RESUMEN
SUMMARY: *Owing to nitrogen (N) translocation towards new leaves, the shedding of old leaves can increase the whole-plant carbon gain. It occurs when their photosynthetic nitrogen use efficiency (PNUE) declines below a given threshold. *Here, we investigated variations in net photosynthetic capacity (A(max)), N resorption and PNUE in populations of Rhododendron ferrugineum presenting different mean leaf life spans (LLS). *Both populations had comparable annual leaf surface area production and A(max) across leaf-age cohorts. Branch photosynthetic capacity was up to 95% higher in the population with the longer LLS mainly because of the high contribution of old leaves to the total leaf area. Despite lower N concentrations, old leaves maintained relatively high A(max) and consequently PNUE that were higher than or similar to the values found in current-year leaves. *As the ratio of PNUE in old to PNUE in new leaves was always higher than the fraction of leaf N resorbed during leaf shedding, we concluded that leaf shedding did not improve plant photosynthetic capacity. We suggest that in R. ferrugineum, leaf shedding is mainly controlled by the leaf storage function and, therefore, that models aiming to explain LLS should not only consider the leaf carbon assimilation function, particularly in nutrient-poor habitats.
Asunto(s)
Biomasa , Fotosíntesis/fisiología , Hojas de la Planta/crecimiento & desarrollo , Rhododendron/crecimiento & desarrollo , Carbono/metabolismo , Respiración de la Célula , Oscuridad , Gases/metabolismo , Nitrógeno/metabolismo , Hojas de la Planta/anatomía & histología , Hojas de la Planta/citología , Rhododendron/citología , Rhododendron/metabolismo , Propiedades de Superficie , Factores de TiempoRESUMEN
How the balance between exogenous and endogenous nitrogen for shoot growth varies with soil nitrogen availability, and its consequences on leaf life-span, have rarely been studied within a single species in the field. In this study, we investigated two Rhododendron ferrugineum populations with contrasting leaf life-span. Soil nitrogen availability and nitrogen resorption of different leaf age classes were assessed, as were the interactions between plant compartments, using (15)N labelling and sink organ suppression. The population growing on poorer soil had a shorter leaf life-span (17.9 vs 21.5 months) and a higher net contribution of leaf reserves to shoot growth (32% vs 15%), achieved by faster nitrogen resorption and greater shedding of young nitrogen-rich leaves. For both populations, wood contributed to over 40% of shoot nitrogen demand. Both the negative relationship between current-year shoot mass and the percentage of 1-yr-old attached leaves and the delay of leaf shedding after bud removal suggest that shoot development has a strong effect on leaf life-span. Our results suggest that, contrary to the evolutionary response, plastic response to low soil nitrogen could reduce leaf life-span in evergreen plants. In addition, leaf life-span seems to be strongly influenced by the discrepancy between shoot nitrogen demand and soil nitrogen uptake rather than nitrogen demand alone.
Asunto(s)
Senescencia Celular/fisiología , Nitrógeno/metabolismo , Hojas de la Planta/metabolismo , Brotes de la Planta/metabolismo , Rhododendron/metabolismo , Suelo , Transporte Biológico/fisiología , Hojas de la Planta/crecimiento & desarrollo , Brotes de la Planta/crecimiento & desarrollo , Rhododendron/crecimiento & desarrollo , Madera/metabolismoRESUMEN
Plant mating systems are driven by several pre-pollination factors, including pollinator availability, mate availability and reproductive traits. We investigated the relative contributions of these factors to pollination and to realized outcrossing rates in the patchily distributed mass-flowering shrub Rhododendron ferrugineum. We jointly monitored pollen limitation (comparing seed set from intact and pollen-supplemented flowers), reproductive traits (herkogamy, flower size and autofertility) and mating patterns (progeny array analysis) in 28 natural patches varying in the level of pollinator availability (flower visitation rates) and of mate availability (patch floral display estimated as the total number of inflorescences per patch). Our results showed that patch floral display was the strongest determinant of pollination and of the realized outcrossing rates in this mass-flowering species. We found an increase in pollen limitation and in outcrossing rates with increasing patch floral display. Reproductive traits were not significantly related to patch floral display, while autofertility was negatively correlated to outcrossing rates. These findings suggest that mate limitation, arising from high flower visitation rates in small plant patches, resulted in low pollen limitation and high selfing rates, while pollinator limitation, arising from low flower visitation rates in large plant patches, resulted in higher pollen limitation and outcrossing rates. Pollinator-mediated selfing and geitonogamy likely alleviates pollen limitation in the case of reduced mate availability, while reduced pollinator availability (intraspecific competition for pollinator services) may result in the maintenance of high outcrossing rates despite reduced seed production.
Asunto(s)
Insectos/fisiología , Polinización/fisiología , Rhododendron/fisiología , Animales , Cruzamientos Genéticos , Flores/genética , Flores/fisiología , Análisis Multivariante , Fenotipo , Polen/genética , Polen/fisiología , Reproducción/fisiología , Rhododendron/genética , Semillas/genética , Semillas/fisiologíaRESUMEN
⢠Internal cycling of nitrogen (N) was investigated in a subalpine field population of the evergreen shrub Rhododendron ferrugineum during spring growth. ⢠The foliar nitrogen of 5-yr-old-plants was directly labeled with 15 N and subsequently traced to all plant compartments. In addition, 15 N-ammonium uptake was estimated in glasshouse experiments. ⢠Before shoot growth, redistribution of 15 N occurred in the plant without net N transfer. During spring development, the decreases in both leaf 15 N and total N were almost identical in terms of percentage, and most of the 15 N withdrawn from the leaf compartments was recovered in the growing shoots. Net changes in the N contents of the various leaf and woody compartments indicate that internal remobilization (especially from 1-yr-old leaves) could have met most of the N needs of new shoot growth. Simultaneously, the rate of mineral N uptake was very low. ⢠Thus, leaves in young plants provide N for new shoots (by contrast with old individuals) and allow, with woody tissues, almost complete uncoupling of N requirement for spring growth from root uptake.
RESUMEN
Fragmentation of natural vegetation creates one of the largest threats to plant-pollinator interactions. Although fragmentation impacts on plant populations have been explored in many, mainly herbaceous, species, the response of wild mass-flowering species is poorly known. Here, we studied 28 heathland patches dominated by the mass-flowering shrub Rhododendron ferrugineum, each presenting different R. ferrugineum floral display sizes (total inflorescence number per patch) and patch isolation (median distance to the three nearest patches). We assessed the impacts of these two factors on (i) heathland patch visitor assemblage (considering R. ferrugineum versus surrounding community) and (ii) R. ferrugineum flower visitation rate and pollen transfer limitation (comparing seed set from emasculated to pollen-supplemented flowers). We found that diversity and abundance of bees visiting R. ferrugineum in heathland patches significantly decreased with decreasing R. ferrugineum floral display, while overall visitor density per patch and flower visitation rate increased. Moreover, a decrease in massive floral display and increase in patch isolation resulted in reduced visitor density in the surrounding community. Even in patches with few individuals, we found disproportionate visitor abundance in R. ferrugineum compared to the surrounding community. Finally, pollen transfer limitation in R. ferrugineum was neither affected by visitation rate nor by patch attributes. By disproportionally attracting pollinators from co-flowering species, and probably promoting geitonogamous pollen transfer, the mass-flowering trait appears adequate to compensate, in terms of conspecific pollen transfer, for the decrease in visitor diversity and abundance and in mate availability, which usually result from population fragmentation.
Asunto(s)
Flores , Insectos/fisiología , Polen , Polinización , Rhododendron/fisiología , AnimalesRESUMEN
Here, the advantages for a shrub of having long vs short-lived leaves was investigated in Rhododendron ferrugineum by following nitrogen(15N) and carbon(14C) resorption and translocation, and photosynthetic capacity over the life span. Mean leaf life span was 19 months. Nitrogen (N) resorption in attached leaves occurred mainly in the first year (23%) and reached a maximum of 31% in the second. Although, resorption was similar in attached and fallen 1-yr-old leaves, it was on average one-third higher in fallen than in attached older leaves. Final N resorption of a leaf compartment reached 41%, half occurring from healthy leaves during the first year. Photosynthetic capacity decreased slightly during the life span. Before shoot growth, plant photosynthesis was mainly supported by 1-yr-old leaves, although the contribution of the 2-yr-old leaves was nonnegligible (15% of the capacity and higher carbon transfer toward the roots). After shoot growth, the current-year leaves made the greatest contribution. Our results suggest that short-lived leaves (half of the cohort) are mainly involved in a photosynthetic function, having a high photosynthetic capacity and drawing most of their resorbed N towards current-year leaves; and long-lived leaves are also involved in a conservative function, increasing N resorption and mean residence time (MRT).
Asunto(s)
Nitrógeno/metabolismo , Fotosíntesis/fisiología , Hojas de la Planta/fisiología , Rhododendron/metabolismo , Biomasa , Ecosistema , Isótopos de Nitrógeno , Raíces de Plantas/metabolismo , Tallos de la Planta/metabolismo , Factores de TiempoRESUMEN
Two populations of Rhododendron ferrugineum growing at subalpine level in the Pyrenees (France) were studied in two sites (Bethmale and Mourtis). Identification and delimitation of genets were inferred from amplified fragment length polymorphism (AFLP) markers, along a closure gradient (from meadow to more closed heath) in each site. Surface and age of genets, genotypic diversity (Simpson's index D), 'proportion distinguishable' genotypes and genetic relationships between genets were then estimated. Amplification of the 312 DNA samples with three selective primer pairs gave a mean of 98 detectable peaks (i.e. bands) per sample, with size ranging from 60 to 300 bp. In total 60% (Bethmale) and 70% (Mourtis) of the peaks were polymorphic, and a total of 31 and 23 multilocus genotypes were identified, in Bethmale and Mourtis, respectively. We inferred that pioneer genotypes began arriving 110 years ago mainly over a 40-year period in the Mourtis meadow, and began about 130 years ago over a 100-year period in the Bethmale meadow. After this pioneer stage, populations extended vegetatively. Two different patterns of genotypic dynamics can be identified. At Bethmale, population closure could have led to a dramatic loss of genets and to the selection of highly genetically related genotypes. In contrast, at Mourtis, genotypic diversity and genet density did not change fundamentally along the closure gradient. However the range of genetic diversity diminished from the open to the closed situation, suggesting that thinning could have occurred in the past.
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Magnoliopsida/genética , Polimorfismo Genético , Ecología , Francia , Genotipo , Geografía , Selección GenéticaRESUMEN
We investigated the effect of c-Fos and/or c-Jun co-expression on transcription activation by the progesterone (PR), glucocorticoid (GR) or androgen (AR) receptors using three different reporter genes and four different cell lines. We found that c-Fos could only inhibit, while c-Jun could either inhibit or further stimulate receptor-induced transcription. All these effects were receptor, promoter, and cell type specific, and, importantly, the steroid receptors had non-reciprocal effects on the transactivation ability of c-Jun in the presence or absence of c-Fos. Collectively, these results argue against heterodimer formation as a mechanism to explain the phenomena. Transactivation by the endogenous PR in T47D cells could be inhibited by increasing the intracellular c-Fos level with forskolin as well as by co-expressing c-Fos; no such effect was seen in MCF-7 cells. The inhibition by c-Fos of PR-induced transcription involves a competitive mechanism, which requires the presence of the intact c-Fos leucine zipper and is directed mainly at the transcription activation function (TAF) located in the PR and GR hormone binding domains (TAF-2). However, the co-expression of c-Fos did not alter the 'squelching/transcriptional interference' by the PR of estrogen receptor (ER)-induced transcription. Multiple mechanisms are discussed which may be involved in the crosstalk between the two signal transduction pathways.
Asunto(s)
Proteínas Proto-Oncogénicas c-fos/genética , Proteínas Proto-Oncogénicas c-jun/genética , Receptores de Esteroides/metabolismo , Activación Transcripcional , Secuencia de Bases , Cloranfenicol O-Acetiltransferasa/metabolismo , Colforsina/farmacología , ADN , Células HeLa , Humanos , Leucina Zippers , Datos de Secuencia Molecular , Mutación , Plásmidos , Regiones Promotoras Genéticas , Proteínas Proto-Oncogénicas c-fos/farmacología , Proteínas Proto-Oncogénicas c-jun/farmacología , Receptores Androgénicos/efectos de los fármacos , Receptores Androgénicos/metabolismo , Receptores de Glucocorticoides/efectos de los fármacos , Receptores de Glucocorticoides/metabolismo , Receptores de Progesterona/efectos de los fármacos , Receptores de Progesterona/metabolismo , Receptores de Esteroides/efectos de los fármacos , Transcripción Genética , TransfecciónRESUMEN
RU486 induced the binding to a palindromic progestin responsive element (PRE) in vitro of homo- and heterodimers of the human progesterone receptor (hPR) isoforms A and B, present in T47D breast cancer cells or in HeLa cells transiently expressing the recombinant proteins. The resulting complexes were indistinguishable from those induced with the agonist R5020 with respect to specificity, affinity and stability. Ligand exposure was a necessary prerequisite to observe PR/PRE complexes. Antagonist-induced complexes migrated more rapidly during electrophoresis than agonist-induced ones, and no 'mixed' PR/RU486-PR/R5020 complexes were observed, suggesting that the dimerization interfaces of agonist- and antagonist-bound molecules are non-compatible. The analysis of a series of deletion mutants and chimeric receptors revealed the presence of two transcription activation functions (TAFs), located in the N-terminal region A/B (TAF-1) and the hormone binding domain (TAF-2). In the presence of agonists, both TAFs were active in HeLa cells. In the presence of RU486 TAF-2 was inactive, while TAF-1 within the hPR form B/RU486 complex activated transcription from a reporter gene containing a single palindromic PRE. We consider this to be the most convincing evidence that the receptor/RU486-complex does in fact bind to PREs in vivo. No transcriptional activation was observed in the presence of RU486 from a reporter gene containing the complex MMTV-LTR PRE. In contrast to hPR form B, form A was not able to activate transcription from PRE/GRE-tk-CAT in the presence of RU486. In vivo competition between hPR/RU486 and either cPR/R5020 or the human glucocorticoid receptor/dexamethasone (hGR/Dex) complex further supported that hPR/RU486 bound in vivo to its cognate responsive element. Indeed, the observed inhibition of transcription was shown to be due to competition for the MMTV PRE, since no transcriptional interference by the hPR/RU486 was observed, and since no heterodimers were formed between hPR/RU486 and cPR/R5020 or hGR/Dex. That the ligand-free hPR, however, was unable to compete, demonstrated that ligand binding is the prerequisite for DNA binding of hPR in vivo.
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Mifepristona/farmacología , Progesterona/metabolismo , Receptores de Progesterona/fisiología , Animales , Sitios de Unión , Neoplasias de la Mama , Línea Celular , Pollos , Deleción Cromosómica , Femenino , Regulación de la Expresión Génica , Células HeLa/metabolismo , Humanos , Mifepristona/metabolismo , Mutagénesis Sitio-Dirigida , Regiones Promotoras Genéticas , Receptores de Progesterona/efectos de los fármacos , Receptores de Progesterona/genética , Transcripción Genética , TransfecciónRESUMEN
4-[5H-2,3-(2,5-Dimethyl-2,5-hexano)-5-methyldibenzo[b,e][1,4 ]diazepin-11-yl]benzoic acid (HX600), as well as its oxa- (HX620) and thia- (HX630) analogs, enhanced the activity of retinoic acid and a receptor alpha (RAR alpha)-selective agonist Am80 in HL-60 cell differentiation assays. HX600 synergizes with Am80 by binding to, and transactivating through, the RXR subunit of the RXR-RAR heterodimer. HX600 exhibited RXR pan-agonist activity in transient transfections with a DR1-based reporter gene and synergized with RA-bound RAR alpha and RAR beta in inducing transcription from a DR5-based reporter. In addition, all three compounds at high concentrations acted as RAR pan-antagonists in stably transfected RAR "reporter cells." These efficient synergists bind only weakly with RXRs in vitro, suggesting that they are RXR-RAR heterodimer-selective activators. These HX retinoids exhibited dual functionality, since they affected signalling through both retinoid receptor families (RARs and RXRs).