RESUMEN
Many animal and plant pathogenic bacteria employ a type three secretion system (T3SS) to deliver type three effector proteins (T3Es) into host cells. Efficient secretion of many T3Es in the plant pathogen Xanthomonas campestris pv. campestris (Xcc) relies on the global chaperone HpaB. However, how the domain of HpaB itself affects effector translocation/secretion is poorly understood. Here, we used genetic and biochemical approaches to identify a novel domain at the C-terminal end of HpaB (amino acid residues 137-160) that contributes to virulence and hypersensitive response (HR). Both in vitro secretion assay and in planta translocation assay showed that the secretion and translocation of T3E proteins depend on the C-terminal region of HpaB. Deletion of the C-terminal region of HpaB did not affect binding to T3Es, self-association or interaction with T3SS components. However, the deletion of C-terminal region sharply reduced the mounts of free T3Es liberated from the complex of HpaB with the T3Es, a reaction catalyzed in an ATP-dependent manner by the T3SS-associated ATPase HrcN. Our findings demonstrate the C-terminal domain of HpaB contributes to disassembly of chaperone-effector complex and reveal a potential molecular mechanism underpinning the involvement of HpaB in secretion of T3Es in Xcc.
Asunto(s)
Regulación Bacteriana de la Expresión Génica , Chaperonas Moleculares/metabolismo , Sistemas de Secreción Tipo III/metabolismo , Xanthomonas campestris/metabolismo , Proteínas Bacterianas/metabolismo , Transporte de ProteínasRESUMEN
The lytic transglycosylases (LTs) are important enzymes that degrade peptidoglycan of the bacterial cell wall and affect many biological functions. We present here that XC_0706 and XC_3001 are annotated as the LTs in Xanthomonas campestris pv. campestris. XC_0706 is associated with virulence and plays a pivotal role in cell division. Mutation on XC_3001 reduced hypersensitive response induction and the translocation of type III effector, but did not affect the function of the type II secretion system. Further studies showed that multiple LTs genes contribute to efficiency of the type III secretory system in X. campestris pv. campestris.
Asunto(s)
Proteínas Bacterianas/metabolismo , Glicosiltransferasas/metabolismo , Sistemas de Secreción Tipo III/metabolismo , Xanthomonas campestris/enzimología , Proteínas Bacterianas/genética , Capsicum/microbiología , Regulación Bacteriana de la Expresión Génica , Glicosiltransferasas/genética , Enfermedades de las Plantas/microbiología , Sistemas de Secreción Tipo III/genética , Virulencia , Xanthomonas campestris/genética , Xanthomonas campestris/fisiologíaRESUMEN
The development of a type of carbon-nanotube-promoted Co-Cu catalyst, which displays excellent performance for highly effective and selective formation of the C(2-4)-oxygenates, especially BuOH and DME, from syngas, is reported.