RESUMEN
The gene responsible for Friedreich's ataxia, a disease characterized by neurodegeneration and cardiomyopathy, has recently been cloned and its product designated frataxin. A gene in Saccharomyces cerevisiae was characterized whose predicted protein product has high sequence similarity to the human frataxin protein. The yeast gene (yeast frataxin homolog, YFH1) encodes a mitochondrial protein involved in iron homeostasis and respiratory function. Human frataxin also was shown to be a mitochondrial protein. Characterizing the mechanism by which YFH1 regulates iron homeostasis in yeast may help to define the pathologic process leading to cell damage in Friedreich's ataxia.
Asunto(s)
Ceruloplasmina , Proteínas Fúngicas/metabolismo , Proteínas de Unión a Hierro , Hierro/metabolismo , Mitocondrias/metabolismo , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/metabolismo , Transporte Biológico , Proteínas Portadoras/metabolismo , Membrana Celular/metabolismo , Citosol/metabolismo , Ataxia de Friedreich/metabolismo , Proteínas Fúngicas/genética , Genes Fúngicos , Prueba de Complementación Genética , Homeostasis , Humanos , Proteínas de Transporte de Membrana/metabolismo , Estrés Oxidativo , Oxidorreductasas/metabolismo , Fosfotransferasas (Aceptor de Grupo Alcohol)/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/crecimiento & desarrollo , Transformación Genética , FrataxinaRESUMEN
Legal and theological definitions of sodomy in the Massachusetts Bay Colony, and various recommendations for its punishment, are discussed. A notorious case of heterosexual child molestation illustrates the differing views as to definitions of the crime and the requirements for imposing the death penalty. The author suggests that the strict legal procedures later established in order to avoid the confusion attendant upon this case actually reduced the number of arrests and convictions for homosexual activity.