Surfactant-Free Method to Prevent Gold Nanoparticle Aggregation and Its Surface-Enhanced Raman Scattering Applications.

The introduction of surfactants to stabilize colloidal citrate-reduced gold nanoparticles (prevent aggregation) is usually used in surface-enhanced Raman scattering (SERS) applications. However, the surfactants have many drawbacks for SERS applications, such as increasing the SERS background and blocking surface active sites. Here, we develop a surfactant-free method to stabilize colloidal cit-AuNPs based on alkali regulation, and this method can prevent gold nanoparticle aggregation under different harsh treatments, including ligand modification, centrifugation-based washing/enrichment, and salt addition. The SERS spectra, density functional theory simulation, and ζ potentials of cit-AuNPs indicate that the stability of enhanced cit-AuNPs under alkaline conditions is attributed to both the increased negative charge density (by ∼6 times from pH 7 to 12) and the molecular configuration on the metal surface. Compared with surfactant-based methods, this method can well maintain the inherent optical and interface properties of nanoparticles, avoid the SERS background, and avoid blocking of the surface active site due to the presence of surfactants. This method will enable AuNPs to have a wide range of applications in areas such as highly sensitive SERS sensors.

Complement Coercion in Mandarin Chinese: Evidence from a Self-paced Reading Study.

The study aims to explore the processing pattern of Mandarin Chinese sentences with complement coercion. Complement coercion is a known linguistic phenomenon in which some verbs, semantically requiring an event-denoting complement, are combined with an entity-denoting complement, as in Mary began the book. The combination (i.e., event-selecting verb + entity-denoting noun) has been reported to involve type mismatch, and thus elicits processing difficulty. While the phenomenon has been extensively studied in Indo-European languages, such as English and German, it is debatable if the phenomenon exists in a typologically distinct language from English (e.g., in structural complexity of words), such as Mandarin. To provide empirical evidence, the study conducted a self-paced reading experiment to compare the processing patterns of coercion sentences and non-coercion controls in Mandarin. The results showed longer reading times for the coercion sentences than the non-coercion counterparts, which supported previous findings about the processing difficulty of complement coercion.

Application of life cycle assessment for municipal solid waste management options in Hohhot, People's Republic of China.

With increasing population and urbanization levels in the People's Republic of China, environmental problems related to the management of municipal solid waste (MSW) are inevitable. This study aimed to determine the environmental impact of the current MSW management system in Hohhot City and to establish an optimum future strategy for it by applying life cycle assessment (LCA) methodology. Four scenarios were compared using the CML-IA impact characterization method, which took into account their potential contribution to global warming, ozone depletion, human toxicity, photochemical ozone creation, acidification, and eutrophication potentials. The system boundaries included the collection and recycling, transfer and transportation of MSW, and its disposal by incineration, landfilling, and carbon dioxide (CO2) capture methods. The results showed that the scenario involving landfill and incineration in a ratio of 1:5 was the optimal waste management option; however, increasing the proportion of waste incinerated led to a significant increase in global warming potential. Additional technologies are thus required to overcome this problem, and it was found that the use of CO2 capture technology resulted in a 30% reduction in the total environmental impact potential. This study's results indicate that LCA is a valuable and practical tool to support decision-making that can be used to suggest problematic areas in current waste management strategies and to determine an optimal alternative to the solid waste management option.

Quality of Work Life, Nurses' Intention to Leave the Profession, and Nurses Leaving the Profession: A One-Year Prospective Survey.

PURPOSE: To examine the associations among quality of work life, nurses' intention to leave the profession, and nurses leaving the profession. DESIGN: A prospective study design was used. METHODS: Participants were 1,283 hospital nurses with a purposive sampling in Taiwan. The self-reported questionnaire consisted of three questionnaires: the Chinese version of the Quality of Nursing Work Life scale, an intention-to-leave profession questionnaire, and a demographic questionnaire. Records of nurses leaving the profession were surveyed 1 year later. Data were analyzed by descriptive statistics and inferential statistics. FINDINGS: As many as 720 nurses (56.1%) had tendencies to leave their profession. However, only 31 nurses (2.5%) left their profession 1 year later. Nurses' intention to leave the profession mediated the relationship between the milieu of respect and autonomy, quality of work life, and nurses leaving the profession. CONCLUSIONS: The milieu of respect and autonomy describing the quality of work life predicts the nurses' intention to leave the profession, and together these predict nurses leaving the profession. CLINICAL RELEVANCE: This study illustrates that nurse managers could provide effective interventions to ameliorate the milieu of respect and autonomy aspect of quality of work life to prevent nurses from leaving their profession.

[Testing the Reliability and Validity of the Taiwan Health-Related Quality of Life for Nausea and Vomiting During Pregnancy Scale].

BACKGROUND: Symptom distress related to pregnancy-related nausea and vomiting is known to impact quality of life in pregnant women. However, few reliable and valid assessment tools are available for research use. PURPOSE: To test the reliability and validity of the Taiwan health-related quality of life for nausea and vomiting during pregnancy (NVPQOL) scale. METHODS: A cross-sectional study design was conducted. A convenience sample of 416 pregnant women in their first and second trimesters were recruited from the prenatal clinics of one medical center and one regional teaching hospital in southern Taiwan. Chi-square tests, item analysis, and principal axis factor analysis were used to examine construct validity. Pearson's correlation coefficient was then conducted to compare the concurrent validity of the scale against the Chinese-language version of the World Health Organization Quality of Life Assessment [WHOQOL-BREF (TW)]. RESULTS: Four factors were extracted using factor analysis: "limitations" (10 items), "physical symptoms/aggravating factors" (9 items), "emotions" (6 items), and "fatigue" (4 items), which together accounted for 67.45% of the total variance. The NVPQOL showed good convergent (r = .78 ~ .85, p < .01), discriminate (r = .48 - .68, p < .01), and concurrent validities, with a -0.37 correlation coefficient between the WHOQOL-BREF (TW) and the NVPQOL (p < .01). The Cronbach's α of the NVPQOL was .95. The intraclass correlation coefficient for test-retest reliability of two weeks was .92 (n = 37, p < .001). CONCLUSIONS / IMPLICATIONS FOR PRACTICE: The NVPQOL may be used in future research and clinical assessment to measure quality of life in women who experience nausea and vomiting during pregnancy in order to provide appropriate nursing interventions in a timely manner to improve quality of life.

[A project to improve the completion rate of discharge-planning services for organ-transplant patients].

BACKGROUND & PROBLEMS: The average score for satisfaction with self-care instructions among transplant patients was 84 out of 100 in a 2010 survey of patient satisfaction with discharge-planning services. This score was significantly below the hospital average of 90. In addition, discharged patients made more frequent telephone inquiries to healthcare providers to ask questions about post-organ-transplantation self-care. A follow-up study targeted a sample of 30 discharged transplantation patients using hospital medical record reviews and telephone inquiries. This survey found a transplant instruction completion rate of 75% during discharge preparation and 69% accuracy among participants in answering self-care knowledge questions. Both figures were lower than expected. PURPOSE: The goal of this project was to increase the instruction completion rate from 75% to 100% and the percentage of correct answers to self-care knowledge questions self-care knowledge from 69% to 95%. RESOLUTIONS: This study integrated all relevant nursing information into a standardized discharge-planning service booklet that was given to inpatients for reference. Furthermore, a self-care checklist was developed and introduced. Finally, a standard procedure for delivering nursing instructions and a regular audit and follow-up monitoring system were established. RESULTS: The nurses' instruction completion rate rose to 100% and the percentage of questions on self-care knowledge correctly answered by patients increased to 98%. CONCLUSIONS: Adopting a patient-centered philosophy of care and a model of interdisciplinary collaboration effectively integrated resources and promotes nursing quality and quality of life for transplant patients. The project effectively improved the completing rate for post-transplant discharge-planning service instructions and enhanced the self-care knowledge of transplant patients.

Therapeutic Potential of Olfactory Ensheathing Cells and Adipose-Derived Stem Cells in Osteoarthritis: Insights from Preclinical Studies.

Olfactory-ensheathing cells (OECs) are known for their role in neuronal regeneration and potential to promote tissue repair. Adipose-derived stem cells (ADSCs), characterized by mesenchymal stem cell (MSC) traits, display a fibroblast-like morphology and express MSC surface markers, making them suitable for regenerative therapies for osteoarthritis (OA). In this study, OECs and ADSCs were derived from tissues and characterized for their morphology, surface marker expression, and differentiation capabilities. Collagenase-induced OA was created in 10-week-old C57BL/6 mice, followed by intra-articular injections of ADSCs (1 × 105), OECs (1 × 105), or a higher dose of OECs (5 × 105). Therapeutic efficacy was evaluated using rotarod performance tests, MRI, histology, and immunohistochemistry. Both cell types exhibited typical MSC characteristics and successfully differentiated into adipocytes, osteoblasts, and chondrocytes, confirmed by gene expression and staining. Transplantation significantly improved rotarod performance and preserved cartilage integrity, as seen in MRI and histology, with reduced cartilage destruction and increased chondrocytes. Immunohistochemistry showed elevated type II collagen and aggrecan in treated joints, indicating hyaline cartilage formation, and reduced MMP13 and IL-1ß expression, suggesting decreased inflammation and catabolic activity. These findings highlight the regenerative potential of OECs and ADSCs in treating OA by preserving cartilage, promoting chondrocyte proliferation, and reducing inflammation. Further research is needed to optimize delivery methods and evaluate long-term clinical outcomes.

The hiPSC-derived corneal endothelial progenitor-like cell recovers the rabbit model of corneal endothelial dystrophy.

INTRODUCTION: Corneal endothelial dysfunction results in cornea opacity, damaging sightedness, and affecting quality of life. A corneal transplant is the current effective intervention. Due to the scarcity of donated cornea, such an unmet medical need requires a novel therapeutic modality. OBJECTIVES: Customizing patients' corneal endothelial progenitor cells with proliferative activity and lineage restriction properties shall offer sufficient therapeutic cells for corneal endothelial dystrophy. METHODS: The customized induced human corneal endothelial progenitor-like cell (iHCEPLC) was obtained through cell fate conversions starting from PBMC (peripheral blood mononuclear cell), hiPSC (human induced pluripotent stem cell), and hNCC (human neural crest cell), while it finally reached the iHCEPLC state via a series of induction. Several molecular diagnoses were applied to depict its progenitor state, including RNAseq, FlowCytometer, immunostainings, and rtPCR. Significantly, it can be induced to gain differentiation maturity through contact inhibition. In addition, a BAK-mediated rabbit model of corneal endothelial dystrophy was established in the present study to test the therapeutic effectiveness of the iHCEPLC. RESULTS: After inducing cell fate conversion, the specific HCEC markers were detected by rtPCR and immunostaining in iHCEPLC. Further, RNAseq was applied to distinguish its progenitor-like cell fate from primary human corneal endothelial cells (HECE). FlowCytometry profiled the heterogeneity subpopulation, consistently displaying a subtle difference from primary HCEC. A terminal differentiation can be induced in iHCEPLC, addressing its progenitor-like fate. iHCEPLC can restore the BAK-based rabbit model of corneal endothelial dystrophy. Immunohistochemistry verified that such acuity restoration of the BAK-treated cornea is due to the introduced iHCEPLC, and such therapeutic effectiveness is observed in the long term. CONCLUSION: Here, we demonstrated that customized iHCEPLC has long-term therapeutic efficacy. As a progenitor cell, our iHCEPLC has a restricted cell lineage nature and can proliferate in vitro, supporting sufficient therapeutic candidate cells. Due to the immune-privileged nature of the cornea, our iHCEPLC proves the principle of therapeutical feasibility in both autogenic and allogeneic modalities.

Electrically driven green, olivine, and amber color nanopyramid light emitting diodes.

We report the fabrication and studies of electrically driven green, olivine, and amber color nanopyramid GaN light emitting diodes (LEDs). InGaN/GaN multiple quantum wells (MQWs) were grown on the nanopyramid semipolar facets. Compared with the commonly used (0001) c-plane MQWs, the semipolar facet has lower piezoelectric field, resulting in much faster radiative recombination efficiency. This is important for high In content MQWs. The measured internal quantum efficiencies for green, olivine, and amber color LED are 30%, 25%, and 21%, respectively. The radiative and non-radiative lifetime of the semipolar MQWs are also investigated.

Ultralow-background SERS substrates for reliable identification of organic pollutants and degradation intermediates.

Chemical methods for preparing SERS substrates have the advantages of low cost and high productivity, but the strong background signals from the substrate greatly limit their applications in characterization and identification of organic compounds. Herein, we developed a one-step synthesis method to prepare silver nanoparticle substrates with ultralow SERS background using anionic ligands as stabilizing agents and applied the SERS substrate for the reliable and reproducible identification of typical organic pollutants and corresponding degradation intermediates. The synthesis method shows excellent universality to different reducing agents cooperating with different anionic ligands (Cl-, Br-, I-, SCN-). As model applications, the machine learning algorithm can realize the precise prediction of six organophosphorus pesticides and eight sulfonamide antibiotics with 100% accuracy based on SERS training data. More importantly, the ultralow-background SERS substrate enables one to detect and identify the time-dependent degradation intermediates of organophosphorus pesticides by combining them with density functional theory (DFT) calculations. All the results indicate that the ultralow-background SERS substrate will greatly push the development of SERS characterization applications.

Size-controllable colloidal Ag nano-aggregates with long-time SERS detection window for on-line high-throughput detection.

Making metal nanoparticle aggregates is a common way to improve surface-enhanced Raman scattering (SERS) enhancement via the formation of hot spots between nanoparticles. Here, we propose a "freeze-thaw-ultrasonication" method to obtain stable colloidal Ag nano-aggregates (AgNAs) with controllable sizes, which can remain stable for a few days. Compared with other method using aggregation reagents (e.g., organic molecules and salt), this method can maintain metal surface charges and adsorption affinity, which ensures the excellent SERS stability and sensitivity. The SERS detection window during the experiment can reach more than 25 min, which makes it a prerequisite for accurate SERS detection during a long-time range. Combining the obtained stable AgNAs with microfluidic devices, we established a sequential SERS on-line continuous detection method for the high-throughput detection of multiplex samples. The UV-Fenton degradation process of methylene blue (MB) is continuously on-line monitored through this platform, which is more sensitive than the UV-Vis Spectrum. Moreover, we have realized the sensitive and accurate detection of 5-nitro-8-hydroxyquinoline (5-NQ) with antibacterial and anticancer activities based on chloride-functionalized silver, which paved a way for SERS high-throughput analysis in bioanalysis and other fields.

Lasing at exciton transition in optically pumped gallium nitride nanopillars.

We report the observation of room temperature lasing action in optically pumped GaN nanopillars. The nanopillars were fabricated by patterned etching and crystalline regrowth from a GaN substrate. When nanopillars were optically excited, a narrow emission peak emerged from the broad spontaneous emission background. The increasing rate is nine times faster than that of the spontaneous emission background, showing the onset of lasing action. The lasing occurs right at the center of spontaneous emission rather than the often reported redshifted wavelength. A spectroscopic ellipsometry analysis indicates that the gain of lasing action is provided by exciton transition.

Processing of Complement Coercion With Aspectual Verbs in Mandarin Chinese: Evidence From a Self-Paced Reading Study.

This study examines whether Chinese complement coercion sentences with aspectual verbs will elicit processing difficulty during real-time comprehension. Complement coercion is a linguistic phenomenon in which certain verbs (e.g., start, enjoy), requiring an event-denoting complement, are combined with an entity-denoting complement (e.g., book), as in The author started a book. Previous studies have reported that the entity-denoting complement elicited processing difficulty following verbs that require event argument compared with verbs that do not (e.g., The author wrote a book). While the processing of complement coercion has been extensively studied in Indo-European languages such as English and German, it is relatively under-researched in Sino-Tibetan languages such as Mandarin Chinese. Given the fact that there are many linguistic elements behaving distinctly in the different language families, for instance, verbs with respect to their semantic properties and syntactic representations of the complement, it is meaningful to investigate whether or not the existing linguistic differences have any effect on the processing of complement coercion in Mandarin. With this research goal, we recorded self-paced reading time of 61 native Mandarin speakers to investigate the processing of the entity-denoting complement in sentences with three different verb types (aspectual verbs which require an event-denoting complement, preferred verbs which denote a preferred interpretation of the aspectual expressions, and non-preferred verbs which denote a non-preferred but plausible interpretation of the aspectual expressions), as exemplified in // gù-kè kai-shǐ/tián-xie/chá-kàn zhè-fèn wèn-juàn "The customer started/filled in/checked the questionnaire." It was found that the entity noun complement (e.g., zhè-fèn wèn-juàn "the questionnaire") elicited significantly longer reading times in coercion sentences than non-coercion counterparts. The results are compatible with the previous findings in English that complement coercion sentences impose processing cost during real-time comprehension. The study contributes empirical evidence to coercion studies cross-linguistically.

Play Effects on Hospitalized Children With Acute Respiratory Infection: An Experimental Design Study.

Acute respiratory infection is a major health issue and a primary cause of morbidity and mortality among preschool-aged children worldwide. Disease and hospitalization are chief stressors for children during their development. Therapeutic play has been used in pediatric care processes and has been demonstrated to be effective by most studies that have targeted children undergoing surgeries or invasive medical treatments. Currently, few published studies have focused on children receiving acute inpatient care. Additionally, not all types of therapeutic play produce significant results, and few studies have elaborated on the purposes, principles, and concrete measures of therapeutic play. Therefore, this study aimed to design therapeutic play that would reduce the stress responses of preschool-aged children hospitalized with acute respiratory infection. An experimental design with a pretest/posttest was conducted with 105 preschool children recruited from a regional teaching hospital in southern Taiwan. The children in the experimental group received the therapeutic play, while those in the control group received routine nursing care. Children in the experimental group showed significantly greater reductions in their physiological, psychological, and behavioral stress responses than those in the control group. Future studies could apply therapeutic play to children from different age groups with diverse health issues before recommending it be used in pediatric health settings.

Unexpected identification of a bupivacaine analog from smuggling by using single-crystal X-ray diffraction analysis: N-(2,6-dimethylphenyl)-1-phenethylpiperidine-2-carboxamide.

The identification of new psychoactive substances (NPSs) is essential against drug abuse, especially for "new" drugs that are not regulated by international drug conventions. A suspicious powder seized by the officers of Taipei Customs Administration of Taiwan was sent to this laboratory for analysis by using gas chromatography-mass spectrometry (GCMS), liquid chromatography-high resolution mass spectrometry (LCHRMS), proton nuclear magnetic resonance (1H NMR), carbon-13 nuclear magnetic resonance (13C NMR) with distortionless enhancement by polarization transfer (DEPT) at proton pulses of 45°, 90°, and 135°, two-dimensional correlation NMR measurements (2D_COSY), and heteronuclear single-quantum correlation NMR measurements (2D_HSQC). However, the structure of this "unknown" sample was difficult to identify. Alternatively, single-crystal X-ray crystallography was applied for structural determination after the crystallization of the compound in methanol. The structure was thus identified as N-(2,6-dimethylphenyl)-1-phenethylpiperidine-2-carboxamide (NDMPPPC), an analog of bupivacaine with similar pharmacological effects to those of cocaine, ketamine and morphine. The identification of NDMPPPC is in accordance with all mass fragments and NMR signal data, demonstrating that single-crystal X-ray diffraction can be used for structural determination, especially for complicated structures of "new" drugs or "unknown" samples. The seizure of NDMPPPC from smuggling indicates a great potential to become part of the next generation of NPSs.

lncRNA TINCR facilities bladder cancer progression via regulating miR­7 and mTOR.

Long non­coding RNAs (lncRNAs) have been implicated in various human malignancies, but the molecular mechanism of lncRNA TINCR ubiquitin domain containing (TINCR) in bladder cancer remains unclear. The present study found that the expression of TINCR was significantly increased in bladder cancer tissues and cell lines, when compared with that in adjacent normal tissues and normal urinary tract epithelial cell line SV­HUC­1, respectively. Moreover, the high expression of TINCR was associated with tumor metastasis and advanced tumor, node, metastasis stage, as well as reduced overall survival rates of patients with bladder cancer. Further investigation revealed that microRNA (miR)­7 was negatively mediated by TINCR in bladder cancer cells. Silencing of TINCR expression significantly increased miR­7 expression and reduced bladder cancer cell proliferation, migration and invasion, while knockdown of miR­7 expression reversed the inhibitory effects of TINCR downregulation on bladder cancer cells. mTOR was then identified as a target gene of miR­7 in bladder cancer, and it was demonstrated that overexpression of mTOR reversed the inhibitory effects of miR­7 on bladder cancer cells. In conclusion, this study suggests that TINCR/miR­7/mTOR signaling may be a potential therapeutic target for bladder cancer.

Transformation of quality of life in prenatal women with nausea and vomiting.

PROBLEM: Nausea and vomiting not only cause physical discomfort in pregnant women but also impact their quality of life. BACKGROUND: Few longitudinal studies have been conducted to investigate QOL of women over the course of pregnancy. AIM: To examine the transformation of health-related QOL and related factors among pregnant women with NV during three trimesters. METHODS: A longitudinal research design with convenience sampling was used. A structural questionnaire was used to repeatedly measure the data of 101 pregnant women with NV during the first, second, and third trimesters. A generalized estimating equation (GEE) was used to analyze the collecting data. FINDINGS: The results showed significant differences in symptom distress, prenatal stress, and health-related QOL among the three trimesters in pregnant women with NV (p<0.001). The scores of symptom distress, prenatal stress, and health-related QOL in the first trimester were significantly higher than those in the second and third trimesters (p<0.001). The GEE indicated that the trimester of pregnancy, severity of NV, symptom distress, and prenatal stress were key factors for the transformation of health-related QOL of women with NV during pregnancy. DISCUSSION: The findings of this study are seminal in terms of understanding the relationships between symptom distress, prenatal stress and health-related QOL in pregnant women with NV over the course of a pregnancy. CONCLUSION: This study can serve as a reference for designing interventions (i.e., professional support) for women in different pregnancy stages to improve their health-related QOL during pregnancy.

Identification of a liver-specific cAMP response element in the human argininosuccinate synthetase gene.

Argininosuccinate synthetase (ASS), a key enzyme in the urea cycle, participates in many metabolic processes including arginine biosynthesis and the citrulline-nitric oxide (NO) cycle. Factors like diets, hormones and pro-inflammatory stimuli are known to regulate ASS gene expression primarily at the transcription level. However, little is known about the cis-elements for transcriptional regulation of the ASS gene. In this study, we employed DNase I hypersensitive sites mapping to identify potential regulatory sites of the gene and revealed a site located at 10 kb upstream of the transcription start site which is responsible for liver-specific cAMP induction. Furthermore, a cAMP response element (CRE) highly conserved among mammals was identified and was experimentally verified. Our results show that liver-specific enhancement of ASS gene expression is mediated in part by the cAMP signaling pathway through a distal CRE site.

pth moment exponential stability of stochastic memristor-based bidirectional associative memory (BAM) neural networks with time delays.

Stochastic memristor-based bidirectional associative memory (BAM) neural networks with time delays play an increasingly important role in the design and implementation of neural network systems. Under the framework of Filippov solutions, the issues of the pth moment exponential stability of stochastic memristor-based BAM neural networks are investigated. By using the stochastic stability theory, Itô's differential formula and Young inequality, the criteria are derived. Meanwhile, with Lyapunov approach and Cauchy-Schwarz inequality, we derive some sufficient conditions for the mean square exponential stability of the above systems. The obtained results improve and extend previous works on memristor-based or usual neural networks dynamical systems. Four numerical examples are provided to illustrate the effectiveness of the proposed results.

Cloning, identification, and functional analysis of bone marrow stromal cell antigen-2 from sika deer (Cervus nippon).

BST-2(tetherin/CD317/HM1.24) has been identified as a cellular antiviral factor that inhibits the release of a wide range of enveloped viruses from infected cells. Orthologs of BST-2 have been identified in several species including humans, monkeys, cows, sheep, pigs, and mice. In this study, we cloned the gene and characterized the protein of the BST-2 homolog from sika deer (Cervus nippon). cnBST-2 shares 37.8% and 74.2% identity with the BST-2 homologs from Homo sapiens and Ovis aries, respectively. The extracellular domain of cnBST-2 has two putative N-linked glycosylation sites and three potential dimerization sites. cnBST-2 was shown to be expressed on the cell surface, like human BST-2. Exogenous expression of cnBST-2 resulted in potent inhibition of HIV-1 particle release in 293T cells; however, this activity resisted antagonism by HIV-1 Vpu. Moreover, cnBST-2 was not able to activate nuclear factor-κB, in contrast to human BST-2. This study is the first report of the isolation and characterization of BST-2 from C. nippon.