK1) potassium channels were expressed in Xenopus oocytes. Effects of volatile agents [halothane, isoflurane, enflurane, F3 (1-chloro-1,2,2-trifluorocyclobutane), and the structurally related nonimmobilizer F6 (1,2-dichlorohexafluorocyclobutane)], as well as intravenous (pentobarbital, propofol, etomidate, alphaxalone, ketamine), and gaseous (nitrous oxide) anesthetics and alcohols (ethanol and hexanol) on channel function were studied using a two-electrode voltage clamp. RESULTS: ERG1, ELK1, and KCNQ2/3 channels were either inhibited slightly or unaffected by concentrations corresponding to twice the minimum alveolar concentrations or twice the anesthetic EC50 of volatile and intravenous anesthetics and alcohols. In contrast, G protein-coupled inwardly rectifying potassium (GIRK) channels were inhibited by volatile anesthetics but not by intravenous anesthetics. The neuronal-type GIRK1/2 channels were inhibited by 2 minimum alveolar concentrations of halothane or F3 by 45 and 81%, respectively, whereas the cardiac-type GIRK1/4 channels were inhibited only by F3. Conversely, IRK1 and ROMK1 channels were completely resistant to all anesthetics tested. Current responses of GIRK2 channels activated by mu-opioid receptors were also inhibited by halothane. Nitrous oxide (approximately 0.6 atmosphere) slightly but selectively potentiated GIRK channels. Results of chimeric and multiple amino acid mutations suggest that the region containing the transmembrane domains, but not the pore-forming domain, may be involved in determining differences in anesthetic sensitivity between GIRK and IRK channels. CONCLUSIONS: G protein-coupled inwardly rectifying potassium channels, especially those composed of GIRK2 subunits, were inhibited by clinical concentrations of volatile anesthetics. This action may be related to some side effects of these agents.
Asunto(s)
Anestésicos Generales/farmacología , Proteínas de Unión al GTP/metabolismo , Canales de Potasio de Rectificación Interna , Canales de Potasio/efectos de los fármacos , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Anestésicos por Inhalación/farmacología , Animales , Quimera , Electrofisiología , Canales de Potasio Rectificados Internamente Asociados a la Proteína G , Proteínas de Unión al GTP/genética , Activación del Canal Iónico/efectos de los fármacos , Datos de Secuencia Molecular , Óxido Nitroso/farmacología , Oocitos/efectos de los fármacos , Oocitos/metabolismo , Técnicas de Placa-Clamp , Canales de Potasio/genética , Canales de Potasio/metabolismo , ARN Complementario/biosíntesis , ARN Complementario/aislamiento & purificación , Ratas , Receptores Opioides/efectos de los fármacos , XenopusRESUMEN
Antibodies were raised against specific peptides from N-terminal regions of the alpha1 and alpha3 isoforms of the GABA(A) receptor, and used to assess the relative expression of these proteins in the superior frontal and primary motor cortices of 10 control, nine uncomplicated alcoholic and six cirrhotic alcoholic cases were matched for age and post-mortem delay. The regression of expression on post-mortem delay was not statistically significant for either isoform in either region. In both cortical areas, the regression of alpha1 expression on age differed significantly between alcoholic cases, which showed a decrease, and normal controls, which did not. Age had no effect on alpha3 expression. The alpha1 and alpha3 isoforms were found to be expressed differentially across cortical regions and showed a tendency to be expressed differentially across case groups. In cirrhotic alcoholics, alpha1 expression was greater in superior frontal than in motor cortex, whereas this regional difference was not significant in controls or uncomplicated alcoholics. In uncomplicated alcoholics, alpha3 expression was significantly lower in superior frontal than in motor cortex. Expression of alpha1 was significantly different from that of alpha3 in the superior frontal cortex of alcoholics, but not in controls. In motor cortex, there were no significant differences in expression between the isoforms in any case group.
Asunto(s)
Alcoholismo/metabolismo , Lóbulo Frontal/metabolismo , Corteza Motora/metabolismo , Péptidos/metabolismo , Receptores de GABA-A/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Envejecimiento , Secuencia de Aminoácidos , Animales , Anticuerpos/inmunología , Anticuerpos/metabolismo , Western Blotting , Células CHO , Cricetinae , Femenino , Humanos , Cirrosis Hepática Alcohólica/metabolismo , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Neuronas/metabolismo , Péptidos/química , Péptidos/inmunología , Isoformas de Proteínas , Subunidades de Proteína , Conejos , Receptores de GABA-A/química , Receptores de GABA-A/genética , Receptores de GABA-A/inmunología , Análisis de Regresión , Alineación de Secuencia , TransfecciónRESUMEN
An emerging idea is that long-term alcohol abuse results in changes in gene expression in the brain and that these changes are responsible at least partly for alcohol tolerance, dependence and neurotoxicity. The overall goal of our research is to identify genes which are differentially expressed in the brains of well-characterized human alcoholics as compared with non-alcoholics. This should identify as-yet-unknown alcohol-responsive genes, and may well confirm changes in the expression of genes which have been delineated in animal models of alcohol abuse. Cases were carefully selected and samples pooled on the basis of relevant criteria; differential expression was monitored by microarray hybridization. The inherent diversity of human alcoholics can be exploited to identify genes associated with specific pathological processes, as well as to assess the effects of concomitant disease, severity of brain damage, drinking behavior, and factors such as gender and smoking history. Initial results show selective changes in gene expression in alcoholics; of particular importance is a coordinated reduction in genes coding for myelin components.
Asunto(s)
Alcoholismo/genética , Análisis de Secuencia por Matrices de Oligonucleótidos/normas , Trastornos Relacionados con Alcohol/etiología , Trastornos Relacionados con Alcohol/genética , Perfilación de la Expresión Génica/métodos , Perfilación de la Expresión Génica/normas , HumanosRESUMEN
BACKGROUND: Changes in brain gene expression are thought to be responsible for the tolerance, dependence, and neurotoxicity produced by chronic alcohol abuse, but there has been no large scale study of gene expression in human alcoholism. METHODS: RNA was extracted from postmortem samples of superior frontal cortex of alcoholics and nonalcoholics. Relative levels of RNA were determined by array techniques. We used both cDNA and oligonucleotide microarrays to provide coverage of a large number of genes and to allow cross-validation for those genes represented on both types of arrays. RESULTS: Expression levels were determined for over 4000 genes and 163 of these were found to differ by 40% or more between alcoholics and nonalcoholics. Analysis of these changes revealed a selective reprogramming of gene expression in this brain region, particularly for myelin-related genes which were down-regulated in the alcoholic samples. In addition, cell cycle genes and several neuronal genes were changed in expression. CONCLUSIONS: These gene expression changes suggest a mechanism for the loss of cerebral white matter in alcoholics as well as alterations that may lead to the neurotoxic actions of ethanol.