Impact of Environment and Ontogeny on Relative Fecundity and Egg Quality of Female Oysters (Crassostrea virginica) from Four Sites in Northern Chesapeake Bay.

Resource allocation to reproduction is a primary physiological concern for individuals, and can vary with age, environment, or a combination of both factors. In this study we quantified the impact of environment and individual age on the reproductive output of female oysters Crassostrea virginica. We determined the relative fecundity, egg total lipid content, and overall and omega-3/omega-6 (ω3/ω6) fatty acid signatures (FAS) of eggs spawned by female oysters over a 2-year period (n = 32 and n = 64). Variation was quantified spatially and ontogenetically by sampling young and old oyster populations from two rivers in Chesapeake Bay, totaling four collection sites. During Year 1, when oysters underwent oogenesis in different locations, overall and ω3/ω6 egg FAS varied significantly by river, with no significant differences observed in the FAS of oysters by age in Year 1. In Year 2, when oysters from different sites underwent oogenesis in a single location, no significant differences in the overall egg FAS or ω3/ω6 egg FAS by river or age were observed. These findings suggest that oysters integrate environment into their reproductive output, but that time spent growing at a specific location (in this case, represented by oyster age) plays a relatively minor role in the biochemical composition of oyster eggs. These results have consequences for our understanding of how resources are allocated from the female oyster to eggs and, more generally, the impact of environment and ontogeny on reproductive physiology.

Understanding associations between nitrogen and carbon isotopes and mercury in three Ammodramus sparrows.

We analyzed nitrogen and carbon stable isotope ratios and mercury (Hg) in breast feathers from three species of closely related sparrows, Saltmarsh, Seaside, and Nelson's Sparrows (Ammodramus caudacutus, A. maritimus, and A. nelsoni, respectively), to assess if trophic position and food web structure influence Hg exposure in these species. Sparrows were captured during the non-breeding season from 2006 to 2008 in North Carolina salt marshes near Wrightsville Beach, New Hanover County. Generalized linear models were used to test for the influence of species, δ(15)N, and δ(13)C on breast feather Hg. The most parsimonious model included species, δ(15)N, and their interaction term and explained 36% of the variation in breast feather Hg. Each species exhibited a different association between breast feather δ(15)N and Hg with Seaside Sparrows showing a positive correlation (r=0.27, P=0.03), Nelson's Sparrows a negative correlation (r=-0.28, P=0.01), and Saltmarsh Sparrows with no significant association. For Saltmarsh Sparrows, δ(15)N and Hg revealed decoupling between breast feather Hg and trophic position. Our results demonstrate that the influence of δ(15)N on breast feather Hg is likely indicative of geographic variation in δ(15)N baselines rather than trophic position.