[Advances in researches on mechanism of macrophage migration inhibitory factor regulating parasite-host immune interaction].

Macrophage migration inhibitory factor (MIF), a type of pleiotropic immunoregulatory cytokine with a specific structure, participates in the regulation of host cell growth and migration and immune responses. Following parasitic infections, hosts may produce MIF and then participate in the parasite-host interactions. In addition, parasites may secrete parasite-derived MIF, and they jointly participate in parasite-host interactions. This paper reviews the regulation of MIF gene expression following parasitic infections, the role of MIF in parasite-host immune system interactions, and important signaling pathways of MIF-mediated immune responses.

[Human thelaziasis callipaeda in Tongren area of Guizhou Province: a case report].

This paper reports a case of human thelaziasis callipaeda in Tongren area of Guizhou Province.

[Sequential analysis of genome of Thelazia callipaeda].

OBJECTIVE: To investigate the molecular characteristics of genome sequence of Thelazia callipaeda (T. cp). METHODS: The obtained T. cp genome assembling data were annotated by using a combination of ab initio gene by softwares, GeneMark and GeneID, and the homology of the experimentally confirmed genes was predicted by software GeMoMa. The results were integrated by software EVM to predict all genes of genome. The obtained genes were annotated in the common public database and three dedicated databases (CAZyme, TCDB and PHI), respectively. RESULTS: The Scaffolds and Contigs gene structure of T. cp genome (79.34 Mb) was analyzed, and a total of 6 333 genes were obtained. The sequence search was conducted in the public databases using BLASTx, of which 97.85% of the genes could be annotated. The genes annotated in the NR database were the most (98.69%), and those enriched in the KEGG pathway were the least (50.50%). The functional genes were blasted by KOG database and totally 4 517 genes were found. The three special databases (CAZyme, TCDB and PHI) were used to annotate all the genes, and 136, 139 and 1 498 genes were assigned respectively, and the number of genes in the PHI database was the largest. In the cytochrome proprietary database, 238 cytochrome P450 genes were predicted. CONCLUSIONS: We have preliminarily revealed the T. cp genome structure characteristics and annotation information, and totally 6 333 genes are obtained.

[Genetic variation of Thelazia callipaeda among isolates collected from patients in Zunyi City, Guizhou Province].

OBJECTIVE: To identify the genetic variation and possible sources of Thelazia callipaeda isolates collected from patients in Zunyi City, Guizhou Province. METHODS: Seven cases of T. callipaeda infection in Zunyi City, 2016 were verified, and DNA (s) were extracted from the T. callipaeda's body collected from the thelaziasis patients. A mitochondrial COX1 fragment was amplified and sequenced. The sequence alignment and phylogenetical analysis were performed to compare the genetic variation of the gene sequence with the homologous sequences downloaded from Genebank. RESULTS: COX1 genes of T. callipaeda were differed among the samples from the seven cases, which had low variation. CONCLUSIONS: Zunyi City is a new area with endemic of thelaziasis. The isolates from Zunyi City include either Asian origin or European origin of T. callipaeda. Moreover, at least four haplotypes are identified among the seven isolates.