Coupling of biostimulation and bioaugmentation for enhanced bioremoval of chloroethylenes and BTEX from clayey soil.

The bioremoval potential of Pseudomonas plecoglossicida toward mixed contaminants was explored through the coupled biostimulation and bioaugmentation in soil microcosm. Response surface methodology was employed to optimize nutrients and innoculum size for the cometabolic removal of two representative chloroethylenes, trichloroethylene (TCE) and cis-1,2-dichloroethylene (cis-DCE), mixed with benzene, toluene, ethylbenzene, and xylenes (BTEX). The interactive effects of nutrients [nitrogen (N) and phosphorus (P)] and inoculum size toward the bioremoval of mixture of BTEX (600 mg kg-1), cis-DCE (10 mg kg-1), and TCE (10 mg kg-1) were estimated using principal component analysis and two-dimensional hierarchical cluster analysis. The optimal condition was confirmed with C:N:P ratio of 100:26.7:1.8-4.8 and higher inoculum size (≥25%), where 97.7% of benzene, 98.3% of toluene, 91.2% of ethylbenzene, 45.6% of m,p-xylene, 31.2% of o-xylene, 26.9% of cis-DCE, and 33.5% of TCE were bioremoved.

Zinc and copper supplements enhance trichloroethylene removal by Pseudomonas plecoglossicida in water.

The effects of two microelements, zinc and copper, on the aerobic co-metabolic removal of trichloroethylene (10 mg/L) by the isolate Pseudomonas plecoglossicida were investigated. The strain was previously isolated from a petroleum-contaminated site using toluene (150 mg/L) as substrate. Different concentrations (1, 10 and 100 mg/L) of microelements provided with SO42- and Cl- were tested. The results showed the supplement of Zn2+ and Cu2+ at the low concentration (1 mg/L) significantly enhanced cell growth. The removal efficiencies for toluene and trichloroethylene were also enhanced at the low concentration (1 mg/L) of Zn2+ and Cu2+. Compared to the control without zinc supplement, higher concentrations of zinc (10 and 100 mg/L) enhanced the removal efficiencies for both toluene and trichloroethylene in the first three days but showed some inhibitory effect afterward. However, the higher concentrations of Cu2+ (10 and 100 mg/L) always showed inhibitory to the toluene removal while showing inhibitory to the TCE removal after three days. For both Zn2+ and Cu2+, the anions SO42- and Cl- did not show significant difference in their effects on the toluene removal. A possible mechanism for Zn2+ and Cu2+ to enhance the removal of toluene and trichloroethylene would be their involvement in toluene oxygenase-based transformation processes. In addition, high concentrations of Zn2+ and Cu2+ ions could be removed from the liquid by the cells accordingly. The results imply a potential of supplementing low concentrations of zinc and copper to enhance bioremediation of the sites co-contaminated with toluene and trichloroethylene.