Analysis of settled dust with X-ray Fluorescence for exposure assessment of metals in the province of Brescia, Italy.

Ferroalloy industries have been active for more than a century in the province of Brescia, Northern Italy. Air emission and water discharge have contaminated the environment in the surroundings of four plants with several metals including manganese. The presence of manganese in this region is especially interesting, because of the observed relationship between manganese exposure and Parkinsonism in a previous epidemiological survey. The aim of this study was represented by an initial screening of metal exposure in this area, using a geographic information system. X-ray fluorescence (XRF) was applied to identify heavy metals in deposited dust samples, collected in representative residential households throughout the province. The results were interpreted through a systematic mapping of all municipal districts of the Brescia province. A more frequent distribution of manganese and other metals was observed in the municipalities where the plants were located and differences in the geochemical and anthropogenic origin of metals were discussed, according to the point sources.

High prevalence of Parkinsonian disorders associated to manganese exposure in the vicinities of ferroalloy industries.

OBJECTIVE: To assess the prevalence of Parkinsonian disturbances in relationship to environmental exposure to manganese due to ferroalloy industries in the province of Brescia, Northern Italy. METHODS: Manganese concentrations were measured in settled dust collected in each of the 206 municipalities. Parkinsonian patients were identified using two sources: (1) clinical registers from local hospitals, specialized neurologists, and exemption from prescription payment; (2) L-Dopa prescriptions. Standardized prevalence rates and raw and full Bayesian-smoothed standardized morbidity ratios (SMRs) were calculated for the entire province and for each municipality. RESULTS: Manganese concentrations in settled dust were significantly higher in the surroundings and downwind from the industrial plants. A total number of 2,677 Parkinsonian cases were identified among 903,997 residents (crude prevalence, 296/100,000; 95% CI: 284.80-307.20; standardized prevalence, 407/100,000; 95% CI: 393.87-420.12). Significantly higher SMRs (Kruskal-Wallis chi(2) 1 df = 17.55, P < 0.001) were observed in 37 municipalities in the vicinities of ferromanganese plants (324 cases among 77,708 residents; standardized prevalence 492/100,000; 95% CI: 442.80-541.20), compared to the other 169 municipalities of the province (2,353 cases among 826,289 residents, standardized prevalence 321/100,000; 95% CI 308.80-333.20). Row and Bayesian SMRs were associated with the concentrations of manganese in settled dust. CONCLUSION: Study results suggest that environmental exposure to manganese is associated with an increased prevalence of Parkinsonian disturbances. Since the highest prevalence rates were observed in a closed community of the pre-Alps where the industries are located, further research should address a possible interactive role of genetic factors.

Soil contamination detected using bacterial and plant mutagenicity tests and chemical analyses.

Soil contaminants are common in industrialized countries, causing widespread contamination directly of soil and indirectly of ground water and food. Among these pollutants particular attention should be paid to soil mutagens and carcinogens due to their potentially hazardous effects on animal populations and human health. The aim of this research was to evaluate the genotoxicity of contaminated soils by means of an integrated chemical/biological approach, using a short-term bacterial mutagenicity test (Ames test), a plant genotoxicity test (Tradescantia/micronucleus test), and chemical analyses. Soil samples were collected in a highly industrialized area in the Lombardy region, in Northern Italy. Soil samples were extracted with water or with organic solvents. Water extracts of soil samples were tested using the Tradescantia genotoxicity test and organic solvent extracts were analyzed for their polycyclic aromatic hydrocarbon (PAH) concentrations and for their mutagenicity with the Ames test. Heavy metal concentrations were also studied. Some soil samples showed mutagenic activity with the Ames test and clastogenicity with the Tradescantia/micronucleus test. The same soils showed high concentrations of genotoxic PAH and heavy metals.