Interactive effect of amino acids balanced at ideal lysine ratio and exogenous protease supplemented to low CP diet on growth performance, carcass traits, gut morphology, and serum metabolites in broiler chicken.

This study evaluated the effect of amino acids balanced at a specific lysine ratio with exogenous protease supplementation and reduced CP levels in the broiler diet. In total, 480 straight-run broiler chicks (Ross-308) were used for the study. A completely randomized design in the factorial arrangement was applied, with 4 treatments of 6 replicates with 20 birds each. Treatments consisted of 2 lysine ratios (100% and 110%; factor 1) without or with exogenous protease supplementation (200 g/ton; factor 2). Twenty percent reduction of CP from standard requirements of Ross-308 (18.4% vs 23% in starter and 17.2% vs 21.5% in grower phase) was made in all the treatment diets. Growth performance (feed intake, weight gain, feed conversion ratio, livability), carcass traits (live and carcass weight, carcass yield, heart, liver, gizzard, leg quarter, breast weight, and abdominal fat percentage), gut morphology (villus height, crypt depth, and villus height and crypt depth ratio), and serum metabolites (total protein, albumin, and uric acid) were evaluated. A significant interaction (p ≤ 0.05) for lysine ratio and protease was noted in body weight gain, feed conversion ratio, carcass traits, and gut morphology throughout the experimental period yet a non-significant (p > 0.05) influence was observed on serum metabolites. It was concluded that the broiler diet with a net reduction of 20% CP from Ross-308 standards, balanced for amino acids at lysine ratio 110% and supplemented with exogenous protease, could be used to improve growth performance, intestinal health, and carcass traits.

Mapping CircRNA-miRNA-mRNA regulatory axis identifies hsa_circ_0080942 and hsa_circ_0080135 as a potential theranostic agents for SARS-CoV-2 infection.

Non-coding RNAs (ncRNAs) can control the flux of genetic information; affect RNA stability and play crucial roles in mediating epigenetic modifications. A number of studies have highlighted the potential roles of both virus-encoded and host-encoded ncRNAs in viral infections, transmission and therapeutics. However, the role of an emerging type of non-coding transcript, circular RNA (circRNA) in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has not been fully elucidated so far. Moreover, the potential pathogenic role of circRNA-miRNA-mRNA regulatory axis has not been fully explored as yet. The current study aimed to holistically map the regulatory networks driven by SARS-CoV-2 related circRNAs, miRNAs and mRNAs to uncover plausible interactions and interplay amongst them in order to explore possible therapeutic options in SARS-CoV-2 infection. Patient datasets were analyzed systematically in a unified approach to explore circRNA, miRNA, and mRNA expression profiles. CircRNA-miRNA-mRNA network was constructed based on cytokine storm related circRNAs forming a total of 165 circRNA-miRNA-mRNA pairs. This study implies the potential regulatory role of the obtained circRNA-miRNA-mRNA network and proposes that two differentially expressed circRNAs hsa_circ_0080942 and hsa_circ_0080135 might serve as a potential theranostic agents for SARS-CoV-2 infection. Collectively, the results shed light on the functional role of circRNAs as ceRNAs to sponge miRNA and regulate mRNA expression during SARS-CoV-2 infection.