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1.
EMBO J ; 41(17): e109205, 2022 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-35880301

RESUMEN

Patient-derived organoids and cellular spheroids recapitulate tissue physiology with remarkable fidelity. We investigated how engagement with a reconstituted basement membrane in three dimensions (3D) supports the polarized, stress resilient tissue phenotype of mammary epithelial spheroids. Cells interacting with reconstituted basement membrane in 3D had reduced levels of total and actin-associated filamin and decreased cortical actin tension that increased plasma membrane protrusions to promote negative plasma membrane curvature and plasma membrane protein associations linked to protein secretion. By contrast, cells engaging a reconstituted basement membrane in 2D had high cortical actin tension that forced filamin unfolding and endoplasmic reticulum (ER) associations. Enhanced filamin-ER interactions increased levels of PKR-like ER kinase effectors and ER-plasma membrane contact sites that compromised calcium homeostasis and diminished cell viability. Consequently, cells with decreased cortical actin tension had reduced ER stress and survived better. Consistently, cortical actin tension in cellular spheroids regulated polarized basement membrane membrane deposition and sensitivity to exogenous stress. The findings implicate cortical actin tension-mediated filamin unfolding in ER function and underscore the importance of tissue mechanics in organoid homeostasis.


Asunto(s)
Actinas , Retículo Endoplásmico , Actinas/metabolismo , Retículo Endoplásmico/metabolismo , Estrés del Retículo Endoplásmico , Células Epiteliales/metabolismo , Filaminas/metabolismo , Fenotipo
2.
Nat Rev Mol Cell Biol ; 15(12): 771-85, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25370693

RESUMEN

The biochemical and biophysical properties of the extracellular matrix (ECM) dictate tissue-specific cell behaviour. The molecules that are associated with the ECM of each tissue, including collagens, proteoglycans, laminins and fibronectin, and the manner in which they are assembled determine the structure and the organization of the resultant ECM. The product is a specific ECM signature that is comprised of unique compositional and topographical features that both reflect and facilitate the functional requirements of the tissue.


Asunto(s)
Matriz Extracelular/química , Matriz Extracelular/metabolismo , Animales , Membrana Basal/química , Membrana Basal/metabolismo , Proteínas de la Matriz Extracelular/química , Proteínas de la Matriz Extracelular/metabolismo , Humanos , Neuronas/citología , Proteoglicanos/metabolismo
3.
Bioessays ; 37(12): 1293-7, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26439949

RESUMEN

Recent work by Fernández-Sánchez and coworkers examining the impact of applied pressure on the malignant phenotype of murine colon tissue in vivo revealed that mechanical perturbations can drive malignant behavior in genetically normal cells. Their findings build upon an existing understanding of how the mechanical cues experienced by cells within a tissue become progressively modified as the tissue transforms. Using magnetically stimulated ultra-magnetic liposomes to mimic tumor growth -induced solid stress, Fernández-Sánchez and coworkers were able to stimulate ß-catenin to promote the cancerous behavior of both a normal and genetically modified colon epithelium. In this perspective, we discuss their findings in the context of what is currently known regarding the role of the mechanical landscape in cancer progression and ß-catenin as a mechanotransducer. We review data that suggest that mechanically regulated activation of ß-catenin fosters development of a malignant phenotype in tissue and predict that mechanical cues may contribute to tumor heterogeneity.


Asunto(s)
Neoplasias/genética , Neoplasias/patología , Transducción de Señal/genética , beta Catenina/genética , Animales , Progresión de la Enfermedad , Humanos , Fenotipo
4.
Biochemistry ; 53(13): 2078-90, 2014 Apr 08.
Artículo en Inglés | MEDLINE | ID: mdl-24649923

RESUMEN

Cells in vivo exist within the context of a multicellular tissue, where their behavior is governed by homo- and heterotypic cell-cell interactions, the material properties of the extracellular matrix, and the distribution of various soluble and physical factors. Most methods currently used to study and manipulate cellular behavior in vitro, however, sacrifice physiological relevance for experimental expediency. The fallacy of such approaches has been highlighted by the recent development and application of three-dimensional culture models to cell biology, which has revealed striking phenotypic differences in cell survival, migration, and differentiation in genetically identical cells simply by varying culture conditions. These perplexing findings beg the question of what constitutes a three-dimensional culture and why cells behave so differently in two- and three-dimensional culture formats. In the following review, we dissect the fundamental differences between two- and three-dimensional culture conditions. We begin by establishing a basic definition of what "three dimensions" means at different biological scales and discuss how dimensionality influences cell signaling across different length scales. We identify which three-dimensional features most potently influence intracellular signaling and distinguish between conserved biological principles that are maintained across culture conditions and cellular behaviors that are sensitive to microenvironmental context. Finally, we highlight state-of-the-art molecular tools amenable to the study of signaling in three dimensions under conditions that facilitate deconstruction of signaling in a more physiologically relevant manner.


Asunto(s)
Técnicas de Cultivo de Célula , Imagenología Tridimensional/métodos , Transducción de Señal , Adhesión Celular , Forma de la Célula , Difusión , Células Epiteliales/citología , Matriz Extracelular/química , Matriz Extracelular/metabolismo , Humanos , Proteínas/química , Proteínas/metabolismo
5.
Mol Biol Cell ; 28(11): 1467-1488, 2017 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-28381423

RESUMEN

Metastasis requires tumor cells to navigate through a stiff stroma and squeeze through confined microenvironments. Whether tumors exploit unique biophysical properties to metastasize remains unclear. Data show that invading mammary tumor cells, when cultured in a stiffened three-dimensional extracellular matrix that recapitulates the primary tumor stroma, adopt a basal-like phenotype. Metastatic tumor cells and basal-like tumor cells exert higher integrin-mediated traction forces at the bulk and molecular levels, consistent with a motor-clutch model in which motors and clutches are both increased. Basal-like nonmalignant mammary epithelial cells also display an altered integrin adhesion molecular organization at the nanoscale and recruit a suite of paxillin-associated proteins implicated in invasion and metastasis. Phosphorylation of paxillin by Src family kinases, which regulates adhesion turnover, is similarly enhanced in the metastatic and basal-like tumor cells, fostered by a stiff matrix, and critical for tumor cell invasion in our assays. Bioinformatics reveals an unappreciated relationship between Src kinases, paxillin, and survival of breast cancer patients. Thus adoption of the basal-like adhesion phenotype may favor the recruitment of molecules that facilitate tumor metastasis to integrin-based adhesions. Analysis of the physical properties of tumor cells and integrin adhesion composition in biopsies may be predictive of patient outcome.


Asunto(s)
Adhesión Celular/fisiología , Integrinas/metabolismo , Paxillin/metabolismo , Mama/metabolismo , Neoplasias de la Mama/patología , Línea Celular Tumoral , Matriz Extracelular/metabolismo , Femenino , Proteína-Tirosina Quinasas de Adhesión Focal/metabolismo , Humanos , Metástasis de la Neoplasia/fisiopatología , Fosforilación , Transducción de Señal
6.
Integr Biol (Camb) ; 8(7): 795-804, 2016 07 11.
Artículo en Inglés | MEDLINE | ID: mdl-27334548

RESUMEN

The mechanical properties of the extracellular matrix influence cell signaling to regulate key cellular processes, including differentiation, apoptosis, and transformation. Understanding the molecular mechanisms underlying mechanotransduction is contingent upon our ability to visualize the effect of altered matrix properties on the nanoscale organization of proteins involved in this signalling. The development of super-resolution imaging techniques has afforded researchers unprecedented ability to probe the organization and localization of proteins within the cell. However, most of these methods require use of substrates like glass or silicon wafers, which are artificially rigid. In light of a growing body of literature demonstrating the importance of mechanical properties of the extracellular matrix in regulating many aspects of cellular behavior and signaling, we have developed a system that allows scanning angle interference microscopy on a mechanically tunable substrate. We describe its implementation in detail and provide examples of how it may be used to aide investigations into the effect of substrate rigidity on intracellular signaling.


Asunto(s)
Adhesión Celular/efectos de la radiación , Matriz Extracelular/fisiología , Matriz Extracelular/ultraestructura , Micromanipulación/métodos , Nanopartículas/ultraestructura , Geles de Silicona/química , Línea Celular , Células Epiteliales/citología , Células Epiteliales/fisiología , Humanos , Aumento de la Imagen , Mecanotransducción Celular/fisiología , Microscopía de Fuerza Atómica , Microscopía de Interferencia , Resistencia al Corte , Estrés Mecánico , Resistencia a la Tracción/fisiología
7.
J Biotechnol ; 193: 66-9, 2015 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-25435379

RESUMEN

Extracellular matrix (ECM) structure, composition, and stiffness have profound effects on tissue development and pathologies such as cardiovascular disease and cancer. Accordingly, a variety of synthetic hydrogel systems have been designed to study the impact of ECM composition, density, mechanics, and topography on cell and tissue phenotype. However, these synthetic systems fail to accurately recapitulate the biological properties and structure of the native tissue ECM. Natural three dimensional (3D) ECM hydrogels, such as collagen or hyaluronic acid, feature many of the chemical and physical properties of tissue, yet, these systems have limitations including the inability to independently control biophysical properties such as stiffness and pore size. Here, we present a 3D tension bioreactor system that permits precise mechanical tuning of collagen hydrogel stiffness, while maintaining consistent composition and pore size. We achieve this by mechanically loading collagen hydrogels covalently-conjugated to a polydimethylsiloxane (PDMS) membrane to induce hydrogel stiffening. We validated the biological application of this system with oncogenically transformed mammary epithelial cell organoids embedded in a 3D collagen I hydrogel, either uniformly stiffened or calibrated to create a gradient of ECM stiffening, to visually demonstrate the impact of ECM stiffening on transformation and tumor cell invasion. As such, this bioreactor presents the first tunable 3D natural hydrogel system that is capable of independently assessing the role of ECM stiffness on tissue phenotype.


Asunto(s)
Reactores Biológicos , Técnicas de Cultivo de Célula/instrumentación , Colágeno/química , Hidrogel de Polietilenoglicol-Dimetacrilato/química , Neoplasias/química , Técnicas de Cultivo de Tejidos/instrumentación , Animales , Línea Celular Tumoral , Elasticidad , Matriz Extracelular/química , Mamíferos , Organoides/citología , Porosidad
8.
Cancer Res ; 74(17): 4597-611, 2014 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-25183785

RESUMEN

Extracellular matrix (ECM) stiffness induces focal adhesion assembly to drive malignant transformation and tumor metastasis. Nevertheless, how force alters focal adhesions to promote tumor progression remains unclear. Here, we explored the role of the focal adhesion protein vinculin, a force-activated mechanotransducer, in mammary epithelial tissue transformation and invasion. We found that ECM stiffness stabilizes the assembly of a vinculin-talin-actin scaffolding complex that facilitates PI3K-mediated phosphatidylinositol (3,4,5)-triphosphate phosphorylation. Using defined two- and three-dimensional matrices, a mouse model of mammary tumorigenesis with vinculin mutants, and a novel super resolution imaging approach, we established that ECM stiffness, per se, promotes the malignant progression of a mammary epithelium by activating and stabilizing vinculin and enhancing Akt signaling at focal adhesions. Our studies also revealed that vinculin strongly colocalizes with activated Akt at the invasive border of human breast tumors, where the ECM is stiffest, and we detected elevated mechanosignaling. Thus, ECM stiffness could induce tumor progression by promoting the assembly of signaling scaffolds, a conclusion underscored by the significant association we observed between highly expressed focal adhesion plaque proteins and malignant transformation across multiple types of solid cancer. See all articles in this Cancer Research section, "Physics in Cancer Research."


Asunto(s)
Fosfatidilinositol 3-Quinasas/metabolismo , Fosfatos de Fosfatidilinositol/metabolismo , Vinculina/metabolismo , Actinas/metabolismo , Animales , Neoplasias de la Mama/metabolismo , Neoplasias de la Mama/patología , Carcinogénesis/metabolismo , Carcinogénesis/patología , Adhesión Celular/fisiología , Línea Celular , Transformación Celular Neoplásica/metabolismo , Transformación Celular Neoplásica/patología , Progresión de la Enfermedad , Epitelio/metabolismo , Epitelio/patología , Matriz Extracelular/metabolismo , Matriz Extracelular/patología , Femenino , Adhesiones Focales/metabolismo , Humanos , Glándulas Mamarias Animales/metabolismo , Glándulas Mamarias Animales/patología , Glándulas Mamarias Humanas/metabolismo , Glándulas Mamarias Humanas/patología , Ratones , Fosforilación/fisiología , Talina/metabolismo
9.
Nat Med ; 20(4): 360-7, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-24633304

RESUMEN

Tissue mechanics regulate development and homeostasis and are consistently modified in tumor progression. Nevertheless, the fundamental molecular mechanisms through which altered mechanics regulate tissue behavior and the clinical relevance of these changes remain unclear. We demonstrate that increased matrix stiffness modulates microRNA expression to drive tumor progression through integrin activation of ß-catenin and MYC. Specifically, in human and mouse tissue, increased matrix stiffness induced miR-18a to reduce levels of the tumor suppressor phosphatase and tensin homolog (PTEN), both directly and indirectly by decreasing levels of homeobox A9 (HOXA9). Clinically, extracellular matrix stiffness correlated directly and significantly with miR-18a expression in human breast tumor biopsies. miR-18a expression was highest in basal-like breast cancers in which PTEN and HOXA9 levels were lowest, and high miR-18a expression predicted poor prognosis in patients with luminal breast cancers. Our findings identify a mechanically regulated microRNA circuit that can promote malignancy and suggest potential prognostic roles for HOXA9 and miR-18a levels in stratifying patients with luminal breast cancers.


Asunto(s)
Elasticidad , Matriz Extracelular/metabolismo , Regulación Neoplásica de la Expresión Génica , MicroARNs/genética , Fosfohidrolasa PTEN/metabolismo , Microambiente Tumoral , Animales , Neoplasias de la Mama , Línea Celular , Progresión de la Enfermedad , Matriz Extracelular/genética , Femenino , Proteínas de Homeodominio/metabolismo , Humanos , Glándulas Mamarias Animales/metabolismo , Glándulas Mamarias Humanas/metabolismo , Ratones , MicroARNs/fisiología , Metástasis de la Neoplasia/genética , Proteína Oncogénica p55(v-myc)/metabolismo , beta Catenina/metabolismo
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