RESUMEN
Shanghai is one of the largest cities in China and has the highest proportion of inbound travelers in the world. The HIV (human immunodeficiency virus) infection status of this population can reflect the global HIV epidemic trend to a certain extent. Therefore, we conducted a retrospective epidemic survey to clarify the prevalence and characteristics of HIV-1 infection among inbound travelers applying for Shanghai residency. The retrospective cohort analysis included a total of 50,830 inbound tourists/travelers who applied for Shanghai residency from 2005 to 2016. HIV-1 infection rates were determined based on HIV-1 antibody testing. Among all the recruited inbound travelers, 245 were HIV-1 positive, with an infection rate of 0.48%. The detection rate of HIV in males was significantly higher than in females (χ2 = 62.584, P < 0.0001). Those aged 18-30 years, 31-40 years, and >40 years accounted for 34.3%, 39.6%, and 26.1% of the infected population, respectively. Although the annual detection rates were different, the trend analysis results showed no increase in HIV-1 prevalence rates among the sampling years (Cochran-Armitage Z = 2.543, P = 0.111). Proportions of individuals infected through homosexual transmission increased over the study period (Cochran-Armitage Z = 5.41, P < 0.001), while the proportion infected through heterosexual declined over time (Cochran-Armitage Z = 3.38, P = 0.001). The rate and characteristics of HIV-1 infection among foreign applicants for residency in Shanghai were identified in the study. The results provide the necessary epidemiological data for monitoring the HIV-1 epidemic among international entry travelers and contribute to establishing relevant policies and regulations for HIV control and prevention.
RESUMEN
BACKGROUND: Vaginitis is a common and very private disease, and the current diagnosis is a frequent go to the hospital for testing. OBJECTIVE: In order to improve the convenience and speed of detection, in this paper, we have developed a hand-held zoom micro-imaging system based on a microfluidic chip for point-of-care testing (POCT) of vaginal inflammation. METHODS: This system consists of a microfluidic chip, an optical system and a hand-held zoom system assembled with a mobile phone. In terms of hardware, we designed a self-priming microfluidic chip, which can realize automatic sampling and full mixing of samples. We have also developed an optical system that can be adapted to smartphones, which has a lens group with a 37x magnification function and equipped with a zoom system with a focus range of 4mm to 6mm. In terms of software, we proposed an APP that can accurately identify cocci and can determine the inflammation level. RESULTS: Compared with the recognition rate of the observers in the hospital, the detection accuracy of the portable recognition system is 95%, and after testing the clinical samples, the results were completely consistent with the hospital diagnosis results. The detection limit was 500 CFU / ml, which the relative error was (0.9 ± 0.3) %, and recognition time is 7 seconds. CONCLUSION: This system is definitely suitable for women's point-of-care testing (POCT).