RESUMEN
Anaerobic digestion (AD) of antibiotic manufacturing wastewater to degrade residual antibiotics and produce mixture of combustible gases has been investigated actively in the past decades. However, detrimental effect of residual antibiotic to microbial activities is commonly faced in AD process, leading to the reduction of treatment efficiency and energy recovery. Herein, the present study systematically evaluated the detoxification effect and mechanism of Fe3O4-modified biochar in AD of erythromycin manufacturing wastewater. Results showed that Fe3O4-modified biochar had stimulatory effect on AD at 0.5 g/L erythromycin existence. A maximum methane yield of 327.7 ± 8.0 mL/g COD was achieved at 3.0 g/L Fe3O4-modified biochar, leading to the increase of 55.7% compared to control group. Mechanistic investigation demonstrated that different levels of Fe3O4-modified biochar could improve methane yield via different metabolic pathways involved in specific bacteria and archaea. Low levels of Fe3O4-modified biochar (i.e., 0.5-1.0 g/L) led to the enrichment of Methanothermobacter sp., strengthening the hydrogenotrophic pathway. On the contrary, high levels of Fe3O4-modified biochar (2.0-3.0 g/L) favored the proliferation of acetogens (e.g., Lentimicrobium sp.) and methanogen (Methanosarcina sp.) and their syntrophic relations played vital role on the simulated AD performance at erythromycin stress. Additionally, the addition of Fe3O4-modified biochar significantly decreased the abundance of representative antibiotic resistant genes (ARGs), benefiting the reduction of environmental risk. The results of this study verified that the application of Fe3O4-modified biochar could be an efficient approach to detoxify erythromycin on AD system, which brings high impacts and positive implications for biological antibiotic wastewater treatment.
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Eritromicina , Aguas Residuales , Eritromicina/farmacología , Anaerobiosis , Carbón Orgánico , Antibacterianos/farmacología , Metano , Reactores BiológicosRESUMEN
The in-situ hydrogen supply by nano zero-valent iron (nZVI, nFe0) corrosion provided a feasible way to improve the efficiency of biogas biological upgrading. This work studied the effects of nZVI at different dosages (0, 2, 4, 6, 8 and 10 g/L) on anaerobic digestion of kitchen wastewater by two buffer systems 2-[4-(2-hydroxyethyl) piperazin-1-yl] ethanesulfonic acid (HEPES) and sodium hydrogen carbonate (NaHCO3). The addition of nZVI improved the content of methane (CH4) and stability of anaerobic digestion process. In HEPES buffer system, the CH4 was all increased and the maximum reached 90.51% with 10 g/L nZVI, higher than 32.25% compared to the control. The maximum hydrogen enrichment (HE) was 113 ppb after nZVI addition, indicating the mass transfer efficiency of hydrogen (H2) was improved. Microbial community analysis showed that the total relative abundance of Methanobacterium and Methanolinea at 10 g/L nZVI was 53.72%, which was 1.62 times of the control group. However, in the NaHCO3 buffer system with 10 g/L nZVI addition, the content of CH4 and the loosely bound extracellular polymeric substances (LB-EPS) was lower than the control. The results indicated that the addition of nZVI was feasible for biogas upgrading, and the bidirectional effect of nZVI on the promotion or inhibition of bio-methanation might be related to the buffer system of the anaerobic process.
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Biocombustibles , Aguas Residuales , Anaerobiosis , Biocombustibles/análisis , Corrosión , Hidrógeno , Hierro , Metano/metabolismo , Aguas del Alcantarillado/microbiologíaRESUMEN
Algal organic matter (AOM) and natural organic matter (NOM) from a typical eutrophic lake were comprehensively investigated in terms of their physico-chemical property, components and disinfection byproduct formation potentials (DBPFPs). The relationships between specific chemical properties of AOM and NOM with their corresponding DBPFPs were further evaluated during chlorination. Results indicated that AOM had lower specific UV absorbance (SUVA) but richer organic nitrogen contents than NOM. Fluorescence excitation emission matrix spectroscopy further demonstrated that AOM were chiefly composed of aromatic protein-like and soluble microbial byproduct-like matters, while NOM were mainly contributed from humic acid-like and soluble microbial byproduct-like substances. Although the molecular weight (MW) distribution of AOM and NOM showed no significant difference, size-exclusion chromatography with organic carbon as well as organic nitrogen detection (LC-OCD-OND) revealed that AOM were concentrated with the fraction of building blocks and NOM had higher concentrations of biopolymers and humics (HS). Moreover, AOM displayed higher DBPFPs than NOM, especially for nitrogenous DBPFP (N-DBPFP). MW < 1 kDa fractions both in AOM and NOM contributed the largest proportion to the formation of carbonaceous disinfection byproducts (C-DBPs). In addition, Pearson correlation analysis showed that bulk parameter SUVA was significantly relevant to the formation potentials of trihalomethane both in AOM and NOM, but was ineffective for carbonaceous DBPFP (C-DBPFP) prediction. Dissolved organic nitrogen contents in biopolymer and HS characterized by LC-OCD-OND had strong correlations with N-DBPFPs from AOM and NOM, indicating that LC-OCD-OND quantitative analysis could improve the prediction accuracy of the DBP formation than bulk parameters during NOM and AOM chlorination.
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Contaminantes Químicos del Agua , Purificación del Agua , Desinfección , Halogenación , Nitrógeno/análisis , Trihalometanos/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
The widespread existence of microplastics in wastewater has caused great concern. As the exposure time of microplastics in the environment increases, the microplastic leaching solution (i.e.,chemical additives) may be released into the environment causing toxic effects. In this study, the effect of polystyrene (PS) microplastics on the anaerobic digestion system was investigated. The results showed that the exposure to 80 nm and 5 µm polystyrene microplastics with the concentrations of 0.2 g/L or lower did not significantly affect the cumulative methane production (P ≥ 0.05). On the other hand, 80 nm and 5 µm PS microplastic level of 0.25 g/L led to a decrease in methane production by 19.3% (P = 2 × 10-5) and 17.9% (P = 4 × 10-5), respectively. The 80 nm PS nanoplastics therefore had slightly higher inhibition capacity on methane production than 5 µm PS microplastics. The pH of all groups remained stable at 6.7-7.5. Volatile fatty acids (VFAs) concentration and ammonium-nitrogen concentration had no obvious relationship to PS micro and nanoplastics addition. Further investigation showed that PS micro and nanoplastics concentration of 0.25 g/L or higher could inhibit acidification and methanation stage of anaerobic digestion. However, the main negative influence of PS micro and nanoplastics on methane production was due to the severe inhibition on the methanization stage.
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Microplásticos , Poliestirenos , Eliminación de Residuos Líquidos/métodos , Anaerobiosis , Metano , Plásticos , Aguas ResidualesRESUMEN
Using lignocellulosic materials as substrates, ruminal microbiota were co-inoculated with anaerobic sludge at different loading rates (LR) to study the microbial community in the semi-continuous mode. The results indicated that the highest CH4 yield reached 0.22 L/g volatile solid at LR of 4 g/L/day, which obtained 56-58% of the theoretical value. In the steady stage with LR of 2-4 g/L/day and slurry recirculation, copies of total archaea increased. Especially the Methanobacteriales increased significantly (p < 0.05) to 3.30 × 108 copies/mL. The microbial communities were examined by MiSeq 16S rRNA sequencing. Enriched hydrolytic bacteria mainly belonged to Clostridiales, including Ruminococcus, Ruminiclostridium, and Ruminofilibacter settled in the rumen. High-active cellulase and xylanase were excreted in the co-inoculated system. Acid-producing bacteria by fermentation were affiliated with Lachnospiraceae and Bacteroidales. The acidogen members were mainly Spirochaetaceae and Clostridiales. Syntrophic oxidation bacteria mainly consisted of Synergistetes, propionate oxidizers (Syntrophobacter and Pelotomaculum), and butyrate oxidizers (Syntrophus and Syntrophomonas). There had no volatile fatty acid (VFA) accumulation and the pH values varied between 6.94 and 7.35. At LR of 6 g/L/day and a recirculation ratio of 1:1, the hardly degradable components and total VFA concentrations obviously increased. The total archaea and Methanobacteriales then deceased significantly to 8.56 × 105 copies/mL and 4.14 × 103 copies/mL respectively (p < 0.05), which resulted in the inhibition of methanogenic activities. Subsequently, microbial diversity dropped, and the hydrolytic bacteria and syntrophic oxidizers obviously decreased. In contrast, the abundances of Bacteroidales increased significantly (p < 0.05). Acetate, propionate, and butyrate concentrations reached 2.02, 6.54, and 0.53 g/L, respectively, which indicated "acidification" in the anaerobic reactor. Our study illustrated that co-inoculated anaerobic sludge enriched the ruminal function consortia and hydrogenotrophic methanogens played an important role in anaerobic digestion of lignocelluloses.
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Fenómenos Fisiológicos Bacterianos , Euryarchaeota/fisiología , Consorcios Microbianos/fisiología , Rumen/microbiología , Anaerobiosis , Animales , Archaea/crecimiento & desarrollo , Archaea/fisiología , Bacterias/clasificación , Euryarchaeota/genética , Lignina/metabolismo , Polisacáridos/metabolismo , ARN Ribosómico 16S/genética , Aguas del Alcantarillado/microbiologíaRESUMEN
The sludge-shifting sequencing batch reactor (SBR) is an enhanced biological phosphorus removal (EBPR) process for wastewater treatment. In this study, the enrichment of phosphorus accumulating organisms (PAOs) will be attempted by using different high concentration of substrates. In sludge-shifting SBR, activated sludge can be continuously shifted from the bottom of SBR to anaerobic zone/selector, which contains high concentration of substrates, through an orderly reflux between the paralleled SBRs. Denaturing gradient gel electrophoresis (DGGE) methods were used to monitor microbial diversity in sludge. Fluorescence in situ hybridization (FISH) was used to determine the microbial population profile and distribution map under different sludge shifting volumes. The synthesis of intracellular polymers in this process was also analyzed. Phosphorus removal efficiency as high as 96% ± 1.3% was achieved under a sludge shifting ratio of 30%. Synthetic efficiencies of polyhydroxybutyrate (PHB) by PAOs were improved at high sludge shifting ratios. FISH results demonstrated that the population of PAOs in the process increased under properly sludge shifting ratio and it significantly improved phosphorus removal efficiency. Sequencing results indicated that determined sequences (11 OTUs) belonged to Proteobacterium, Actinobacteria and Firmicutes, Pseudomonas kuykendallii, which played an important role in the process of P removal.
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Reactores Biológicos , Fósforo , Aguas del Alcantarillado , Hibridación Fluorescente in Situ , Dinámica Poblacional , Eliminación de Residuos LíquidosRESUMEN
In this study, a laboratory-scale system combined a vertical flow constructed wetland (VF) with a horizontal flow constructed wetland (HF), which was used to treat the secondary effluent of a wastewater treatment plant. Removal efficiencies of 67.02%, 89.80%, 90.31% and 75.38% were achieved by the system for chemical oxygen demand (COD), ammonium nitrogen (NH4 +-N), total nitrogen (TN) and total phosphorus (TP), respectively. The VF showed much higher average loading rates of COD, TP, NH4 +-N and TN (7.96 g/m2/d, 0.076 g/m2/d, 0.31 g/m2/d and 0.99 g/m2/d) than in HF (0.65 g/m2/d, 0.016 g/m2/d, 0.25 g/m2/d and 0.50 g/m2/d), during the stable operation period. Biodegradation played a major role in pollutant removal, especially for COD and TN. The results of bacterial community analysis indicated that heterotrophic denitrifying bacteria (Hydrogenophaga and Flavobacterium) were the dominant contributors for nitrogen removal in the VF, while heterotrophic denitrifying bacteria (Rhodobacter, Flavobacterium and Dechloromonas) and the autotrophic denitrifying bacteria Sulfurimonas played the principal roles for nitrogen removal in the HF. Redundancy analyses showed that COD and NH4 +-N were the important factors affecting the distribution of nitrogen removal bacteria in the VF, while pH, dissolved oxygen and oxidation-reduction potential were the key factors influencing the distribution of nitrogen removal bacteria in the HF.
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Bacterias/genética , Eliminación de Residuos Líquidos/métodos , Aguas Residuales/química , Humedales , Bacterias/clasificación , Bacterias/metabolismo , Biodegradación Ambiental , Análisis de la Demanda Biológica de Oxígeno , Desnitrificación , Nitrógeno/análisis , Fósforo/análisisRESUMEN
Disinfection byproduct (DBP) precursors in wastewater during the reversed anaerobic-anoxic-oxic (A2/O) process, as well as their molecular weight (MW) and polarity-based fractions, were characterized with UV scanning, fluorescence excitation emission matrix, Fourier transform infrared and nuclear magnetic resonance spectroscopy. Their DBP formation potentials (DBPFPs) after chlorination were further tested. Results indicated that the reversed A2/O process could not only effectively remove the dissolved organic carbon (DOC) and dissolved total nitrogen in the wastewater, but also affect the MW distribution and hydrophilic-hydrophobic properties of dissolved organic matter (DOM). The accumulation of low MW and hydrophobic (HPO) DOM was possibly due to the formation of soluble microbial product-like (SMP-like) matters in the reversed A2/O treatment, especially in the anoxic and aerobic processes. Moreover, DOM in the wastewater displayed a high carbonaceous disinfection byproduct formation potential (C-DBPFP) in the fractions of MW>100kDa and MW<5kDa, and revealed an increasing tendency of nitrogenous disinfection byproduct formation potential (N-DBPFP) with decrease of MW. For polarity-based fractions, the HPO fraction of wastewater showed significantly higher C-DBPFP and N-DBPFP than hydrophilic and transphilic fractions. Therefore, although the reversed A2/O process could remove most DBP precursors by DOC reduction, it led to the enhancement of DBPFP with the formation and accumulation of low MW and HPO DOM. In addition, strong correlations between C-DBPFPs and SUVA, and between N-DBPFPs and DON/DOC, were observed in the wastewater, which might be helpful for DBPFP prediction in wastewater and reclaimed water chlorination.
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Desinfectantes/análisis , Eliminación de Residuos Líquidos/métodos , Contaminantes Químicos del Agua/análisis , Desinfección/métodos , Interacciones Hidrofóbicas e Hidrofílicas , Nitrógeno , Trihalometanos/análisis , Aguas Residuales/químicaRESUMEN
In this study, a full-scale internal circulation (IC) reactor coupled with an external circulation system was developed to treat high-strength leachate from a municipal solid waste (MSW) incineration plant, in which anaerobic sludge granulation was intensively investigated. Results showed that the IC reactor achieved excellent treatment performance under high organic loading rates (OLR) of 21.06-25.16kg chemical oxygen demand (COD)/(m3âday). The COD removal efficiency and biogas yield respectively reached 89.4%-93.4% and 0.42-0.50m3/kgCOD. The formation of extracellular polymeric substances (EPS) was closely associated with sludge granulation. Protein was the dominant component in sludge EPS, and its content was remarkably increased from 21.6 to 99.7mg/g Volatile Suspended Solid (VSS) during the reactor operation. The sludge Zeta potential and hydrophobicity positively correlated with the protein/polysaccharide ratio in EPS, and they were respectively increased from -26.2mV and 30.35% to -10.6mV and 78.67%, which was beneficial to microbial aggregation. Three-dimensional fluorescence spectroscopy (3D-EEM) and Fourier transform infrared spectroscopy (FT-IR) analysis further indicated the importance of protein-like EPS substances in the sludge granulation. Moreover, it was also found that the secondary structures of EPS proteins varied during the reactor operation.
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Reactores Biológicos , Eliminación de Residuos Líquidos/métodos , Anaerobiosis , Análisis de la Demanda Biológica de Oxígeno , Metano/análisis , Aguas del Alcantarillado/químicaRESUMEN
Ruminal microbiota (RM) were co-inoculated with anaerobic sludge (AS) at different ratios to study the digestion of rice straw in batch experiments. The CH4 yield reached 273.64 mL/g volatile solid (VS) at a co-inoculum ratio of 1:1. The xylanase and cellulase activities were 198.88-212.88 and 24.51-29.08 U/mL in co-inoculated samples, respectively, and were significantly different compared to the results for single inoculum (p < 0.05). Higher ratios of AS enhanced acetoclastic methanogenesis, and propionate accumulation could be the main reason for the longer lag phase observed in samples with a higher RM ratio. The microbial compositions were clearly altered after digestion. Fibrobacter, Ruminococcus and Butyrivibrio from the rumen did not settle in the co-inoculated system, whereas Clostridiales members became the main polysaccharide degraders. Microbial interactions involving hydrolytic bacteria and acetoclastic methanogens in the residue were considered to be significant for hydrolysis activities and methane production. Syntrophy involving propionate oxidizers with associated methanogens occurred in the liquid phase. Our findings provide a better understanding of the anaerobic digestion of rice straw that is driven by specific microbial populations.
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Consorcios Microbianos/fisiología , Microbiota , Oryza , Rumen/microbiología , Aguas del Alcantarillado/microbiología , Anaerobiosis , Animales , Butyrivibrio/aislamiento & purificación , Celulasa/metabolismo , Clostridiales/aislamiento & purificación , Endo-1,4-beta Xilanasas/metabolismo , Fibrobacter/aislamiento & purificación , Hidrólisis , Metano/biosíntesis , Tallos de la Planta/metabolismo , Propionatos/metabolismo , Ruminococcus/aislamiento & purificaciónRESUMEN
Anaerobic ammonium oxidation (Anammox) has become a promising method for biological nitrogen removal. However, this biotechnology application is always limited due to the low growth rate and biomass yield of Anammox bacteria. This study investigated the process of fast reactivation of an Anammox consortium idled for 2 years via hydrodynamic stress control. The results showed that the Anammox system was efficiently and quickly reactivated by shortening of the hydraulic retention time (HRT) of the reactor from 12 to 6 hr within 68 days of operation. Moreover, at a 4-hr HRT with an influent total nitrogen loading rate of 1.2kg N/(m(3)·day), the reactor maintained high biological performance with an ammonium removal loading rate of 0.52kg N/(m(3)·day) and a nitrite removal rate of 0.59kg N/(m(3)·day). In the reactivated Anammox reaction, the stoichiometric coefficients of NH4(+)-N to NO2(-)-N and NH4(+)-N to NO3(-)-N were 1:1.04±0.08 and 1:0.31±0.03, respectively. The specific Anammox activity and hydrazine oxidoreductase activity, both of which represent the degree of Anammox bacteria present, increased as the hydrodynamic stress increased and were maximally (125.38±3.01mg N/(g VSS·day) and 339.42±6.83µmol/(min·g VSS), respectively) at 4-hr HRT. Microbial response analysis showed that the dominant microbial community was obviously shifted and the dominance of Anammox bacteria was enhanced during the hydrodynamic selection.
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Compuestos de Amonio/metabolismo , Reactores Biológicos/microbiología , Consorcios Microbianos , Nitrógeno/aislamiento & purificación , Anaerobiosis , Oxidación-ReducciónRESUMEN
Ecological risk of micro/nano-plastics (MPs/NPs) has become an important environmental issue. Microcystin-leucine-arginine (MC-LR) produced by Microcystis aeruginosa (M. aeruginosa) is the most common and toxic secondary metabolites (SM). However, the influencing mechanism of MPs and NPs exposure on MC-LR synthesis and release have still not been clearly evaluated. In this work, under both acute (4d) and long-term exposure (10d), only high-concentration (10 mg/L) exposure of amino-modified polystyrene NPs (PS-NH2-NPs) promoted MC-LR synthesis (32.94 % and 42.42 %) and release (27.35 % and 31.52 %), respectively. Mechanistically, PS-NH2-NPs inhibited algae cell density, interrupted pigment synthesis, weakened photosynthesis efficiency, and induced oxidative stress, with subsequent enhancing the MC-LR synthesis. Additionally, PS-NH2-NPs exposure up-regulated MC-LR synthesis pathway genes (mcyA, mcyB, mcyD, and mcyG) combined with significantly increased metabolomics (Leucine and Arginine), thereby enhancing MC-LR synthesis. PS-NH2-NPs exposure enhanced the MC-LR release from M. aeruginosa via up-regulated MC-LR transport pathway genes (mcyH) and the shrinkage of plasma membrane. Our results provide new insights into the long-time coexistence of NPs with algae in freshwater systems might pose a potential threat to aquatic environments and human health.
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Toxinas Marinas , Microcistinas , Microcystis , Poliestirenos , Microcystis/metabolismo , Microcystis/efectos de los fármacos , Microcistinas/metabolismo , Microcistinas/toxicidad , Poliestirenos/toxicidad , Poliestirenos/química , Fotosíntesis/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Arginina/química , Arginina/metabolismo , Nanopartículas/toxicidad , Nanopartículas/química , Microplásticos/toxicidadRESUMEN
Biological treatment of food waste (FW) by black soldier fly larvae (BSFL) is considered as an effective management strategy. The composition and concentrations of nutrients in FW change during its storage and transport period, which potentially affect the FW conversion and BSFL growth. The present study systematically investigated the effect of different storage times (i.e., 0-15 d) on FW characteristics and its substantial influence on the BSFL growth. Results showed that the highest larvae weight of 282â¯mg and the shortest growth time of 14â¯days were achieved at the group of FW stored for 15â¯days, but shorter storage time (i.e., 2-7 d) had adverse effect on BSFL growth. Short storage time (i.e., 2-4 d) improved protein content of BSFL biomass and prolonged storage time (i.e., 7-10 d) led to the accumulation of fat content. The changes of substrate characteristics and indigenous microorganisms via FW storage time were the main reasons for BSFL growth difference. Lactic acid (LA) accumulation (i.e., 19.84â¯g/L) in FW storage for 7â¯days significantly limited the BSFL growth, leading to lowest larvae weight. Both the substrate and BSFL gut contained same bacterial communities (e.g., Klebsiella and Proteus), which exhibited similar change trend with the prolonged storage time. The transfer of Clostridioides from substrate to BSFL gut promoted nutrients digestion and intestinal flora balance with the FW stored for 15â¯days. Pathogens (e.g., Acinetobacter) in BSFL gut feeding with FW storage time of 7â¯days led to the decreased digestive function, consistent with the lowest larvae weight. Overall, shorter storage time (i.e., 2-7 d) inhibited the BSFL digestive function and growth performance, while the balance of the substrate nutrients and intestinal flora promoted the BSFL growth when using the FW stored for 15â¯days.
RESUMEN
This study explored the feasibility of calcium peroxide (CaO2) to inhibit cyanobacterial blooms of the outbreak and dormancy stages. Our previous studies have found that CaO2 has a high inhibitory effect on cyanobacteria. In order to explore the application effect of CaO2 in actual cyanobacteria lake water, we conducted this study to clarify the effect of CaO2 on inhibiting cyanobacteria in outbreak and dormancy stages. The results showed that CaO2 inhibited the growth of cyanobacteria in the outbreak and dormancy stages by 98.7% and 97.6%, respectively. The main inhibitory mechanism is: (1) destroy the cell structure and make the cells undergo programmed cell death by stimulating the oxidation balance of cyanobacteria cells; (2) EPS released by cyanobacteria resist stimulation and combine calcium to form colonies, and accelerate cell settlement. In addition to causing direct damage to cyanobacteria, CaO2 can also improve water quality and sediment microbial diversity, and reduce the release of sediment to phosphorus, so as to further contribute to cyanobacterial inhibition. Finally, the results of qRT-PCR analysis confirmed the promoting effect of CaO2 on the downregulation of photosynthesis-related genes (rbcL and psaB), microcystn (mcyA and mcyD) and peroxiredoxin (prx), and verified the mechanism of CaO2 inhibition of cyanobacteria. In conclusion, this study provides new findings for the future suppression of cyanobacterial bloom, by combining water quality, cyanobacterial inhibition mechanisms, and sediment microbial diversity.
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Cianobacterias , Microbiota , Calidad del Agua , Lagos/microbiología , Fósforo/farmacología , Fósforo/análisis , EutrofizaciónRESUMEN
A laboratory-scale internal circulation (IC) anaerobic reactor fed with brewery wastewater was operated at 35 degrees C + 1 degrees C. The influent was pumped into the bottom of the IC reactor by a pulse pump, whereas the effluent was drawn from the upper outlet and allowed to flow into the effluent tank. The biogas volume was recorded using a gas container connected to a biogas metre. The results indicated that the maximum organic loading rate (OLR) of the IC reactor was 19.5 kg chemical oxygen demand (COD)/m3/day; at which point, the dominant archaeal populations found in the sludge using the polymerase chain reaction with denaturing gradient gel electrophoresis were Methanosaeta species. The COD removal efficiencies of the reactor exceeded 85%, with a maximum specific methane production rate of 210 mL CH4/g volatile suspended solids (VSS)/day and a coenzyme F420 content of 0.16 micromol/g VSS, respectively. The main archaeal species in the sludge samples at different OLRs varied greatly, as compared with the organisms in the inoculated sludge. The dominant archaeal species in the treated sludge at low OLRs were Methanosarcina species, whereas those at high OLRs were Methanosaeta species.
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Reactores Biológicos/microbiología , Methanosarcina/metabolismo , Methanosarcinales/metabolismo , Aguas del Alcantarillado/microbiología , Anaerobiosis , Biocombustibles/análisis , Biocombustibles/microbiología , Análisis de la Demanda Biológica de Oxígeno , Metano/análisis , Metano/metabolismo , Methanosarcina/aislamiento & purificación , Methanosarcinales/aislamiento & purificación , Aguas del Alcantarillado/análisis , Eliminación de Residuos Líquidos/métodosRESUMEN
Two identical full-scale biogas-lift reactors treating brewery wastewater were inoculated with different types of sludge to compare their operational conditions, sludge characteristics, and kinetic models at a mesophilic temperature. One reactor (R1) started up with anaerobic granular sludge in 12 weeks and obtained a continuously average organic loading rate (OLR) of 7.4 kg chemical oxygen demand (COD)/(m3 x day), COD removal efficiency of 80%, and effluent COD of 450 mg/L. The other reactor (R2) started up with residual activated sludge in 30 weeks and granulation accomplished when the reactor reached an average OLR of 8.3 kg COD/(m3 x day), COD removal efficiency of 90%, and effluent COD of 240 mg/L. Differences in sludge characteristics, biogas compositions, and biogas-lift processes may be accounted for the superior efficiency of the treatment performance of R2 over R1. Grau second-order and modified StoverKincannon models based on influent and effluent concentrations as well as hydraulic retention time were successfully used to develop kinetic parameters of the experimental data with high correlation coefficients (R2 > 0.95), which further showed that R2 had higher treatment performance than R1. These results demonstrated that residual activated sludge could be used effectively instead of anaerobic granular sludge despite the need for a longer time.
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Biocombustibles , Reactores Biológicos , Residuos Industriales , Aguas del Alcantarillado , Eliminación de Residuos Líquidos/métodos , Anaerobiosis , Industria de Alimentos , Cinética , Modelos QuímicosRESUMEN
Dissolved organic matter (DOM) is reported to be a precursor to disinfection by-products (DBPs), which have adverse effects on human health. Therefore, it is crucial to effectively remove DOM before water disinfection. Characteristics of DOM and DBPs formation during advanced treatment processes including coagulation, adsorption, ultraviolet (UV) irradiation, and ozone (O3) oxidation in municipal secondary effluent were investigated in this research. DOM was characterized by Fourier transform infrared spectroscopy (FTIR), excitation-emission matrix fluorescence spectroscopy (EEM), and Orbitrap mass spectrometry (Orbitrap MS). Moreover, DBPs formation potential under different advanced treatment processes was also discussed. FTIR results indicated that various functional groups existing in DOM may react with the disinfectant to form toxic DBPs. EEM analysis indicated that DOM in all water samples was dominated by soluble microbial product-like (SMPs) and humic acid-like (HA) substances. The municipal secondary effluent was abundant with DOM and rich in carbon, hydrogen, oxygen, and nitrogen atoms, contained a certain dosage of phosphorus and sulfur atoms, and the highest proportion is lignin. Most of the precursors (CHO features) had positive double bond equivalent subtracted oxygen per carbon [(DBE-O)/C] and negative carbon oxidation state (Cos) in all four different advanced treatment processes. DBPs formation potential (DBPFP) of coagulation, adsorption, UV irradiation, and O3 oxidation advanced treatment processes were 487 µg L-1, 586 µg L-1, 597 µg L-1, and 308 µg L-1, respectively. And the DBPs precursors removal efficiency of coagulation, adsorption, UV irradiation, and O3 oxidation advanced treatment processes were 50.8%, 40.8%, 39.8%, and 69.0%, respectively. This study provides in-depth insights into the changes of DOM in municipal secondary effluent at the molecular level and the removal efficiency of DBPs precursors during coagulation, adsorption, UV irradiation, and O3 oxidation advanced treatment processes.
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Ozono , Contaminantes Químicos del Agua , Purificación del Agua , Humanos , Desinfección/métodos , Materia Orgánica Disuelta , Purificación del Agua/métodos , Contaminantes Químicos del Agua/análisis , Agua , Espectrometría de Masas , Ozono/análisis , CarbonoRESUMEN
The improper disposal of sludge is a still-present phenomenon in China. The leachate formed at the bottom of the sludge pile would cause toxicological effects on aquatic organisms and affect the health of humans by entering the surrounding rivers and groundwater. In this study, the hepatotoxicity of zebrafish induced by sludge leachate was assessed by antioxidant enzyme activities, semi-quantitative histopathological assessment, and TUNEL apoptosis assay. The results indicated that the leachate would induce oxidative stress and eventually lead to an increase in lipid peroxide after a 7-day exposure. The histopathological indexes demonstrated that exposure to leachate would cause histological damage by circulatory disturbances, regressive changes, progressive changes, and inflammatory responses. According to the TUNEL results, it could be inferred that apoptotic hepatocytes increased after exposure for 7 days due to oxidative stress and histological damage. Overall, this study provided a valuable approach to assessing the toxic effects of sludge leachate and described the underlying mechanism of leachate-induced hepatotoxicity in zebrafish. This work will generate new insights into the ecological toxicity of leachate and promote the development of sludge disposal in China.
Asunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas , Contaminantes Químicos del Agua , Humanos , Animales , Antioxidantes/metabolismo , Aguas del Alcantarillado , Pez Cebra/metabolismo , Apoptosis , Contaminantes Químicos del Agua/análisisRESUMEN
Microbial electrolysis cell (MEC) system to treat wastewater containing antibiotics has been researched actively in past years. However, the fate of antibiotic resistant genes (ARGs) in MEC is not fully revealed. The effect of applied voltage on the migration of ARGs between anolyte and biofilm microbes via examining the microbial physiology and abundances of macrolide resistance genes (MRGs) and mobile genetic elements (MGEs) was elucidated in this research. Results showed that the abundance of MRGs and MGEs was decreased in the anolyte, but their abundances were increased on the electrode biofilm, indicating their transmission from anolyte to biofilm microbes. Increased applied voltage enhanced adenosine triphosphate (ATP), reactive oxygen species (ROS), and cell membrane permeability of electrode microorganisms. The structure of the electrode microbial community was shifted through applied voltage, and the abundance of electroactive microorganisms (Geobacter, Azospirillum and Dechlorobacter) was significantly improved. Network analysis revealed that Geobacter and Geothrix were potential hosts for MRGs. Therefore, the horizontal and vertical gene transfer of ARGs could be increased by the applied voltage, leading to the enriched ARGs at the electrode biofilm. This study provides evidence and insights into the transmission of ARGs between anolyte and biofilm microbes in MEC system. SYNOPSIS: This study revealed the effect of applied voltage on ARGs in MEC and the potential migration mechanism of ARGs.
Asunto(s)
Antibacterianos , Genes Bacterianos , Antibacterianos/farmacología , Farmacorresistencia Bacteriana/genética , Macrólidos , ElectrólisisRESUMEN
To alleviate the problems of eutrophication and blue algae accumulation in water, biochar was prepared from blue algae dehydrated using polymerized ferrous sulfate(PFS) to absorb phosphate in water, and the biochar was activated using steam to adjust the pore structure. The preparation conditions of blue algae biochar were optimized using the response surface method. The optimal results were as follows:the dosage of PFS was 458 mg·L-1, the carbonization temperature was 433â, and the mass ratio of biochar precursor to steam was 1:11. Biochar without PFS(F0H11-433) and biochar with PFS(F458H11-433) were characterized using X-ray diffraction(XRD), Fourier-transform infrared spectroscopy(FTIR), zeta potential, and Raman spectra(Raman) were used to study whether blue algae biochar and PFS had a synergic effect on phosphate removal. The results showed that:compared with F0H11-433, iron oxide appeared on the surface, the zero point of charge(pHpzc) increased from 4.41 to 6.19, and the disorder and defect degree of biochar was increased in F458H11-433. The pseudo-second-order model and Langmuir model were suitable for describing the adsorption process of F458H11-433, and the saturated adsorption capacity was 31.97 mg·g-1. F458H11-433 had excellent phosphorus removal efficiency in actual lake water, and the residual phosphate content of effluent was less than 0.025 mg·L-1. In the presence of several common anions, it still showed excellent selective adsorption. After five cycles, the phosphate removal of F458H11-433 still reached 75.78%, indicating that F458H11-433 had the characteristic of being renewable. Combined with the material characterization results before and after adsorption, the phosphorus removal mechanism of F458H11-433 mainly involved electrostatic attraction and ligand exchange.