RESUMEN
Apoptosis is characterized by membrane blebbing and apoptotic body formation. Caspase cleavage of ROCK1 generates an active fragment that promotes actin-myosin-mediated contraction and membrane blebbing during apoptosis. Expression of caspase-resistant non-cleavable ROCK1 (Rock1 NC) prolonged survival of mice that rapidly develop B cell lymphomas due to Eµ-Myc transgene expression. Eµ-Myc; Rock1 NC mice had significantly fewer bone marrow cells relative to those in Eµ-Myc mice expressing wild-type ROCK1 (Rock1 WT), which was associated with altered cell cycle profiles. Circulating macrophage numbers were lower in Eµ-Myc; Rock1 NC mice, but there were higher levels of bone marrow macrophages, consistent with spontaneous cell death in Eµ-Myc; Rock1 NC mouse bone marrows being more inflammatory. Rock1 WT recipient mice transplanted with pre-neoplastic Eµ-Myc; Rock1 NC bone marrow cells survived longer than mice transplanted with Eµ-Myc; Rock1 WT cells, indicating that the survival benefit was intrinsic to the Eµ-Myc; Rock1 NC bone marrow cells. The results suggest that the apoptotic death of Eµ-Myc; Rock1 NC cells generates a proliferation-suppressive microenvironment in bone marrows that reduces cell numbers and prolongs B cell lymphoma mouse survival.
Asunto(s)
Caspasas , Linfoma de Células B , Proteínas Proto-Oncogénicas c-myc , Quinasas Asociadas a rho , Animales , Ratones , Apoptosis , Células de la Médula Ósea/metabolismo , Caspasas/metabolismo , Ciclo Celular , Linfoma de Células B/patología , Linfoma de Células B/genética , Macrófagos/metabolismo , Ratones Endogámicos C57BL , Ratones Transgénicos , Proteínas Proto-Oncogénicas c-myc/metabolismo , Quinasas Asociadas a rho/genética , Quinasas Asociadas a rho/metabolismo , Análisis de SupervivenciaRESUMEN
El uso de desinfectantes químicos y esterilizantes en la práctica dental, constituyen una parte significativa del control de la infección. Sin embargo, el número creciente de tales agentes en el mercado y las aparentes quejas conflictivas de los fabricantes, crean dificultades en la selección de los productos apropiados para el uso en el consultorio dental. Este artículo brinda una selección de desinfectantes y esterilizantes