Ancient DNA sheds light on the ancestry of pre-hispanic Canarian pigs.

BACKGROUND: Canarian Black (CB) pigs belong to an autochthonous and endangered breed, which is spread throughout the Canarian archipelago. It is commonly accepted that they represent a relic of the pig populations that were bred by the Berbers in North Africa over millennia. It is important to note that the geographic isolation of the Canary Islands has preserved this genetic legacy intact from foreign introgressions until the Spanish conquest of the archipelago in the 15(th) century. Ten years ago, it was demonstrated that, in CB pigs, the frequency of the Asian A2 cytochrome-b haplogroup reached 73%. The current work aimed at investigating whether this observation is explained by either a recent or an ancient introgression of CB pigs with Far Eastern pigs. RESULTS: Genetic analyses of 23 ancient samples from pre-hispanic Canarian pigs (420 to 2500 years before present) showed that Near Eastern and Far Eastern genetic signatures were totally absent in the primitive Canarian pre-hispanic pigs. Indeed, the haplotypes detected in these pigs were closely related to those of North African and European wild boars. CONCLUSIONS: Our results demonstrate that the high frequency of the Far Eastern mitochondrial cytochrome B A2 haplotype in modern Canarian Black pigs probably corresponds to a relatively recent introgression with British breeds.

The genomic history of the indigenous people of the Canary Islands.

The indigenous population of the Canary Islands, which colonized the archipelago around the 3rd century CE, provides both a window into the past of North Africa and a unique model to explore the effects of insularity. We generate genome-wide data from 40 individuals from the seven islands, dated between the 3rd-16rd centuries CE. Along with components already present in Moroccan Neolithic populations, the Canarian natives show signatures related to Bronze Age expansions in Eurasia and trans-Saharan migrations. The lack of gene flow between islands and constant or decreasing effective population sizes suggest that populations were isolated. While some island populations maintained relatively high genetic diversity, with the only detected bottleneck coinciding with the colonization time, other islands with fewer natural resources show the effects of insularity and isolation. Finally, consistent genetic differentiation between eastern and western islands points to a more complex colonization process than previously thought.

Performance of Asphalt Rubber Mixture Overlays to Mitigate Reflective Cracking.

Adequately predicting overlay behaviour is essential for flexible pavement rehabilitation to reach the predicted lifespan. Reflective cracking is one of the main failure mechanisms affecting overlay performance. This failure may occur due to cracks in the lower layers that propagate to the overlay due to traffic loads, temperature variations, shrinkage cracking of cement-treated layers, and subgrade movements. This work aims to assess the reflective cracking phenomenon of asphalt rubber mixtures as an overlay through laboratory tests and numerical simulation. Four-point bending equipment and the reflective crack device were used to perform the laboratory tests. A numerical simulation through the finite element method was accomplished to estimate the von Mises strain and develop reflective cracking fatigue laws. The results showed that the asphalt rubber mixtures are suitable for extending overlay lifespan considering reflective cracking. The evaluated asphalt rubber mixtures presented reflective cracking resistance almost eight times greater than the conventional ones.

Contribution of Asphalt Rubber Mixtures to Sustainable Pavements by Reducing Pavement Thickness.

Asphalt rubber mixtures have been used as pavement surface layers due to their ability to prevent early degradation, and are considered a sustainable option. This study performed analysis comparing the fatigue resistance of asphalt rubber mixtures of different combinations of asphalt bases, crumb rubber, and gradation, in order to estimate the performance of asphalt rubber mixtures as pavement surface layers. The methodology was developed in a laboratory and involved asphalt rubber production by continuous and terminal blend systems with different crumb rubbers and asphalt base types. Asphalt rubber mixtures with varying gradations and an unmodified asphalt mixture as a reference were produced. The mechanical behavior as a dynamic modulus and with respect to fatigue resistance was evaluated using a four-point bending test. In order to verify each of the asphalt rubber mixtures' contribution as a surface layer, pavement structures were designed and their lifespans were compared. The findings showed that all asphalt rubber mixtures presented higher fatigue resistance than the reference. For pavement design, in comparison with the reference mixture, the thickness of the surface layer could be reduced by at least 50% while achieving the same life, proving its successful performance. This study demonstrated the effective contribution of crumb rubber from scrap tires as an asphalt modifier for producing sustainable mixtures with adequate fatigue performance.

Mitogenomes illuminate the origin and migration patterns of the indigenous people of the Canary Islands.

The Canary Islands' indigenous people have been the subject of substantial archaeological, anthropological, linguistic and genetic research pointing to a most probable North African Berber source. However, neither agreement about the exact point of origin nor a model for the indigenous colonization of the islands has been established. To shed light on these questions, we analyzed 48 ancient mitogenomes from 25 archaeological sites from the seven main islands. Most lineages observed in the ancient samples have a Mediterranean distribution, and belong to lineages associated with the Neolithic expansion in the Near East and Europe (T2c, J2a, X3a…). This phylogeographic analysis of Canarian ancient mitogenomes, the first of its kind, shows that some lineages are restricted to Central North Africa (H1cf, J2a2d and T2c1d3), while others have a wider distribution, including both West and Central North Africa, and, in some cases, Europe and the Near East (U6a1a1, U6a7a1, U6b, X3a, U6c1). In addition, we identify four new Canarian-specific lineages (H1e1a9, H4a1e, J2a2d1a and L3b1a12) whose coalescence dates correlate with the estimated time for the colonization of the islands (1st millennia CE). Additionally, we observe an asymmetrical distribution of mtDNA haplogroups in the ancient population, with certain haplogroups appearing more frequently in the islands closer to the continent. This reinforces results based on modern mtDNA and Y-chromosome data, and archaeological evidence suggesting the existence of two distinct migrations. Comparisons between insular populations show that some populations had high genetic diversity, while others were probably affected by genetic drift and/or bottlenecks. In spite of observing interinsular differences in the survival of indigenous lineages, modern populations, with the sole exception of La Gomera, are homogenous across the islands, supporting the theory of extensive human mobility after the European conquest.