BCG vaccination stimulates integrated organ immunity by feedback of the adaptive immune response to imprint prolonged innate antiviral resistance.

Bacille Calmette-Guérin (BCG) vaccination can confer nonspecific protection against heterologous pathogens. However, the underlying mechanisms remain mysterious. We show that mice vaccinated intravenously with BCG exhibited reduced weight loss and/or improved viral clearance when challenged with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 B.1.351) or PR8 influenza. Protection was first evident between 14 and 21 d post-vaccination and lasted ∼3 months. Notably, BCG induced a biphasic innate response and robust antigen-specific type 1 helper T cell (TH1 cell) responses in the lungs. MyD88 signaling was essential for innate and TH1 cell responses, and protection against SARS-CoV-2. Depletion of CD4+ T cells or interferon (IFN)-γ activity before infection obliterated innate activation and protection. Single-cell and spatial transcriptomics revealed CD4-dependent expression of IFN-stimulated genes in lung myeloid and epithelial cells. Notably, BCG also induced protection against weight loss after mouse-adapted SARS-CoV-2 BA.5, SARS-CoV and SHC014 coronavirus infections. Thus, BCG elicits integrated organ immunity, where CD4+ T cells feed back on tissue myeloid and epithelial cells to imprint prolonged and broad innate antiviral resistance.

Non-neutralizing SARS-CoV-2 N-terminal domain antibodies protect mice against severe disease using Fc-mediated effector functions.

Antibodies perform both neutralizing and non-neutralizing effector functions that protect against certain pathogen-induced diseases. A human antibody directed at the SARS-CoV-2 Spike N-terminal domain (NTD), DH1052, was recently shown to be non-neutralizing, yet it protected mice and cynomolgus macaques from severe disease. The mechanisms of NTD non-neutralizing antibody-mediated protection are unknown. Here we show that Fc effector functions mediate NTD non-neutralizing antibody (non-nAb) protection against SARS-CoV-2 MA10 viral challenge in mice. Though non-nAb prophylactic infusion did not suppress infectious viral titers in the lung as potently as neutralizing antibody (nAb) infusion, disease markers including gross lung discoloration were similar in nAb and non-nAb groups. Fc functional knockout substitutions abolished non-nAb protection and increased viral titers in the nAb group. Fc enhancement increased non-nAb protection relative to WT, supporting a positive association between Fc functionality and degree of protection from SARS-CoV-2 infection. For therapeutic administration of antibodies, non-nAb effector functions contributed to virus suppression and lessening of lung discoloration, but the presence of neutralization was required for optimal protection from disease. This study demonstrates that non-nAbs can utilize Fc-mediated mechanisms to lower viral load and prevent lung damage due to coronavirus infection.

Effects of experimental warming on floral scent, display, and rewards in two subalpine herbs.

BACKGROUND AND AIMS: Floral volatiles, visual traits, and rewards mediate attraction and defense in plant-pollinator and plant-herbivore interactions, but these floral traits may be altered by global warming through direct effects of temperature or longer term impacts on plant resources. We examined the effect of warming on floral and leaf volatile emissions, floral morphology, plant height, nectar production, and oviposition by seed predators. METHODS: We used open-top chambers that warmed plants in the field +2-3 °C on average (+6-11 °C increase in daily maxima) for 2-4 weeks across 1-3 years at 3 sites in Colorado, USA. Volatiles were sampled from two closely related species of subalpine Ipomopsis with different pollinators: I. aggregata ssp. aggregata, visited mainly by hummingbirds, and I. tenuituba ssp. tenuituba, often visited by hawkmoths. KEY RESULTS: While warming had no detected effects on leaf volatiles, the daytime floral volatiles of both I. aggregata and I. tenuituba responded in subtle ways to warming, with impacts that depended on the species, site, and year. In addition to the long-term effect of warming, temperature at the time of sampling independently affected the floral volatile emissions of I. aggregata during the day and I. tenuituba at night. Warming had little effect on floral morphology for either species, and no effect on nectar concentration, maximum inflorescence height, or flower redness in I. aggregata. However, warming increased nectar production in I. aggregata by 41%, a response that would attract more hummingbird visits, and reduced oviposition by fly seed predators by at least 72%. CONCLUSIONS: Our results suggest that floral traits can show different levels of plasticity to temperature changes in subalpine environments, with potential effects on animal behaviors that help or hinder plant reproduction. They also illustrate the need for more long-term field warming studies, as shown by responses of floral volatiles in different ways to weeks of warming versus temperature at the time of sampling.

Translucency and flexural strength of translucent zirconia ceramics.

STATEMENT OF PROBLEM: Translucent zirconias have been developed with better esthetics than high-strength zirconias by reducing opacity. However, studies on their translucency and strength are sparse. PURPOSE: The purpose of this in vitro study was to compare the relationship between translucency and biaxial flexural strength of recently developed high-translucency zirconia, high-strength zirconia, and lithium disilicate ceramics. MATERIAL AND METHODS: Disks (n=12) were fabricated for 5 ceramic materials: high-strength zirconia (BruxZir 16 shaded), translucent zirconia (BruxZir Anterior shaded, Katana UTML, Katana STML), and lithium disilicate (IPS e.max, Press HT, and LT). A standard tessellation language (STL) file was designed, and the specimen milled, finished, and glazed according to manufacturer's instructions for each material. The translucency parameter was calculated against black and white backgrounds and white and stump shade with ND4 background by using a spectrophotometer. Biaxial flexural strength was calculated by using the 3-ball test. The load was applied at a crosshead speed of 0.5 mm/min with a 49-N load cell until failure occurred. Translucency parameter and biaxial flexural strength data were analyzed with 1-way analysis of variance (ANOVA). A Tukey honest significant difference multiple comparison test was used to determine significant differences (α=.05). RESULTS: The IPS e.max HT was more translucent against both backgrounds (32.85 for black/white and 15.34 for white/stump), while BruxZir 16 was the least translucent (19.78 for B/W and 8.83 for W/S). All groups tested differed in translucency (P<.001) except for BruxZir Anterior and Katana STML, which were not significantly different (P=.052). For biaxial flexural strength, BruxZir 16 had the highest strength (995.44 MPa) and e.max HT, the lowest (186.75 MPa). No significant differences were found between BruxZir anterior and Katana STML, Katana UTML and IPS e.max LT, or IPS e.max LT and IPS e.max HT (P>.05). Translucency parameter values using both backgrounds were strongly correlated (r=0.99). However, biaxial flexural strength values were inversely related to translucency parameter values when using black/white and white/stump shade but with high correlation (r=-0.777 and -0.756 respectively). CONCLUSIONS: Lithium disilicate was the most translucent and yet the weakest material, whereas high-strength zirconia was the most opaque ceramic and the strongest. Katana UTML had the highest translucency but was weakest among translucent and high-strength zirconia materials. Overall, translucency was negatively correlated with biaxial flexural strength.

Phenotypic plasticity and selection on leaf traits in response to snowmelt timing and summer precipitation.

Vegetative traits of plants can respond directly to changes in the environment, such as those occurring under climate change. That phenotypic plasticity could be adaptive, maladaptive, or neutral. We manipulated the timing of spring snowmelt and amount of summer precipitation in factorial combination and examined responses of specific leaf area (SLA), trichome density, leaf water content (LWC), photosynthetic rate, stomatal conductance and intrinsic water-use efficiency (iWUE) in the subalpine herb Ipomopsis aggregata. The experiment was repeated in three years differing in natural timing of snowmelt. To examine natural selection, we used survival, relative growth rate, and flowering as fitness indices. A 50% reduction in summer precipitation reduced stomatal conductance and increased iWUE, and doubled precipitation increased LWC. Combining natural and experimental variation, earlier snowmelt reduced soil moisture, photosynthetic rate and stomatal conductance, and increased trichome density and iWUE. Precipitation reduction reversed the mortality selection favoring high stomatal conductance under normal and doubled precipitation, and higher LWC improved growth. Earlier snowmelt is a strong signal of climate change and can change expression of leaf morphology and gas exchange traits, just as reduced precipitation can. Stomatal conductance and SLA showed adaptive plasticity under some conditions.

Earlier snow melt and reduced summer precipitation alter floral traits important to pollination.

Climate change can cause changes in expression of organismal traits that influence fitness. In flowering plants, floral traits can respond to drought, and that phenotypic plasticity has the potential to affect pollination and plant reproductive success. Global climate change is leading to earlier snow melt in snow-dominated ecosystems as well as affecting precipitation during the growing season, but the effects of snow melt timing on floral morphology and rewards remain unknown. We conducted crossed manipulations of spring snow melt timing (early vs. control) and summer monsoon precipitation (addition, control, and reduction) that mimicked recent natural variation, and examined plastic responses in floral traits of Ipomopsis aggregata over 3 years in the Rocky Mountains. We tested whether increased summer precipitation compensated for earlier snow melt, and if plasticity was associated with changes in soil moisture and/or leaf gas exchange. Lower summer precipitation decreased corolla length, style length, corolla width, sepal width, and nectar production, and increased nectar concentration. Earlier snow melt (taking into account natural and experimental variation) had the same effects on those traits and decreased inflorescence height. The effect of reduced summer precipitation was stronger in earlier snow melt years for corolla length and sepal width. Trait reductions were explained by drier soil during the flowering period, but this effect was only partially explained by how drier soils affected plant water stress, as measured by leaf gas exchange. We predicted the effects of plastic trait changes on pollinator visitation rates, pollination success, and seed production using prior studies on I. aggregata. The largest predicted effect of drier soil on relative fitness components via plasticity was a decrease in male fitness caused by reduced pollinator rewards (nectar production). Early snow melt and reduced precipitation are strong drivers of phenotypic plasticity, and both should be considered when predicting effects of climate change on plant traits in snow-dominated ecosystems.

Variation in floral volatiles across time, sexes, and populations of wind-pollinated Schiedea globosa.

PREMISE: Floral scent is a key aspect of plant reproduction, but its intraspecific variation at multiple scales is poorly understood. Sexual dimorphism and temporal regulation of scent can be shaped by evolution, and interpopulation variation may be a bridge to species differences. We tested whether intraspecific chemical diversity in a wind-pollinated species where selection from biotic pollination is absent is associated with genetic divergence across the Hawaiian archipelago. METHODS: Floral volatiles from females, males, and hermaphrodites of subdioecious Schiedea globosa grown in a common environment from 12 populations were sampled day and night and analyzed by gas chromatography-mass spectrometry. Variation among groups was analyzed by constrained ordination. We also examined the relationships of scent dissimilarity to geographic and genetic distance between populations. RESULTS: Flowers increased total emissions at night through higher emissions of several ketones, oximes, and phenylacetaldehyde. Females emitted less total scent per flower at night but more of some aliphatic compounds than males, and males emitted more ketones and aldoximes. Scent differed among populations during day and night. Divergence in scent produced at night increased with geographic distance within 70-100 km and increased with genetic distance for males during the day and night, but not for females. CONCLUSIONS: Schiedea globosa exhibits diel and sex-based variation in floral scent despite wind pollination and presumed loss of biotic pollination. In males, interpopulation scent differences are correlated with genetic differences, suggesting that scent evolved with dispersal within and across islands.

Evolution of sexual cooperation from sexual conflict.

In many species that form pair bonds, males display to their mate after pair formation. These displays elevate the female's investment into the brood. This is a form of cooperation because without the display, female investment is reduced to levels that are suboptimal for both sexes. The presence of such displays is paradoxical as in their absence the male should be able to invest extra resources directly into offspring, to the benefit of both sexes. We consider that the origin of these displays lies in the exploitation of preexisting perceptual biases which increase female investment beyond that which is optimal for her, initially resulting in a sexual conflict. We use a combined population genetic and quantitative genetic model to show how this conflict becomes resolved into sexual cooperation. A cooperative outcome is most likely when perceptual biases are under selection pressures in other contexts (e.g., detection of predators, prey, or conspecifics), but this is not required. Cooperation between pair members can regularly evolve even when this provides no net advantage to the pair and when the display itself reduces a male's contributions to raising the brood. The findings account for many interactions between the sexes that have been difficult to explain in the context of sexual selection.

Effect of surface pretreatment and artificial aging on the retention of lithium disilicate crowns cemented to zirconia implant abutments.

STATEMENT OF PROBLEM: Recent advancements in restorative dentistry have seen an increase in the use of ceramic restorations and zirconia implant abutments. However, how the pretreatment of a zirconia abutment and different artificial aging protocols affect the bond strength of a cemented, monolithic lithium disilicate crown is unclear. PURPOSE: The purpose of this in vitro study was to evaluate the effect of surface pretreatment on the retentive strength of milled lithium disilicate crowns bonded to custom zirconia implant abutments with different resin cements after thermocycling and long-term aging. MATERIAL AND METHODS: A total of 144 crowns (n=8) were milled and bonded to 144 abutments. In the experimental groups, 72 abutments were airborne-particle abraded with 50-µm aluminum oxide before bonding. All specimens were stored at 37 °C in 100% humidity for 24 hours. Forty-eight specimens were subjected to thermocycling, and another 48 were subjected to aging for 6 months. Retentive strength was measured by using a pull-off test with a universal testing machine. Retentive strength values were calculated and compared with 3-way analysis of variance and a Tukey-Kramer post hoc test (α=.05). RESULTS: In the 24-hour aging group, retention for all experimental groups was significantly higher (P<.05) than for the control group, except for Panavia 21 with Clearfil Ceramic Primer. In the thermocycling and long-term aging groups, all cements in the experimental group displayed significantly higher retention than the control. The airborne-particle abrasion of custom zirconia implant abutments with 50-µm aluminum oxide before bonding to lithium disilicate crowns significantly increased the bond strength of the Multilink Hybrid Abutment with Monobond Plus and RelyX Ultimate with Scotchbond Universal cements after 24-hour aging, but not of Panavia 21 with Clearfil Ceramic Primer. CONCLUSIONS: Airborne-particle abrasion significantly increased the bond strength of all 3 cements after thermocycling and long-term aging.

Instrumental and visual evaluation of the color adjustment potential of resin composites.

OBJECTIVE: To evaluate the instrumental and visual color adjustment potential (CAP-I, CAP-V), of resin composites. METHODS: Resin composites tested were Omnichroma, Filtek Supreme Ultra, TPH Spectra, Herculite Ultra, and Tetric EvoCeram. Two types of specimens, "dual" and "single" were evaluated. For dual specimens, class I preparations (4 mm-diameter, 2 mm-depth), were created in denture teeth and restored with test resin composites. For single specimens, replications of denture teeth were created using the test resin composites (n = 5) and compared with unrestored denture teeth. Reflectance values were obtained using a spectroradiometer. CAP-I and CAP-V were calculated. Fisher PLSD intervals for comparison of means were calculated at the 0.05 level of significance. RESULTS: CAP-I ranged between 0.02 and 0.67. The Fisher PLSD interval for comparisons between single/dual color differences was 0.09 (P < .0001, power 1.0) and for comparisons between materials and shades 0.18 and 0.33, respectively (P < .0001, power 1.0). CAP-V ranged between 0.16 and 0.60. The Fisher PLSD interval for comparisons between single/dual visual color differences was 0.05 (P < .0001, power 1.0) and 0.08 and 0.14 for comparisons between materials and shades, respectively (P < .0001, except Herculite Ultra-Tetric EvoCeram and certain shade pairs). CONCLUSIONS: Instrumental color differences and visual rating of single specimens were the greatest for Omnichroma, indicating the largest mismatch, while the instrumental and visual color differences of dual specimens were the smallest. Omnichroma had the most pronounced CAP-I followed by Tetric EvoCeram>TPH Spectra = Filtek Supreme Ultra >Herculite Ultra. Similarly, Omnichroma exhibited the most pronounced CAP-V followed by Tetric EvoCeram>TPH Spectra = Herculite Ultra = Filtek Supreme Ultra. CLINICAL SIGNIFICANCE: Resin composites with pronounced CAP blends with surrounding enamel and dentin, resulting in reduced color differences and therefore improving the esthetic appearance of the restoration, simplifying the shade matching and compensating for any color mismatch.

Adeno-associated Virus (AAV) Assembly-Activating Protein Is Not an Essential Requirement for Capsid Assembly of AAV Serotypes 4, 5, and 11.

Adeno-associated virus (AAV) vectors have made great progress in their use for gene therapy; however, fundamental aspects of AAV's capsid assembly remain poorly characterized. In this regard, the discovery of assembly-activating protein (AAP) sheds new light on this crucial part of AAV biology and vector production. Previous studies have shown that AAP is essential for assembly; however, how its mechanistic roles in assembly might differ among AAV serotypes remains uncharacterized. Here, we show that biological properties of AAPs and capsid assembly processes are surprisingly distinct among AAV serotypes 1 to 12. In the study, we investigated subcellular localizations and assembly-promoting functions of AAP1 to -12 (i.e., AAPs derived from AAV1 to -12, respectively) and examined the AAP dependence of capsid assembly processes of these 12 serotypes using combinatorial approaches that involved immunofluorescence and transmission electron microscopy, barcode-Seq (i. e., a high-throughput quantitative method using DNA barcodes and a next-generation sequencing technology), and quantitative dot blot assays. This study revealed that AAP1 to -12 are all localized in the nucleus with serotype-specific differential patterns of nucleolar association; AAPs and assembled capsids do not necessarily colocalize; AAPs are promiscuous in promoting capsid assembly of other serotypes, with the exception of AAP4, -5, -11, and -12; assembled AAV5, -8, and -9 capsids are excluded from the nucleolus, in contrast to the nucleolar enrichment of assembled AAV2 capsids; and, surprisingly, AAV4, -5, and -11 capsids are not dependent on AAP for assembly. These observations highlight the serotype-dependent heterogeneity of the capsid assembly process and challenge current notions about the role of AAP and the nucleolus in capsid assembly. IMPORTANCE: Assembly-activating protein (AAP) is a recently discovered adeno-associated virus (AAV) protein that promotes capsid assembly and provides new opportunities for research in assembly. Previous studies on AAV serotype 2 (AAV2) showed that assembly takes place in the nucleolus and is dependent on AAP and that capsids colocalize with AAP in the nucleolus during the assembly process. However, through the investigation of 12 different AAV serotypes (AAV1 to -12), we find that AAP is not an essential requirement for capsid assembly of AAV4, -5, and -11, and AAP, assembled capsids, and the nucleolus do not colocalize for all the serotypes. In addition, we find that there are both serotype-restricted and serotype-promiscuous AAPs in their assembly roles. These findings challenge widely held beliefs about the importance of the nucleolus and AAP in AAV assembly and show the heterogeneous nature of the assembly process within the AAV family.

Color adjustment potential of resin composites.

OBJECTIVES: The purpose of this study was to evaluate color adjustment potential (CAP) of resin composites. MATERIALS AND METHODS: Two shades of each of eight commercial resin composites and one control shade were evaluated. Visual (color competent observers, controlled conditions) and instrumental color evaluations (spectroradiometer, spectrophotometer) were performed. The data were analyzed by analysis of variance, Fisher's PLSD intervals for comparison of means, and Spearman's rank order correlation. RESULTS: Instrumental color adjustment potential (CAP-I) ranged from - 0.51 to 0.74, and corresponding Fisher's PLSD intervals were 0.1 and 0.05, respectively (p < 0.0001, power 1.0). Visual color adjustment potential (CAP-V) ranged from 0.10 to 0.78, and corresponding Fisher's PLSD intervals were 0.2 and 0.1, respectively (p < 0.0001, power 1.0). The greatest overall color shifting between test shades in isolation and the same shades surrounded by control shade were recorded for HRi ENA enamel, followed by Clearfil Majesty ES2. The highest visual CAP (blending) was recorded for Herculite Ultra, HRi ENA enamel, and Clearfil Majesty ES2. CONCLUSION: Within the limitation of the study, it was found that color adjustment potential (CAP) was composite and shade-dependent. Positive CAP was recorded both instrumentally and visually for majority of composites and shades. Overall, the measured color difference reduction associated with positive CAP was 31%, while the average visual CAP was 43%. CLINICAL RELEVANCE: Resin composites with pronounced color adjustment potential interact with surrounding dental restorations. Introduced CAP-V and CAP-I were indirect measurements of blending (optical illusion).

An enigmatic Hawaiian moth is a missing link in the adaptive radiation of Schiedea.

Shifts in pollination may drive adaptive diversification of reproductive systems within plant lineages. The monophyletic genus Schiedea is a Hawaiian lineage of 32 extant species, with spectacular diversity in reproductive systems. Biotic pollination is the presumed ancestral condition, but this key element of the life history and its role in shaping reproductive systems has remained undocumented. We observed floral visitors to two species of Schiedea and conducted field experiments to test pollinator effectiveness. We used choice tests to compare attraction of pollinators to species hypothesized to be biotically vs wind-pollinated. Pseudoschrankia brevipalpis (Erebidae), a recently described moth species known only from O'ahu, visited hermaphroditic Schiedea kaalae and S. hookeri and removed nectar from their unique tubular nectary extensions. Pseudoschrankia brevipalpis effectively pollinates S. kaalae; single visits to emasculated flowers resulted in pollen transfer. In choice tests, P. brevipalpis strongly preferred these hermaphroditic species over two subdioecious species capable of wind pollination. A shift from biotic to abiotic pollination is clearly implicated in the diversification of reproductive systems within Schiedea. Abundant pollination by a previously unknown native moth in experimental and restored populations suggests the potential for restoration to re-establish native plant-pollinator interactions critical for production of outcrossed individuals with high fitness.

Retentive strength of implant-supported CAD-CAM lithium disilicate crowns on zirconia custom abutments using 6 different cements.

STATEMENT OF PROBLEM: The optimal retention of implant-supported ceramic crowns on zirconia abutments is a goal of prosthodontic treatment. PURPOSE: The purpose of this in vitro study was to evaluate the retentive strength of implant-supported IPS e.max CAD-CAM (e.max) crowns bonded to custom zirconia implant abutments with different cements. MATERIAL AND METHODS: An optical scan of a zirconia custom abutment and a complete-coverage modified crown was designed using an intraoral E4D scanner. One hundred twenty lithium disilicate crowns (IPS e.max CAD) were cemented to 120 zirconia abutment replicas with 1 of 6 cements: Panavia 21 (P21), Multilink Hybrid Abutment (MHA), RelyX Unicem 2 (RXU), RelyX Luting Plus (RLP), Ketac Cem (KC), and Premier Implant (PI). The specimens were stored at 37°C in 100% humidity for 24 hours. Half of the specimens were thermocycled for 500 cycles. The retentive force was measured using a pull-out test with a universal testing machine. Mean retentive strengths (MRS) were calculated using 2-way ANOVA and the Tukey-Kramer test (α=.05). RESULTS: The MRS (MPa) after 24-hour storage were P21 (3.1), MHA (2.5), RXU (2.5), RLP (1.3), KC (0.9), and PI (0.5). The MRS after thermocycling were MHA (2.5), P21 (2.2), RLP (1.8), KC (1.4), RXU (1.1), and PI (0.3). P21 had the highest MRS after 24-hour storage (P<.001), but after thermocycling MHA had the highest MRS (P<.001). RXU showed a significant decrease in MRS after thermocycling (P<.05). Cement residue was mostly retained on the zirconia abutments for P21, while for the other cements' residue was retained on the lithium disilicate crowns. CONCLUSIONS: The cements tested presented a range of retentive strengths, providing the clinician with a choice of more or less retentive cements. MHA was the most retentive cement after thermocycling. Thermocycling significantly affected the retentive strengths of the P21 and RXU cements.

Natural selection on floral morphology can be influenced by climate.

Climate has the potential to influence evolution, but how it influences the strength or direction of natural selection is largely unknown. We quantified the strength of selection on four floral traits of the subalpine herb Ipomopsis sp. in 10 years that differed in precipitation, causing extreme temporal variation in the date of snowmelt in the Colorado Rocky Mountains. The chosen floral traits were under selection by hummingbird and hawkmoth pollinators, with hawkmoth abundance highly variable across years. Selection for flower length showed environmental sensitivity, with stronger selection in years with later snowmelt, as higher water resources can allow translation of pollination success into fitness based on seed production. Selection on corolla width also varied across years, favouring narrower corolla tubes in two unusual years with hawkmoths, and wider corollas in another late snowmelt year. Our results illustrate how changes in climate could alter natural selection even when the primary selective agent is not directly influenced.

Color stability of denture teeth and acrylic base resin subjected daily to various consumer cleansers.

OBJECTIVE: This study evaluated color stability of acrylic denture teeth and base resins after 48 weeks of commercial denture cleanser simulation. MATERIALS AND METHODS: Two brands of denture teeth (Trubyte Portrait IPN, TP; SR Vivodent DCL, SR) in shades A1, B1, and C1 and three acrylic base resins (Lucitone, LU; Paragon, PA; Valplast, VA) prepared to manufacturer's specifications were exposed 10 hours daily to four cleansers (Clorox Bleach, CB; Polident 3 minute, PO3; Efferdent, EF; and Kleenite, KL) and distilled water control, approximating consumer overnight use. Color measurements used the standard Commision International de l'Eclairage (International Commission on Illumination, CIE L*a*b*) color space (0, 4, 12, 24, 36, and 48 weeks.) Color differences (ΔE*) at 48 weeks were subjected to four-way analysis of variance (ANOVA). Mean values were compared with Fisher's (protected least significant difference) intervals (0.05 significance level). RESULTS: Mean color differences (ΔE*) demonstrated color changes in each material. ANOVA-indicated color changes in teeth were significantly affected by both cleansers and teeth brand (p < 0.05), but not shade. Color changes in base resins were significantly affected by cleansers (p < 0.05), but not brand alone. Overall, KL produced the least color change while CB and PO3 produced the most for all materials. CONCLUSIONS: After 48 weeks of daily simulation, TP teeth were more color stable than SR in all cleansers except EF (p < 0.0001). Base resin VA was less color stable than LU and PA. Cleanser KL resulted in the lowest color changes. CLINICAL SIGNIFICANCE: All tested materials yield clinically acceptable color changes (ΔE* < 3.5); all cleansing methods tested can be recommended, although Kleenite demonstrated the least change after 48 weeks.

Accelerated aging effects on color and translucency of flowable composites.

OBJECTIVES: To evaluate the effect of accelerated aging on color and translucency parameter of composites. MATERIALS AND METHODS: Filtek Supreme Ultra Flowable, shades A1, A3, White, Tetric EvoFlow, shades A1, A3, Bleach, and Filtek Z250 shades A1, A3, B1 were evaluated. Color and translucency were recorded using a spectrophotometer after 24-hour storage (baseline) and after accelerated aging. The data were analyzed by analysis of variance. RESULTS: Fisher's PLSD intervals for comparisons among products and shades were 0.4, 0.1, 0.3, and 0.4 for ΔL*, Δa*, Δb*, and ΔE*, respectively. The data showed significant ΔE* and ΔL* main effects for composite product and shades (p < 0.001) and their interactions (p = 0.008 and p = 0.03), respectively. Tetric EvoFlow displayed the smallest aging-dependent color shift followed by Filtek Z250 and Filtek Supreme Ultra Flowable. Accelerated aging did not significantly affected the composites for translucency (p > 0.05). CONCLUSION: The effects of aging on color of resin composites were composite and shade dependent. Translucency was not affected by accelerated aging. CLINICAL SIGNIFICANCE: The effects of aging on color of resin composites were composite and shade dependent.

Restoration of endodontically treated teeth with major hard tissue loss - influence of post surface design on pull-out bond strength of fiber-reinforced composite posts.

AIM: The aim was to evaluate the influence of post surface design and luting system on bond strength of quartz-fiber-reinforced composite posts (QFRCPs) luted to root canal dentin. MATERIALS AND METHODS: Single-rooted bovine teeth (n = 650) were randomly assigned (13 groups, n = 50), sectioned, endodontically treated, filled, and post space (length 8 mm) prepared. Custom-made plain-surfaced fiber posts (PSXRO) and (both RTD) macroretentive Macro-Lock Post Illusion X-RO (MLXRO) were inserted into the post spaces using six luting systems: Ketac Cem (KC), Fuji Plus (FP), RelyX Unicem, Multilink Primer_Multilink, Sealbond Ultima_CoreCem, and LuxaBond_LuxaCore Z. As control, a titanium post was cemented with KC. After water storage (24 h, 37°C), pull-out test was performed, followed by failure mode assessment. Bond strength was calculated in MPa and analyzed using anova, Dunnett-T3-test, and Student's t-test with Bonferroni correction. RESULTS: Post design and luting system significantly influenced the bond strength [MPa] (P < 0.05). Compared with the control 4.3 (1.5), all test groups exhibited higher bond strengths (P < 0.05), except for group PSXRO/KC 4.2 (1.0). The remaining bond strengths were PSXRO: FP 8.6 (1.5), RelyX Unicem 10.4 (3.4), Multilink Primer_Multilink 12.7 (3.0), SealBond Ultima_CoreCem 12.7 (3.0), LuxaBond_LuxaCore Z 15.7 (2.5), and MLXRO: KC 7.2 (2.2), FP 13.4 (2.5), RelyX Unicem 9.2 (2.9), Multilink Primer_Multilink 12.5 (4.5), SealBond Ultima_CoreCem 13.7 (4.6), LuxaBond_LuxaCore Z 20.6 (2.2). The bond strengths of MLXRO were higher than those of PSXRO when luted with KC, FP, and LuxaBond_LuxaCore Z (P < 0.05). CONCLUSION: The post surface design and luting system selection influenced the bond strength of conventionally and adhesively luted QFRCPs to bovine root canal dentin.

Pollinator and habitat-mediated selection as potential contributors to ecological speciation in two closely related species.

In ecological speciation, incipient species diverge due to natural selection that is ecologically based. In flowering plants, different pollinators could mediate that selection (pollinator-mediated divergent selection) or other features of the environment that differ between habitats of 2 species could do so (environment-mediated divergent selection). Although these mechanisms are well understood, they have received little rigorous testing, as few studies of divergent selection across sites of closely related species include both floral traits that influence pollination and vegetative traits that influence survival. This study employed common gardens in sites of the 2 parental species and a hybrid site, each containing advanced generation hybrids along with the parental species, to test these forms of ecological speciation in plants of the genus Ipomopsis. A total of 3 vegetative traits (specific leaf area, leaf trichomes, and photosynthetic water-use efficiency) and 5 floral traits (corolla length and width, anther insertion, petal color, and nectar production) were analyzed for impacts on fitness components (survival to flowering and seeds per flower, respectively). These traits exhibited strong clines across the elevational gradient in the hybrid zone, with narrower clines in theory reflecting stronger selection or higher genetic variance. Plants with long corollas and inserted anthers had higher seeds per flower at the Ipomopsis tenuituba site, whereas selection favored the reverse condition at the Ipomopsis aggregata site, a signature of divergent selection. In contrast, no divergent selection due to variation in survival was detected on any vegetative trait. Selection within the hybrid zone most closely resembled selection within the I. aggregata site. Across traits, the strength of divergent selection was not significantly correlated with width of the cline, which was better predicted by evolvability (standardized genetic variance). These results support the role of pollinator-mediated divergent selection in ecological speciation and illustrate the importance of genetic variance in determining divergence across hybrid zones.

A Novel Concept for Developing a Precise and Predictable Post and Core Complex Using the Injectable Resin Technique.

Post systems have long been used in the roots of teeth to retain dental restorations. The primary objectives of current post and core systems are to replace missing coronal tooth structure, retain the core, and provide sufficient retention and resistance form to the final restoration to restore original form and function. Many varieties of user-friendly post and core systems are available today for different endodontic, restorative, and esthetic requirements. The present article provides a history of post and core usage in dentistry, describes various systems and materials for this purpose, and discusses empirical data regarding fiber-reinforced post systems.