First epidemiological data from the French Trophoblastic Disease Reference Center.

OBJECTIVE: The objective of the study was to describe women registered at the new French Trophoblastic Disease Reference Center and particularly the rates of gestational trophoblastic neoplasia (GTN) after molar pregnancies. STUDY DESIGN: Epidemiological data from a prospective cohort of women registered between November 1999 and November 2004 were analyzed. RESULTS: Four hundred forty-eight women were registered. The referent pathologist reclassified 32% and 5% of assumed partial mole (PM) and complete mole (CM), respectively. GTN developed in 30 of 212 patients with singleton CM (14%) and in 5 of 108 with singleton PM (5%). Among 131 patients with GTN (35 women followed up after registration for a mole and 96 registered for a GTN), 115 (88%) were low-risk and 16 (12%) were high-risk patients according to 2000 International Federation of Gynecology and Obstetrics (FIGO) scoring system. CONCLUSION: Creation of trophoblastic disease reference centers is desirable to improve treatment of patients. Our results will have to be compared with future publications based on the new 2000 FIGO oncology committee recommendations.

Glucocorticoid-induced maturation of glycoprotein galactosylation and fucosylation processes in the rat small intestine.

We determined the role of glucocorticoids in the maturation of glycoprotein galactosylation and fucosylation processes in the rat small intestine during postnatal development. Treatment of suckling rats with hydrocortisone (HC) increased activities of an O-glycan: galactosyltransferase, and of an alpha-1,2-fucosyltransferase, through transcriptional regulation of the FTB gene. The activities of a fucosyltransferase inhibitor and of the enzymes responsible for the synthesis and degradation of GDP-fucose were unaffected by the treatment, whereas a fall in the activity of alpha-L-fucosidase was observed. These changes were accompanied by the precocious appearance of alpha-1,2-fucose residues in complex glycan chains of brush-border membrane glycoproteins that normally appear after weaning, and with a trend to increase in alpha-1,2-fucose residues in mucins. Thus, treatment of suckling rats with hydrocortisone speeds up the maturation of glycoprotein galactosylation and fucosylation processes in the small intestine. The delayed increase in glucocorticoid levels induced by prolonged nursing, or the suppression of glucocorticoids by adrenalectomy (AD) before the normal rise in the hormone, both induced a delay in the increases in activities of the O-glycan: galactosyltransferase and alpha-1,2-fucosyltransferase observed normally after glucocorticoid enhancement. Thus, glucocorticoids might play at least a partial role in the maturation of glycoprotein glycosylation observed at weaning.

Anabolic and catabolic hormonal responses to experimental two-set low-volume resistance exercise in sedentary and active elderly people.

BACKGROUND AND AIMS: The influence of acute low-volume resistance exercise on serum growth hormone (GH), insulin-like growth factor I (IGF-I), dehydroepiandrosterone sulphate (DHEAS), total testosterone (TT) and cortisol was ascertained in elderly subjects. METHODS: Forty-seven independent, community-dwelling volunteers aged >65 years were recruited: 23 (11 men, 12 women) were sedentary individuals, and 24 (12 men, 12 women) had been regularly involved in physical activity for several years. The protocol consisted of two sets of leg extensions: one graded by loading to reach maximal power; the other consisted of 10 consecutive leg extensions using a load corresponding to maximal power. RESULTS: IGF-I levels increased immediately after exercise, returning almost completely to pre-exercise values by the 15-minute post-exercise time point. The changes in all four study groups were similar. Not any of the groups presented systematic exercise-induced changes in circulating GH, DHEAS and TT levels. With respect to pre-exercise cortisol levels, significant decreases were observed both at the immediate and at the 15-minute post-exercise time points. These changes were independent of gender and physical activity level. CONCLUSIONS: Our data indicate that low-volume resistance exercise may decrease cortisol levels and increase serum anabolic/catabolic hormone ratios. In view of the experimental character of our resistance training protocol and the lack of control-day data, these results should be corroborated by long-term low-volume resistance training programs.

Testosterone measured by 10 immunoassays and by isotope-dilution gas chromatography-mass spectrometry in sera from 116 men, women, and children.

BACKGROUND: Commercially available testosterone immunoassays give divergent results, especially at the low concentrations seen in women. We compared immunoassays and a nonimmunochemical method that could quantify low testosterone concentrations. METHODS: We measured serum testosterone in 50 men, 55 women, and 11 children with use of eight nonisotopic immunoassays, two isotopic immunoassays, and isotope-dilution gas chromatography-mass spectrometry (ID/GC-MS). RESULTS: Compared with ID/GC-MS, 7 of the 10 immunoassays tested overestimated testosterone concentrations in samples from women; mean immunoassay results were 46% above those obtained by ID/GC-MS. The immunoassays underestimated testosterone concentrations in samples from men, giving mean results 12% below those obtained by ID/GC-MS. In women, at concentrations of 0.6-7.2 nmol/L, 3 of the 10 immunoassays gave positive mean differences >2.0 nmol/L (range, -0.7 to 3.3 nmol/L) compared with ID/GC-MS; in men at concentrations of 8.2-58 nmol/L, 3 of the 10 immunoassays tested gave mean differences >4.0 nmol/L (range, -4.8 to 2.6 nmol/L). CONCLUSION: None of the immunoassays tested was sufficiently reliable for the investigation of sera from children and women, in whom very low (0.17 nmol/L) and low (<1.7 nmol/L) testosterone concentrations are expected.