1.
Angew Chem Int Ed Engl
; 59(14): 5505-5509, 2020 03 27.
Artículo
en Inglés
| MEDLINE
| ID: mdl-31895495
RESUMEN
1-Aminopyrenes with three ω-hydroxylated N-alkylsulfonamido or alkylsulfonyl residues in positions 3, 6, and 8 were prepared, O-phosphorylated, and applied for reductive amination of oligosaccharides. The dyes (ϵ≈20 000 m-1 cm-1 ) with six negative charges (pH≥8) and low m/z ratios enable labeling and fluorescence detection of reducing sugars (glycans) related to the most structurally and functionally diverse class of natural products. Under excitation with a 488â nm laser, the new glycoconjugates emit yellow light of about 560â nm, outperforming (with respect to brightness and faster electrophoretic mobilities) the corresponding APTS derivatives (benchmark dye with green emission in conjugates).