Toxic Metals in Pelagic, Benthic and Demersal Fish Species from Mediterranean FAO Zone 37.

Fish represents a nutrient-rich food but, at the same time, is one of the most important contributor to the dietary intake of heavy metals. The aim of this study was to assess residual levels of Pb, Cd and Hg in different species, caught from FAO zones 37 1.3 and 37 2.2, particularly small pelagic, benthic and demersal fishes. The results obtained showed the absence of toxic metal in fishes from FAO zone 37 1.3. Relating to FAO zone 37 2.2, instead, in all samples we observed the absence of Pb, small concentrations of Cd (0.081±0.022 mg/kg) and higher Hg residual levels (0.252±0.033 mg/kg). Particularly, the trend of Cd contamination was similar in all species whereas Hg showed high levels in demersal, intermediate in pelagic and low in benthic species. However, only Cd concentrations exceed the MRL in mackerel, mullet, sea-bream fishes, according to Regulation CE n. 629/2008 and n. 488/2014.

Organochlorine pesticides and polychlorinated biphenyls in common buzzard (Buteo buteo) from Sicily (Italy).

In the present study, we investigated the concentrations and distribution of organochlorine pesticides (OCs) and polychlorinated biphenyls (PCBs) in intestine, liver, and muscle samples of 11 common buzzards (Buteo buteo) from Sicily used as bioindicator for monitoring pollution in environment. All samples of common buzzards were collected at the "Recovery Center of Wild Fauna" of Palermo, through the Zooprophilactic Institute. Quantitative determination of OCs and PCBs in the samples examined has been carried out using HRGC-ECD and GC-MS. The results obtained show the presence of concentrations of ∑DDT and ∑PCB in almost all samples. Regarding ∑DDT (4,4(')-DDE, 2,4(')-DDD, 4,4(')-DDD, 2,4(')-DDT, and 4,4(')-DDT), the highest concentrations were found in intestine (0.411 ± 0.050 µg/g) followed by muscle (0.130 ± 0.017 µg/g) and liver samples (0.109 ± 0.014 µg/g). As regards the ∑PCB congeners (PCB-28, PCB-52, PCB-95, PCB-99, PCB-101, PCB-110, PCB-138, PCB-146, PCB-149, PCB-151, PCB-153, PCB-170, PCB-177, PCB-180, PCB-183, and PCB-187), the highest concentrations were found in intestine (1.686 ± 0.144 µg/g) followed by liver (1.064 ± 0.162 µg/g) and muscle samples (0.797 ± 0.078 µg/g). Our data deserve particular attention not only for their significance but especially because they were recorded in Sicily, a region with a very low risk of environmental pollution due to the shortage of industries.

Saliva testing as noninvasive way for monitoring exercise-dependent response in teenage elite water polo players: A cohort study.

ABSTRACT: Excessively increased training volume and/or intensity and competition can lead to development of overtraining syndrome, causing a performance decrement in athletes. Tracking individual response to exercise intensity is crucial for establishing recovery strategies.We assessed the exercise intensity-dependent variability of stress response biomarkers, namely cortisol (C), testosterone (T), s-IgA, and advanced oxidation protein products (AOPP), in saliva samples of teenage elite water polo players. Saliva was collected on a day of training match (T1) and a day of competitive match (T2), at morning, before and after match.Cortisol/proteins and testosterone/proteins concentrations decreased throughout day T1, whereas increased throughout day T2. The highest values were measured after match on day T2 (2.5 ±â€Š0.5 vs 14.6 ±â€Š6.3 ng/mg; 0.061 ±â€Š0.024 vs 0.371 ±â€Š0.15 ng/mg, respectively). sIgA/proteins and AOPP/proteins concentrations increased throughout both days, and were higher after T2 match than T1 one (respectively, 1073.0 ±â€Š438.2 vs 71.0 ±â€Š17.3 µg/mg; 78.05 ±â€Š24.2 vs 15.98 ±â€Š3.16 nmol/mg, P = .003). Significant differences between concentrations of different biomarkers recorded on T1 and T2 were found only for AOPP, suggesting an increased oxidative stress on day T2. Free testosterone/cortisol ratio on day T2 was lower than that at morning (0.053 ±â€Š0.021 vs 0.107 ±â€Š0.031), indicating an increased catabolic response after competitive match.A highly significant positive correlation was found between Cortisol/Proteins and Testosterone as well as s-IgA/Proteins on day T1, and between Cortisol/Proteins and AOPP on day T2.In conclusion, we found that different types of activities, such a training or competitive session can affect the hormonal response, immunity, and oxidative stress, thereby modulating athletic performance.Our findings also confirm the usefulness of saliva testing as noninvasive way for monitoring the individual response to changes in exercise intensity in teenage elite water polo players.

Organochlorine pesticides, PCBs and heavy metals in tissues of the mullet Liza aurata in lake Ganzirri and Straits of Messina (Sicily, Italy).

The aim of this research was to investigate the presence of organochlorine pesticides, PCBs and heavy metals (Cd, Cr, Cu, Ni, Pb and Zn) in fish living in lake Ganzirri and the Straits of Messina, using the mullet Liza aurata as a "biological indicator". Different tissues of Liza aurata, which include the muscle, gill and vertebral column, were taken for analyses. Quantitative determination of the organochlorine compounds was performed by GC-ECD and confirmed with GC-MS. The concentrations of "essential" and "toxic" heavy metals were determined by Atomic Absorption Spectrophotometry. For the mullets of lake Ganzirri, the study showed DDE in three samples of gills and one of muscle, at concentrations below the MRL; no appreciable residues of PCBs (Aroclor 1232 series) were found. The concentrations of Zn and Cu were much higher than those of the "toxic" metals. No traces of organochlorine pesticides and PCBs were found in the individuals from the Straits of Messina. Therefore, these results indicate that these two environments are not at toxicological risk.

Heavy metals in liver and muscle of bluefin tunA (Thunnus thynnus) caught in the Straits of Messina (Sicily, Italy).

The present investigation was carried out on the bluefin tuna (Thunnus thynnus) collected during the month of April 2003 from the Straits of Messina. The aim of this study is to determine heavy metal levels (Cd, Cu, Hg, Mn, Pb and Zn) in liver and muscle tissues of 14 Thunnus thynnus of different age and sex and to investigate the relationships between fish sex and size (length and weight) and metal concentrations in the tissues. The concentrations of the various metals were determined by a Varian atomic absorption spectroscopy instrument. The results show significant variations (p < 0.01) for Hg and Zn concentrations in muscle than in liver samples. Concentrations of Cd and Pb are below the instrumental detection limits in almost all muscle samples. Levels of Hg (3.03 +/- 0.55 microg/g) are above the MRLs only in muscle (1 microg/g) establishing toxicological risks for the consumer. Regression analysis shows a negative correlation between mercury level and weight and length in samples from both female and male. No significant difference between mean heavy metals concentrations and male or female tuna is seen. For greater food safety, it would be advisable to reduce the mercury intake by selecting safer fish, species and size and by the avoidance of tuna fish consumption by pregnant women, young children and old people, who are more sensitive to mercury exposure.

Inhibition of lipid peroxidation by IRFI 042, a vitamin E analogue, decreases monensin cardiotoxicity in chicks.

Monensin, a well-known ionophore antibiotic, may cause severe damage in myocardial cells. We investigated whether IRFI 042, a new analogue of vitamin E, may block lipid peroxidation in myocardial cells and in turn protect against monensin toxicity. Monensin toxicity was induced by repeated daily administration of the ionophore antibiotic (150 mg/kg/day for 7 days). Sham animals received by oral gavages only a saline solution and were used as controls. All animals were randomized to receive concomitantly by oral gavages IRFI 042 (20 mg/kg) or its vehicle. The experiment lasted 8 days. Survival rate, heart lipid peroxidation, studied by means of thiobarbituric acid-reactive substances (TBARs) levels, cardiac expression of endothelial nitric oxide (e-NOS) and histological analysis of the heart were performed. Monensin administration caused a decrease in survival rate. Mortality appeared following the second monensin injection and at day 7 caused a survival rate of 20%. Thereafter, no further mortality was observed. IRFI 042 administration improved survival rate. Injection of the ionophore antibiotic resulted in a marked cardiac lipid peroxidation and in a significant reduction in cardiac e-NOS message and protein expression. IRFI 042 decreased heart TBARs levels (Monensin + vehicle = 6.5 +/- 0.8 nmol/mg; Monensin + IRFI 042 = 3.2 +/- 1.1 nmol/mg; P < 0.001) and increased e-NOS message and protein expression. Histological analysis showed that IRFI 042 improved myocardial cells damage and enhanced the depressed e-NOS expression in chick heart samples following monensin administration. Our data suggest that IRFI 042 is a promising drug to reduce monensin cardio-toxicity in chicks.