Blue Light for Inactivation of Meatborne Pathogens and Maintaining the Freshness of Beef.

ABSTRACT: Beef is rich in various nutrients but easily spoils due to bacterial contamination; thus, a bactericidal method is needed to inactivate meatborne pathogens while maintaining the freshness of beef. The present study was conducted to investigate for the first time the bactericidal effect of blue light (BL) at 415 nm against four meatborne pathogens (methicillin-resistant Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes) both in vitro and inoculated onto the surface of fresh beef. The populations of the four pathogens on the nonirradiated control beef did not change significantly (P > 0.05), whereas a dose-dependent inactivation effect was found for BL-treated beef both in vitro and in vivo. On the beef cuts, BL at 109.44 J/cm2 inactivated 90% of inoculated cells of the tested strains (P < 0.05), and this inactivation effect was sustained during 7 days of cold storage. Insignificant changes in lipid oxidation rate, water holding capacity, and cooking loss were found during storage between the control beef and the beef irradiated at 109.44 J/cm2 at the same time. BL had a minor and nonsignificant effect on surface color and free amino acid concentrations. The pH of the treated beef increased more slowly (P < 0.05) than did that of untreated beef. These results suggest that BL could be a novel bactericide and could help maintain the freshness of beef.

Understanding a defensive response of methicillin-resistant Staphylococcus aureus after exposure to multiple cycles of sub-lethal blue light.

Blue light (BL) has shown bactericidal effectiveness against methicillin-resistant Staphylococcus aureus (MRSA), one of the major clinical pathogens with antibiotic resistance. Bacteria likely respond to the oxidative stress induced by BL; however, the defensive response is still unclear. This study aimed to reveal the phenotypic change in MRSA after being exposed to 15 cycles of sub-lethal BL illumination. The comparative transcriptomic results showed that the expression of peptidoglycan (PG) synthesis gene glmS was significantly upregulated in the cells after the multiple cycle light treatment, and the biochemical analysis determined that the content of PG synthesized was increased by 25.86% when compared with that in control cells. Furthermore, significant thickening of the cell wall was observed under a transmission electron microscope (P < .05). The light sensitivity of the tested MRSA strain was reduced after the multiple cycle light treatment, indicating the possibility of MRSA being more adaptive to the BL stress. The present study suggested that multiple cycles of sub-lethal BL could change the light susceptibility of MRSA through thickening the cell wall.