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1.
J Am Chem Soc ; 146(5): 2919-2927, 2024 Feb 07.
Artículo en Inglés | MEDLINE | ID: mdl-38277794

RESUMEN

Dicarboxylic acids and derivatives are important building blocks in organic synthesis, biochemistry, and the polymer industry. Although catalytic dicarboxylation with CO2 represents a straightforward and sustainable route to dicarboxylic acids, it is still highly challenging and limited to generation of achiral or racemic dicarboxylic acids. To date, catalytic asymmetric dicarboxylation with CO2 to give chiral dicarboxylic acids has not been reported. Herein, we report the first asymmetric dicarboxylation of 1,3-dienes with CO2 via Cu catalysis. This strategy provides an efficient and environmentally benign route to chiral dicarboxylic acids with high regio-, chemo-, and enantioselectivities. The copper self-relay catalysis, that is, Cu-catalyzed boracarboxylation of 1,3-dienes to give carboxylated allyl boronic ester intermediates and subsequent carboxylation of C-B bonds to give dicarboxylates, is key to the success of this dicarboxylation. Moreover, this protocol exhibits broad substrate scope, good functional group tolerance, easy product derivatizations, and facile synthesis of chiral liquid crystalline polyester and drug-like scaffolds.

2.
J Clin Lab Anal ; 33(2): e22692, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30320481

RESUMEN

BACKGROUND: Long noncoding RNAs (lncRNAs) have roles in regulating metabolism; however, the global expression profile of metabolic pathway-associated lncRNAs in gastric cancer is unknown. The purpose of our study was to examine metabolic pathway-related lncRNAs in gastric cancer and their possible diagnostic values. METHODS: Differential expression patterns of metabolic pathway-related lncRNAs between gastric cancer and paired nontumor tissues were detected using metabolic pathway-associated lncRNA microarrays. The expression of RP11-555H23.1, one representative metabolic pathway-associated lncRNA, was validated using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). The associations between RP11-55H23.1 expression and the clinicopathological features of gastric cancer patients were analyzed. A receiver operating characteristic (ROC) curve was further established. RESULTS: A total of 114 differentially expressed metabolic pathway-associated lncRNAs (fold change >2, P < 0.05) between cancer and nontumor tissues were found (GEO No. GSE96856). Among them, TUG1, RP11-555H23.1, RP1-257I20.13, UGP2, GCSHP3, and XLOC_000889 lncRNAs were downregulated more than sixfold in gastric cancer tissues. In contrast, RP11-605F14.2, TBC1D3P5, BC130595, LINC00475, RP11-19P22.6, BC080653, XLOC_004923, AFAP1-AS1, EPB49, and RP11-296I10.3 lncRNAs were upregulated more than sixfold in gastric cancer tissues. We further demonstrated that RP11-555H23.1 expression was significantly correlated with TNM stage (P = 0.038). The area under the ROC curve (AUC) was 0.65, and the specificity and sensitivity were 62% and 81%, respectively. CONCLUSIONS: Metabolic pathway-associated lncRNAs play an important role in the occurrence of gastric cancer, and metabolic pathway-associated lncRNAs, such as RP11-555H23.1, may represent novel biomarkers of gastric cancer.


Asunto(s)
Biomarcadores de Tumor/genética , ARN Largo no Codificante/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/metabolismo , Transcriptoma/genética , Biomarcadores de Tumor/análisis , Biomarcadores de Tumor/metabolismo , Femenino , Perfilación de la Expresión Génica/métodos , Regulación Neoplásica de la Expresión Génica/genética , Humanos , Masculino , Redes y Vías Metabólicas/genética , Persona de Mediana Edad , ARN Largo no Codificante/análisis , ARN Largo no Codificante/metabolismo , Curva ROC
3.
Br J Cancer ; 116(5): 626-633, 2017 Feb 28.
Artículo en Inglés | MEDLINE | ID: mdl-28081541

RESUMEN

BACKGROUND: Circular RNAs (circRNAs) are a class of non-coding RNAs broadly expressed in cells of various species. Their role in cancers, especially in gastric cancer, is poorly understood. METHODS: Circular RNA 0000096 (hsa_circ_0000096) levels in 101 paired gastric cancer tissues and adjacent non-tumorous tissues from patients with gastric cancer were detected by real-time quantitative reverse transcription-polymerase chain reaction. A receiver operating characteristic curve was generated to evaluate the diagnostic value of hsa_circ_0000096. RNA interference was used to manipulate the expression of hsa_circ_0000096. Its biological effects were evaluated by flow cytometry, real-time cell analysis, a wound scratch assay, western blot analysis and xenograft models. RESULTS: Hsa_circ_0000096 was found to be significantly downregulated in gastric cancer tissues and gastric cancer cell lines compared with paired adjacent non-tumorous tissues and normal gastric epithelial cells (P<0.001). Moreover, knockdown of hsa_circ_0000096 significantly inhibited cell proliferation and migration in vitro and in vivo. The results of both immunohistochemical and western blot analyses showed that the protein levels of cyclin D1, cyclin-dependent kinase 6 (CDK6), matrix metalloproteinase-2 and MMP-9 were significantly reduced in vitro and in vivo. A gastric cancer xenograft nude mouse model indicated that Ki67 and VEGF were reduced in a dose-dependent manner following knockdown of hsa_circ_0000096. However, the expression of E-cadherin increased. CONCLUSIONS: Hsa_circ_0000096 may be used as a potential novel biomarker for gastric cancer. It affects gastric cancer cell growth and migration by regulating cyclin D1, CDK6, MMP-2 and MMP-9.


Asunto(s)
Regulación hacia Abajo , ARN/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patología , Animales , Proteínas de Ciclo Celular/metabolismo , Línea Celular Tumoral , Movimiento Celular , Proliferación Celular , Regulación Neoplásica de la Expresión Génica , Humanos , Ratones , Trasplante de Neoplasias , Valor Predictivo de las Pruebas , ARN Circular , Curva ROC
4.
Br J Nutr ; 115(9): 1547-55, 2016 05.
Artículo en Inglés | MEDLINE | ID: mdl-26948765

RESUMEN

The c-Jun N-terminal kinases (JNK) are members of the mitogen-activated protein kinase family and are activated by environmental stress. Se plays an important role in the biological pathways by forming selenoprotein. Selenoproteins have been shown to exhibit a variety of biological functions including antioxidant functions and maintaining cellular redox balance, and compromise of such important proteins would lead to oxidative stress and apoptosis. We examined the expression levels of JNK in Kashin-Beck disease (KBD) patients, tested the potential protective effects of sodium selenite on tert-butyl hydroperoxide (tBHP)-induced oxidative injury and apoptosis in human chondrocytes as well as its underlying mechanism in this study. We produced an oxidative damage model induced by tBHP in C28/I2 human chondrocytes to test the essential anti-apoptosis effects of Se in vitro. The results indicated that the expression level of phosphorylated JNK was significantly increased in KBD patients. Cell apoptosis was increased and molecule expressions of the JNK signalling pathway were activated in the tBHP-injured chondrocytes. Na2SeO3 protected against tBHP-induced oxidative stress and apoptosis in cells by increasing cell viability, reducing reactive oxygen species generation, increasing Glutathione peroxidase (GPx) activity and down-regulating the JNK pathway. These results demonstrate that apoptosis induced by tBHP in chondrocytes might be mediated via up-regulation of the JNK pathway; Na2SeO3 has an effect of anti-apoptosis by down-regulating the JNK signalling pathway.


Asunto(s)
Condrocitos/efectos de los fármacos , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Enfermedad de Kashin-Beck/metabolismo , Sistema de Señalización de MAP Quinasas , Osteoartritis/metabolismo , Estrés Oxidativo/efectos de los fármacos , Selenito de Sodio/farmacología , Antioxidantes/metabolismo , Antioxidantes/farmacología , Apoptosis/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Condrocitos/metabolismo , Regulación hacia Abajo , Glutatión Peroxidasa/metabolismo , Humanos , Fosforilación , Especies Reactivas de Oxígeno/metabolismo , Selenio/farmacología , Transducción de Señal , Regulación hacia Arriba , terc-Butilhidroperóxido
5.
Heliyon ; 10(7): e28386, 2024 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-38560250

RESUMEN

Background: Immune escape remains a major challenge in the treatment of malignant tumors. Here, we studied the mechanisms underlying immune escape in the tumor microenvironment and identified a potential therapeutic target. Methods: Pathological specimens from patients with liver cancer, soft tissue sarcoma, and liver metastasis of colon cancer were subjected to immunohistochemistry analysis to detect the expression of programmed death-1 (PD-1) in the tumor microenvironment (TME). Additionally, the expression of regulatory T cells (Tregs) and long non-coding RNAs (lncRNAs), such as highly upregulated in liver cancer (HULC) was evaluated by fluorescence in situ hybridization, and the relationship between HULC, Treg cells, and PD-1 was determined. The animals were divided into H22 hepatic carcinoma and S180 sarcoma groups. Each group was divided into Foxp3-/-C57BL/6J and C57BL/6J mice. Thereafter, mice were inoculated with 0.1 ml S180 sarcoma cells or 0.1 ml H22 hepatoma cells, at a concentration of 1 × 107/ml. The number of splenic CD4+CD25+Foxp3+ T cells was detected by flow cytometry, and serum interleukin-10 (IL-10) and transforming growth factor ß1 (TGF-ß1) levels were detected using a Luminex liquid suspension chip. Expression of PD-1, fork head box P3 (Foxp3), and HULC in the TME, were analyzed and the therapeutic effect of inhibiting the lncRNA HULC-Treg-PD-1 axis in malignant tumors was determined. Results: High expression of lncRNA HULC promotes the proliferation of Treg cells and increases PD-1 expression in the tumor microenvironment. The HULC-Treg-PD-1 axis plays an immunosuppressive role and promotes the proliferation of malignant tumors. Knocking out the Foxp3 gene can affect the HULC-Treg-PD-1 axis and reduce PD-1, IL-10, and TGF-ß1 expression to control the growth of malignant tumors. Conclusion: The lncRNA HULC-Treg-PD-1 axis promotes the growth of malignant tumors. This axis could be modulated to reduce PD-1, IL-10, and TGF-ß1 expression and the subsequent immune escape. The inhibition of immune escape in the tumor microenvironment can be achieved by controlling the LncRNA HULC-Treg-PD-1 axis.

6.
PLoS One ; 19(3): e0298271, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38502664

RESUMEN

Multiple Sclerosis (MS) is an immune-related disease and the relationship between MS and cancer has raised attention. Previous studies of the relationship between MS and cancer have reached conflicting conclusions. In this study, the two-sample MR method is used to investigate whether MS has a causal correlation with cancers and offer scientific evidence for cancer prevention. Single nucleotide polymorphisms (SNPs) related to MS were obtained from the genome-wide association study (GWAS) based on International Multiple Sclerosis Genetics Consortium (IMSGC) and SNPs related to 15 types of cancers were obtained from the GWASs based on UK Biobank. Inverse variance weighted (IVW) method was mainly used to assess causal effects. Sensitivity analyses were conducted with Cochran's Q-test, MR Egger intercept, leave-one-out test, and MR Steiger method. IVW analysis showed that MS was only associated with a marginal increased risk of cervical cancer (OR 1.0004, 95% CI 1.0002-1.0007, p = 0.0003). Sensitivity analyses showed that the results of MR analysis were robust and found no heterogeneity, no pleiotropy, and no reverse causation. In conclusion, this study finds no causal relationship between MS and 15 types of cancers except cervical cancer.


Asunto(s)
Esclerosis Múltiple , Neoplasias del Cuello Uterino , Femenino , Humanos , Estudio de Asociación del Genoma Completo , Análisis de la Aleatorización Mendeliana , Esclerosis Múltiple/epidemiología , Esclerosis Múltiple/genética , Nonoxinol
7.
Food Chem X ; 14: 100342, 2022 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-35637757

RESUMEN

In present work, Zanthoxylum bungeanum meal (ZBM) used as experimental material, the stability of typical alkylamides (hydroxyl-α-sanshool and hydroxyl-ß-sanshool) in ZBM under different acidification conditions was investigated, in order to reveal degradation or transformation mechanism of numbing substances from Z. bungeanum exposed to acid environment and its transform direction. The alkylamides content of ZBM was detected by using HPLC after different conditions of acidification. The results indicated that hydroxyl-α-sanshool and hydroxyl-ß-sanshool under the concentration of hydrochloric acid is 14% decreased by 80% after only 0.5 h. Moreover, some of the components undergo isomerization and addition reactions in the process of acidification, the products of isomerization are hydroxyl-ε-sanshool and (1Z,2E,4E,8E,10E)-N-(2-hydroxy-2-methylpropyl)dodeca-2,4,8,10-tetraenimidic acid; and the product of the addition reaction is (2E,6E,8E,10E)-1-chloro-1-(2-hydroxy-2-methylpropyl)amino)dodeca-2,6,8,10-tetraen-1-ol, which indicated that acid environment has greatly changed the numbing substances in Z. bungeanum and its products.

8.
Mol Biol Rep ; 38(2): 793-9, 2011 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-20383585

RESUMEN

In order to study the impalpable effect of GFP in homozygous heart-specific GFP-positive zebrafish during the early stage, the researchers analyzed the heart function of morphology and physiology at the first 3 days after fertilization. This zebrafish line was produced by a large-scale Tol2 transposon mediated enhancer trap screen that generated a transgenic zebrafish with a heart-specific expression of green fluorescent protein (GFP)-tagged under control of the nppa enhancer. In situ hybridization experiments showed that the nppa:GFP line faithfully recapitulated both the spatial and temporal expressions of the endogenous nppa. Green fluorescence was intensively and specifically expressed in the myocardial cells located both in the heart chambers and in the atrioventricular canal. The embryonic heart of nppa:GFP line developed normally compared with those in the wild type. There was no difference between the nappa:GFP and wild type lines with respect to heart rate, overall size, ejection volume, and fractional shortening. Thus the excess expression of GFP in this transgenic line seemed to exert no detrimental effects on zebrafish hearts during the early stages.


Asunto(s)
Factor Natriurético Atrial/química , Factor Natriurético Atrial/genética , Proteínas Fluorescentes Verdes/biosíntesis , Corazón/embriología , Miocardio/metabolismo , Animales , Animales Modificados Genéticamente , Elementos de Facilitación Genéticos , Proteínas Fluorescentes Verdes/química , Corazón/fisiología , Hibridación in Situ , Modelos Genéticos , Fenotipo , ARN Mensajero/metabolismo , Transgenes , Pez Cebra
9.
Neurochem Res ; 35(1): 33-41, 2010 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-19578991

RESUMEN

Astragalus mongholicus (AM) is a traditional medicinal herb used as a neuroprotective agent for its anxiolytic, antidepressant, antiamnestic, and antiaggresive effects. However, the mechanisms underlying its anti-convulsant properties are not well studied. In the present study, we examined the anticonvulsant effects on pentylenetetrazol (PTZ)-induced seizures in mice and the possible mechanisms of protection against oxidative damage and mitochondrial dysfunction in vitro. The behavioral studies showed that the root extract of AM had powerful anticonvulsant effects against seizures induced by PTZ and the biochemical studies showed that root extract of AM inhibited PTZ-induced increase in lipid peroxidation, protein oxidation and reactive oxygen species, and enhanced mitochondrial function. Electron spin resonance spectroscopy studies demonstrated that the extracts from the root and aerial parts of AM possess potent effects on scavenging hydroxyl and lipid free radicals. We found that AM extract significantly protected malondialdehyde-induced oxidative damage by ameliorating activities of the mitochondrial complexes I, II, malate dehydrogenase and mitochondrial membrane potential. These data suggest that the anti-convulsant effects of AM extract may be mediated by its protective actions against oxidative damage and amelioration of mitochondrial dysfunction.


Asunto(s)
Anticonvulsivantes/farmacología , Planta del Astrágalo/química , Depuradores de Radicales Libres/farmacología , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Encéfalo/efectos de los fármacos , Estimulación Eléctrica , Espectroscopía de Resonancia por Spin del Electrón , Técnicas In Vitro , Masculino , Ratones , Ratones Endogámicos BALB C , Mitocondrias/efectos de los fármacos , Pentilenotetrazol/toxicidad , Ratas , Ratas Sprague-Dawley , Convulsiones/etiología , Convulsiones/prevención & control
10.
Biol Trace Elem Res ; 190(2): 303-308, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-30474788

RESUMEN

Kaschin-Beck disease (KBD) is an endemic, chronic, and degenerative osteoarthropathy, which seriously impairs the quality of patients' life. We detected the expression of TrxR by ELISA and found that TrxR was lower in KBD than in normal control group significantly (P < 0.001); this result indicated that TrxR must be related to KBD. We retrieved cSNPs in NCBI SNP database and used three bioinformatics programmers, including SIFT, PolyPhen, and SNP3d, to help select the researched nsSNP. Then, we used PCR-RFLP to analyze the relationship between the SNP site rs5746841 in TrxR2 gene and susceptibility of KBD and detected the expression of Nrf2 and HO-1 by western blot. The results showed that the genotype of rs5746841 in 93 normal controls and 103 KBD subjects were C/C totally, but A/A and A/C were not found, which indicated preliminarily that there was no correlation between rs5746841 in TrxR2 gene and susceptibility of KBD. The expression of TrxR was lower in KBD than in normal control group significantly, while the expressions of Nrf2 and HO-1 were higher in KBD than in normal control group. These results indicated that the low expression of selenoprotein TrxR may be a candidate factor of KBD, which related to Nrf2/HO-1 signaling pathway.


Asunto(s)
Hemo-Oxigenasa 1/genética , Enfermedad de Kashin-Beck/genética , Factor 2 Relacionado con NF-E2/genética , Polimorfismo Genético/genética , Transducción de Señal/genética , Reductasa de Tiorredoxina-Disulfuro/genética , Ensayo de Inmunoadsorción Enzimática , Femenino , Genotipo , Humanos , Enfermedad de Kashin-Beck/metabolismo , Masculino , Persona de Mediana Edad , Reductasa de Tiorredoxina-Disulfuro/metabolismo
11.
Bone ; 120: 239-245, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-29653292

RESUMEN

The aim of the study was to investigate the association between rs5859 in Sep15, rs1139793 in TrxR2 polymorphisms with the risks of KBD and to detect the expression of AP-1 pathway in KBD subjects and in vitro. 208 KBD and 206 control subjects were included. PCR-Restriction Fragment Length Polymorphism (RFLP), Amplification Refractory Mutation Specific-PCR (ARMS-PCR) and Western Blotting were conducted. The results showed the minor A-allele frequency of rs5859 in KBD was statistically significantly higher than that in the control group (P < 0.05). The cases carrying A-allele had a 2-fold (95%CI: 1.064-3.956) increased risk of developing KBD compared with the G-allele carriers. There was no significant difference in genotype and allele distribution of rs1139793 between KBD patients and controls (P > 0.05). The frequency of the minor A allele of rs5859 was significantly different in Chinese healthy population compared with European, African and American. The frequency of the minor A allele of rs1139793 showed significant difference when compared with African and American. The levels of JunB, JunD, P65 proteins in KBD group were higher than those in control group (P < 0.0001). The expression of JunB, JunD, P65 proteins all increased in tBHP-induced C28/I2 oxidative damage model compared with control group (P < 0.05) and decreased after Se supplementation. Our finding indicated Sep15 is a possible candidate susceptibility gene for KBD. Combined with the in vitro study, our studies reveal novel insights into the mechanism of Se supplementation as an antioxidant via inhibiting the AP-1 signaling pathway in patients with KBD.


Asunto(s)
Predisposición Genética a la Enfermedad , Enfermedad de Kashin-Beck/genética , Polimorfismo de Nucleótido Simple/genética , Selenoproteínas/genética , Transducción de Señal , Tiorredoxina Reductasa 2/genética , Factor de Transcripción AP-1/metabolismo , Apoptosis/efectos de los fármacos , Estudios de Casos y Controles , Línea Celular , Condrocitos/efectos de los fármacos , Condrocitos/metabolismo , Etnicidad/genética , Femenino , Frecuencia de los Genes , Humanos , Masculino , Persona de Mediana Edad , Selenio/farmacología
12.
Mol Genet Genomic Med ; 6(5): 728-738, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-29992774

RESUMEN

BACKGROUND: Long noncoding RNAs (lncRNAs) play important roles in carcinogenesis. However, the roles of metabolism-associated lncRNAs in cancers are still unclear. METHODS: A microarray of metabolism-associated lncRNAs was used to detect their expression patterns between gastric cancer and paired nontumorous tissues. Its results and gastric cancer differential gene expression data from public databases were used to screen the metabolic pathway-associated lncRNAs. A metabolic network with microRNAs (miRNAs), lncRNAs, and protein-coding genes was further constructed. Finally, the expression of TOPORS antisense RNA 1 (TOPORS-AS1), a screened highly expressed lncRNA and its associated protein-coding gene, NADH: ubiquinone oxidoreductase subunit B6 (NDUFB6), were verified by reverse transcription polymerase chain reaction. RESULTS: A total of eight upregulated and one downregulated lncRNAs and 25 upregulated and 20 downregulated protein-coding genes were found to be involved in metabolism in gastric cancer. Within the lncRNAs-miRNAs-mRNAs metabolic network, 78 miRNA-target links, 546 positive coexpression relationships, and 191 protein-protein interactions were found. The expression of TOPORS-AS1 and its associated gene, NDUFB6 in gastric cancer tissues was significantly lower than that in adjacent nontumor tissues. Moreover, NDUFB6 expression was associated with the invasion and distal metastasis of gastric cancer. CONCLUSIONS: The metabolism-associated lncRNAs play important roles in the occurrence of gastric cancer.


Asunto(s)
Regulación Neoplásica de la Expresión Génica , Redes Reguladoras de Genes , Proteínas de Neoplasias , ARN Largo no Codificante , ARN Neoplásico , Neoplasias Gástricas , Femenino , Humanos , Masculino , Invasividad Neoplásica , Metástasis de la Neoplasia , Proteínas de Neoplasias/biosíntesis , Proteínas de Neoplasias/genética , ARN Largo no Codificante/biosíntesis , ARN Largo no Codificante/genética , ARN Neoplásico/biosíntesis , ARN Neoplásico/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patología
13.
Oncotarget ; 8(35): 58405-58416, 2017 Aug 29.
Artículo en Inglés | MEDLINE | ID: mdl-28938566

RESUMEN

Circular RNAs (circRNAs) have been emerged as an indispensable part of endogenous RNA network. However, the expression significance of circRNAs in hepatocellular carcinoma (HCC) is rarely revealed. The aim of this study was to determine the circRNA expression profile in HCC, and to investigate their clinical significances and relevant mechanisms for cancer progression. The global circRNA expression profile in HCC was measured by circRNA microarray. Levels of one representative circRNAs, hsa_circ_0004018, were confirmed by real-time reverse transcription-polymerase chain reaction. The expression levels of hsa_circ_0004018 in HCC were significantly lower compared with para-tumorous tissue (P<0.001). Our data further showed that lower expression of hsa_circ_0004018 was correlated with serum alpha-fetoprotein (AFP) level, tumor diameters, differentiation, Barcelona Clinic Liver Cancer stage and Tumor-node-metastasis stage. More importantly, we detected liver tissues from chronic hepatitis, cirrhosis and HCC patients; and found that hsa_circ_0004018 harbored HCC-stage-specific expression features in diverse chronic liver diseases (P<0.001). The area under receiver operating characteristic curve was up to 0.848 (95% CI=0.803-0.894, P<0.001). The sensitivity and specificity were 0.716 and 0.815, respectively. Finally, hsa_circ_0004018 might be involved in cancer-related pathways via interactions with miRNAs.

14.
Cancer Biomark ; 18(4): 397-403, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28128738

RESUMEN

BACKGROUND: Long non-coding RNAs (lncRNAs) play roles in carcinogenesis; however, the significance of most lncRNAs in gastric cancer is unclear. OBJECTIVE: To explore the diagnostic value of the long noncoding RNA RP11-19P22.6-001 in gastric cancer. METHODS: RP11-19P22.6-001 levels in gastric cancer tissues and paired non-tumor tissues were analyzed by quantitative reverse transcription-polymerase chain reaction. Since RP11-19P22.6-001 acts in cis to regulate nitric oxide synthase 2 (NOS2), we also analyzed NOS2 expression and its correlation with gastric cancer. Finally, to analyze the potential diagnostic values of RP11-19P22.6-001, a receiver operating characteristic (ROC) curve was constructed. RESULTS: RP11-19P22.6-001 expression was significantly downregulated in 70.91% (78/110) of gastric cancer tissues compared to that of adjacent normal tissues. However, NOS2 was upregulated in 75.45% (83/110) of gastric cancer tissues. RP11-19P22.6-001 expression levels were associated with invasion, lymph node metastasis, and TNM stage. The areas under the ROC curves (AUC) were 0.662 and 0.671 for RP11-19P22.6-001 and NOS2, respectively. More importantly, the combined use of these parameters increased the AUC to 0.704. CONCLUSIONS: RP11-19P22.6-001 and NOS2 may be new biomarkers for the diagnosis and prognosis of gastric cancer. The combined use of lncRNAs and their target genes may be a promising method to increase the diagnostic value of lncRNAs in cancer.


Asunto(s)
Biomarcadores de Tumor/genética , Óxido Nítrico Sintasa de Tipo II/genética , ARN Largo no Codificante/genética , Neoplasias Gástricas/genética , Anciano , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Metástasis Linfática , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/patología
15.
Sheng Wu Gong Cheng Xue Bao ; 32(11): 1507-1518, 2016 Nov 25.
Artículo en Zh | MEDLINE | ID: mdl-29034621

RESUMEN

Recently, with the development of RNA research techniques, a wide variety of circular RNAs (circRNAs) have been discovered and some of them are confirmed to have crucial biological functions. CircRNAs arise from exons (i.e. exonic circRNAs) or introns (i.e. intronic circRNAs). Acting as microRNA sponges or combining with proteins, circRNAs participate in the regulation of gene expression and influence the activity of some proteins. In addition, some circRNAs even encode proteins. More importantly, several circRNAs play a key role in the occurrence and progression of some tumors, including stomach, liver, colon, breast, cervical, and ovarian cancers. Therefore, circRNAs may be a novel type of diagnostic marker and therapeutic target of cancers.


Asunto(s)
Carcinogénesis/genética , Neoplasias/genética , ARN/genética , Regulación Neoplásica de la Expresión Génica , Humanos , MicroARNs/genética , ARN Circular
16.
Oncotarget ; 7(8): 8601-12, 2016 Feb 23.
Artículo en Inglés | MEDLINE | ID: mdl-26788991

RESUMEN

Long noncoding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides. Aberrant expression of lncRNAs has been found associated with gastric cancer, one of the most malignant tumors. By complementary base pairing with mRNAs or forming complexes with RNA binding proteins (RBPs), some lncRNAs including GHET1, MALAT1, and TINCR may mediate mRNA stability and splicing. Other lncRNAs, such as BC032469, GAPLINC, and HOTAIR, participate in the competing endogenous RNA (ceRNA) network. Under certain circumstances, ANRIL, GACAT3, H19, MEG3, and TUSC7 exhibit their biological roles by associating with microRNAs (miRNAs). By recruiting histone-modifying complexes, ANRIL, FENDRR, H19, HOTAIR, MALAT1, and PVT1 may inhibit the transcription of target genes in cis or trans. Through these mechanisms, lncRNAs form RNA-dsDNA triplex. CCAT1, GAPLINC, GAS5, H19, MEG3, and TUSC7 play oncogenic or tumor suppressor roles by correlated with tumor suppressor P53 or onco-protein c-Myc, respectively. In conclusion, interaction with DNA, RNA and proteins is involved in lncRNAs' participation in gastric tumorigenesis and development.


Asunto(s)
Regulación Neoplásica de la Expresión Génica , ARN Largo no Codificante/genética , Neoplasias Gástricas/genética , Animales , Humanos
17.
Clin Chim Acta ; 444: 132-6, 2015 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-25689795

RESUMEN

BACKGROUND: Circular RNAs (circRNAs), a class of endogenous RNAs, have emerged as an enigmatic class of RNAs. Little is known about their value in the diagnosis of cancers. METHODS: The targeted circRNA of this study was selected using two circRNA databases: CircBase (http://circbase.org/) and circ2Traits (http://gyanxet-beta.com/circdb/). Divergent primers, rather than commonly used convergent primers, for the circRNA were designed. The circRNA levels in 101 paired gastric cancer tissues and adjacent nontumorous tissues from surgical gastric cancer patients and 36 paired plasma samples from preoperative and postoperative gastric cancer patients were analyzed by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The specificity of the amplified products was measured by melting curve analysis and DNA sequencing. To observe the stability of circRNA, three randomly selected samples of gastric cancer tissues were stored at room temperature, 4°C and -20°C, and then, their circRNA levels were analyzed. To verify the reproducibility of qRT-PCR, circRNA levels were detected in a set of specimens (n=15) in two independent experiments with an interval of one day. Then, the correlation of their Ct values was determined. The relationships between circRNA expression levels and clinicopathological factors of patients with gastric cancer were further analyzed by one-way analysis of variance. A receiver operating characteristic (ROC) curve was established to evaluate the diagnostic value. RESULTS: Hsa_circ_002059, a typical circular RNA, was first found to be significantly downregulated in gastric cancer tissues compared with paired adjacent nontumorous tissues (p<0.001). Its levels in plasma collected from postoperative gastric cancer patients were found significantly different from those from preoperative gastric cancer patients. The area under the ROC curve was 0.73. Importantly, we further found that lower expression levels were significantly correlated with distal metastasis (P=0.036), TNM stage (P=0.042), gender (P=0.002) and age (P=0.022). The stability of circRNAs and the reproducibility of the qRT-PCR method for detecting circRNA levels were determined. CONCLUSION: These results suggested that circRNAs are highly stable in mammalian cells and that one specific circRNA, hsa_circ_002059, may be a potential novel and stable biomarker for the diagnosis of gastric carcinoma.


Asunto(s)
ARN/aislamiento & purificación , Neoplasias Gástricas/diagnóstico , Biomarcadores/análisis , Femenino , Humanos , Masculino , Persona de Mediana Edad , ARN/genética , ARN Circular , Reacción en Cadena en Tiempo Real de la Polimerasa , Neoplasias Gástricas/química , Neoplasias Gástricas/genética
18.
Wei Sheng Wu Xue Bao ; 43(6): 809-12, 2003 Dec.
Artículo en Zh | MEDLINE | ID: mdl-16276907

RESUMEN

Aeromonas hydrophila WQ isolated from lake water was found to be able to synthesize polyhydroxyalkanoates (PHA) copolymer consisting of 3-hydroxybutyrate (HB) and 3-hydroxyhexanoate (HHx) (PHBHHx). Lauric acid was found to be the most suitable carbon source for cell growth and PHBHHx accumulation. The bacteria accumulated 49% PHBHHx containing 6% HHx in terms of cell dry weight when grown on lauric acid for 72 h. 42% PHBHHx consisting of 14% HHx was obtained with 5 g/L glucose and 10 g/L lauric acid as co-substrate. Higher glucose concentration greatly reduced the cell concentration and PHA content. The PHA biosynthesis genes from A. hydrophila WQ was successfully cloned using a two-step PCR cloning strategy based on PHA biosynthesis genes organization of Aeromonas caviae. A. hydrophila WQ and A. caviae shared high identities in the PHA gene loci, namely, ORF1, phaC and phaJ had 100%, 97% and 97.5% identities respectively. PHA synthases of A. caviae and A. hydrophila were proposed to contain type IV PHA synthases which are different compared with type I PHA synthases on the substrate specificity and location arrangement of PHA metabolic genes.


Asunto(s)
Ácido 3-Hidroxibutírico/metabolismo , Aeromonas hydrophila/genética , Aeromonas hydrophila/metabolismo , Biopolímeros/biosíntesis , Caproatos/metabolismo , Aciltransferasas/genética , Aciltransferasas/metabolismo , Aeromonas hydrophila/enzimología , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Clonación Molecular , Ácidos Láuricos/metabolismo
19.
Phytomedicine ; 21(1): 20-4, 2013 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-24035226

RESUMEN

Total paeony glucosides (TPG) extracted from the roots of Radix Paeoniae Rubrae, have been approved for the therapy of rheumatoid arthritis by the State Food and Drug Administration. We previously demonstrated the myocardial protective effects of TPG in both isoprenaline-induced myocardial ischemia rat and acute myocardial infarction rat. However, the underlying mechanism of TPG effect in cardiomyocytes remains to be investigated. The aims of this study were to elucidate the effect of TPG on the activities of antioxidant defense targets and the bioenergetic system in rat cardiomyocytes. The changes of viability, antioxidant defense system activities, protein contents, and mitochondrial functions in tert-butyl hydroperoxide challenged H9c2 rat cardiomyoblasts were evaluated. The results suggest that TPG ameliorated cardiomyoblast dysfunction by preserving antioxidant defense and bioenergetic system.


Asunto(s)
Cardiomiopatías/metabolismo , Medicamentos Herbarios Chinos/farmacología , Glucósidos/farmacología , Mitocondrias/efectos de los fármacos , Mioblastos Cardíacos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Paeonia/química , Animales , Antioxidantes/metabolismo , Antioxidantes/farmacología , Cardiomiopatías/inducido químicamente , Proteínas Musculares/metabolismo , Mioblastos Cardíacos/metabolismo , Miocardio/citología , Miocardio/metabolismo , Fitoterapia , Ratas , terc-Butilhidroperóxido
20.
Int J Biol Macromol ; 51(5): 1189-95, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-22960188

RESUMEN

The optimal conditions for sulfation of polysaccharides from persimmon fruits (PFP) with chlorosulfonic acid-pyridine (CSA-Pyr) method were determined by response surface methodology. Box-Behnken design was applied to evaluate the effects of three independent variables (volume ratio of Pyr to CSA, volume ratio of PFP to SO(3)Pyr and reaction time) on the degree of substitution (DS), molecular weight (MW) and activated partial thromboplastin time (APTT) of sulfated polysaccharides (PFP-S). The APTT activity of PFP-S could be improved by application of various volume ratio of Pyr to CSA, volume ratio of PFP to SO(3)Pyr and reaction time, which was possible due to the degradation of polysaccharides to different extent and increasing of DS. The optimal conditions to obtain the strongest APTT of PFP-S were the volume ratio of CSA to Pyr of 1:8, the volume ratio of SO(3)Pyr to PFP of 1:3.6 and the reaction time of 3 h, respectively.


Asunto(s)
Anticoagulantes/química , Anticoagulantes/farmacología , Diospyros/química , Frutas/química , Polisacáridos/química , Polisacáridos/farmacología , Sulfatos/química , Humanos , Modelos Estadísticos , Peso Molecular , Tiempo de Tromboplastina Parcial , Piridinas/química , Relación Estructura-Actividad , Ácidos Sulfónicos/química
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