RESUMEN
The effects of temperature on population development of 11 species of stunt nematodes in the subfamily Tylenchorhynchinae were compared on red clover or Kentucky bluegrass in constant-temperature tanks at 5-degree intervals from 10 to 35 C. The optimum temperature for population increase on red clover in 90 days was 30 C for Tylenchorhynchus agri, T. nudus, T. martini, and T. clarus, 25 C for T. sylvaticus and T. dubius, and 20 C for T. canalis, Merlinius brevidens, and Quinisulcius capitatus. The optimum was 30 C for T. robustoides and 25 C for T. maximus on Kentucky bluegrass. The temperature range for population increase was 20-35 C for T. agri, T. nudus, T. martini, and T. clarus, 20-30 C for T. sylvaticus and T. robustoides, 15-25 C for T. maximus, 10-25 C for T. dubius, and 10-20 C for M. brevidens and Q. capitatus. T. canalis increased only at 20 C. All species were recovered in numbers near their inoculum level at 10 C. There was no survival of T. sylvaticus, T. dubius, T. canalis, T. robustoides, T. maximus, M. brevidens, and Q. capitatus at 35 C, or of the last three of these species at 30 C. Temperature had no effect on sex ratio in final populations. Population increase was greatest in T. martini and least in T. canalis.
RESUMEN
The effect of temperature on pine wilt development in Scots pine (Pinus sylvestris) was examined in three experiments. Container-grown pines (4-6 years old) inoculated with 1,500 Bursaphelenchus xylophilus were incubated at constant temperatures in growth chamber for 8 weeks, then at a temperature range of 15-30 C in a greenhouse for 10-12 weeks. Nematode infection was greater, tree mortality was higher, and disease incubation was shorter at 32 and 30 C than at 25, 23, 18, and 11 C. Foliar symptoms developed more rapidly and uniformly at higher temperatures. Ninety-five percent of tree deaths at 32 and 30 C and 88% at 25 and 23 C occurred within the 8-week exposure to constant temperatures. Mortality at 18, 16, and 11 C occurred only after transfer to the greenhouse. Results indicate that pine wilt incidence is directly related and disease incubation period is inversely related to temperature and that high-temperature stress predisposes Scots pine to lethal infection by B. xylophilus.
RESUMEN
Size of lesions caused by Pratylenchus scribneri on roots of 'Clark 63' soybean was correlated with nematode colony size within roots. A single nematode was capable of causing a detectable lesion. When a root became highly necrotic and shrunken, few nematodes but numerous eggs remained in the tissue. In histological sections made 5, 11, 18, and 45 d after planting, P. scribneri was located entirely within the cortex and generally was oriented longitudinally to the vascular cylinder, either outstretched in the same plane or coiled through several cells. Nematodes moved intracellularly, causing extensive rupturing of cell walls, retraction and disappearance of cytoplasm, and thickening of cell walls and necrosis of cells around feeding sites. Depth of penetration within the cortex and necrosis of cells increased with time after infection, eventually resulting in formation of cavities in the cortex and occasional secondary injury to the endodermis. Stele tissue was unaffected by feeding, and damage to the epidermis was limited to nematode entry points. Orientation of P. alleni and histopathology of its infection at 45 days were identical to those of P. scribneri, except that there was no injury to the endodermis.
RESUMEN
In a soil temperature study, population increase on 'Clark 63' soybeatt was most rapid at 30 C in Pratylenchus alleni, P. brachyurus, P. cofleae, P. neglectus, P. scribneri, and P. zeae and at 25 C in P. penetrans and P. vulnus. The last two were the only species that reproduced at 15 C. Populations of all species increased over the range of 20-30 C, except those of P. neglectus at 20 C and P. coffeae, which was not tested below 25 C. Only P. brachyurus, P. neglectus, P. scribneri and P. zeae reproduced at 35 C. At their optimum temperatures, P. scribneri exhibited the greatest population increase, 1248-fold, and P. penetrans the least, 32-fold. This is the first report of soybean as a host for P. vulnus.
RESUMEN
The effect of Tylenchorhynchus nudus on growth of Kentucky bluegrass was investigated under controlled environmental conditions in both a phytotron and a greenhouse. The nematode significantly reduced weights of clippings, crowns and roots. Pathogenicity was greater in sandy loam soil than in loam and was enhanced by submitting plants to nutrient and/or moisture stresses; soil nutrient level was most critical. The results suggest that T. nudus contributes significantly to summer decline of bluegrass lawns in South Dakota.
RESUMEN
Population development of isolates of Heterodera lespedezae from Illinois and North Carolina was compared on Korean, sericea, and striate lespedezas and red clover at soil temperatures of 14, 18, 22, 26, 30, 34 C (+/- 1 C) and in a greenhouse where temperatures ranged from 19 to 40 C (av. 25 C). Cyst production on all plants, but not at all temperatures, was significantly different between the two isolates. Males of the Illinois isolate were recovered from red clover and striate lespedeza at 22 and 26 C and at greenhouse temperatures. No males of the North Carolina isolate were found on any host. Both isolates retarded growth of striate lespedeza but had no effect on growth of the other species tested.
RESUMEN
The host preferences of populations of Meloidogyne naasi from England, California, Illinois, Kentucky and Kansas were compared. Among 22 plant species tested, most were hosts for isolates of all five populations; crabgrass was added to the list of known hosts. Differential reactions of isolates on creeping bentgrass, curly dock, sorghum, and common chickweed demonstrated the existence of at least five physiological races within M. naasi. The known races are numerically designated and characterized.
RESUMEN
The developmental rates of the five newly designated races of Meloidogyne naasi were compared on barley, oat and sorghum. Races 1, 2, 3 and 4 developed and reproduced on both barley and oat but not on sorghum. Race 5 developed and reproduced readily on sorghum but poorly on oat. A more rapid rate of development of Race 5 on both barley and sorghum than that of other races on barley demonstrated that Race 5 has a shorter life cycle than do Races 1-4.
RESUMEN
Reproduction and pathogenicity of the five known races of Meloidogyne naasi on two selections of creeping bentgrass were compared. Toronto C-15 was a host for Races 3, 4 and 5, whereas Northmoor 9 supported reproduction of all five races. Differences in susceptibility and population increase demonstrated that the races could be separated by degree of reproduction on the two selections. Root weights generally were unaffected. Based on cumulative dipping weights, all but Race 1 were pathogenic on at least one of the selections; Race 3 stunted top growth of both. Slight differences in degree of pathogenicity, associated with final populations, were not broad enough to be useful in race separation.
RESUMEN
The pathogenicity and interactions of Meloidogyne naasi, Pratylenchus penetrans, and Tylenchorhynchus agri on 'Toronto C-15' creeping bentgrass, Agrostis palustris, was studied in a long-term greenhouse experiment. Based on dry weights of roots and clippings, M. naasi alone and in all combinations with P. penetrans and T. agri was highly pathogenic to creeping bentgrass. P. penetrans and T. agri alone and in combination inhibited root growth but adversely affected top growth only when the two were co-inoculated. In combination, the effects of each species on top growth were additive, with M. naasi the dominant pathogen. Creeping bentgrass was an excellent host for M. naasi and T. agri, but a poor host for P. penetrans. T. agri inhibited population increase of M. naasi, indicating nematode-nematode competition, but neither T. agr/ nor P. penetrans was affected by any of the combinations.