RESUMEN
We report and solidly interpret the infrared spectrum of both pristine and oxidized carbynes embedded in a pure-carbon matrix. The spectra probe separately the effects of oxidation on sp- and on sp(2)-hybridized carbon, and provide information on the stability of the different structures in an oxidizing atmosphere. The final products are mostly short end-oxidized carbynes anchored with a double bond to sp(2) fragments, plus an oxidized sp(2) amorphous matrix. Our results have important implications for the realization of carbyne-based nano-electronics devices and highlight the active participation of carbynes in astrochemical reactions where they act as carbon source for the promotion of more complex organic species.
RESUMEN
The dynamics of excited states in α,ω-dinaphthylpolyyne, a class of linear sp-carbon chains, has been investigated by ultrafast transient absorption spectroscopy and DFT//TDDFT calculations. We show that the role of molecular conformers, in which end-capped naphthalene rings are planar or perpendicular to the polyyne plane, is fundamental for understanding both the steady state properties, such as UV-Vis absorption spectra and vibronic transitions, and the ultrafast transient absorption features. In particular, we observed in one of the conformers the ultrafast formation of a narrow photo-induced absorption band rising within 30 ps. This band can be assigned to an inter-system crossing event leading to the formation of triplet excited states.
RESUMEN
Mammalian cells respond to stress by accumulating or activating a set of highly conserved proteins known as heat-shock proteins (HSPs). Several of these proteins interfere negatively with apoptosis. We show that the small HSP known as Hsp27 inhibits cytochrome-c-mediated activation of caspases in the cytosol. Hsp27 does not interfere with granzyme-B-induced activation of caspases, nor with apoptosis-inducing factor-mediated, caspase-independent, nuclear changes. Hsp27 binds to cytochrome c released from the mitochondria to the cytosol and prevents cytochrome-c-mediated interaction of Apaf-1 with procaspase-9. Thus, Hsp27 interferes specifically with the mitochondrial pathway of caspase-dependent cell death.
Asunto(s)
Apoptosis , Grupo Citocromo c/metabolismo , Proteínas de Choque Térmico/metabolismo , Proteínas de Neoplasias/metabolismo , Factor Inductor de la Apoptosis , Caspasas/metabolismo , Citosol/metabolismo , Citosol/fisiología , Activación Enzimática , Flavoproteínas/metabolismo , Proteínas de Choque Térmico HSP27 , Humanos , Proteínas de la Membrana/metabolismo , Chaperonas Moleculares , Proteínas de Neoplasias/genética , Células U937RESUMEN
Heat-shock protein 70 (Hsp70) has been reported to block apoptosis by binding apoptosis protease activating factor-1 (Apaf-1), thereby preventing constitution of the apoptosome, the Apaf-1/cytochrome c/caspase-9 activation complex [1,2]. Here we show that overexpression of Hsp70 protects Apaf-1-/- cells against death induced by serum withdrawal, indicating that Apaf-1 is not the only target of the anti-apoptotic action of Hsp70. We investigated the effect of Hsp70 on apoptosis mediated by the caspase-independent death effector apoptosis inducing factor (AIF), which is a mitochondrial intermembrane flavoprotein [3,4]. In a cell-free system, Hsp70 prevented the AIF-induced chromatin condensation of purified nuclei. Hsp70 specifically interacted with AIF, as shown by ligand blots and co-immunoprecipitation. Cells overexpressing Hsp70 were protected against the apoptogenic effects of AIF targeted to the extramitochondrial compartment. In contrast, an anti-sense Hsp70 complementary DNA, which reduced the expression of endogenous Hsp70, increased sensitivity to the lethal effect of AIF. The ATP-binding domain of Hsp70 seemed to be dispensable for inhibiting cell death induced by serum withdrawal, AIF binding and AIF inhibition, although it was required for Apaf-1 binding. Together, our data indicate that Hsp70 can inhibit apoptosis by interfering with target proteins other than Apaf-1, one of which is AIF.
Asunto(s)
Apoptosis/fisiología , Fibroblastos/citología , Fibroblastos/fisiología , Flavoproteínas/metabolismo , Proteínas HSP70 de Choque Térmico/metabolismo , Proteínas de la Membrana/metabolismo , Proteínas/metabolismo , Animales , Factor Inductor de la Apoptosis , Factor Apoptótico 1 Activador de Proteasas , Núcleo Celular/ultraestructura , Supervivencia Celular , Células Cultivadas , Cromatina/fisiología , Cromatina/ultraestructura , Medio de Cultivo Libre de Suero , Flavoproteínas/genética , Proteínas de la Membrana/genética , Ratones , Proteínas/antagonistas & inhibidores , Proteínas/genética , Proteínas Recombinantes/metabolismo , TransfecciónRESUMEN
Apaf-1(-/-) or caspase-3(-/-) cells treated with a variety of apoptosis inducers manifest apoptosis-associated alterations including the translocation of apoptosis-inducing factor (AIF) from mitochondria to nuclei, large scale DNA fragmentation, and initial chromatin condensation (stage I). However, when compared with normal control cells, Apaf-1(-/-) or caspase-3(-/-) cells fail to exhibit oligonucleosomal chromatin digestion and a more advanced pattern of chromatin condensation (stage II). Microinjection of such cells with recombinant AIF only causes peripheral chromatin condensation (stage I), whereas microinjection with activated caspase-3 or its downstream target caspase-activated DNAse (CAD) causes a more pronounced type of chromatin condensation (stage II). Similarly, when added to purified HeLa nuclei, AIF causes stage I chromatin condensation and large-scale DNA fragmentation, whereas CAD induces stage II chromatin condensation and oligonucleosomal DNA degradation. Furthermore, in a cell-free system, concomitant neutralization of AIF and CAD is required to suppress the nuclear DNA loss caused by cytoplasmic extracts from apoptotic wild-type cells. In contrast, AIF depletion alone suffices to suppress the nuclear DNA loss contained in extracts from apoptotic Apaf-1(-/-) or caspase-3(-/-) cells. As a result, at least two redundant parallel pathways may lead to chromatin processing during apoptosis. One of these pathways involves Apaf-1 and caspases, as well as CAD, and leads to oligonucleosomal DNA fragmentation and advanced chromatin condensation. The other pathway, which is caspase-independent, involves AIF and leads to large-scale DNA fragmentation and peripheral chromatin condensation.
Asunto(s)
Apoptosis/fisiología , Caspasas/metabolismo , Núcleo Celular/metabolismo , Flavoproteínas/metabolismo , Proteínas de la Membrana/metabolismo , Mitocondrias/metabolismo , Proteínas/metabolismo , Animales , Antineoplásicos/farmacología , Factor Inductor de la Apoptosis , Factor Apoptótico 1 Activador de Proteasas , Arsenitos/farmacología , Caspasa 3 , Caspasas/genética , Células Cultivadas , Cisplatino/farmacología , Grupo Citocromo c/genética , Grupo Citocromo c/metabolismo , Fragmentación del ADN , Desoxirribonucleasas/genética , Desoxirribonucleasas/metabolismo , Etopósido/farmacología , Fibroblastos/metabolismo , Fibroblastos/ultraestructura , Flavoproteínas/genética , Colorantes Fluorescentes/metabolismo , Células HeLa , Humanos , Proteínas de la Membrana/genética , Ratones , Ratones Noqueados , Microinyecciones , Proteínas/genética , Proteínas Recombinantes/metabolismoRESUMEN
Viral protein R (Vpr) encoded by HIV-1 is a facultative inducer of apoptosis. When added to intact cells or purified mitochondria, micromolar and submicromolar doses of synthetic Vpr cause a rapid dissipation of the mitochondrial transmembrane potential (DeltaPsi(m)), as well as the mitochondrial release of apoptogenic proteins such as cytochrome c or apoptosis inducing factor. The same structural motifs relevant for cell killing are responsible for the mitochondriotoxic effects of Vpr. Both mitochondrial and cytotoxic Vpr effects are prevented by Bcl-2, an inhibitor of the permeability transition pore complex (PTPC). Coincubation of purified organelles revealed that nuclear apoptosis is only induced by Vpr when mitochondria are present yet can be abolished by PTPC inhibitors. Vpr favors the permeabilization of artificial membranes containing the purified PTPC or defined PTPC components such as the adenine nucleotide translocator (ANT) combined with Bax. Again, this effect is prevented by addition of recombinant Bcl-2. The Vpr COOH terminus binds purified ANT, as well as a molecular complex containing ANT and the voltage-dependent anion channel (VDAC), another PTPC component. Yeast strains lacking ANT or VDAC are less susceptible to Vpr-induced killing than control cells yet recover Vpr sensitivity when retransfected with yeast ANT or human VDAC. Hence, Vpr induces apoptosis via a direct effect on the mitochondrial PTPC.
Asunto(s)
Apoptosis , Productos del Gen vpr/fisiología , VIH-1/fisiología , Mitocondrias/fisiología , Sistema Libre de Células , Productos del Gen vpr/química , Humanos , Células Jurkat , Permeabilidad , Proteínas Proto-Oncogénicas c-bcl-2/fisiología , Productos del Gen vpr del Virus de la Inmunodeficiencia HumanaRESUMEN
The molecular mode of action of lonidamine, a therapeutic agent employed in cancer chemotherapy, has been elusive. Here we provide evidence that lonidamine (LND) acts on mitochondria to induce apoptosis. LND provokes a disruption of the mitochondrial transmembrane potential which precedes signs of nuclear apoptosis and cytolysis. The mitochondrial and cytocidal effects of LND are not prevented by inhibitors of caspases or of mRNA or protein synthesis. However, they are prevented by transfection-enforced overexpression of Bcl-2, an oncoprotein which inhibits apoptosis by stabilizing the mitochondrial membrane barrier function. Accordingly, the cell death-inducing effect of LND is amplified by simultaneous addition of PK11195, an isoquinoline ligand of the peripheral benzodiazepine receptor which antagonizes the cytoprotective effect of Bcl-2. When added to isolated nuclei, LND fails to provoke DNA degradation unless mitochondria are added simultaneously. In isolated mitochondria, LND causes the dissipation of the mitochondrial inner transmembrane potential and the release of apoptogenic factors capable of inducing nuclear apoptosis in vitro. Thus the mitochondrion is the subcellular target of LND. All effects of LND on isolated mitochondria are counteracted by cyclosporin A, an inhibitor of the mitochondrial PT pore. We therefore tested the effect of LND on the purified PT pore reconstituted into liposomes. LND permeabilizes liposomal membranes containing the PT pore. This effect is prevented by addition of recombinant Bcl-2 protein but not by a mutant Bcl-2 protein that has lost its apoptosis-inhibitory function. Altogether these data indicate that LND represents a novel type of anti-cancer agent which induces apoptosis via a direct effect on the mitochondrial PT pore.
Asunto(s)
Antineoplásicos/farmacología , Apoptosis , Indazoles/farmacología , Canales Iónicos/efectos de los fármacos , Mitocondrias/efectos de los fármacos , Proteínas Proto-Oncogénicas c-bcl-2/biosíntesis , Clorometilcetonas de Aminoácidos/farmacología , Sistema Libre de Células , Inhibidores de Cisteína Proteinasa/farmacología , Humanos , Canales Iónicos/metabolismo , Isoquinolinas/farmacología , Mitocondrias/metabolismo , Permeabilidad , Proteínas Proto-Oncogénicas c-bcl-2/genética , Proteínas Recombinantes/biosíntesis , Fracciones Subcelulares/metabolismo , Células U937RESUMEN
As shown here, mitochondria purified from different organs (liver, brain, kidney, spleen and heart) contain both pro-caspase-9 and the processed, mature form of caspase-9. Purified liver mitochondria release mature caspase-9 upon induction of permeability transition in vitro. This is accompanied by a discrete increase in the enzymatic cleavage of pro-caspase-9 substrates. We found that SHEP neuroblastoma cells constitutively contain pre-processed caspase-9 in their mitochondria, using a combination of subcellular fractionation and immunofluorescence with an antibody specific for the processed caspase. This is a cell type-specific phenomenon since HeLa cells mitochondria mainly contain pro-caspase-9 and comparatively little processed caspase-9. Upon introduction of apoptosis, mitochondrial pro-caspase-9 translocates to the cytosol and to the nucleus. This phenomenon is inhibited by transfection with Bcl-2. In synthesis, we report the unexpected finding that mitochondria can contain a pre-processed caspase isoform in non-apoptotic cells. Bcl-2-mediated regulation of mitochondrial membrane permeabilization may contribute to apoptosis control by preventing mitochondrial, pre-processed caspase-9 from interacting with its cytosolic activators.
Asunto(s)
Apoptosis/fisiología , Caspasas/fisiología , Mitocondrias/enzimología , Caspasa 9 , Caspasas/metabolismo , Núcleo Celular/enzimología , Citosol/enzimología , Inhibidores Enzimáticos/farmacología , Células HeLa , Humanos , Neuroblastoma , Transporte de Proteínas/efectos de los fármacos , Transporte de Proteínas/fisiología , Proteínas Proto-Oncogénicas c-bcl-2/biosíntesis , Proteínas Proto-Oncogénicas c-bcl-2/farmacología , Estaurosporina/farmacología , Células Tumorales CultivadasRESUMEN
Apoptosis-inducing factor (AIF) is encoded by one single gene located on the X chromosome. AIF is ubiquitously expressed, both in normal tissues and in a variety of cancer cell lines. The AIF precursor is synthesized in the cytosol and is imported into mitochondria. The mature AIF protein, a flavoprotein (prosthetic group: flavine adenine dinucleotide) with significant homology to plant ascorbate reductases and bacterial NADH oxidases, is normally confined to the mitochondrial intermembrane space. In a variety of different apoptosis-inducing conditions, AIF translocates through the outer mitochondrial membrane to the cytosol and to the nucleus. Ectopic (extra-mitochondrial) AIF induces nuclear chromatin condensation, as well as large scale ( approximately 50 kb) DNA fragmentation. Thus, similar to cytochrome c, AIF is a phylogenetically old, bifunctional protein with an electron acceptor/donor (oxidoreductase) function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion. The molecular mechanisms via which AIF induces apoptosis are discussed.
Asunto(s)
Apoptosis/fisiología , Flavoproteínas/metabolismo , Proteínas de la Membrana/metabolismo , Mitocondrias/metabolismo , Oxidorreductasas/metabolismo , Animales , Apoptosis/efectos de los fármacos , Factor Inductor de la Apoptosis , ADN Complementario/genética , Flavoproteínas/genética , Flavoproteínas/farmacología , Expresión Génica , Humanos , Proteínas de la Membrana/genética , Proteínas de la Membrana/farmacología , Oxidorreductasas/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Distribución Tisular , Cromosoma X/genéticaRESUMEN
Mitochondrial intermembrane proteins including cytochrome c are known to activate caspases. Accordingly, a disruption of the mitochondrial membrane barrier function with release of cytochrome into the cytosol has been shown to precede caspase activation in a number of different models of apoptosis. Here, we addressed the question of whether caspases themselves can affect mitochondrial membrane function. Recombinant caspases were added to purified mitochondria and were found to affect the permeability of both mitochondrial membranes. Thus, caspases cause a dissipation of the mitochondrial inner transmembrane potential. In addition, caspases cause intermembrane proteins including cytochrome c and AIF (apoptosis-inducing factor) to be released through the outer mitochondrial membrane. These observations suggest that caspases and mitochondria can engage in a circular self-amplification loop. An increase in mitochondrial membrane permeability would cause the release of caspase activators, and caspases, once activated, would in turn increase the mitochondrial membrane permeability. Such a self-amplifying system could accelerate the apoptotic process and/or coordinate the apoptotic response between different mitochondria within the same cell.
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Cisteína Endopeptidasas/metabolismo , Membranas Intracelulares/metabolismo , Mitocondrias Hepáticas/metabolismo , Clorometilcetonas de Aminoácidos , Animales , Apoptosis , Atractilósido/farmacología , Caspasa 1 , Cisteína Endopeptidasas/genética , Grupo Citocromo c/análisis , Células HeLa , Humanos , Potenciales de la Membrana , Ratones , Mitocondrias Hepáticas/fisiología , Consumo de Oxígeno , Permeabilidad/efectos de los fármacos , Proteínas Recombinantes de FusiónRESUMEN
We report the production and characterization of a form of amorphous carbon with s p-s p(2) hybridization (atomic fraction of sp hybridized species > or =20%) where the predominant sp bonding appears to be (=C=C=)(n) cumulene. Vibrational and electronic properties have been studied by in situ Raman spectroscopy and electrical conductivity measurements. Cumulenic chains are substantially stable in high vacuum conditions for temperatures lower than 250 K and they influence the electrical transport properties of the s p-s p(2) carbon through a self-doping mechanism by pinning the Fermi level closer to one of the mobility gap edges. Upon heating above 250 K the cumulenic species decay to form graphitic nanodomains embedded in the s p(2) amorphous matrix thus reducing the activation energy of the material. This is the first example of a pure carbon system where the s p hybridization influences bulk properties.
RESUMEN
From the standpoint of existential phenomenology, the contact with reality lies in the very phocus of theory, being closely related to another basic conception: that of being-in-the-world. In order to ratify those conceptions, the author reviews some concepts imported from Kurt Lewin's Field Theory, among which: a) vital psychological space, embedding the subject and in close interchange with him; b) intrapsychic regions, having, to a certain extent, autonomous functions, but being related to each other and integrated into the higher unity of the subject. As both systems are interdependent, any modification of the equilibrium of one of them reverberates into the other's, and changes the general conditions of both of them. Reviewing, at the same time, Minkowski's views on schizophrenia, the author sets forth the production of an inner world that becomes autonomous and possesses a degree of reality that overwhelms the true outer world. There is not only the splitting from reality, but the creation of a whole fantastic field, in which the individual participates with all his vital availability. Both views lead to a similar contention: that in some pathological states, the primal link man-real world, is replaced by a new inner correspondence, focused on the imaginary and having effects similar to those of the real world.
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Existencialismo , Humanos , Psicología del EsquizofrénicoRESUMEN
Taking into account Paul Ricoeur's theories, two possible hermeneutical positions in front of Oedipus' drama are considered. One is to view it as a fatalistic destiny, bred into the darkest incestuous trends any infant is fighting against, and leading to unavoidable stigmata of everlasting nature. The other is to conceive it as the response to present naked truth, resulting from deep reflexion in order to attain real consciousness of oneself. The former is the "backwards conception", having its own limitations, in its very being buried into the past. The latter, having its projections forward, centers on anguish and anxiety aroused by consciousness of oneself, seeked by the alert responsible mind, able to afford reality and truth with all their thorough implications. The author's existential position embraces the secon conception which had been already exposed by Sophocles in Oedipus in Colonna. The dialectics are set between seeing and knowing. Tiresias is blind, but he can see, because he can afford truth, and its consequence, guilt. Oedipus must fight to attain the possibility of knowing, and must go into the ordeal of losing his eyes, as the only way to reach true self realization, through facing truth and bearing anguish.
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Complejo de Edipo , Teoría Psicoanalítica , HumanosRESUMEN
This second study carried out on an extract of two grass pollens (Poa pratensis and Phleum pratense) demonstrated the presence of N-acetilglucosamine and sialic acid in the extract (RFEX). Moreover the extract has confirmed its characteristics biologic activity i.e. the inhibition of the phenomenon of immune hemolysis with a different technique too, even having the antibodies of the treated animals the complement fixing activity. No appreciable changes of the immunoelectrophoresis pattern in the serum of the immunized mice have been noticed.
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Antígenos/análisis , Extractos Vegetales/análisis , Poaceae/análisis , Polen/análisis , Acetilglucosamina/análisis , Animales , Anticuerpos/análisis , Pruebas de Fijación del Complemento , Hemólisis , Ratones , Extractos Vegetales/inmunología , Poaceae/inmunología , Polen/inmunología , Ácidos Siálicos/análisisRESUMEN
The highly conserved heat shock proteins (HSPs) accumulate in cells exposed to heat and a variety of other stressful stimuli. HSPs, which function mainly as molecular chaperones, allow cells to adapt to gradual changes in their environment and to survive in otherwise lethal conditions. The events of cell stress and cell death are linked and HSPs induced in response to stress appear to function at key regulatory points in the control of apoptosis. HSPs include antiapoptotic and proapoptotic proteins that interact with a variety of cellular proteins. Their expression level can determine the fate of the cell in response to a death stimulus, and apoptosis-inhibitory HSPs, in particular HSP27 and HSP70, may participate in carcinogenesis. This review summarizes apoptosis-regulatory function of HSPs.
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Apoptosis , Proteínas de Choque Térmico/fisiología , Animales , Chaperonina 60/fisiología , Proteínas HSP70 de Choque Térmico/fisiología , Proteínas HSP90 de Choque Térmico/fisiología , Modelos Biológicos , Chaperonas Moleculares/fisiología , Neoplasias/metabolismo , Neoplasias/patología , Estrés Fisiológico/metabolismoRESUMEN
Proteasomes and mitochondrial membrane changes are involved in thymocyte apoptosis. The hierarchical relationship between protease activation and mitochondrial alterations has been elusive. Here we show that inhibition of proteasomes by two specific agents, lactacystin or MG132, prevents all manifestations of thymocyte apoptosis induced by the glucocorticoid receptor agonist dexamethasone or by the topoisomerase II inhibitor etoposide. Lactacystin and MG132 prevent the early disruption of the mitochondrial transmembrane potential (delta psi(m)), which precedes caspase activation, exposure of phosphatidylserine, and nuclear DNA fragmentation. In contrast, stabilization of the delta psi(m) using the permeability transition pore inhibitor bongkrekic acid or inhibition of caspases by N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone does not prevent the activation of proteasomes, as determined with the fluorogenic substrate N-succinyl-L-leucyl-L-leucyl-L-valyl-L-tyrosine-7-amido-4-methylcoumarin . Thus, proteasome activation occurs upstream from mitochondrial changes and caspase activation. Whereas the proteasome-specific agents lactacystin and MG132 truly maintain thymocyte viability, a number of protease inhibitors that inhibit nuclear DNA fragmentation (acetyl-Asp-Glu-Val-Asp-fluoromethylketone; N-Boc-Asp(OMe)-fluoromethylketone; N-tosyl-L-Phe-chloromethylketone) do not prevent the cytolysis induced by DEX or etoposide. These latter agents fail to interfere with the preapoptotic delta psi(m) disruption. Altogether, our data indicate that different proteases may be involved in the pre- or postmitochondrial phase of apoptosis. Only those protease inhibitors that interrupt the apoptotic process at the premitochondrial stage can actually preserve cell viability.
Asunto(s)
Apoptosis/inmunología , Cisteína Endopeptidasas/metabolismo , Mitocondrias/fisiología , Complejos Multienzimáticos/metabolismo , Linfocitos T/enzimología , Timo/enzimología , Animales , Apoptosis/efectos de los fármacos , Cisteína Endopeptidasas/efectos de los fármacos , Inhibidores de Cisteína Proteinasa/farmacología , Dexametasona/farmacología , Activación Enzimática/inmunología , Inhibidores Enzimáticos/farmacología , Etopósido/farmacología , Femenino , Membranas Intracelulares/metabolismo , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/inmunología , Ratones , Ratones Endogámicos BALB C , Complejos Multienzimáticos/efectos de los fármacos , Complejo de la Endopetidasa Proteasomal , Linfocitos T/citología , Timo/citologíaRESUMEN
Lipid and glycolipid diffusible mediators are involved in the intracellular progression and amplification of apoptotic signals. GD3 ganglioside is rapidly synthesized from accumulated ceramide after the clustering of death-inducing receptors and triggers apoptosis. Here we show that GD3 induces dissipation of DeltaPsim and swelling of isolated mitochondria, which results in the mitochondrial release of cytochrome c, apoptosis inducing factor, and caspase 9. Soluble factors released from GD3-treated mitochondria are sufficient to trigger DNA fragmentation in isolated nuclei. All these effects can be blocked by cyclosporin A, suggesting that GD3 is acting at the level of the permeability transition pore complex. We found that endogenous GD3 accumulates within mitochondria of cells undergoing apoptosis after ceramide exposure. Accordingly, suppression of GD3 synthase (ST8) expression in intact cells substantially prevents ceramide-induced DeltaPsim dissipation, indicating that endogenously synthesized GD3 induces mitochondrial changes in vivo. Finally, enforced expression of bcl-2 significantly prevents GD3-induced mitochondrial changes, caspase 9 activation, and apoptosis. These results show that mitochondria are a key destination for apoptogenic GD3 ganglioside along the lipid pathway to programmed cell death and indicate that relevant GD3 targets are under bcl-2 control.
Asunto(s)
Apoptosis , Gangliósidos/farmacología , Potenciales de la Membrana/efectos de los fármacos , Mitocondrias Hepáticas/efectos de los fármacos , Proteínas Proto-Oncogénicas c-bcl-2/biosíntesis , Animales , Caspasa 9 , Caspasas/metabolismo , Ciclosporina/farmacología , Activación Enzimática , Ratas , Sialiltransferasas/metabolismo , Fracciones Subcelulares/efectos de los fármacosRESUMEN
The fatty acid palmitate can induce apoptosis. Here we show that the palmitate-induced dissipation of the mitochondrial transmembrane potential (Delta Psi m), which precedes nuclear apoptosis, is not prevented by inhibitors of mRNA synthesis, protein synthesis, caspases, or pro-apoptotic ceramide signaling. However, the mitochondrial and nuclear effects of palmitate are inhibited by overexpression of anti-apoptotic proto-oncogene product Bcl-2 and exacerbated by 2-bromo-palmitate as well as by carnitine. The cytoprotective actions of Bcl-2, respectively, is not antagonized by etomoxir, an inhibitor of carnitine palmitoyl transferase 1 (CPT1), suggesting that the recently described physical interaction between CPT1 and Bcl-2 is irrelevant to Bcl-2-mediated inhibition of palmitate-induce apoptosis. When added to purified mitochondria, palmitate causes the release of soluble factors capable of stimulating the apoptosis of isolated nuclei in a cell-free system. Mitochondria purified from Bcl-2 over-expressing cells are protected against the palmitate-stimulated release of such factors. These data suggest that palmitate causes apoptosis via a direct effect on mitochondria.