Application of high hydrostatic pressure on Pacific white shrimp ( Litopenaeus vannamei) pâté: Microbiological, physicochemical and consumer acceptance.

The locally elaborated shrimp pâté is highly susceptible to microbial spoilage and deterioration during storage due to its content in nutrients. These conditions limit its commercialization in a larger scale. High hydrostatic pressure is an alternative to heat treatments technology used to inactivate microorganisms. The aim of this project was to evaluate the effect of high hydrostatic pressure on quality parameters and microbiological stability of Pacific white shrimp ( Litopenaeus vannamei) pâté during storage. Shrimp pâté was pressurized to 400, 500 and 600 MPa for 90 and 180 s. Samples were analysed for physicochemical, microbiological and flavour profile up to 21 days of storage at 4 ℃. A hedonic test was made to evaluate the acceptance of pâté. No microorganisms were detected at 600 MPa for 180 s and a shelf life of 14 days was reached. No relevant changes in pH or colour of pressurized samples were detected; flavour profile did not show any changes after being pressurized or during storage. Shrimp pâté treated with 600 MPa for 180 s presented good sensory acceptance. High hydrostatic pressure treatments could improve microbiological quality of shrimp pâté without a sensible modification of the physicochemical and sensorial qualities of this product.

Effect of diets with different energy concentrations on growth performance, carcass characteristics and meat chemical composition of broiler chickens in dry tropics.

BACKGROUND: Diets with increasing levels of energy were fed for 42 days to 200, 1-day old male broiler chickens to evaluate growth performance, carcass characteristics and chemical composition of meat. The study was performed in the subtropical area of northeastern Mexico. Treatments diets (T) for starter and finisher phases had apparent metabolizable energy (AME; kcal/kg) of: 2960 and 3040 (T1); 3000 and 3080 (T2); 3040 and 3120 (T3); 3080 and 3160 (T4), respectively. Within each of the growing phases the four treatment diets were formulated to contain similar levels of crude protein, amino acids, and other nutrients. In a completely randomized design, birds were allocated to the four treatments with five replicates (floor pens) of 10 birds each. The trial was divided in two phases (starter and finisher) of 21 days each (42 days total). RESULTS: Weight gain was not influenced by energy level; however, feed conversion efficiency was improved in the diets with 3040 and 3120 kcal/kg AME (T3; P < 0.05). There was no influence of treatment on total carcass weight or carcass cuts (P > 0.05). Meat from breast muscle had similar crude protein percentages among treatments; ether extract was higher in T1 than T4 (P < 0.05). The percentages of water, ether extract, ash and crude protein in thigh meat were not significantly different (P > 0.05) among treatments. CONCLUSIONS: For this study carried out in a dry tropical area, the moderate increase in dietary energy concentration (diet with 3040 and 3120 kcal/kg AME, T3) enhanced feed conversion efficiency of broiler chickens.

Using high pressure processing (HPP) to pretreat sugarcane bagasse.

High pressure processing (HPP) technology was used to modify the structural composition of sugarcane bagasse. The effect of pressure (0, 150 and 250 MPa), time (5 and 10 min) and temperature (25 and 50 °C) as well as the addition of phosphoric acid, sulfuric acid and NaOH during the HPP treatment were assessed in terms of compositional analysis of the lignocellulosic fraction, structural changes and crystallinity of the bagasse. The effect of HPP pretreatment on the bagasse structure was also evaluated on the efficiency of the enzymatic hydrolysis of bagasse. Results showed that 68.62 and 45.84% of the hemicellulose fraction was degraded by pretreating at 250 MPa with sulfuric and phosphoric acids, respectively. The removal of lignin (54.10%) was higher with the HPP-NaOH treatment. The compacted lignocellulosic structure of the raw bagasse was modified by the HPP treatments and showed few cracks, tiny holes and some fragments flaked off from the surface. Structural changes were higher at 250 MPa and 50 °C. The X ray diffraction (XRD) patterns of the raw bagasse showed a major diffraction peak of the cellulose crystallographic 2θ planes ranging between 22 and 23°. The distribution of the crystalline structure of cellulose was affected by increasing the pressure level. The HPP treatment combined with NaOH 2% led to the higher glucose yield (25 g/L) compared to the combination of HPP with water and acids (>5 g/L). Results from this work suggest that HPP technology may be used to pretreat sugarcane bagasse.