[Diagnostic and prognostic significance of the hypoxia-dependent transcriptional mediator for the development of a carious lesion]. / Diagnosticheskaia i prognosticheskaia znachimost' gipoksiiazavisimogo transkriptsionnogo mediatora dlia razvitiia karioznogo porazheniia.

OBJECTIVE: To study diagnostic informational content of change of concentration a hypoxia-inducible factor (HIF-1α) in gingival liquid of patients for assessment of risk of development of carious process. MATERIAL AND METHODS: 75 patients with the diagnosis dentine caries entered the research: group 1 (n=30) involved patients with primary caries while group 2 (n=45) consisted of patients with caries recurrence in 12 months after treatment. RESULTS: In all patients HIF-1α was determined in gingival liquid before treatment by the IFA method. Logistic regression and the ROC analysis were used for the statistical analysis. In group 2 the level of HIF-1α was two-fold higher than in group 1 (p<0.001) and 82% higher than in healthy individuals (p<0.001). The increased initial HIF-1α level of 98 pg/ml was associated with high risk of caries recurrence in 12 months after treatment with diagnostic sensitivity of 88.4% and diagnostic specificity of 78.1% with chances of development increased by 4 times (p<0.0001). The change of HIF-1α in gingival liquid from 80 pg/ml to 170 pg/ml increased the risk of caries recurrence 12 months from 13% up to 94%. CONCLUSION: The initial content in gingival liquid a hypoxia-inducible factor-1α higher than 98 pg/ml allows triage of patients with high risk of caries recurrence requiring dental monitoring for personification of preventive measures.

[Diagnostic and prognostic significance of the hypoxia-dependent factor-1α for the development of a carious lesion]. / Diagnosticheskaia i prognosticheskaia znachimost' gipoksiia- zavisimogo faktora-1α dlia razvitiia karioznogo porazheniia.

The research objective was to study the diagnostic value of a hypoxia-dependent factor (HIF-1α) in gingival fluid for assessment of carious process risk. 75 patients with dentine caries entered were enrolled in the study: group 1 (n=30) - primary caries, group 2 (n=45) - secondary recurrent disease (12 months after treatment). HIF-1α was assessed in in gingival liquid of all patients before treatment by the IFA method. Statistical analysis included logistic regression and the ROC analysis. In the 2nd group of patients HIF-1α value was two-fold higher than in group 1 (p<0.001) and by 82% in healthy controls (p<0.001). The ROC analysis revealed that the initial HIF-1α level higher than 98 pg/ml was associated with high risk of caries relapse in 12 months after treatment (with diagnostic sensitivity of 88.4% and specificity of 78.1%), and chances of disease development are increased by 4 times (p<0.0001). Logistic regressions created a mathematical model for calculation of caries relapse prognosis. Pearson's χ2=50.3 (p<0.0001) indicated the statistical importance of dependence between the risk and the size of a predictor. HIF-1α concentration change in gingival liquid from 80 pg/ml to 170 pg/ml correlates with the 12 months recurrent disease risk growth from 13 up to 94%. Thus, the initial gingival fluid content of HIF-1α higher than 98 pg/ml indicates high risk for caries relapse and demands personification of preventive measures of the disease progression.