RESUMEN
BACKGROUND: Spondyloarthritis (SpA) is a common debilitating inflammatory disorder. Establishing the diagnosis is often difficult, since abnormalities in conventional X-ray develop with a latency of several years and only HLA-B27 is used as a laboratory marker. The goal of our study was to identify new autoantibodies as diagnostic markers of SpA. METHODS: Protein array technology was used to screen for new autoantigens in ankylosing spondylitis. Then, the results were confirmed by ELISA using Class II-associated invariant chain peptide domain of CD74 as antigen. Sera for the ELISA were obtained from 216 patients with axial (n=156) and peripheral (n=60) SpA. Sera of patients with psoriatic arthritis without axial involvement as another subtype of peripheral SpA, rheumatoid arthritis, systemic lupus erythematosus, HIV infection and blood donors served as controls. All donors provided informed consent for the study which was approved by the local ethics committee (project number 4928). RESULTS: Using protein arrays, we detected IgG antibodies against CD74 in SpA sera. Using ELISA technology on sera that had previously been frozen for several years, IgG autoantibodies against CD74 were found in 67% of the SpA patients and were even more frequent in patients with a short disease duration. In the controls, the prevalence of the new autoantibodies was 18/40 (45%) in psoriatic arthritis without axial involvement, 9/80 (11%) in rheumatoid arthritis, 6/40 (15%) in systemic lupus erythematosus, 1/40 (2.5%) in HIV and 1/125 (0.8%) in blood donors. CONCLUSIONS: Antibodies against CD74 could provide an important additional tool for diagnosis of SpA.
Asunto(s)
Antígenos de Diferenciación de Linfocitos B/inmunología , Autoanticuerpos/inmunología , Antígenos de Histocompatibilidad Clase II/inmunología , Inmunoglobulina G/inmunología , Espondiloartritis/inmunología , Adulto , Anciano , Artritis Psoriásica/diagnóstico , Artritis Psoriásica/inmunología , Artritis Psoriásica/fisiopatología , Biomarcadores , Estudios de Casos y Controles , Ensayo de Inmunoadsorción Enzimática , Femenino , Antígeno HLA-B27/inmunología , Humanos , Lupus Eritematoso Sistémico/inmunología , Masculino , Persona de Mediana Edad , Espondiloartritis/diagnóstico , Espondiloartritis/fisiopatología , Espondiloartropatías/diagnóstico , Espondiloartropatías/inmunología , Espondiloartropatías/fisiopatología , Adulto JovenAsunto(s)
Neoplasias de los Bronquios/secundario , Melanoma/secundario , Neoplasias Primarias Desconocidas/diagnóstico , Bronquios/patología , Neoplasias de los Bronquios/diagnóstico , Neoplasias de los Bronquios/patología , Broncoscopía , Diagnóstico Diferencial , Infecciones por VIH/diagnóstico , Infecciones por VIH/patología , Humanos , Linfoma Cutáneo de Células T/diagnóstico , Linfoma Cutáneo de Células T/patología , Masculino , Melanoma/diagnóstico , Melanoma/patología , Persona de Mediana Edad , Neoplasias Primarias Desconocidas/patología , Cuidados Paliativos , Mucosa Respiratoria/patologíaRESUMEN
Background | Currently only estimates exist of seroprevalence of syphilis and human immunodeficiency virus (HIV) in refugees arriving in Germany during the current refugee crisis. Objectives | To assess the prevalence of syphilis and human immunodeficiency virus (HIV) in refugees arriving in northern Germany in 2015. Materials and methods | In a cross-sectional study in 790 patients from all age groups tests for serological markers of treponema pallidum and in 789 patients for human immunodeficiency virus (HIV) were performed in August 2015 in reception centers in northern Germany. Results | The overall prevalence of treponema pallidum antibodies was 0.13 % (1/790; [95 % CI: 0 - 0.4]). HIV antibodies were positive in two refugees from sub-Saharan Africa (2/789; 0.25 %, [95 % CI: 0 - 0.6]). Conclusions | This study showed a low prevalence of treponema pallidum antibodies and human immunodeficiency virus infection (HIV) in a German refugee cohort, not significantly different from German controls.