RESUMEN
Mice exposed to a chronic auditory stressor and treated with nefazodone (10 mg/kg/day s.c.), showed a reduction in stress-induced suppression of thymus and spleen cellularity, and in peripheral T-Iymphocyte population. The in vitro blastogenic response of spleen lymphoid cells to mitogen concanavalin A, the in vitro and in vivo activity of phagocytosis, both measured using the zymosan and carbon clearance tests, respectively, were also assessed and nefazodone was found to partially reverse the inhibitory effect of stress on those parameters. Nefazodone did not significantly affect those parameters in unstressed mice. In conclusion, this report provides evidence on the immunoprotective effects of this novel antidepressant drug against the adverse effects of stress in mice.
Asunto(s)
Inmunidad/efectos de los fármacos , Linfocitos T/efectos de los fármacos , Triazoles/farmacología , Animales , Antidepresivos/farmacología , Concanavalina A/farmacología , Femenino , Ratones , Ratones Endogámicos BALB C , Norepinefrina/metabolismo , Fagocitosis/efectos de los fármacos , Piperazinas , Serotonina/metabolismo , Antagonistas de la Serotonina/farmacología , Bazo/patología , Estrés Fisiológico/inmunología , Estrés Fisiológico/patología , Linfocitos T/inmunología , Timo/patologíaRESUMEN
Experiments were conducted to evaluate the effects of amphetamine (0. 4 mg/kg) on the development of autochthonous tumors induced by the Moloney sarcoma virus (MSV) in Balb/c female mice. Enhancement of MSV-induced tumor incidence and tumor growth was observed, together with a delay in the usual prompt regression of the tumors, when mice were daily injected with amphetamine for 3 days after MSV-inoculation. However, no effects of amphetamine on tumor development were observed when it was administered during the 3 days before tumor inoculation.
Asunto(s)
Adrenérgicos/farmacología , Anfetamina/farmacología , Virus del Sarcoma Murino de Moloney , Infecciones por Retroviridae/patología , Sarcoma Experimental/patología , Infecciones Tumorales por Virus/patología , Animales , Femenino , Inmunidad Celular/efectos de los fármacos , Inyecciones Subcutáneas , Ratones , Ratones Endogámicos BALB C , Premedicación , Infecciones por Retroviridae/tratamiento farmacológico , Sarcoma Experimental/tratamiento farmacológico , Infecciones Tumorales por Virus/tratamiento farmacológicoRESUMEN
Mice exposed to a chronic auditory stressor and treated with fluoxetine (5 mg/kg) showed a reduction in stress-induced suppression of thymus and spleen cellularity, and in peripheral T lymphocyte population. The blastogenic response of spleen lymphoid cells and the delayed type hypersensitivity response (DTH) to sheep red blood cells (SRBC) were also assessed and fluoxetine was found to partially reverse the inhibitory effect of stress on both parameters.
Asunto(s)
Recuento de Células/efectos de los fármacos , Fluoxetina/farmacología , Terapia de Inmunosupresión , Estrés Fisiológico/tratamiento farmacológico , Linfocitos T/efectos de los fármacos , Animales , Femenino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Bazo/efectos de los fármacos , Timo/efectos de los fármacosRESUMEN
Late-onset drinking is a common problem in elderly people related to stress induced by social isolation. Experiments were performed in order to evaluate the effects of alprazolam, a benzodiazepine agonist anxiolytic, on the free-choice ethanol consumption in aged rats subjected to isolation stress. The animals we offered a two-bottle choice consumption (one of 0.2% saccharin and the other with 10% ethanol/0.2% saccharin) and then exposed to 4 days of isolation stress on an irregular, unpredictable schedule. Stress resulted in significant increase in ethanol consumption. Treatment with alprazolam (1 mg/Kg) partially reversed this adverse effect of stress.
Asunto(s)
Envejecimiento , Consumo de Bebidas Alcohólicas/tratamiento farmacológico , Alcoholismo/tratamiento farmacológico , Alprazolam/farmacología , Ansiolíticos/farmacología , Aislamiento Social , Estrés Psicológico/complicaciones , Consumo de Bebidas Alcohólicas/psicología , Alcoholismo/etiología , Alprazolam/uso terapéutico , Animales , Ansiolíticos/uso terapéutico , Modelos Animales de Enfermedad , Etanol/metabolismo , Femenino , Ratas , Ratas Sprague-DawleyRESUMEN
Experiments were performed in order to evaluate the effects of fluoxetine, a selective inhibitor of neural serotonin transporter antidepressant, on the development lung metastases in rats subjected to laparotomy and injected (i.v.) with 10(4) Walker 256 (W-256) carcinosarcoma cells. The number of metastatic nodules on the surface of the lungs, as well as the percentage-area of metastases in the frontal section through pulmonary hilus were increased in rats subjected to sham-surgery or laparotomy. Treatment with fluoxetine (5 mg/kg) partially reversed those adverse effects of surgery, but the difference was clearer when it was administered before surgery was performed. Survival periods were also assessed and fluoxetine was found to decrease the lethality of rats exposed to surgery.
Asunto(s)
Antidepresivos de Segunda Generación/farmacología , Antineoplásicos/farmacología , Carcinoma 256 de Walker/tratamiento farmacológico , Carcinoma 256 de Walker/secundario , Fluoxetina/farmacología , Neoplasias Pulmonares/tratamiento farmacológico , Neoplasias Pulmonares/secundario , Inhibidores Selectivos de la Recaptación de Serotonina/farmacología , Estrés Fisiológico/complicaciones , Procedimientos Quirúrgicos Operativos/efectos adversos , Animales , Masculino , Trasplante de Neoplasias , Ratas , Ratas Sprague-Dawley , Estrés Fisiológico/etiologíaRESUMEN
Experiments were conducted to evaluate the effects of amphetamine (0. 4 mg/kg of body weight/day) on the development of oral candidiasis in Sprague-Dawley rats. Animals were submitted to surgical hyposalivation in order to facilitate the establishment and persistence of Candida albicans infection. Treatment with drugs (placebo or amphetamine) was initiated 7 days before C. albicans inoculation and lasted until the end of the experiments, day 15 postinoculation. Establishment of C. albicans infection was evaluated by swabbing the inoculated oral cavity with a sterile cotton applicator on days 2 and 15 after inoculation, followed by plating on YEPD (yeast extract-peptone-dextrose) agar. Tissue injury was determined by the quantification of the number and type (normal or abnormal) of papillae on the dorsal tongue per microscopic field. A semiquantitative scale was devised to assess the degree of colonization of the epithelium by fungal hyphae. Our results show that amphetamine exacerbates C. albicans infection of the tongues of rats. Significant increases in Candida counts, the percentage of the tongue's surface covered with clinical lesions, the percentage of abnormal papillae, and the colonization of the epithelium by fungal hyphae were found in amphetamine-treated rats compared to those found in the rats injected with a placebo. The last two parameters increased in rats treated with the placebo compared to the parameters of the untreated control rats.