Aflatoxin M1 Determination in Infant Formulae Distributed in Monterrey, Mexico.

The occurrence of aflatoxin M1 (AFM1) in infant formulae commercialized in the metropolitan area of Monterrey (Nuevo León, Mexico) was determined by using immunoaffinity column clean-up followed by HPLC determination with fluorimetric detection. For this, 55 infant formula powders were classified in two groups, starter (49 samples) and follow-on (6 samples) formulae. Eleven of the evaluated samples (20%) presented values above the permissible limit set by the European Union for infant formulae (25 ng/L), ranging from 40 to 450 ng/L. The estimated daily intake (EDI) for AFM1 was determined employing the average body weight (bw) of the groups of age in the ranges of 0-6 and 6-12 months, and 1-2 years. The results evidenced high intake values, ranging from 1.56 to 14 ng/kg bw/day, depending on the group. Finally, with the EDI value, the carcinogenic risk index was determined, presenting a high risk for all the evaluated groups. Based on these results, it is a necessary extra effort by the regulatory agencies to reduce the AFM1 presence in infant formulae consumed in Mexico.

Psychometric properties of the Spanish version of the short-form Child Perceptions Questionnaire for 11-14-year-olds for assessing oral health needs of children.

OBJECTIVE: To evaluate the psychometric properties of the Spanish version of the short-form of the Children Perceived Oral Health Questionnaire aimed at assessing child's oral health-related quality of life of children aged 11-14 years (CPQ11-14 ). A secondary objective was to explore its screening qualities for evaluating oral health needs and immediate referral for treatment. METHODS: This cross-sectional study was conducted during August-December of 2011 in Monterrey, Mexico (n = 303 schoolchildren). The CPQ11-14 consisted of 16 items and 2 key questions. Dental caries was diagnosed by visual-tactile exam, and malocclusion by WHO index. We estimated Cronbach's alpha and Spearman's correlations and compared rank scores with the Mann-Whitney test. As we did not have a preestablished cutoff point score, we identified one with the best combination of sensitivity/specificity values using receiver operating characteristic curves. RESULTS: Internal consistency was 0.85. Correlation was 0.40 (P < 0.001) between CPQ11-14 scores and self-perceived oral health rating and 0.51 (P < 0.001) between CPQ11-14 score and general well-being rating (concurrent validity). The mean score was 11.6 ± 8.6 for children in need of oral care and 8.1 ± 7.2 for those not in need of oral care (P < 0.01; discriminating validity). Sensitivity was 79.4% and specificity 48.7%; the positive and negative predictive values were 81.6% and 45.2%, respectively. CONCLUSIONS: The Spanish version short-form CPQ11-14 registered acceptable psychometric properties. We were able to identify a cutoff point score with acceptable sensitivity and positive predictive value but still needs future validation before generalizing its use.