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1.
Andrologia ; 48(10): 1202-1213, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26992476

RESUMEN

This study investigated the effect of quercetin on changes in testes due to arsenic exposure. Twenty-seven male rats were divided into three groups: control (10 ml kg-1  day-1 saline), arsenic (10 mg kg-1  day-1 sodium arsenite) and arsenic + quercetin (arsenic + 50 mg kg-1  day-1 quercetin). The rats were sacrificed at the end of 15-day experiment. There was no difference between control group and arsenic group in body weight gain, testicular weight and serum total testosterone level. Quercetin treatment did not cause a significant difference in these parameters. In the arsenic group rats, we determined deterioration in the structure of seminiferous tubules, a decrease in the number of spermatogenic cells, an increase in the number of apoptotic cells, a decrease in the number of PCNA-positive cells, a decrease in SOD, CAT and GSH-Px activities, and an increase in the MDA level in testicular tissue. In all these changes, arsenic+quercetin group showed an improved compared to arsenic group. The amount of arsenic increased in the arsenic group was compared to the control group, and there was no difference between arsenic group and arsenic + quercetin group in the amount of arsenic. In conclusion, quercetin prevented arsenic-induced testicular damage with its anti-apoptotic and antioxidant effects.


Asunto(s)
Antioxidantes/farmacología , Arsenitos/farmacología , Estrés Oxidativo/efectos de los fármacos , Sustancias Protectoras/farmacología , Quercetina/farmacología , Compuestos de Sodio/farmacología , Espermatogénesis/efectos de los fármacos , Testículo/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Peso Corporal/efectos de los fármacos , Catalasa/metabolismo , Glutatión Peroxidasa/metabolismo , Masculino , Malondialdehído/metabolismo , Ratas , Ratas Sprague-Dawley , Superóxido Dismutasa/metabolismo , Testículo/metabolismo , Testosterona/sangre
2.
Andrologia ; 48(2): 222-30, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26011095

RESUMEN

The objective of this study was to examine the effects of thymoquinone (TQ), which has antioxidant properties in the experimental testicular I/R model in rats in terms of its anti-apoptotic, proliferative and biochemical attributes. In our study, 24 male rats were divided into three groups: control group, I/R group and I/R+TQ group. Testicular torsion was created by rotating the left testis 720° in a clockwise direction. The ischaemia period was 4 h, and an orchiectomy was performed after 4 h of detorsion. Spermatogenesis and the mean seminiferous tubule diameter were significantly decreased in the I/R groups compared to the control group. Furthermore, TQ-treated animals displayed an improved histological appearance in the I/R group. It was also observed that treatment with TQ increased the activity of PCNA, which decreased as a result of I/R, and this treatment also reduced the number of TUNEL-positive cells. The I/R+TQ group showed a decrease in malondialdehyde levels and an increase in the activities of superoxide dismutase, catalase and glutathione peroxidase in comparison with the I/R group. It could be concluded that cytoprotective effects of TQ on the I/R testicles are via reduction of apoptosis, oxidative stress and lipid peroxidation.


Asunto(s)
Antioxidantes/farmacología , Apoptosis/efectos de los fármacos , Benzoquinonas/farmacología , Proliferación Celular/efectos de los fármacos , Daño por Reperfusión/metabolismo , Espermatogénesis/efectos de los fármacos , Espermatozoides/efectos de los fármacos , Testículo/efectos de los fármacos , Animales , Catalasa/efectos de los fármacos , Catalasa/metabolismo , Glutatión Peroxidasa/efectos de los fármacos , Glutatión Peroxidasa/metabolismo , Etiquetado Corte-Fin in Situ , Masculino , Malondialdehído/metabolismo , Orquiectomía , Tamaño de los Órganos , Antígeno Nuclear de Célula en Proliferación/efectos de los fármacos , Antígeno Nuclear de Célula en Proliferación/metabolismo , Ratas , Ratas Sprague-Dawley , Daño por Reperfusión/patología , Túbulos Seminíferos/efectos de los fármacos , Túbulos Seminíferos/patología , Espermatozoides/metabolismo , Espermatozoides/patología , Superóxido Dismutasa/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Testículo/metabolismo , Testículo/patología
3.
Andrologia ; 46(4): 356-69, 2014 May.
Artículo en Inglés | MEDLINE | ID: mdl-23488863

RESUMEN

The aim of this study was to investigate the effects of quercetin and fish n-3 fatty acids on the changes in testis induced by ethanol. Forty-five rats divided into five groups, control, ethanol, ethanol+quercetin, ethanol+fish n-3 fatty acids and ethanol+quercetin+fish n-3 fatty acids. At the end of 8 weeks, all the rats were sacrificed. Degenerative changes in histopathological analyses, the decreased body weight gain and seminiferous tubule diameters in ethanol group have been observed. TUNEL assay also showed an increase in apoptotic cell number. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), xanthine oxidase (XO) and testosterone levels were decreased as well as the levels of malondialdehyde (MDA) and nitric oxide (NO) were increased in ethanol group. Histopathological changes caused by ethanol have been improved by quercetin and fish n-3 fatty acids. It was also found that protection was provided by increasing SOD, CAT and GSH-Px activities in groups administered quercetin, fish n-3 fatty acids and quercetin+fish n-3 fatty acids, and by decreasing the levels of MDA and NO in groups administered both quercetin and fish n-3 fatty acids together. These results suggest that quercetin and fish n-3 fatty acids are beneficial agents to reduce testicular injury induced by ethanol except for testosterone levels.


Asunto(s)
Etanol/efectos adversos , Ácidos Grasos Omega-3/farmacología , Quercetina/farmacología , Testículo/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Catalasa/metabolismo , Glutatión Peroxidasa/metabolismo , Masculino , Malondialdehído/metabolismo , Tamaño de los Órganos , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismo , Testículo/patología , Testosterona/sangre , Xantina Oxidasa/metabolismo
4.
Andrologia ; 46(8): 917-26, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24117968

RESUMEN

The aim of this study was to examine the protective effects of fish omega-3 (n-3) fatty acids on acute doxorubicin (DOX)-induced testicular apoptosis and oxidative damage. 24 male rats were divided into three groups: control, DOX-treated and DOX+fish n-3 fatty acids. Fish n-3 fatty acids (400 mg kg(-1) ) were given for 30 days by intragastric gavage. The rats received a single intraperitoneal injection of DOX (30 mg kg(-1) ) and were sacrificed after 48 h. The DOX+fish n-3 fatty acids group showed a decrease in malondialdehyde levels and increased activities of superoxide dismutase and glutathione peroxidase in comparison with the DOX-treated group. Acute DOX treatment caused severe damage such as disorganisation and separation of germ cells. The fish n-3 fatty acids-pretreated rats showed an improved histological appearance in the DOX-treated group. Our data indicate a reduction in the activity of terminal deoxynucleotidyl transferase mediated dUTP nick end labelling; there was a rise in the expression of proliferating cell nuclear antigen in testis tissues of the DOX+fish n-3 fatty acids group compared with DOX-treated group. These data suggested that fish n-3 fatty acids pre-treatment may be beneficial for spermatogenesis following acute DOX-induced testicular damage by decreasing germ cell apoptosis and oxidative stress.


Asunto(s)
Apoptosis/efectos de los fármacos , Ácidos Grasos Omega-3/uso terapéutico , Estrés Oxidativo/efectos de los fármacos , Enfermedades Testiculares/prevención & control , Animales , Doxorrubicina , Ácidos Grasos Omega-3/farmacología , Masculino , Distribución Aleatoria , Ratas Sprague-Dawley , Enfermedades Testiculares/inducido químicamente
5.
Folia Morphol (Warsz) ; 73(3): 331-8, 2014 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-25465038

RESUMEN

BACKGROUND: The aim of this study is to assess and document the dimensions of the normal spleen measured on computed tomography (CT) images with the normal splenic volume measured by Cavalieri principle on CT images and thereby serve as a baseline for comparison in cases of splenomegaly using abdominal CT. To investigate the relationship between these changes and body mass index, gender, abdominal diameters. MATERIALS AND METHODS: We retrospectively reviewed abdominal CT examinations of 212 adults between the ages of 20 and 88 years. There were seven groups of patients. The spleen volume (SV) measurements using abdominal CT images of each patient on the Image Information Systems were performed with Cavalieri principle. RESULTS: The mean SV and splenic length (SL), width (SW), and thickness (ST) for the total study population of 212 patients was 198 ± 88 cm³, 9.96 ± 2.1 cm, 8.87 ±± 1.6 cm and 4.58 ± 0.8 cm, respectively. There was a strong correlation between SV and ST (r = 0.752, p < 0.001), SL (r = 0.735, p < 0.001), SW (r = 0.681,p < 0.001) mean values of total study population. Comparison between mean splenic dimension parameters for males and females showed a statistically significant difference (p = 0.032 for SV, p = 0.04 for ST) but no statistically significant difference with SL and SW. Also there was a positive correlation between SV and body height, sagittal abdominal diameter and transvers abdominal diameter in mean of total groups and female groups, there was no correlation in males. CONCLUSIONS: The normal reference ranges for SV and size given in this study canserve as a standard to judge whether splenomegaly is present in patients.

7.
Physiol Res ; 53(4): 403-8, 2004.
Artículo en Inglés | MEDLINE | ID: mdl-15311999

RESUMEN

Melatonin, the main hormone of the pineal gland, informs the body about the environmental light and darkness regimen, which in turn contributes to the photoperiodic adaptation of several physiological functions. Leptin, the hormone secreted mainly by adipocytes and some other tissues including the pituitary, informs the brain about the mass of adipose tissue, which plays an important role in energy homeostasis. Melatonin has been shown to decrease circulating leptin levels. It is currently not known whether melatonin has an effect on leptin synthesis in the pituitary. The aim of this study was to immunohistochemically examine the effects of pinealectomy and administration of melatonin on leptin production in the rat anterior pituitary. The pituitary samples obtained from 18 male Wistar rats including sham-pinealectomized, pinealectomized and melatonin-injected pinealectomized groups were immunohistochemically evaluated. Immunostaining of leptin was moderate (3+) in sham-pinealectomized rats, heavy (5+) in pinealectomized rats and low (1+) in melatonin-treated pinealectomized rats, respectively. The present results indicate that pinealectomy induces leptin secretion in anterior pituitary cells, and this increase of leptin synthesis can be prevented by administration of melatonin. Thus, melatonin seems to have both physiological and pharmacological effects on leptin production in the anterior pituitary of male rats.


Asunto(s)
Leptina/biosíntesis , Melatonina/farmacología , Glándula Pineal/metabolismo , Adenohipófisis/metabolismo , Animales , Inmunohistoquímica , Leptina/análisis , Leptina/antagonistas & inhibidores , Masculino , Glándula Pineal/química , Glándula Pineal/cirugía , Adenohipófisis/química , Adenohipófisis/efectos de los fármacos , Ratas , Ratas Wistar
8.
Hum Exp Toxicol ; 33(4): 435-45, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-24064909

RESUMEN

The aim of this study was to investigate the protective effects of fish omega-3 (n-3) fatty acids on doxorubicin (DOX)-induced acute cardiotoxicity. A total of 24 rats were divided into three groups: control, DOX-treated, and DOX treated with fish n-3 fatty acids. Control group received 0.4 ml/kg/day of saline intragastrically. The rats in the fish n-3 fatty acid-pretreated group were given 400 mg/kg/day fish n-3 fatty acids for 30 days by intragastric intubation. To induce acute cardiotoxicity, DOX (30 mg/kg) was injected intraperitoneally by a single dose and the rats were killed after 48 h. DOX treatment caused severe damage in heart tissues. Disorganization of myocardial muscle fibers, myofibrillar loss, and cardiotoxic myocardial fibers with cytoplasmic vacuoles were seen. Fish n-3 fatty acid-treated rats showed an improved histological appearance in the DOX-treated group. Our data indicate a significant reduction in the activity of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling in cardiomyocytes of the DOX-treated group with fish n-3 fatty acids therapy. The DOX-treated with fish n-3 fatty acids group showed a significant decrease in malondialdehyde levels, and an increase in superoxide dismutase and glutathione peroxidase activities in comparison with the DOX-treated group. This study showed that fish n-3 fatty acids may be a suitable cardioprotector against acute toxic effects of DOX.


Asunto(s)
Antibióticos Antineoplásicos/toxicidad , Cardiotónicos/farmacología , Doxorrubicina/toxicidad , Ácidos Grasos Omega-3/farmacología , Corazón/efectos de los fármacos , Animales , Masculino , Ratas , Ratas Sprague-Dawley
9.
Arch Androl ; 53(2): 79-82, 2007.
Artículo en Inglés | MEDLINE | ID: mdl-17453686

RESUMEN

The aim of this study was to immunohistochemically examine the effects of orchidectomy and administration of testosterone hormone on leptin production in the rat anterior pituitary. Twenty-one male Wistar rats were divided into three groups. Group I and group II were designated as control (sham-orchidectomized) and orchidectomized rats, respectively. Rats in group III were orchidectomized and injected daily with testosterone propionate for 1 month. At the end of the experimental period, all animals were sacrificed by decapitation. The pituitary glands of all rats were removed and processed for semi-quantitative evaluation of immunohistochemical leptin staining. Intensity of immunostaining was determined on a scale between 0 (no staining) and 5 (heavy staining). Immunostaining of leptin was moderate (3+) in control rats, heavy (5+) in orchidectomized rats, and low (1+) in testosterone-treated orchidectomized rats, respectively. These findings indicate that orchidectomy increases leptin secretion in anterior pituitary cells, and this increase of leptin synthesis can be prevented by administration of testosterone propionate. Thus, testosterone seems to affect leptin production in the anterior pituitary of male rats.


Asunto(s)
Leptina/biosíntesis , Adenohipófisis/metabolismo , Testosterona/farmacología , Animales , Inmunohistoquímica , Masculino , Orquiectomía , Adenohipófisis/citología , Adenohipófisis/efectos de los fármacos , Ratas , Ratas Wistar , Propionato de Testosterona/farmacología
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