RESUMEN
The current study was designed to evaluate the possible protective effects of luteolin against ß-cyfluthrin-mediated toxicity on the primary culture of rat hepatocytes (RHs). In the first step, the exposure of RHs to ß-cyfluthrin (10, 20, 40, and 80 µM) was assessed by MTT. Second, redox condition was evaluated in cotreatment of cells with luteolin (20, 40, and 60 µM) and ß-cyfluthrin (40 µM) at both medium and intra levels. In comparison to control, viability was lower in 40 and 80 µM ß-cyfluthrin-treated groups at 24 h and all ß-cyfluthrin-treated groups at 48 h (P < 0.05). Cotreatment with 20 or 40 µM luteolin + 40 µM ß-cyfluthrin resulted in a higher viability value compared to ß-cyfluthrin alone at 24 and 48 h of incubation (P < 0.05). Administration of 20 or 40 µM luteolin with ß-cyfluthrin led to the decrease of malondialdehyde and total nitrate/nitrite and the increase of total antioxidant capacity (TAC) values in both medium and intrahepatocyte levels compared to the ß-cyfluthrin-treated group at 48 h (P < 0.05). It seems that low and medium doses of luteolin possess the potential to reduce ß-cyfluthrin-mediated hepatotoxicity via attenuation of peroxidative/nitrosative reactions and augmentation of TAC levels.