Umbilical cord MSC-derived exosomes improve alveolar macrophage function and reduce LPS-induced acute lung injury.

Acute lung injury (ALI) is a severe condition that can progress to acute respiratory distress syndrome (ARDS), with a high mortality rate. Currently, no specific and compelling drug treatment plan exists. Mesenchymal stem cells (MSCs) have shown promising results in preclinical and clinical studies as a potential treatment for ALI and other lung-related conditions due to their immunomodulatory properties and ability to regenerate various cell types. The present study focuses on analyzing the role of umbilical cord MSC (UC-MSC))-derived exosomes in reducing lipopolysaccharide-induced ALI and investigating the mechanism involved. The study demonstrates that UC-MSC-derived exosomes effectively improved the metabolic function of alveolar macrophages and promoted their shift to an anti-inflammatory phenotype, leading to a reduction in ALI. The findings also suggest that creating three-dimensional microspheres from the MSCs first can enhance the effectiveness of the exosomes. Further research is needed to fully understand the mechanism of action and optimize the therapeutic potential of MSCs and their secretome in ALI and other lung-related conditions.

208 W single-frequency 1064 nm laser based on a single-crystal fiber master-oscillator power amplifier.

High-power all-solid-state continuous-wave (CW) single-frequency laser with high linear polarization is a significant source for quantum optics and precision measurement. In this Letter, a high-power linearly polarized CW single-frequency laser based on the single-crystal fiber (SCF) master-oscillator power amplifier (MOPA) is presented, in which a homemade 140 W low-noise CW single-frequency laser and a Nd:YAG SCF are firstly employed as the seed laser and the medium of the MOPA, respectively. The mode-matching between the pump laser propagated with waveguide form and the freely propagated seed laser is optimized by considering the influence of the degradations of the polarization and the beam quality. Finally, when the incident powers of the pump and seed lasers are 262.6 W and 126.3 W, respectively, the seed waist radius is optimized to 200 µm. In this case, the output power of the linearly polarized laser reaches up to 208 W, which is the highest output power, to the best of our knowledge. The presented results provide a good reference for implementing a high power and high degree of the polarization and good beam quality laser based on the SCF MOPA.

High-average-power single-frequency pulse optical parametric oscillator based on pulse-integrated seed-injection automatic locking.

Near-infrared nanosecond (ns) single-longitudinal-mode (SLM) pulse light generated from an optical parametric oscillator (OPO) is an important source in nonlinear optics and high-precision spectral analysis. In this Letter, a stable SLM near-infrared ns pulse light source generated from the OPO is presented, which is achieved by developing a seed-injection automatic locking technique based on a pulse-integrated photodetector (PIPD). Depending on the PIPD, the peak power of the pulse light detected by the photodiode is converted to the average power by integrating several pulses. As a result, the detector saturation is thoroughly eliminated, and the interference signal including the resonance point between seed and pulse lights can easily be attained by scanning the resonator length. On this basis, a microcontroller unit (MCU) is employed to realize automatic locking by looking for the minimum value of the interference signal. Finally, a SLM 824 nm pulse light source with an output power of 20.5 W and a linewidth of 51.42 MHz is obtained. The presented method can pave the way to implement a low-cost and compact high-average-power SLM pulse OPO.

High-power single-frequency continuous-wave 355 nm UV laser via a frequency-correlated dual-wavelength laser.

An all-solid-state single-frequency continuous-wave (CW) 355 nm ultraviolet (UV) laser based on a dispersion-compensated doubly resonant resonator is presented in this Letter that is achieved by employing homemade high-stability all-solid-state frequency-correlated dual-wavelength lasers at 1064 and 532 nm and a temperature-controlled type-I critical-phase-matching LiB3O5 (LBO) to act as the fundamental laser source and the nonlinear medium, respectively. The frequency-correlated dual-wavelength single-frequency CW laser supplies the fundamental frequency 1064 and 532 nm lasers with good frequency synchronization. And the temperature-controlled LBO acts as the dispersion-compensation element to realize double resonance of the 1064 and 532 nm laser. Finally, a 4.2 W high-stability 355 nm UV laser is experimentally obtained, and the corresponding total conversion efficiency is up to 20.5%. To the best of our knowledge, this is the highest power reported about single-frequency CW 355 nm UV laser. The presented method can pave a way to develop a compact single-frequency 355 nm UV laser with high output power.

Realization of CW single-frequency tunable Ti:sapphire laser with immunity to the noise of the pump source.

All-solid-state continuous-wave (CW) single-frequency tunable Ti:sapphire (Ti:S) laser is an important source in quantum optics and atomic physics. However, intracavity etalon (IE) locking is easily influenced by the intensity noise of the pump source in the low frequency band. In order to address this issue, a differential detector with dual-photodiodes (PDs) is designed and employed in the experiment. Both PDs are used to detect the lights of the pump source and the built Ti:S laser, respectively. As a result, the influence of the intensity noise of the pump source on the stability of the IE locking is successfully eliminated and the IE is stably locked to the oscillating longitudinal-mode of the laser. On this basis, a stable CW single-frequency tunable Ti:S laser is realized. The presented method is beneficial to attain a stable single-frequency tunable laser with immunity to the intensity noise of the pump source.

High-average-power single-longitudinal-mode pulse laser based on a pulse saturation seed-injection locking OPO.

A high-average-power and narrow-linewidth nanosecond (ns) pulse 824 nm laser is a crucial source for the generation of deep-ultraviolet (DUV) 248 nm laser by means of the sum-frequency process with the 354.5 nm laser. To this purpose, in this Letter, we present a seed-injection-locked high-average-power ns pulse single-longitudinal-mode (SLM) 824 nm laser. By developing a novel, to the best of our knowledge, pulse-saturated seed-injection locking method, disturbance of the pulse laser on the locking of the injected seed laser is successfully eliminated. As a result, the output power of 824 nm laser is up to 21.2 W at the incident pump power of 48.1 W, and the pulse width is 15 ns. Especially, the signal-to-noise ratio of the detected modulated sideband signal exceeds 28 dB, which ensures that the achieved linewidth of the 824 nm laser is as narrow as 38.8 MHz. These results demonstrate the potential of the proposed pulse saturation seed-injection locking OPO cavity for high-power and narrow-linewidth laser applications.

Auto-pump-depleted stable single-longitudinal-mode 1.5 µm source with the assistance of SHG.

To realize a stable single-longitudinal-mode (SLM) 1550-nm light source for the generation of non-classical states, a ring auto-pump-depleted singly resonant optical parametric oscillator (SRO) with the assistance of second-harmonic-wave generation (SHG) is designed and built in this Letter. A magnesium oxide doped periodically polarized lithium niobate (MgO:PPLN) crystal and a lithium triborate (LBO) crystal are employed as the optical parametric downconversion (OPDC) and SHG crystals, respectively. Especially, the introduced SHG can firstly increase the loss difference between the lasing and non-lasing modes so that the dual-mode or multi-mode coupling in the achieved SRO can be effectively eliminated and the stable SLM operation is achieved. At the same time, the SHG will automatically adjust the output coupling efficiency of SRO, so as to achieve efficient conversion efficiency and auto-pump depletion of SRO. In addition, due to the SHG, it is easy to achieve the low-intensity noise multi-wavelength output for the stable SLM SRO. As a result, the output powers of the SLM 1550 nm and 775 nm are up to 4.05 W and 3.25 W, respectively, and the total optical conversion of the built SRO can achieve 45.58%. The presented method paves a way to develop a compact stable SLM multi-wavelength SRO, and the obtained SRO is further beneficial to develop compact continuous-variable non-classical light fields.

Self-mode-matching compact low-noise all-solid-state continuous wave single-frequency laser with output power of 140 W.

The high power all-solid-state continuous wave single-frequency laser is a significant source for science and application due to good beam quality and low noise. However, the output power of the laser is usually restricted by the harmful thermal lens effect of the solid gain medium. To address this issue, we develop a self-mode-matching compact all-solid-state laser with a symmetrical ring resonator in which four end-pumped Nd:YVO4 laser crystals are used for both laser gain media and mode-matching elements. With this ingenious design, the thermal lens effect of every laser crystal can be controlled and the dynamic of the designed laser including the stability range and the beam waist sizes at crystals can be manipulated only by adjusting the pump power used on each laser gain medium. Under an appropriate combination of pump powers on four crystals, self-mode-matching in a resonator is realized. A stable CW single-frequency at 1064 nm with 140-W power, 102-kHz linewidth, and low intensity noise is obtained. The presented design paves an effective way to further scale-up the output power of a compact laser by employing more pieces of gain media.

Optimization of the nonlinear crystal length for high-power single-frequency intracavity frequency-doubling lasers.

We report a method of optimizing the nonlinear crystal length of the intracavity frequency-doubling laser, which is achieved by maximizing the output power of the frequency-doubling laser in the case of ensuring the single longitudinal mode (SLM) operation of the laser. The optimal length of the nonlinear crystal for an SLM oscillation of the intracavity frequency-doubling laser is firstly theoretically predicted by comparing the losses introduced by the nonlinear crystal with different lengths with that of ensuring the SLM operation of the laser. Then three nonlinear LiB3O5 (LBO) crystals with the length of 18, 20, and 22 mm are adopted to be the frequency-doubling components in the experiment. By recording the output power and monitoring the longitudinal mode structure of the laser, it is found that the nonlinear LBO crystal with the length of 20 mm is the best candidate, since the output power is higher than that of the LBO crystal with the length of 18 mm, and the SLM operation of the laser is readily achieved compared to that of the LBO crystal with the length of 22 mm. The experimental results well agree with the theoretical predictions. The current method can pave a good way to attain a single-frequency continuous-wave intracavity frequency-doubling laser.

Realization of compact Watt-level single-frequency continuous-wave self-tuning titanium: sapphire laser.

Here, we present a compact Watt-level single-frequency continuous-wave (CW) self-tuning titanium:sapphire (Ti:S) laser, which is implemented using a three-plate Ti:S crystal as both a gain medium and frequency-tuning element. The thickness ratio of the three-plate Ti:S crystal is 1:2:4, of which the thinnest plate measured 1 mm. The optical axes lie on their own surfaces and parallel to each other. Based on the presented self-tuning crystal, a ring resonator is designed and built. The maximum wavelength tuning range of the single-frequency self-tuning Ti:S laser is 108.84 nm, as demonstrated experimentally by rotating the three-plate Ti:S crystal, indicating good agreement with theoretical prediction. To the best of our knowledge, this is the first study to report a single-frequency CW self-tuning Ti:S laser, which can provide a feasible approach for achieving a compact all-solid-state single-frequency CW-tunable Ti:S laser.

Diving angle optimization of BRF in a single-frequency continuous-wave wideband tunable titanium:sapphire laser.

In this study, the optimal condition of a multi-plate birefringent filter (BRF) used in a single-frequency continuous-wave (CW) tunable laser is theoretically and experimentally investigated. The dependence of the optimal condition on the diving angle of the BRF optical axis is first deduced. Based on the proposed optimal condition, the diving angle of the BRF optical axis is optimized to 29.1°. Subsequently, a novel off-axis multi-plate BRF with a thickness ratio of 1:2:5:9 and the thinnest plate of 0.5 mm is designed and utilized in a tunable titanium:sapphire (Ti:S) laser. As a result, the operating wavelength of the Ti:S laser is successfully tuned from 691.48 to 995.55 nm by rotating the BRF 18°. The obtained tuning slope efficiency and maximum tuning range are 16.9 nm/° and 304.07 nm, respectively. The experimental results agree well with the theoretical analysis results, which provide a feasible approach for designing BRFs to satisfy the requirements of other single-frequency CW wideband tunable lasers.

Alterations of coagulation and fibrinolysis in patients with blunt splenic injury after splenic artery embolization.

BACKGROUND: Thrombotic complications following splenectomy have been documented. However, there has been sparse literature regarding thrombotic complications following splenic artery embolization (SAE).The objective of this study was to determine changes in coagulation and fibrinolysis and assess the thrombotic risk after SAE in patients with blunt splenic injury (BSI). METHODS: This study included 38 BSI patients who were hemodynamically stable on admission. SAE was performed if the splenic injury was classed as grade III or greater and had no requirement of immediate surgery. Platelet (PLT), fibrinogen (FIB), D-dimers (D-D), fibrinogen/fibrin degradation products (FDP), antithrombin III (AT III), prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), hemoglobin (Hb), and hematocrit (Hct) were measured before SAE procedures and then 1d, 3d, and 7d after SAE. RESULTS: The technical success rate of SAE and the splenic salvage rate were 100%. There was no mortality. Compared with pre-SAE values, the levels of PLT, FIB, D-D, and FDP increased significantly at 3 days and 7 days after SAE (p < 0.05). However, AT III, PT, APTT, TT, Hb, and Hct showed no statistically significant difference at 1d, 3d, and 7d after SAE (p > 0.05). CONCLUSION: Alterations in PLT and hemostatic parameters might contribute to the increased risk of thrombotic complications in BSI patients undergoing SAE. Thromboembolism following SAE should be considered and thrombotic prophylaxis should be recommended.

Influence of the pump scheme on the output power and the intensity noise of a single-frequency continuous-wave laser.

The influence of the pump scheme on the intensity noise of the single-frequency continuous-wave (CW) laser is investigated in this paper, which is implemented in a single-frequency CW Nd:YVO4 1064 nm laser by comparing the traditional 808 nm pumping scheme (TPS) to the direct 888 nm pumping scheme (DPS). Under the conditions that the lasers with TPS and DPS have the same cavity structure and the cavity mirrors, as well as the same operation state including the thermal lens of the laser crystals and the mode-matching between the pump laser mode and the laser cavity mode at the laser crystals, the output power of the laser with DPS is up-to 32.0 W, which is far higher than that of 21.1 W for the laser with TPS. However, the intensity noise of the DPS laser including resonant relaxation oscillation (RRO) frequency of 809 kHz, RRO peak amplitude of 31.6 dB/Hz above the shot noise level (SNL) and the SNL cutoff frequency of 4.2 MHz, respectively, is also higher than that of 606 kHz, 20.4 dB/Hz and 2.4 MHz for the TPS laser. After further analyses, we find that the laser crystal with high doping concentration and long optical length is employed for DPS laser in order to improve the pump laser absorption efficiency, which can simultaneously increase the dipole coupling between the active atoms and the laser cavity, and then results in a high RRO frequency with a large amplitude peak as well as a high SNL cutoff frequency of the laser.

Improvement of the intensity noise and frequency stabilization of Nd:YAP laser with an ultra-low expansion Fabry-Perot cavity.

Continuous-wave, single-frequency, solid-state lasers with long-term frequency stability and low-intensity noise are an essential resource to generate squeezed and entangled states of light. In order to obtain the stable, nonclassical states of light, the frequency of the laser has to be stabilized with a stable reference. Due to the zero expansion property at a certain temperature, an ultra-low expansion (ULE) Fabry-Perot (F-P) cavity with a high finesse can be used as one of the best candidates of the frequency reference. We perform a detailed analysis of an extraordinarily high-frequency stability and ultra-low-intensity noise laser based on an improved cascade Pound-Drever-Hall frequency stabilization to a ULE F-P cavity. The frequency drift of the laser is suppressed to 7.72 MHz in 4 hours, and the noise level of the laser is simultaneously reduced to the quantum noise limit in the frequency below 300 kHz, which provides the possibility for the direct generation of a stable, high-level squeezed state in a lower-frequency region.

Intensity noise suppression of a high-power single-frequency CW laser by controlling the stimulated emission rate.

The intensity noise of a high-power single-frequency continuous-wave (CW) laser is very harmful for applications in precise measurements and quantum communication. By simply lengthening the length of a laser resonator to decrease the stimulated emission rate of the laser, the coupling strength of all noise sources in the resonant laser field will be reduced, and thus the intensity noise of the output laser will be prominently suppressed. Based on theoretical analyses of the laser noise spectra, we experimentally implement a low-noise high-power single-frequency laser with a 1050 mm long resonator. With the assistance of an intracavity imaging system and nonlinear second-harmonic generation, the amplitude of the resonant relaxation oscillation peak and the shot-noise level (SNL) cutoff frequency are successfully reduced to 8.6 dB/Hz above SNL and 1.0 MHz, respectively, under the output power of 16 W. The work provides an effective way to develop a high-quality laser with high output power and low intensity noise.

Continuously tunable single-frequency 455 nm blue laser for high-state excitation transition of cesium.

A continuously tunable high-power single-frequency 455 nm blue laser for high-state excitation transition 6S1/22↔7P3/22 of Cs atoms was presented in this Letter, which was implemented by an intracavity frequency-doubled Ti:sapphire laser with an LBO crystal. The highest output power of 1.0 W was attained under a pump power of 13.5 W with an optical conversion efficiency of 7.4%. The measured power stability in 3 h and beam quality were better than ±0.27% (peak-to-peak) and Mx2=1.58, My2=1.18, respectively. By continuously scanning the length of the resonator after locking the employed intracavity etalon to the oscillating longitudinal mode of the laser, the continuous tuning range of the 455 nm blue laser was up to 32 GHz and was mode hop free. Lastly, the whole saturation absorption spectrum of the higher state excitation transition 6S1/22(Fg=3)↔P3/22(Fe=2,3,4) and 6S1/22(Fg=4)↔7P3/22(Fe=3,4,5) of Cs133 was successfully observed in the experiment, which further verified the excellent performance of the 455 nm blue laser.

Investigation about the influence of longitudinal-mode structure of the laser on the relative intensity noise properties.

The influence of the longitudinal-mode structure (LMS) of the laser on the relative intensity noise (RIN) properties was investigated in this paper after an all-solid-state continuous-wave (CW) single-frequency 1064 nm laser with output power of 50.3 W was achieved. The LMS of the laser was manipulated by controlling the temperature of the nonlinear lithium triborate (LBO) crystal deliberately introduced to the resonator. When the laser worked with single-longitudinal-mode (SLM) operation, the stable RIN spectrum was observed and measured. Moreover, with the decrease of the nonlinear conversion efficiency (NCE), the frequency and amplitude of the resonant-relaxation oscillation (RRO) peak regularly shifted to the higher frequencies and increased, respectively. However, with further decrease of the NCE, the laser began to work with the multi-longitudinal-mode (MLM) or mode-hopping operation and the unstable RIN spectra of the laser were both observed not only at low frequencies but also at high frequencies. Once the NCE was moved away, the MLM or mode-hopping can only enhance the fluctuation of the laser RIN spectrum at the low frequencies (lower than the frequency of RRO). The experimental results directly revealed the relationship between the LMS of the laser and the RIN spectra, and confirmed definitely that the key to achieve a stable high power laser with low intensity noise was to realize single-frequency operation of the laser with free MLM and mode-hopping.

Realization of a 101 W single-frequency continuous wave all-solid-state 1064 nm laser by means of mode self-reproduction.

We realized a 101 W single-frequency continuous wave (CW) all-solid-state 1064 nm laser by means of mode self-reproduction in this Letter. Two identical laser crystals were placed into a resonator to relax the thermal lens of the laser crystals, and an imaging system was employed to realize cavity mode self-reproduced at the places of the laser crystals. Single-frequency operation of the resonator was realized by employing a new kind of high extinction ratio optical diode based on the terbium scandium aluminum garnet crystal to realize a stable unidirectional operation of the laser, together with introducing a large enough nonlinear loss to the resonator to effectively suppress the multi-mode oscillation and mode hopping of the laser. As a result, a 101 W single-frequency 1064 nm laser in a single-ring resonator was achieved with 42.3% optical efficiency. The measured power stability for 8 h and the beam quality were better than ±0.73% and 1.2, respectively.

Investigation on the thermal characteristic of MgO:PPSLT crystal by transmission spectrum of a swept cavity.

A method of evaluating the thermal focal length of nonlinear crystal via transmission spectrum of a swept cavity (TSSC) is presented. By recording the resonant point offset of the TSSC, the thermal focal length can be successfully measured. Furtherly, by distinguishing the absorption of ultraviolet (UV) laser and UV laser induced infrared absorption (ULIIRA), it is clear that the ULIIRA is the important factor which induces the thermal lens effect compared to the absorption of UV laser for MgO-doped periodically poled stoichiometric lithium tantalate (MgO:PPSLT) crystal and it becomes serious with the increase of the generated UV laser. The ULIIRA coefficient measurement and thermal focal length evalution of MgO:PPSLT crystal can supply an useful reference for researchers to generate high quality UV laser and squeezed or entangled state of optical field by using MgO:PPSLT crystal. The presented method can also be used to precisely evaluate the thermal focal length of other nonlinear crystals.

Self-injection locked CW single-frequency tunable Ti:sapphire laser.

A self-injection locked continuous-wave (CW) single-frequency tunable Ti:sapphire laser is demonstrated in this paper. Unidirectional operation of the presented Ti:sapphire laser is achieved by using a retro-reflecting device which can retro-reflect a seed laser beam from one direction back into the counter-propagating field. On the basis, the influence of the transmission of output coupler on the unidirectional operation is investigated and it is found that stable unidirectional and single-frequency operation of the Ti:sapphire laser is achieved when the loss difference between both output directions is larger than a certain value, which is easy to be realized by choosing the transmission of output coupler. When the output coupler with transmission of 6.5% is utilized, the maximal 5 W CW single-frequency Ti:sapphire laser with stable unidirectional operation is obtained with the pump power of 18 W. The measured power stability and M2 are better than ±0.9% and 1.1, respectively. The maximal tuning range and continuous frequency-tuning ability are 120 nm and 40.75 GHz, respectively.