Plasma aryl hydrocarbon receptor associated with epicardial adipose tissue in men: a cross-sectional study.

BACKGROUND: Epicardial adipose tissue (EAT) is a type of ectopic fat with endocrine and paracrine functions. Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that responds to environmental stimuli. AhR expression is associated with obesity. In this cross-sectional study, we aimed to determine the relationship between circulating AhR concentrations and EAT. METHODS: A total of 30 men with obesity and 23 age-matched men as healthy controls were enrolled. Plasma AhR concentrations were determined at fasting. The EAT thickness was measured on the free wall of the right ventricle from the basal short-axis plane by magnetic resonance imaging. RESULTS: The participants with obesity had a higher plasma AhR level than the controls (81.0 ± 24.5 vs. 65.1 ± 16.4 pg/mL, P = 0.010). The plasma AhR level was positively correlated with EAT thickness (correlation coefficient = 0.380, P = 0.005). After adjusting for fasting glucose levels, plasma AhR levels were still significantly associated with EAT thickness (95% CI 0.458‒5.357, P = 0.021) but not with body mass index (P = 0.168). CONCLUSION: Plasma AhR concentrations were positively correlated with EAT thickness on the free wall of the right ventricle in men. Further investigations are needed to evaluate the causal effects and underlying mechanisms between AhR and EAT.

Circulating platelet concentration is associated with bone mineral density in women.

In this cross-sectional study, enrollment included 818 female adults undergoing bone mineral density (BMD) assessment during the health examination. Subjects with osteoporosis had the lowest circulating platelet concentrations. The circulating platelet concentration was positively correlated with BMD. A high platelet concentration had independently low odds of osteoporosis. PURPOSE: Platelets play an important role in bone metabolism. However, the association between circulating platelet counts and bone mineral density (BMD) has been inconsistently reported. We aimed to investigate the relationship between platelet counts and osteoporosis in Chinese women. METHODS: In this cross-sectional study, a total of 818 female adults who underwent BMD assessment during the health examination were enrolled. Blood cell counts and biochemistry data were recorded. RESULTS: Subjects with osteoporosis had the lowest platelet counts (238 ± 59 × 109/L) compared with subjects with osteopenia (256 ± 64 × 109/L) and a normal BMD (269 ± 76 × 109/L, P < 0.001). The circulating platelet concentration was positively correlated with the BMD of the lumbar spine (r = 0.195, P < 0.001), left hip (r = 0.145, P < 0.001), and right hip (r = 0.149, P < 0.001). According to the receiver operating characteristic curve, the cutoff platelet concentration for differentiating osteoporosis was 260 × 109/L. A high platelet concentration had significantly low odds of osteoporosis after adjusting for other covariates (odds ratio = 0.574, 95% confidence interval: 0.346‒0.953, P = 0.032). CONCLUSION: The circulating platelet concentration was significantly correlated with BMD in Chinese women.

Bioavailability pathways underlying zinc-induced avoidance behavior and reproduction toxicity in Lumbricus rubellus earthworms.

We investigated possible bioavailability pathways underlying zinc-induced avoidance behavior and sublethal reproduction impairment in Lumbricus rubellus. Clay-loam (pH 7.3) and sandy soil (three pH values of 4.3-6.0) were amended with zinc sulfate at six soil concentrations of total Zn ranging from 0.1 to 36 mmol/kg dw. Estimated and measured concentrations of free and exchangeable Zn ranged 10(-4) to 7.1 mmol/l. Avoidance behavior responses were fast and could be directly predicted from the activity of free zinc ions without a modifying pH effect. The repellent effect is thus likely mediated by a direct action of Zn(2+) ions on epidermal chemosensitive receptors. Body zinc uptake, however, was determined by proton competition with free Zn(2+) sorption. Excess accumulation of body Zn was a good predictor of reproduction decline, which is indicative of internal zinc poisoning. The results indicated that zinc affects earthworms via both direct and indirect mechanisms of external and internal exposure.

Land use dynamics of the fast-growing Shanghai Metropolis, China (1979-2008) and its implications for land use and urban planning policy.

Through the integrated approach of remote sensing and geographic information system (GIS) techniques, four Landsat TM/ETM+ imagery acquired during 1979 and 2008 were used to quantitatively characterize the patterns of land use and land cover change (LULC) and urban sprawl in the fast-growing Shanghai Metropolis, China. Results showed that, the urban/built-up area grew on average by 4,242.06 ha yr(-1). Bare land grew by 1,594.66 ha yr(-1) on average. In contrast, cropland decreased by 3,286.26 ha yr(-1) on average, followed by forest and shrub, water, and tidal land, which decreased by 1,331.33 ha yr(-1), 903.43 ha yr(-1), and 315.72 ha yr(-1) on average, respectively. As a result, during 1979 and 2008 approximately 83.83% of the newly urban/built-up land was converted from cropland (67.35%), forest and shrub (9.12%), water (4.80%), and tidal land (2.19%). Another significant change was the continuous increase in regular residents, which played a very important role in contributing to local population growth and increase in urban/built-up land. This can be explained with this city's huge demand for investment and qualified labor since the latest industrial transformation. Moreover, with a decrease in cropland, the proportion of population engaged in farming decreased 13.84%. Therefore, significant socio-economic transformation occurred, and this would lead to new demand for land resources. However, due to very scarce land resources and overload of population in Shanghai, the drive to achieve economic goals at the loss of cropland, water, and the other lands is not sustainable. Future urban planning policy aiming at ensuring a win-win balance between sustainable land use and economic growth is urgently needed.

[Atmospheric correction of HJ-1 CCD data for water imagery based on dark object model].

The CCD multi-band data of HJ-1A has great potential in inland water quality monitoring, but the precision of atmospheric correction is a premise and necessary procedure for its application. In this paper, a method based on dark pixel for water-leaving radiance retrieving is proposed. Beside the Rayleigh scattering, the aerosol scattering is important to atmospheric correction, the water quality of inland lakes always are case II water and the value of water leaving radiance is not zero. So the synchronous MODIS shortwave infrared data was used to obtain the aerosol parameters, and in virtue of the characteristic that aerosol scattering is relative stabilized in 560 nm, the water-leaving radiance for each visible and near infrared band were retrieved and normalized, accordingly the remotely sensed reflectance of water was computed. The results show that the atmospheric correction method based on the imagery itself is more effective for the retrieval of water parameters for HJ-1A CCD data.

Rapid Urbanization and Implications for Flood Risk Management in Hinterland of the Pearl River Delta, China: The Foshan Study.

The purpose of this paper is to examine the linkage between rapid urbanization and flood risk in the hinterlands of the Pearl River Delta, P.R. China. Foshan, a typical hinterland city in the Pearl River Delta region, was selected as a case study. Land use and cover change in Foshan during 1988-2003 was analyzed using remote sensing and geographic information system (GIS) techniques. Furthermore, analysis on historical hydrological data during 1962-2005 was performed. Results show that rapid urbanization has resulted in losses of farmland, forest and shrub since 1988. In addition, in order to compensate or offset the loss of farmland due to rapid urban expansion, more than 30 % of the forest and 20 % of the shrub areas were transformed into farmlands. Inevitably, both the urban and agricultural lands increased the pressure on the drainage systems. Furthermore, over the past decades human activities such as dredging up the floodways, excavating sand and building water facilities in the rivers, significantly changed the hydrological conditions, and therefore impaired the rivers' capacity to buffer floods. Lessons from the Foshan case implied that, in addition to natural processes, human activities driven by socio-economic factors should be considered responsible for the recently increasing level of flood risks. Both economically and environmentally, it is irrational and impractical to encourage encroachment of lands vulnerable to floods. It is also realistic and urgent to effectively prevent and control the adverse ecological consequences of urbanization and economic activities for building their wealth and prominence.

Reduced apoptosis in newborn compared to adult rat intestine after ischemia-reperfusion injury.

The pathogenesis of necrotizing enterocolitis (NEC) is unknown. Ischemia and reperfusion (I/R) injury has been considered to be a major contributing factor. More recent reports have noted that apoptosis is a significant and perhaps the principal contributor to cell death after I/R. Some reports revealed that infants with NEC and perforated bowel can completely recover with drainage alone. This study aims to assess the ability of newborn rat intestine to resist apoptosis after I/R injury compared with adult rat intestine. Intestines from 10 groups of rats (n = 6 for each study group) were studied: (1) normal control group; (2) ischemia group, receiving vascular occlusion for 60 min; (3) I/R groups receiving vascular occlusion for 60 min and reperfusion for 15, 30, and 60 min, respectively. Apoptosis was quantified by TUNEL methods. Statistical analysis was performed using ANOVA with Dunn's test. TUNEL-positive cells per 10 crypts were significantly increased in the ischemia and I/R groups compared to the control group. The peak number of positive cells by TUNEL was recognized 30 min after reperfusion in adult and newborn rats and then reduced gradually. The newborn rats had significantly less TUNEL-positive cells per 10 crypts than adult rats subjected to I/R injury (p < 0.05). We demonstrated that the activation of apoptosis occurred after intestinal I/R injury, especially during the reperfusion phase. The newborn intestine was more resistant to I/R injury and thus may have significant clinical application.

Cranial repair using BMP-2 gene engineered bone marrow stromal cells.

BACKGROUND: Bone grafts, allografts, and biocompatible artificial bone substitutes all have their shortcomings when used for the repair of cranial bone defects. Tissue engineered bone shows promise as an alternative for the repair of these defects. MATERIALS AND METHODS: Rabbit bone marrow mesenchymal stromal cells (MSCs) were separated from iliac crest aspirates and expanded in a monolayer culture 1 month before implantation. These MSCs were then infected with replication-defective adenovirus-human BMP-2 genes 1 week before implantation. Bilateral critical-size cranial defects were created in the animal with removal of osteoinductive periosteum and dura. MSCs were mixed with alginate UP (ultrapure) to form MSC/polymer construct. MSCs used for the control site were infected with adenovirus beta-galactosidase (beta-gal). After 1 week, 6 weeks, and 3 months, five rabbits from each experimental group were sacrificed and the cranial defect site was examined by histology study. RESULTS: Near-complete repair of the large size cranial defects using the tissue engineered MSC/alginate construct was observed. The H&E stain and von Kossa's staining should better regenerate bone at the experiment site. A statistically significant difference in bone formation was noted by 3D CT imaging at 3 months post-BMP-2 treatment of the cranial defects (0.79 +/- 0.06 versus 0.47 +/- 0.05 cm(2), P < 0.001) but not at 6 weeks (0.36 +/- 0.04 versus 0.33 +/- 0.03 cm(2), P = 0.347). CONCLUSIONS: Near-complete repair of large cranial defects can be achieved using tissue engineered bone. The use of newly developed polymers as well as the integration of the stem cell concept with gene medicine is necessary to attain this goal.