Raman spectroscopic investigations of sarcoplasmic reticulum membranes.

Raman spectra are presented for sarcoplasmic reticulum membranes. Interpretation of the 1000-1130 cm-1 region of the spectrum indicates that the sarcoplasmic reticulum membrane may be more fluid than erythrocyte membranes that have been examined by the I portion of the membrane spectrum with a strong 1658 cm-1 band characteristic of C=C stretching in hydrocarbon side chains exhibiting cis conformation. This band is unaltered in intensity and position in H2O and in 2H2O thus obscuring amide I protein conformation. Of particular interest is the appearance of strong, resonantly enhanced bands at 1160 and 1527 cm-1 attributable to membrane-associated carotenoids.

A fiber-optic sensor for CO(2) measurement.

A fiber-optic sensor has been prepared which responds to carbon dioxide at physiologically significant concentrations. It is based on pH modulation by dissolved carbon dioxide in a sensing layer of fluorescent dye. By use of a previously developed methodology by which the sensing chemistry is bonded directly to the glass fiber tip, the miniature size of the sensor is preserved. This method involves consecutive applications of solution polymers to the fiber tip rather than mechanical attachment of sensor reagents. Preparations of polymer-immobilized dyes and polymer membranes are described.

A fiber-optic sensor system for monitoring chlorinated hydrocarbon pollutants.

We have developed and field-tested a fiber-optic chemical sensor system for use in environmental monitoring and remediation. The system detects chlorinated hydrocarbon pollutants with colorimetry, and is based on an irreversible chemical reaction between the target compound and a specific reagent. The reaction products are detected by their absorption at 560 nm and can be monitored remotely with optical fibers. Continuous measurements are made possible by renewing the reagent from a reservoir with a miniature pumping system. The sensor has been evaluated against gas chromatography standards and has demonstrated accuracy and sensitivity (5 ppbw) sufficient for the environmental monitoring of trichloroethylene and chloroform. Successful preliminary field tests have been conducted in a variety of contamination monitoring scenarios.

Evidence of novel secondary structure in DNA-bound protamine is revealed by Raman spectroscopy.

Raman spectroscopy studies of protamine-DNA complexes are reported for samples in the solid state at 98% relative humidity. Previous reports utilizing other physical techniques have indicated the presence of B-form DNA in protamine-DNA complexes. The present Raman data support the assignment of a modified B-form which is characterized by appreciable unstacking of the bases. The quality of the present spectra has made it possible, for the first time, to obtain the Raman spectrum of DNA-bound protamine by digital spectral subtraction. The difference spectrum indicates that protamine adopts an unusual secondary structure upon binding to DNA. A dominant amide I band is observed at 1683 cm-1 which is indicative of neither an alpha-helix or beta-sheet conformation. An amide I band at this position has been associated with the 1-->3 hydrogen bond that occurs within a gamma-turn [Bandekar, J., & Krimm, S. (1985) Int. J. Pept. Protein Res. 26, 158-165]. On the basis of this assignment, as well as preliminary results obtained by computer modeling, we propose a new model for the secondary structure of DNA-bound protamine that is rich in 1-->3 hydrogen bonding. Spectral data demonstrate that this structure is absent in protamine molecules in solution. Analyses of spectra of polyarginine-DNA complexes suggest that polyarginine, although similar to protamine in primary structure, assumes a conformation when bound to DNA that is distinct from that adopted by protamine.

Dissolved yellow organics: quantitative and qualitative aspects of extraction by four common techniques.

The dissolved yellow organic matter is removed from aliquots of the same freshwater sample by four common techniques: lyophilization, ion-exchange, ultrafiltration and organic solvent. Quantitative and qualitative aspects of the recovery, including fractionation by gel filtration and total nitrogen determinations, are presented.

Field-deployable sniffer for 2,4-dinitrotoluene detection.

A field-deployable instrument has been developed to detect low-level 2,4-dinitrotoluene (2,4-DNT) vapors. The system is based on previously developed artificial nose technology and employs an array of sensory materials attached to the distal tips of an optical fiber bundle. Both semiselective and nonspecific, cross-reactive sensors were employed. Each sensor within the array responds differentially to vapor exposure so the array's fluorescence response patterns are unique for each analyte. The instrument is computationally "trained" to discriminate target response patterns from nontarget and background environments. This detection system has been applied to detect 2,4-DNT, an analyte commonly detected on the soil surface above buried 2,4,6-trinitrotoluene (TNT) land mines, in spiked soil and aqueous and ground samples. The system has been characterized and demonstrated the ability to detect 120 ppb 2,4-DNT vapor in blind (unknown) humidified samples during a supervised field test.