RESUMEN
Because of their sensitivity and high level of discrimination, short tandem repeat (STR) maker systems are currently the method of choice in routine forensic casework and data banking, usually in multiplexes up to 15-17 loci. Constraints related to sample amount and quality, frequently encountered in forensic casework, will not allow to change this picture in the near future, notwithstanding the technological developments. In this study, we present a free online calculator named PopAffiliator ( http://cracs.fc.up.pt/popaffiliator ) for individual population affiliation in the three main population groups, Eurasian, East Asian and sub-Saharan African, based on genotype profiles for the common set of STRs used in forensics. This calculator performs affiliation based on a model constructed using machine learning techniques. The model was constructed using a data set of approximately fifteen thousand individuals collected for this work. The accuracy of individual population affiliation is approximately 86%, showing that the common set of STRs routinely used in forensics provide a considerable amount of information for population assignment, in addition to being excellent for individual identification.
Asunto(s)
Computadores/legislación & jurisprudencia , Genética Forense/instrumentación , Genética Forense/legislación & jurisprudencia , Marcadores Genéticos/genética , Genética de Población/legislación & jurisprudencia , Genotipo , Repeticiones de Microsatélite/genética , Grupos de Población/genética , Inteligencia Artificial , Frecuencia de los Genes/genética , HumanosRESUMEN
POPULATION: One hundred and thirteen unrelated Flemish men from Belgium.
Asunto(s)
Cromosomas Humanos Y , Genética de Población , Haplotipos , Secuencias Repetidas en Tándem , Bélgica , Dermatoglifia del ADN , Humanos , MasculinoRESUMEN
A 37-year-old Belgian patient presented with acute nephropathia epidemica (NE) shortly after a camping holiday in southern France. Unusual symptoms were initial noncardiogenic lung involvement, followed by severe acute renal failure, acute acalculous cholecystitis, presence of immunoblasts in the bone marrow, and hemolytic anemia, presenting as hemolytic uremic syndrome. Positive immunoglobulin (Ig) A and rising IgG titers against Puumala hantavirus (PUUV) were detected, but IgM remained negative on days 8 and 20. The results of reverse-transcriptase-polymerase chain reaction performed on day 8 were positive for PUUV. This is the first report of an iatrogenically IgM-negative hantavirus case due to the selective removal of heavy-weight molecules during plasma exchange via the centrifugation technique. This is also the first report of proven NE from the Mediterranean part of France.
Asunto(s)
Infecciones por Hantavirus/inmunología , Fiebre Hemorrágica con Síndrome Renal/inmunología , Inmunoglobulina M/inmunología , Orthohantavirus , Adulto , Ensayo de Inmunoadsorción Enzimática , Francia , Fiebre Hemorrágica con Síndrome Renal/virología , Humanos , Enfermedad Iatrogénica , MasculinoRESUMEN
Evolutionary medicine is the application of evolution theory to understanding health and disease. It provides a complementary scientific approach to the present mechanistic explanations that dominate medical science, and particularly medical education. The chronic multifactorial disease of atherosclerosis clearly illustrates the Darwinian paradigm. Recent research, combining the effects of genes and environment, has provided surprising clues to the pathogenesis of this major public health problem. This example makes a strong case for recognizing evolution biology as a basic science for medicine.