Antibacterial effect, cytotoxicity, and bond strength of a modified dental adhesive containing silver nanoparticles.

This study aimed to evaluate the antibacterial effect, cytotoxicity, and microtensile bond strength of an adhesive system containing silver nanoparticles (NAg). NAg was synthesized and incorporated (500 and 1000 ppm) into Scotchbond Multi-Purpose (SBMP) primer and bond. A microtensile bond test (µTBS) was performed after 24 h and 1 year. The adhesive interface was characterized using a confocal Raman microscope. The antibacterial activity was assessed using agar diffusion and biofilm inhibition assays (S. mutans). MTT assay was used to assess the cytotoxicity of NAg-conditioned culture media on human dental pulp stem cells (hDPSCs). The results were statistically analyzed using analysis of variance and Tukey's tests (α = .01). Incorporating 500 and 1000 ppm of NAg in the SBMP did not affect the µTBS after 24 h (p > 0.05). However, in the 1 year evaluation, 500 ppm presented the highest µTBS values (p < 0.05). The addition of NAg at 500 and 1000 ppm in the primer and bond led to larger inhibition halos and colony-forming units than the control (p < 0.05). For the unpolymerized and polymerized groups, the combination of primer and bond presented the highest cytotoxic effects on hDPSCs (p < 0.05). In conclusion, incorporating 500 or 1000 ppm of NAg into an etch-and-rinse adhesive system led to an antibacterial effect without altering the cytotoxicity. SBMP at 500 ppm presented a higher µTBS at 1 year.

Optical parameters and hardness of two maxillofacial elastomers after immersion in different solutions of Brazilian green propolis extract.

STATEMENT OF PROBLEM: Maxillofacial elastomers undergo physical and mechanical degradation with disinfecting solutions. Solutions of Brazilian green propolis extract may be suitable alternatives for infection control of maxillofacial prostheses. However, their effects on the properties of the material are unknown. PURPOSE: The purpose of this in vitro study was to evaluate the effect of disinfection with solutions of Brazilian green propolis extract on the transmittance, translucency parameter, contrast ratio, and hardness of 2 maxillofacial elastomers (MDX4-4210 and MED-4014). MATERIAL AND METHODS: Fifty disk-shaped specimens (3×10 mm) of each elastomer were randomly and equally divided into 4 groups of disinfectant agents and 1 control group: 3 separate groups of 11% green propolis extracts including aqueous (PAQ), glycolic (PGL), and alcoholic (PAL), a 2% chlorhexidine gluconate (CHX) group, and the control group of distilled water. Specimens were subjected to disinfection by immersion 3 times a week for 60 days. Color differences (ΔE values) were calculated with CIELab and CIEDE2000 formulas. Optical parameters and Shore A hardness were determined at 2 time points: at baseline and after the period of specimen disinfection. Data were analyzed by parametric and nonparametric analysis of variance and by multiple-comparison tests (α=.05). RESULTS: The ΔE values of specimens immersed in 11% PAL were not clinically acceptable for either elastomer. Regarding translucency parameter and contrast ratio, the immersion in 11% PAL and 11% PGL resulted in greater opacity and lower translucency of the material. Mean Shore A hardness values were not statistically significantly different at baseline or after 60 days of immersion in the solutions. CONCLUSIONS: The solution of Brazilian green propolis extract tested showed changes in optical parameters. Elastomers immersed in 11% alcoholic green propolis extract showed clinically unacceptable color and translucency changes. All hardness values of the tested elastomers were clinically acceptable after immersion in all tested disinfectant groups.

Relationship between toothpastes properties and patient-reported discomfort: crossover study.

OBJECTIVES: This study aims to correlate patient-reported reactions with in vitro analyses of the pH, abrasive quality, and cytotoxicity of four toothpastes. MATERIALS AND METHODS: One hundred twenty-one patients received non-identified samples of toothpaste to be used for 6 days and answered a questionnaire about their sensations. In vitro analysis: the pH of toothpastes was measured with a pH meter. The abrasivity of toothpastes was evaluated against composite resin specimens (n = 10). A toothbrushing machine was used to simulate wear, which was indirectly measured by mass loss using a scale. Cell culture media conditioned with toothpaste were used to assess the cytotoxicity. Confluent cells were kept in contact with the conditioned media or control for 24 h. The cell viability was measured using the 3-(bromide, 4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium (MTT)-reduction assay. The obtained data on the pH, weight loss, and cell viability were compared by ANOVA/Tukey's tests (p < 0.05). RESULTS: With the exception of the bleaching effect paste, the Oral B® paste produced the highest frequencies of irritation reports, tooth sensitivity, taste discomfort, and texture discomfort in the clinical study; patients also reported rougher teeth, soft tissue peeling, dry mouth, thrush, tingling, and taste changes in response to this paste. The in vitro analysis demonstrated that Oral B® had the lowest pH, the highest abrasivity, and produced the lowest cell viability (p < 0.01). CONCLUSION: Results suggest that low pH toothpastes that are highly abrasive and cytotoxic may cause undesirable reactions in patients. CLINICAL RELEVANCE: Toothpaste's properties should be well known for indication to patient therefore minimizing discomfort reports.

Surface treatment of dental porcelain: CO2 laser as an alternative to oven glaze.

This work tested continuous CO2 laser as a surface treatment to dental porcelain and compared it to oven glaze (auto-glaze) by means of roughness and color parameters. Three commercial veneering porcelains with different crystalline content were tested: VM7, VM9, and VM13. Porcelain discs (3.5 × 2.0 mm, diameter × height) were sintered and had one side ground by a diamond bur (45 µm) simulating a chairside adjustment in a clinical office. Specimens (n = 7) were divided into the following groups: C--control (no treatment), G--auto-glaze (oven), and L--surface continuous irradiation with CO2 laser (Gem Laser, Coherent; λ = 10.6 µm). Laser was tested in three exposure times (3, 4, or 5 min) and two irradiances (45 and 50 W/cm(2)). Roughness parameters (Ra, Rz, and Rpm/Rz) were measured using a rugosimeter (Surftest 301, Mitutoyo). Color differences (ΔE) between the G and L groups were calculated (VITA Easyshade); ΔE values up to 3.3 were considered as not perceivable. A surface analysis was conducted by stereomicroscopy (Olympus SZ61) and SEM (Stereoscan 440, LEO). Crystalline content of specimens from groups C and L (50 W/cm(2), 5 min) was assessed by X-ray diffraction and then compared. Surface roughness (Ra and Rz) observed for laser-irradiated groups was similar to G for all studied porcelains. Rpm/Rz ratios were near 1.0 for all groups that indicated a sharp ridge profile for all specimens. Only one laser condition studied (50 W/cm(2), 3 min) from VM7 porcelain resulted in color difference (ΔE = 3.5) to G. Specimens irradiated with 50 W/cm(2) for 5 min presented the smoother surface observed by SEM, comparable to G. X-ray diffraction data revealed an increase in leucite crystallite size for VM9 and VM13 porcelains after laser treatment. Regarding roughness, continuous CO2 laser applied on porcelain surface was as effective as conventional oven auto-glaze.

Comparison of torque expression among passive self-ligating brackets with different slot depths: An in vitro study.

INTRODUCTION: The aim of this study was to assess the interaction between a 0.019×0.025-inch (″) stainless steel archwire and two types of passive self-ligating brackets with the same slot height (0.022″) and different slot depths (0.028″ and 0.026″, and to measure the archwire/slot play as well as to compare the torque expression with archwire torsions of 12°, 24°, and 36°. MATERIAL AND METHODS: An experimental device was developed along with a universal testing machine to measure torque expression in two types of brackets with 0.028″ and 0.026″ slot depths. Analysis of variance (ANOVA) and Tukey's test were performed to identify the differences between groups. RESULTS: The 0.026″ slot bracket presented greater archwire/slot play when compared to the 0.028″ bracket. Torque expression with torsions of 24° and 36° were significantly higher in the 0.028″ depth brackets when compared to the 0.026″ depth brackets. CONCLUSION: The 0.022″×0.026″ passive self-ligating brackets attached with a 0.019″×0.025″ stainless steel archwire provided no greater torque control when compared to 0.022″×0.028″ passive self-ligating brackets.

Post-cure heat treatments influence on mechanical and optical properties of resin composites.

This study evaluated the effect of post-cure heat treatment (PCHT) on the Knoop microhardness (KHN), degree of conversion (DC), color changes, and contrast ratio (CR) of four resin composites (RCs): Z100 (3M ESPE), Z350 XT (3M ESPE), Estelite Omega (Tokuyama) and Empress Direct (Ivoclar Vivadent). Specimens (12 × 1 mm) were prepared for each material (n = 10 / group). After curing, samples were subjected to PCHT for 10 min at 100°C or 170°C. Control group was maintained at room temperature (24°C) for the same time. The DC was analyzed by FT-NIR immediately and 24 h after the PCHT (n = 3 / group). KHN was analyzed 24 h after PCHT (n = 10 / group). According to CIEDE2000 (∆E00), color measurements were obtained immediately after curing, five minutes after PCHT, and after seven days of storage in water, coffee, and red wine. Data were analyzed by One and Two-Way ANOVA (p < 0.05). Z100, Z350, and Estelite Omega showed increases in KHN with increased temperature (p < 0.05). PCHT at 100°C and 170°C led to a higher DC of all RCs (p < 0.05). Initially, the PCHT lead to increased ∆E00 values (p < 0.05), which was decreased after immersion in coffee and wine (p < 0.05). Considering the effect of PCHT and staining solutions, lower color changes were observed in the thermally treated specimens (p < 0.05). Taken collectively, the results suggest the PCHT as an economical and practical alternative to enhance direct RC's properties in direct-indirect and indirect restorations.

Changes in color and contrast ratio of resin composites after curing and storage in water.

PURPOSE: To verify the color change and contrast ratio of resin composites after curing and after 30 days of storage in water. METHODS: Dentin A2 shades of different light-cured dental resin composites (Vittra APS, FGM, Brazil; Z350 XT, 3M ESPE, EUA; Tetric N-Ceram, Ivoclar Vivadent, Liechtenstein, and Charisma Diamond, Heraeus Kulzer, Germany) were tested. Ten rounded specimens (8 mm × 2 mm) were prepared for each material. Reflectance for all samples was obtained using a spectrophotometer (Minolta CM 3700d, Konica Minolta, Japan) before curing, immediately after curing, and after 30 days of storage in water. The color change (ΔE*lab) and contrast ratio (CR) data were analyzed using a one-way analysis of variance with Tukey's and paired t-tests (α = 1%). RESULTS: For all materials tested, significant color changes were noticeable after curing and after 30 days in water (p < 0.01). Significant changes in the CR values before curing, after curing, and 30 days of storage in water were observed in the resin composites investigated (p < 0.01) except for Z350 (p > 0.01). CONCLUSION: The CR values and color changes after curing and 30 days of storage in water varied depending on the material tested. This study corroborates the clinical practice of curing a small amount of unpolymerized resin composite on the tooth surface to select the desired shade before undertaking esthetic restorative procedures.

Is prolonged bleaching more harmful to dental enamel than daily dietary and hygienic oral habits?

The ultrastructural and mechanical properties of enamel surface were evaluated after prolonged bleaching treatments with 10% carbamide peroxide in the presence or absence of orange juice (erosive challenge) and toothbrushing (abrasive challenge). In total, 145 incisor bovine teeth were used in this study. Twenty-five samples were prepared for the ultrastructural evaluations, and 120 samples were prepared for microhardness and roughness tests. These 120 samples were divided into eight experimental groups (n = 15): G1- artificial saliva; G2- abrasion; G3- erosion; G4- dental bleaching; G5- erosion + abrasion; G6- bleaching + abrasion; G7- bleaching + erosion; and G8- bleaching + erosion + abrasion. All groups were tested at T0 (before treatment), T1 (14 days), T2 (21 days), and T3 (28 days). Two-way analysis of variance for repeated measures and the post hoc Sidak tests (p ≤ 0.05) were used. The roughness evaluation demonstrated an increase in damage for all experimental groups with an increase in the time period. For microhardness, the groups exposed to artificial saliva (AS) and abrasive challenge did not show any differences at any time points, while the other groups showed a decrease in microhardness from T0 to T3. Ultrastructural evaluation showed different surface alterations in response to the treatments. Despite prolonged bleaching periods, the procedure caused lesser enamel surface alterations than exposure to orange juice alone or in combination with brushing.

Effect of light irradiation on tooth whitening: enamel microhardness and color change.

The aim of this study was to evaluate the influence of light exposure associated with 35% hydrogen peroxide (Pola Office, SDI, Melbourne, Vic., Australia) or 15% hydrogen peroxide (BriteSmile, Discus, Culver City, CA, USA) on the microhardness and color changes of bovine enamel. Experimental groups were Britesmile + Light (BL) (15% hydrogen peroxide + plasm arc; 4 x 20 minutes), Britesmile + No Light (BN) (BL, no light), Pola office + Light (PL) (35% hydrogen peroxide + LED; 4 x 8 minutes), and Pola office + No light (PN) (PL, no light). Color changes (DeltaE) and the CIELAB (Commission Internationale de l' Eclairage, L* a* b* color system) parameters (L*, a*, and b*) were assessed with a spectrophotometer before (B), immediately (A), 1 day and 7 days after bleaching. The microhardness was measured before (B) and after (A), the obtained data were submitted to a two-way analysis of variance, and DeltaE were submitted to t-test for each period. Only Pola Office, in which the peroxide is associated with the light, improved DeltaE when evaluated immediately after bleaching (p < 0.001). Light exposure did not influence DeltaE after 1 day or 7 days for either bleaching system. The enamel microhardness was not altered after bleaching for BriteSmile. However, enamel microhardness was reduced after bleaching for Pola Office, 283 MPa (+/-21) and 265 MPa (+/-27), respectively. It was concluded that these two bleaching systems were efficient regardless of the light systems used. However, the 35% hydrogen peroxide altered the enamel microhardness. CLINICAL SIGNIFICANCE Enamel microhardness was affected by a 35% hydrogen peroxide in-office bleaching therapy. Moreover, the in-office bleaching outcome was not improved by using the light associated with systems tested in this study. (J Esthet Restor Dent 21:387-396, 2009).

Composite depth of cure obtained with QTH and LED units assessed by microhardness and micro-Raman spectroscopy.

This study analyzed the depth of cure of a composite assessed by microhardness and the degree of conversion as a function of the light cure unit (LCU) used. Two light cure units, one LED (Ultraled-Dabi Atlante) and one quartz-tungsten-halogen (QTH, Optilux 401-Demetron) unit were used to cure 4.0 x 4.0 mm and 5.0 mm deep composite specimens (Filtek Z250, 3M ESPE). After 24 hours storage at 37 degrees C, Knoop microhardness and degree of conversion were measured on the irradiated surface and at each millimeter of the sample's depth. The degree of conversion was determined by using micro-Raman spectroscopy. The specimens cured with the QTH unit presented uniform decay in microhardness up to 4 mm in depth. Beyond 4 mm, the drop was abrupt. With LED photoactivation, uniform decay was observed only up to 2 mm. At higher depths, the decay in microhardness increased rapidly, especially beyond 3 mm. Depth of cure assessed by micro-Raman revealed that the degree of conversion behaved similarly to microhardness for both LCUs. A strong linear regression between microhardness and the degree of conversion, including both LCUs, was established with R2 = 0.980.

Influence of the Extended Use of Desensitizing Toothpastes on Dentin Bonding, Microhardness and Roughness.

The aim of this study was to evaluate the influence of an extended use of desensitizing toothpastes (DTs) on dentin bonding, microhardness and roughness. One hundred and twenty bovine incisor teeth were randomly divided into four groups: G1, distilled water (WATER); G2, Colgate Total 12 (CT12); G3, Colgate Sensitive Pro-Relief (CSPR); and G4, Sensodyne Repair ?αµπ; Protect (SRP). Dentin surfaces were etched with 17% EDTA and 2 years of simulated tooth brushing (20,000 cycles) was performed on their surfaces. Knoop microhardness, surface roughness and scanning electron microscopy (SEM) were performed before and after the simulated tooth brushing. For microshear bonding test, a 2-step self-etching adhesive system (Clearfil SE Bond) was used and 0.8 mm diameter composite resin (Filtek Z350 XT) cylinders were built. Microshear test was performed with an orthodontic wire and with a crosshead speed of 0.5 mm/min. The data were analyzed for: 1) bond strength (one-way ANOVA), 2) microhardness intra-group (Student's test) and inter-group (one-way ANOVA/Tukey's test) comparisons, 3) roughness intra-group (Student's test/Wilcoxon's test) and inter-group (Kruskal-Wallis/Student-Newman-Keuls test) comparisons. The extended use of both dentifrices (conventional and for sensitive teeth) did not affect the bond strength and produced a significant increase in microhardness and roughness of the dentin, except for the microhardness of the SRP group. The simulated tooth brushing technique with water produced an increase in roughness, without reducing significantly the dentin microhardness.

Post-cure heat treatments influence on mechanical and optical properties of resin composites

Abstract This study evaluated the effect of post-cure heat treatment (PCHT) on the Knoop microhardness (KHN), degree of conversion (DC), color changes, and contrast ratio (CR) of four resin composites (RCs): Z100 (3M ESPE), Z350 XT (3M ESPE), Estelite Omega (Tokuyama) and Empress Direct (Ivoclar Vivadent). Specimens (12 × 1 mm) were prepared for each material (n = 10 / group). After curing, samples were subjected to PCHT for 10 min at 100°C or 170°C. Control group was maintained at room temperature (24°C) for the same time. The DC was analyzed by FT-NIR immediately and 24 h after the PCHT (n = 3 / group). KHN was analyzed 24 h after PCHT (n = 10 / group). According to CIEDE2000 (∆E00), color measurements were obtained immediately after curing, five minutes after PCHT, and after seven days of storage in water, coffee, and red wine. Data were analyzed by One and Two-Way ANOVA (p < 0.05). Z100, Z350, and Estelite Omega showed increases in KHN with increased temperature (p < 0.05). PCHT at 100°C and 170°C led to a higher DC of all RCs (p < 0.05). Initially, the PCHT lead to increased ∆E00 values (p < 0.05), which was decreased after immersion in coffee and wine (p < 0.05). Considering the effect of PCHT and staining solutions, lower color changes were observed in the thermally treated specimens (p < 0.05). Taken collectively, the results suggest the PCHT as an economical and practical alternative to enhance direct RC's properties in direct-indirect and indirect restorations.

Resumo Este estudo avaliou o efeito do tratamento térmico pós-cura (PCHT) na microdureza Knoop (KHN), grau de conversão (DC), mudanças de cor e razão de contraste (CR) de quatro compósitos resinosos (RCs): Z100 (3M ESPE ), Z350 XT (3M ESPE), Estelite Omega (Tokuyama) e Empress Direct (Ivoclar Vivadent). Corpos de prova (12 × 1 mm) foram preparadas para cada material (n = 10 / grupo). Após a cura, as amostras foram submetidas ao PCHT por 10 min a 100 ou 170° C. O grupo controle foi mantido à temperatura ambiente (24° C) pelo mesmo tempo. O DC foi analisada por FT-NIR imediatamente e 24 h após a PCHT (n = 3 / grupo). KHN foi analisado 24 h após PCHT (n = 10 / grupo). De acordo com o CIEDE2000 (∆E00), as medidas de cor foram obtidas imediatamente após a cura, cinco minutos após a PCHT e após sete dias de armazenamento em água, café e vinho tinto. Os dados foram analisados por ANOVA de um e dois fatores (P < 0,05). Z100, Z350 XT e Estelite Omega mostraram aumentos no KHN com o aumento da temperatura (P < 0,05). PCHT a 100 ° C e 170 ° C levou a uma maior DC de todos os RCs (P < 0,05). Inicialmente, o PCHT levou ao aumento dos valores de ∆E00 (P < 0,05), que diminuiu após a imersão em café e vinho (P < 0,05). Considerando o efeito de PCHT e soluções de coloração, menores mudanças de cor foram observadas nas amostras tratadas termicamente (P < 0,05). Os resultados sugerem o PCHT como uma alternativa econômica e prática para aumentar as propriedades diretas de compósitos resinosos em restaurações diretas-indiretas e indiretas.

Effect of Glaze Cooling Rate on Mechanical Properties of Conventional and Pressed Porcelain on Zirconia.

The aim of this study was to characterize a conventional and a pressed porcelain for zirconia core as to biaxial flexural strength (BFS), apparent fracture toughness (FT) and microstructure composition, and to investigate the effect of glaze cooling rate on the BFS of the zirconia/porcelain bilayers. Monolayers of conventional porcelain Vita VM9 and pressed porcelain Vita PM9 (n=15) (12 mm diameter x 1.2 mm thick) were prepared for the BFS test (MPa). Apparent fracture toughness (MPa.m1/2) was measured by indentation technique (n=15). t-Student test was performed for statistical analysis. Scanning electron microscopy and x-ray diffraction were used to analyze the porcelain's microstructure. For the BFS of bilayers, zirconia discs (12 mm diameter x 1 mm thick) (Vita In-Ceram YZ) were veneered with the two porcelains (1 mm thick). After the glaze firing simulation, the specimens were submitted to fast or slow cooling (n=15). Apparent fracture toughness (MPa.m1/2) was measured on the porcelain surface of bilayers (n=15) and residual stress was calculated. Two-way ANOVA (porcelain and cooling method) was used for the bilayer analysis (a=0.05). Vita PM9 monolayer exhibited significantly higher BFS (p<0.01), but there was no significant difference (p=0.41) in the FT between the porcelains. For bilayer specimens, the two-way ANOVA for BFS was significant for the porcelain variable only (p<0.01) better for Vita PM9/zirconia. Two-way ANOVA for the FT for the bilayers was not significant for any variable. All groups showed compressive residual stresses. The pressed porcelain seems to be mechanically more effective for zirconia veneering.

Is prolonged bleaching more harmful to dental enamel than daily dietary and hygienic oral habits?

Abstract The ultrastructural and mechanical properties of enamel surface were evaluated after prolonged bleaching treatments with 10% carbamide peroxide in the presence or absence of orange juice (erosive challenge) and toothbrushing (abrasive challenge). In total, 145 incisor bovine teeth were used in this study. Twenty-five samples were prepared for the ultrastructural evaluations, and 120 samples were prepared for microhardness and roughness tests. These 120 samples were divided into eight experimental groups (n = 15): G1- artificial saliva; G2- abrasion; G3- erosion; G4- dental bleaching; G5- erosion + abrasion; G6- bleaching + abrasion; G7- bleaching + erosion; and G8- bleaching + erosion + abrasion. All groups were tested at T0 (before treatment), T1 (14 days), T2 (21 days), and T3 (28 days). Two-way analysis of variance for repeated measures and the post hoc Sidak tests (p ≤ 0.05) were used. The roughness evaluation demonstrated an increase in damage for all experimental groups with an increase in the time period. For microhardness, the groups exposed to artificial saliva (AS) and abrasive challenge did not show any differences at any time points, while the other groups showed a decrease in microhardness from T0 to T3. Ultrastructural evaluation showed different surface alterations in response to the treatments. Despite prolonged bleaching periods, the procedure caused lesser enamel surface alterations than exposure to orange juice alone or in combination with brushing.

Effect of lingual enamel sandblasting with aluminum oxide of different particle sizes in combination with phosphoric acid etching on indirect bonding of lingual brackets.

OBJECTIVE: To compare bond strength and bond failure location of lingual brackets indirectly bonded after lingual enamel sandblasting with 27-, 50-, and 90-µm aluminum oxide particles followed by 37% phosphoric acid etching. MATERIAL AND METHODS: Eighty maxillary premolars were randomly divided into four equal groups according to the method of lingual enamel surface conditioning: Group 1 (control) was etched with 37% phosphoric acid, and group 2, group 3, and group 4 were sandblasted with 27-, 50-, and 90-µm aluminum oxide particles, respectively, prior to acid etching. Lingual brackets were indirectly bonded using the same protocol and adhesive (Sondhi) in all groups. The maximum shear bond strength required to debond the brackets was measured using a testing machine, and the bond failure location was classified according to the adhesive remnant index (ARI). Analysis of variance was used to compare the mean bond strength between groups. The differences between ARI scores were evaluated using the Kruskal-Wallis test. RESULTS: There were no statistically significant differences in mean shear bond strength or ARI scores between the four enamel-conditioning procedures. CONCLUSION: Lingual enamel sandblasting using different particle sizes of aluminum oxide prior to phosphoric acid etching did not increase the shear bond strength of indirectly bonded brackets and did not affect the amount of adhesive remnant on the enamel.

Effect of adhesion boosters on indirect bracket bonding.

OBJECTIVE: To determine the influence of two adhesion boosters on shear bond strength and on the bond failure location of indirectly bonded brackets. MATERIALS AND METHODS: Sixty bovine incisors were randomly divided into three groups (n = 20), and their buccal faces were etched using 37% phosphoric acid. In group 1 (control), brackets were indirectly bonded using only Sondhi adhesive. In groups 2 and 3, the adhesion boosters Enhance Adhesion Booster and Assure Universal Bonding Resin, respectively, were applied before bonding with Sondhi. Maximum bond strength was measured with a universal testing machine, and the location of bond failure was evaluated using the Adhesive Remnant Index (ARI). One-way analysis of variance followed by the Tukey test (P < .05) was used to compare the shear bond strength among groups, and the differences in ARI scores were evaluated using the Kruskal-Wallis test (P < .05). The Pearson correlation coefficient was calculated to determine whether there was any correlation between bond strength and ARI scores. RESULTS: The mean shear bond strength in group 3 was significantly higher (P < .01) than in the other groups. Evaluation of the locations of bond failure revealed differences (P < .05) among the three groups. There was a moderate correlation between bond strength and ARI scores within group 3 (r  =  0.5860, P < .01). CONCLUSION: In vitro shear bond strength was acceptable in all groups. The use of the Assure adhesion booster significantly increased both the shear bond strength of indirectly bonded brackets and the amount of adhesive that remained on the enamel after bracket debonding.

CO2 laser for dental alumina ceramic framework welding / O laser de CO2 para a soldagem de infraestruturas cerâmicas de alumina

Objective: Despite the increase of all-ceramic prosthesis in dental practice there is no evidence of the possibility of welding these structures if necessary. The objective of this study was to use CO2 laser (λ = 10.6 µm) as a welding agent to fuse dental polycrystalline alumina ceramic. Methods: Ceramic blocks of pre-sintered alumina were sectioned into 20 bars (10.0 x 1.5 x 1.5 mm) and sintered to the final cross section dimension of 1.2 x 1.2mm. The bars were adapted to an LHPG (Laser Heated Pedestal Growth) system device where the bars could be fixed in pairs and have their ends irradiated with CO2 laser to fusion. The ring-shaped laser beam (300 µm thickness) was directed with the aid of mirrors to reach samples' ends. The laser was continuously applied (40 W nominal power, 5 seconds). After welding, the samples were analyzed in stereomicroscope and SEM. A diffraction analysis was carried out with one sample. Results: CO2 laser was able to fuse the ceramic bars, but some of them showed some shape distortion in the fusion zone. The aspect of the fused alumina differed in color and translucency from the original sintered material. SEM evidenced the presence of porosity and voids in the center of the fusion zone. X-ray diffraction pointed to a reduction in crystallite size by two to four times in the welded region of samples. Conclusions: This study points to CO2 laser as a possible welding agent to polycrystalline alumina dental ceramic. Porosity observed in the molten zone gives cause for concern regarding weld resistance. (AU)

Objetivo: Apesar do aumento no número de próteses totalmente cerâmicas na prática clínica, não há evidência da possibilidade de se soldar essas estruturas se necessário. Este estudo testou o laser de CO2 (λ=10.6 µm) como um agente de solda para estruturas cerâmicas de alumina policristalina. Métodos: Blocos cerâmicos de alumina pré-sinterizada foram seccionados na forma de barras (10,0 x 1,5 x 1,5 mm) e sinterizados na dimensão final de 1,2 x 1,2 mm. As barras foram adaptadas a um sistema de crescimento de fibras cerâmicas (LHPG ­ Laser Heated Pedestal Growth) onde as barras foram fixadas em pares e tiveram suas extremidades irradiadas com laser de CO2 até a sua fusão. O feixe de laser foi direcionado com a ajuda de espelhos para atingir as extremidades das barras. O laser foi aplicado de forma contínua (40 W de potência nominal, 5 segundos). Depois da solda, os espécimes foram analisados em estereomicroscopia e MEV. Teste de difração foi conduzido com um espécime. Resutados: O laser de CO2 foi capaz de fundir as barras cerâmicas, mas algumas delas mostraram uma distorção da forma na zona de fusão. O aspecto da alumina soldada diferiu em cor e translucidez do material original. O MEV evidenciou a presença de porosidade no centro da zona de fusão. A difração por raio-x apontou para uma redução no tamanho dos cristalitos de duas a quatro vezes na região da solda. Conclusões: Esse estudo mostrou ser possível usar o laser de CO2 como um agente de solda para uma cerâmica policristalina de alumina. A porosidade observada na zona de fusão traz preocupação quanto à resistência da solda. (AU)

Porcelain monolayers and porcelain/alumina bilayers reinforced by Al2O3/GdAlO3 fibers.

AIM: This work tested the effect of the addition of Al(2)O(3)/GdAlO(3) longitudinal fibers in different contents to veneering porcelain of two dental all ceramic systems. METHODS: Fibers (0.5 mm diameter) obtained by the Laser Heated Pedestal Growth (LHPG) method were added to bar-shaped specimens made by veneer porcelain (monolayers) or both the veneer and the core ceramic (bilayers) of two all-ceramic systems: In-Ceram Alumina-glass infiltrated alumina composite (GIA) and In-Ceram 2000 AL Cubes-alumina polycrystal (AP) (VITA Zahnfabrik). The longitudinal fibers were added to veneering porcelain (VM7) in two different proportions: 10 or 17 vol%. The bars were divided into nine experimental conditions (n=10) according to material used: VM7 porcelain monolayers, VM7/GIA, VM7/AP; and according to the amount of fibers within the porcelain layer: no fibers, 10 vol% or 17 vol%. After grinding and polishing the specimens were submitted to a three point bending test (crosshead speed = 0.5 mm/min) with porcelain positioned at tensile side. Data were analyzed by means of one-way ANOVA and a Tukey's test (α=5%). Scanning electronic microscopy (SEM) was conducted for fractographic analysis. RESULTS: Regarding the groups without fiber addition, VM7/AP showed the highest flexural strength (MPa), followed by VM7/GIA and VM7 monolayers. The addition of fibers led to a numerical increase in flexural strength for all groups. For VM7/GIA bilayers the addition of 17 vol% of fibers resulted in a significant 48% increase in the flexural strength compared to the control group. Fractographic analysis revealed that the crack initiation site was in porcelain at the tensile surface. Cracks also propagated between fibers before heading for the alumina core. CONCLUSIONS: The addition of 17 vol% of Al(2)O(3)/GdAlO(3) longitudinal fibers to porcelain/glass infiltrated alumina bilayers significantly improved its flexural strength. 10 vol% or 17 vol% of fibers inclusion increased the flexural strength for all groups.

Influence of the Extended Use of Desensitizing Toothpastes on Dentin Bonding, Microhardness and Roughness

Abstract The aim of this study was to evaluate the influence of an extended use of desensitizing toothpastes (DTs) on dentin bonding, microhardness and roughness. One hundred and twenty bovine incisor teeth were randomly divided into four groups: G1, distilled water (WATER); G2, Colgate Total 12 (CT12); G3, Colgate Sensitive Pro-Relief (CSPR); and G4, Sensodyne Repair ?αμπ; Protect (SRP). Dentin surfaces were etched with 17% EDTA and 2 years of simulated tooth brushing (20,000 cycles) was performed on their surfaces. Knoop microhardness, surface roughness and scanning electron microscopy (SEM) were performed before and after the simulated tooth brushing. For microshear bonding test, a 2-step self-etching adhesive system (Clearfil SE Bond) was used and 0.8 mm diameter composite resin (Filtek Z350 XT) cylinders were built. Microshear test was performed with an orthodontic wire and with a crosshead speed of 0.5 mm/min. The data were analyzed for: 1) bond strength (one-way ANOVA), 2) microhardness intra-group (Student's test) and inter-group (one-way ANOVA/Tukey's test) comparisons, 3) roughness intra-group (Student's test/Wilcoxon's test) and inter-group (Kruskal-Wallis/Student-Newman-Keuls test) comparisons. The extended use of both dentifrices (conventional and for sensitive teeth) did not affect the bond strength and produced a significant increase in microhardness and roughness of the dentin, except for the microhardness of the SRP group. The simulated tooth brushing technique with water produced an increase in roughness, without reducing significantly the dentin microhardness.

Resumo O objetivo deste estudo foi avaliar a influência do uso prolongado de dentifrícios dessensibilizantes (DTs) na adesão, microdureza e rugosidade da dentina. Cento e vinte dentes incisivos bovinos foram divididos aleatoriamente em quatro grupos: G1, água destilada (WATER); G2, Colgate Total 12 (CT12); G3, Colgate Sensitive Pro-alívio (CSPR); e G4, Sensodyne Repair ?αμπ; Protect (SRP). As superfícies dentinárias foram condicionadas com EDTA a 17% e foram realizados 2 anos de escovação dental simulada (20.000 ciclos) em suas superfícies. A microdureza Knoop, rugosidade de superfície e microscopia eletrônica de varredura (MEV) foram realizadas antes e após escovação dental simulada. Para o teste de adesão por microcisalhamento, foi aplicado um sistema adesivo auto condicionante de 2 passos (Clearfil SE Bond) e foram construídos cilindros de resina composta (Filtek Z350 XT) de 0,8 mm de diâmetro. O teste de microcisalhamento foi realizado com um fio ortodôntico e com velocidade de 0,5mm/min. Os dados foram analisados para: 1) resistência de união (ANOVA 1 fator), 2) comparações de microdureza intra grupo (teste t de Student) e inter grupo (ANOVA 1 fator / teste de Tukey), 3) comparações de rugosidade intra grupo (teste t de Student / teste de Wilcoxon) e inter grupo (Kruskal Wallis / teste de Student-Newman-Keuls). O uso prolongado de ambos dentifrícios (convencional e para dentes sensíveis) não interferiu na resistência de união e produziu um aumento significativo na microdureza e rugosidade da dentina, exceto para a microdureza do grupo SRP. A técnica de escovação dental simulada com água promoveu aumento na rugosidade, sem reduzir significativamente a microdureza da dentina.

Effect of Glaze Cooling Rate on Mechanical Properties of Conventional and Pressed Porcelain on Zirconia

Abstract The aim of this study was to characterize a conventional and a pressed porcelain for zirconia core as to biaxial flexural strength (BFS), apparent fracture toughness (FT) and microstructure composition, and to investigate the effect of glaze cooling rate on the BFS of the zirconia/porcelain bilayers. Monolayers of conventional porcelain Vita VM9 and pressed porcelain Vita PM9 (n=15) (12 mm diameter x 1.2 mm thick) were prepared for the BFS test (MPa). Apparent fracture toughness (MPa.m1/2) was measured by indentation technique (n=15). t-Student test was performed for statistical analysis. Scanning electron microscopy and x-ray diffraction were used to analyze the porcelain's microstructure. For the BFS of bilayers, zirconia discs (12 mm diameter x 1 mm thick) (Vita In-Ceram YZ) were veneered with the two porcelains (1 mm thick). After the glaze firing simulation, the specimens were submitted to fast or slow cooling (n=15). Apparent fracture toughness (MPa.m1/2) was measured on the porcelain surface of bilayers (n=15) and residual stress was calculated. Two-way ANOVA (porcelain and cooling method) was used for the bilayer analysis (a=0.05). Vita PM9 monolayer exhibited significantly higher BFS (p<0.01), but there was no significant difference (p=0.41) in the FT between the porcelains. For bilayer specimens, the two-way ANOVA for BFS was significant for the porcelain variable only (p<0.01) better for Vita PM9/zirconia. Two-way ANOVA for the FT for the bilayers was not significant for any variable. All groups showed compressive residual stresses. The pressed porcelain seems to be mechanically more effective for zirconia veneering.

Resumo Este estudo teve como objetivo caracterizar uma porcelana convencional e uma prensada indicadas para infraestrutura de zircônia em termos de resistência à flexão biaxial (RFB), tenacidade à fratura aparente (TF) e composição microestrutural, e investigar o efeito da taxa de resfriamento do ciclo de glaze na RFB de espécimes bicamada de zircônia/porcelana. Espécimes monocamada de porcelana convencional Vita VM9 e porcelana prensada Vita PM9 (n=15) (12 mm de diâmetro x 1,2 mm de espessura) foram preparados para o ensaio de RFB (MPa). Tenacidade à fratura aparente (MPa.m1/2) foi mensurada pela técnica da indentação (n=15). O teste de t-Student foi aplicado para as análises estatísticas. Microscopia eletrônica de varredura e difração de raios-X foram utilizadas para análise microestrutural da porcelana. Para o ensaio de RFB nos bicamadas, discos de zircônia (12 mm de diâmetro x 1 mm de espessura) (Vita In-Ceram YZ) foram recobertos com as duas porcelanas (1 mm de espessura). Após a simulação da queima de glaze, os espécimes foram submetidos ao método de resfriamento rápido ou lento (n=15). A tenacidade à fratura aparente (MPa.m1/2) foi mensurado na superfície da porcelana dos bicamadas (n=15) e o estresse residual foi calculado. Foi utilizado ANOVA a dois fatores (porcelana e método de resfriamento) para análise estatística dos espécimes bicamada (a=0,05). Monocamadas de Vita PM9 exibiram valores de resistência à flexão biaxial significativamente maiores (p<0,01), mas não houve diferença estatística (p=0,41) em relação à TF entre as porcelanas. Para os espécimes bicamada, ANOVA a dois fatores para RFB foi significante apenas para a variável porcelana (p<0,01), com superioridade para Vita PM9/zircônia. ANOVA a dois fatores para TF dos espécimes bicamadas não apresentou significância para nenhuma variável. Todos os grupos apresentaram estresse residual de compressão. Porcelanas prensadas parecem ser mecanicamente mais efetivas para recobrimento de zircônia.