On the Complex and Reversible Pathways of CdSe Quantum Dots Driven by Pyroelectric-Dielectrophoresis.

Electrophoresis (EP) and dielectrophoresis (DEP) are the two well-established methodologies to manipulate nanoparticles (NPs). Recently, DEP by a virtual electrode platform was demonstrated on ferroelectric substrates, where the driving force is due to the strong electric field generated by the pyroelectric effect, thus opening new scenarios for manipulating the matter. Such an innovative approach named pyroelectric-DEP has several advantages over traditional EP and DEP. However, a detailed study on this novel approach is required for understanding the complex pathways traced by NPs under the action of the pyroelectric-driven forces and thus for explaining the final patterns. Here, we investigate experimentally the dynamic behavior of CdSe NPs through time-lapse fluorescence microscopy imaging. Complete visualization and measurement of the directed-assembling process of NPs immersed in polydimethylsiloxane fluid is reported, which shows some unpredicted results with respect to the previous works, thus opening the route for designing in principle a reversible and switchable device allowing two different and reversible final NP-patterned states. The observed phenomena are fully analyzed by experimental and simulated analysis, and the movements of NPs is performed to elucidate in depth the involved processes. The investigation furnishes an interesting result that the complex behavior of the NPs can be fully comprehended and explained by considering the superposition of both EP and DEP forces.

Infrared Imaging Analysis of Green Composite Materials during Inline Quasi-Static Flexural Test: Monitoring by Passive and Active Approaches.

Composite materials have been used for many years in a wide variety of sectors starting from aerospace and nautical up to more commonly used uses such as bicycles, glasses, and so on. The characteristics that have made these materials popular are mainly their low weight, resistance to fatigue, and corrosion. In contrast to the advantages, however, it should be noted that the manufacturing processes of composite materials are not eco-friendly, and their disposal is rather difficult. For these reasons, in recent decades, the use of natural fibers has gained increasing attention, allowing the development of new materials sharing the same advantages with conventional composite systems while respecting the environment. In this work, the behavior of totally eco-friendly composite materials during flexural tests has been studied through infrared (IR) analysis. IR imaging is a well-known non-contact technique and represents a reliable means of providing low-cost in situ analysis. According to this method, the surface of the sample under investigation is monitored, under natural conditions or after heating, by recording thermal images with an appropriate IR camera. Here, the results achieved for jute- and basalt-based eco-friendly composites through the use of both passive and active IR imaging approaches are reported and discussed, showing the possibilities of use also in an industrial environment.

Polypropylene/Basalt Fabric Laminates: Flexural Properties and Impact Damage Behavior.

Recently, the growing interests into the environmental matter are driving the research interest to the development of new eco-sustainable composite materials toward the replacement of synthetic reinforcing fibers with natural ones and exploiting the intrinsic recyclability of thermoplastic resins even for uses in which thermosetting matrices are well consolidated (e.g., naval and aeronautical fields). In this work, polypropylene/basalt fabric composite samples were prepared by film stacking and compression molding procedures. They have been studied in terms of flexural and low-velocity impact behavior. The influence related to the matrix modification with a pre-optimized amount of maleic anhydride grafted PP as coupling agent was studied. The mechanical performances of the composite systems were compared with those of laminates consisting of the pure matrix and obtained by hot-pressing of PP pellets and PP films used in the stacking procedure. Results, on one side, demonstrated a slight reduction of both static and dynamic parameters at the break for specimens from superimposed films to ones prepared from PP pellets. Moreover, an outstanding improvement of mechanical performances was shown in the presence of basalt layers, especially for compatibilized samples.

Compact off-axis holographic slide microscope: design guidelines.

Holographic microscopes are emerging as suitable tools for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through small and compact devices. In-line holographic microscopes can be realized at contained costs, trading off complexity in the phase retrieval process and being limited to sparse samples. Here we present a 3D printed, cost effective and field portable off-axis holographic microscope based on the concept of holographic microfluidic slide. Our scheme removes complexity from the reconstruction process, as phase retrieval is non iterative and obtainable by hologram demodulation. The configuration we introduce ensures flexibility in the definition of the optical scheme, exploitable to realize modular devices with different features. We discuss trade-offs and design rules of thumb to follow for developing DH microscopes based on the proposed solution. Using our prototype, we image flowing marine microalgae, polystyrene beads, E.coli bacteria and microplastics. We detail the effect on the performance and costs of each parameter, design, and hardware choice, guiding readers toward the realization of optimized devices that can be employed out of the lab by non-expert users for point of care testing.

Maskless Arrayed Nanofiber Mats by Bipolar Pyroelectrospinning.

The numerous advantages of micro- and nanostructures produced by electrospinning (ES) have stimulated enormous interest in this technology with potential application in several fields. However, ES still has some limitations in controlling the geometrical arrangement of the fiber mats so that expensive and time-consuming technologies are usually employed for producing ordered geometries. Here we present a technique that we call "bipolar pyroelectrospinning" (b-PES) for generating ordered arrays of fiber mats in a direct manner by using the bipolar pyroelectric field produced by a periodically poled lithium niobate crystal (PPLN). The b-PES is free from expensive electrodes, nozzles, and masks because it makes use simply of the structured pyroelectric field produced by the PPLN crystal used as collector. The results show clearly the reliability of the technique in producing a wide variety of arrayed fiber mats that could find application in bioengineering or many other fields. Preliminary results of live cells patterning under controlled geometrical constraints is also reported and discussed in order to show potential exploitation as a scaffold in tissue engineering.

Pyro-Electrification of Freestanding Polymer Sheets: A New Tool for Cation-Free Manipulation of Cell Adhesion in vitro.

Localized electric fields have become, in recent years, a source of inspiration to researchers and laboratories thanks to a huge amount of applications derived from it, including positioning of microparticles as building blocks for electrical, optical, and magnetic devices. The possibility of producing polymeric materials with surface charge thus opens new perspectives for applications where process simplicity and cost-effectiveness of flexible electronics are of fundamental importance. In particular, the influence of surface charges is widely studied and is a critical issue especially when new materials and functional technologies are introduced. Here, we report a voltage-free pyro-electrification (PE) process able to induce a permanent dipole orientation into polymer sheets under both mono- and bipolar distribution. The technique makes use of the pyroelectric effect for generating electric potentials on the order of kilovolts by an easy-to-accomplish thermal treatment of ferroelectric lithium niobate (LN) crystals. The PE allows us to avoid the expensive and time-consuming fabrication of high-power electrical circuits, as occurs in traditional generator-based techniques. Since the technique is fully compatible with spin-coating-based procedures, the pyro-electrified polymer sheets are easily peeled off the surface of the LN crystal after PE completion, thus providing highly stable and freestanding charged sheets. We show the reliability of the technique for different polymers and for different applications ranging from live cell patterning to biofilm formation tests for bacteria linked to food-processing environments.

Endowing a plain fluidic chip with micro-optics: a holographic microscope slide.

Lab-on-a-Chip (LoC) devices are extremely promising in that they enable diagnostic functions at the point-of-care. Within this scope, an important goal is to design imaging schemes that can be used out of the laboratory. In this paper, we introduce and test a pocket holographic slide that allows digital holography microscopy to be performed without an interferometer setup. Instead, a commercial off-the-shelf plastic chip is engineered and functionalized with this aim. The microfluidic chip is endowed with micro-optics, that is, a diffraction grating and polymeric lenses, to build an interferometer directly on the chip, avoiding the need for a reference arm and external bulky optical components. Thanks to the single-beam scheme, the system is completely integrated and robust against vibrations, sharing the useful features of any common path interferometer. Hence, it becomes possible to bring holographic functionalities out of the lab, moving complexity from the external optical apparatus to the chip itself. Label-free imaging and quantitative phase contrast mapping of live samples are demonstrated, along with flexible refocusing capabilities. Thus, a liquid volume can be analyzed in one single shot with no need for mechanical scanning systems.

Bipolar Patterning of Polymer Membranes by Pyroelectrification.

Polymer freestanding membranes with permanent bipolar patterns are fabricated by "pyroelectrification". The thermal stimulation of periodically poled lithium niobate (PPLN) crystals simultaneously generates the pyroelectric effect, the glass transition of the polymer, and therefore the periodic electric poling of the polymer. The reliability of these membranes is demonstrated for applications under both dry and wet conditions, including cell patterning.

Reversible Holographic Patterns on Azopolymers for Guiding Cell Adhesion and Orientation.

Topography of material surfaces is known to influence cell behavior at different levels: from adhesion up to differentiation. Different micro- and nanopatterning techniques have been employed to create patterned surfaces to investigate various aspects of cell behavior, most notably cellular mechanotransduction. Nevertheless, conventional techniques, once implemented on a specific substrate, fail in allowing dynamic changes of the topographic features. Here we investigated the response of NIH-3T3 cells to reversible topographic signals encoded on light-responsive azopolymer films. Switchable patterns were fabricated by means of a well-established holographic setup. Surface relief gratings were realized with Lloyd's mirror system and erased with circularly polarized or incoherent light. Cell cytoskeleton organization and focal adhesion assembly proved to be very sensitive to the underlying topographic signal. Thereafter, pattern reversibility was tested in air and wet environment by using temperature or light as a trigger. Additionally, pattern modification was dynamically performed on substrates with living cells. This study paves the way toward an in situ and real-time investigation of the material-cytoskeleton crosstalk caused by the intrinsic properties of azopolymers.