Effects of glucose and fructose on recently reactivated and recently acquired memories.

1. The effects of glucose and fructose on memory reactivation were investigated. 2. Rats were trained originally on a brightness discrimination passive avoidance task. 3. Memory reactivation treatment consisted of re-exposing the rats 24 hr later to the footshock unconditioned stimulus in the experimental room. Glucose or fructose (32, 100, 320, 1000, or 2000 mg/kg) was administered immediately after reactivation. 4. Twenty-four hr after reactivation (48 hr after training) the rats were tested for their ability to acquire an active avoidance (reversal) task. 5. The dose-response functions for the effects of both glucose and fructose on the reactivated memory followed identical cubic trends. However, a combined dose of glucose and fructose was significantly less effective at modulating memory than was an equimolar dose of either sugar alone. 6. We compared analytically the effects of combined glucose and fructose treatment on new versus old memories. The dose-response functions for both types of memories follow cubic trends, suggesting that similar multiple interacting mechanisms operate when memories are originally stored and when they are later re-encoded.

Time-dependent effects of fructose on the modulation of a reactivated memory.

1. A passive-avoidance-to-active-avoidance negative transfer paradigm was used to investigate systematically the time-dependent effects of fructose on reactivated memories in rats. 2. Memory reactivation consisted of re-exposing the rats 24 hr after passive-avoidance conditioning to environmental and learning cues present during training; post-reactivation injections of fructose (100 mg/kg, sc) or saline were followed 24 hr later by active-avoidance (discrimination reversal) conditioning. Fructose or saline was administered in the experimental room 0, 2, 5, or 30 min, or in the colony room 60 min, after reactivation. 3. The results showed a time-dependent decrease in the ability of fructose to modulate a reactivated memory. 4. These results suggest that the time-dependent effects for the modulation of a reactivated memory by fructose (a hexose that does not readily pass the blood-brain barrier) and glucose (a hexose that readily passes the blood-brain barrier) follow similar trends.

Development and validation of the Spanish Hazard Perception Test.

OBJECTIVE: The aim of the current study is to develop and obtain valid evidence for a hazard perception test suitable for the Spanish driving population. To obtain valid evidence to support the use of the test, the effect of hazardous and quasi-hazardous situations on the participants' hazard prediction is analyzed and the pattern of results for drivers with different driving experience--that is, learner, novice, and expert drivers and reoffender vs. nonoffender drivers--is compared. Potentially hazardous situations are those that develop without involving any real hazard (i.e., the driver did not actually have to decelerate or make any evasive maneuver to avoid a potential collision). The current study analyzed repeat offender drivers attending compulsory reeducation programs as a result of reaching the maximum number of penalty points on their driving license due to repeated violations of traffic laws. METHOD: A new video-based hazard perception test was developed, using a total of 20 hazardous situation videos plus 8 quasi-hazardous situation videos. They were selected from 167 recordings of natural hazards in real Spanish driving settings. RESULTS: The test showed adequate psychometric properties and evidence of validity, distinguishing between different types of drivers. Psychometric results confirm a final version of the hazard perception test composed of 11 video clips of hazards and 6 video clips of quasi-hazards, for which an overall Cronbach's alpha coefficient of.77 was obtained. A lack of ability to detect quasi-hazards and distinguish them from hazardous situations was also found for learner, novice, and reoffender drivers. Learner drivers obtained lower average scores than novice and experienced drivers with the hazardous situation videos, and learner drivers obtained lower average scores than experienced drivers with the quasi-hazardous situation videos, suggesting that the ability to correctly identify hazardous traffic situations may develop early by accumulating initial driving experience. However, the ability to correctly identify quasi-hazardous situations may develop later with the accumulation of further driving experience. Developing this ability is also difficult for reoffender drivers. CONCLUSION: The test has adequate psychometric properties and is useful in distinguishing between learner, novice, and expert drivers. In addition, it is useful in that it analyzes the performance of both safe and unsafe drivers (reoffenders who have already lost their driving license).

Validation of dental implant systems through a review of literature supplied by system manufacturers.

STATEMENT OF PROBLEM: The use of endosseous implants to support and retain dental prostheses has become a routine treatment option in many dental practices. Although physical, mechanical, and chemical differences exist among implant systems, survival claims are similar. PURPOSE OF STUDY: This review solicited literature from six implant manufacturers that was thought, by their standards, to be pertinent to the validation of their implant system. METHODS: The literature was reviewed and categorized. CONCLUSION: On the basis of the literature supplied by the manufacturers, only one implant system demonstrated scientifically valid long-term success.

Investigation of adenosine base ionization in the hairpin ribozyme by nucleotide analog interference mapping.

Tertiary structure in globular RNA folds can create local environments that lead to pKa perturbation of specific nucleotide functional groups. To assess the prevalence of functionally relevant adenosine-specific pKa perturbation in RNA structure, we have altered the nucleotide analog interference mapping (NAIM) approach to include a series of a phosphorothioate-tagged adenosine analogs with shifted N1 pKa values. We have used these analogs to analyze the hairpin ribozyme, a small self-cleaving/ligating RNA catalyst that is proposed to employ a general acid-base reaction mechanism. A single adenosine (A10) within the ribozyme active site displayed an interference pattern consistent with a functionally significant base ionization. The exocyclic amino group of a second adenosine (A38) contributes substantially to hairpin catalysis, but ionization of the nucleotide does not appear to be important for activity. Within the hairpin ribozyme crystal structure, A10 and A38 line opposite edges of a solvent-excluded cavity adjacent to the 5'-OH nucleophile. The results are inconsistent with the model of ribozyme chemistry in which A38 acts as a general acid-base catalyst, and suggest that the hairpin ribozyme uses an alternative mechanism to achieve catalytic rate enhancement that utilizes functional groups within a solvent-excluded cleft in the ribozyme active site.