Effect of Staining, Glazing and Polishing on the Survival Probability of Monolithic Zirconia Crowns.

OBJECTIVE: The purpose of this in vitro study was to evaluate the effect of staining, glazing, and polishing on the survival probability of monolithic crowns manufactured with preshaded stabilized zirconia with 5 mol% of yttrium oxide (5Y-TZP). MATERIALS AND METHODS: Monolithic crowns in the shape of an upper canine (1.5 mm of thickness) were manufactured by CAD/CAM, adhesively cemented on metallic foundation, and divided into 6 groups (n = 21): C (control), S (staining), G (glazing), P (polishing), SG (staining and glazing), and SP (staining and polishing). The survival probability was determined by step-stress accelerated life testing with a load applied to the palatine concavity of the crown. First, the specimens were subjected to a single-load to fracture test (SLF) and next to the fatigue test (5 Hz, thermocycling immersed in water varying 5-55°C), including the light (n = 9), moderate (n = 6), and aggressive (n = 3) loading profiles (load ranged between 20% and 60% of SLF). The survival probability was calculated considering the cycles for failure (CFF) and fatigue failure load (FFL) and illustrated using a Kaplan-Meier graph. The comparison among groups was performed using a Log-Rank test (α = 0.05). RESULTS: The mean value of SLF was 586.7 N. There was no difference among groups in survival probability, considering CFF and FFL. CONCLUSION: Staining, glazing, and polishing can be performed safely without damaging the mechanical behavior of 5Y-TZP monolithic crowns. CLINICAL SIGNIFICANCE: Staining is used to characterize and improve the esthetic of zirconia monolithic crowns. It can be used to reproduce the color gradient in the cervical region of the crown and pigmented grooves. This study showed that staining, glazing, and polishing did not affect the survival probability and the use of finishing procedures (glazing or polishing) after staining did not improve the survival probability of zirconia monolithic crowns.

Evaluation of Vertical Misfit and Torque Loss of Different Abutments for Tri-Channel Type Internal Connection Dental Implants After Mechanical Cycling.

The aim of this study was to evaluate the mechanical behavior of UCLA and Mini-conical abutments for implants with Tri-channel connections regarding torque loss and vertical misfit. Twenty 3-element metal-ceramic fixed partial dentures (FPD) supported by 2 implants were manufactured and divided into 2 groups (n = 10): UCLA (group 1) and Mini-conical Abutments (group 2). The evaluation of torque loss was carried out before and after mechanical cycling, while the vertical fit was evaluated throughout the different stages of manufacturing the prostheses, as well pre- and postcycling (300,000 cycles, 30 N). Statistical analyses of torque loss and vertical misfit were performed using the linear mixed effects model. Both groups showed torque loss after mechanical cycling (P < .05); however, there was no significant percentage differences between them (P = .795). Before cycling, the groups showed a significant difference in terms of vertical misfit values (P < .05); however, this difference was no long observed after cycling (P = .894). Both groups showed torque loss after the cycling test, with no significant difference (P > .05). There was no significant difference in vertical misfit after mechanical cycling; however, in group 1 (UCLA) there was accommodation of the implant-UCLA abutment interface, while group 2 (Mini-conical abutment) did not show changes in the interface with the implant after the test. Both groups behaved similarly regarding the torque loss of the prosthesis retention screws pre- and postmechanical cycling, with greater loss after the test.

Mechanical Properties, Wear Resistance, and Reliability of Two CAD-CAM Resin Matrix Ceramics.

Background and Objectives: There are limited data regarding the behavior of resin matrix ceramics for current CAD-CAM materials. Further studies may be beneficial and can help clinicians planning to use these materials during prosthodontic rehabilitation. The aim of this study was to evaluate and compare the flexural strength and strain distributions, filler content, wear, and reliability of two resin matrix ceramic CAD-CAM materials. Materials and Methods: Two resin matrix ceramics, Ambarino High-Class (AH) and Vita Enamic (VE), were tested for flexural strength (n = 24), wear (n = 10), and reliability (n = 18). Thermogravimetric analysis was used to determine the percentage of filler by weight, and digital image correlation (DIC) was used for strain analysis in flexural strength test. Reliability of each resin matrix ceramic was compared after accelerated lifetime testing of crowns using a two-parameter Weibull distribution. Data of flexural strength, wear, and thermogravimetry were analyzed by independent t-tests with significance level at 5%. Results: The results of DIC analysis were analyzed by a qualitative comparison between the images obtained. The materials tested showed different flexural strength (p < 0.05) and strain distributions. The filler content was the same as informed by manufacturers. No difference was observed in the wear or reliability analysis (p > 0.05). The flexural strength of material AH was superior to VE, and the strain distribution was compatible with this finding. Conclusions: The two resin matrix ceramics tested showed similar behavior in wear and reliability analysis. Both can provide safe use for dental crowns.

Influence of Torque on Platform Deformity of the Tri-Channel Implant: Two- and Three-Dimensional Analysis Using Micro-Computed Tomography.

Background and Objectives: The insertion of the dental implant in the bone is an essential step in prosthetic rehabilitation. The insertion torque has the potential to distort the prosthetic platform, which can cause future biomechanical problems with the continuous action of occlusal forces. The aim of this study is to evaluate different insertion torques in the deformation of tri-channel platform connections through two- and three-dimensional measurements with micro-CT. Materials and Methods: A total of 164 implants were divided into groups (platform diameter and type): 3.5, 3.75, and 4.3 mm NP (narrow platform), and 4.3 mm RP (regular platform). Each implant-platform group was then divided into four subgroups (n = 10) with different torques: T45 (45 Ncm), T80 (80 Ncm), T120 (120 Ncm), and T150 (150 Ncm). The implant-abutment-screw assemblies were scanned and the images obtained were analyzed. Results: A significant difference was observed for the linear and volume measures between the different platforms (p < 0.01) and the different implant insertion torques (p < 0.01). Qualitative analysis suggested a higher deformation resistance for the 3.75 NP compared to the 3.5 NP, and RP was more resistant compared to the NP. Conclusions: The 0.25 mm increment in the implant platform did not increase the resistance to the applied insertion torques; the 4.3 mm implant was significantly stronger compared to the 3.5 mm implant; and the proposed micro-CT analysis was considered valid for both the 2D and 3D analyses of micro-gaps, qualitatively and quantitatively.

A completely digital workflow for an interim implant-supported crown: A clinical report.

This article describes a completely digital workflow for the diagnostic phase, surgical and prosthetic planning, extraction, immediate single implant placement by guided surgery, and interim implant-supported crown. From a virtual impression, zirconia abutments and a polymethylmethacrylate (PMMA) interim crown was planned in a computer-aided design (CAD) software program. This workflow shortened the time required for chairside placement of an interim restoration with enhanced function and esthetics while restoring an anterior mandibular tooth lost after trauma.

Influence of Different Implants on the Biomechanical Behavior of a Tooth-Implant Fixed Partial Dentures: A Three-Dimensional Finite Element Analysis.

This study analyzed the biomechanical behavior of rigid and nonrigid tooth-implant supported fixed partial dentures. Different implants were used to observe the load distribution over teeth, implants, and adjacent bone using three-dimensional finite element analysis. A simulation of tooth loss of the first and second right molars was created with an implant placed in the second right molar and a prepared tooth with simulated periodontal ligament (PDL) in the second right premolar. Configurations of two types of implants and their respective abutments-external hexagon (EX) and Morse taper (MT)-were transformed into a 3D format. Metal-ceramic fixed partial dentures were constructed with rigid and nonrigid connections. Mesh generation and data processing were performed on the 3D finite element analysis (FEA) results. Static loading of 50 N (premolar) and 100 N (implant) were applied. When an EX implant was used, with a rigid or nonrigid connection, there was intrusion of the tooth in the distal direction with flexion of the periodontal ligament. Tooth intrusion did not occur when the MT implant was used independent of a rigid or nonrigid connection. The rigid or nonrigid connection resulted in a higher incidence of compressive forces at the cortical bone as well as stress in the abutment/pontic area, regardless of whether EX or MT implants were used. MT implants have a superior biomechanical performance in tooth-implant supported fixed partial dentures. This prevents intrusion of the tooth independent of the connection. Both types of implants studied caused a greater tendency of compressive forces at the crestal area.

Genome analysis and clinical implications of the bacterial communities in early biofilm formation on dental implants restored with titanium or zirconia abutments.

This cross-sectional study aimed to identify and quantify up to 42 target species colonizing the early biofilm of dental implants restored with titanium or zirconia abutments. A total of 720 samples from 20 healthy individuals were investigated. Biofilm samples were collected from the peri-implant sulci, inner parts of implants, abutment surfaces and prosthetic crowns over a functioning period of 30 days. Checkerboard DNA-DNA hybridization was used for microbial detection and quantitation. Clinical characteristics (probing depth, bleeding on probing, clinical attachment level and marginal bone loss) were also investigated during the monitoring period. Genome counts were low at the implant loading time point for both the abutment materials, and increased over time. Both the titanium and the zirconia groups presented similar microbial counts and diversity over time, and the microbiota was very similar to that colonizing the remaining teeth. Clinical findings were consistent with a healthy condition with no significant difference regarding marginal bone loss between the two materials.

Oral bacterial colonization on dental implants restored with titanium or zirconia abutments: 6-month follow-up.

OBJECTIVE: This investigation aimed to characterize in a 6-month follow-up the microbial profile of implants restored with either titanium or zirconia abutments at the genus or higher taxonomic levels. METHODS: Twenty healthy individuals indicative for implant-retained single restorations were investigated. Half of participants were restored with titanium and half with zirconia abutments. Biofilm was collected from the implant-related sites after 1, 3, and 6 months of loading. The 16S rDNA genes were amplified and sequenced with Roche/454 platform. RESULTS: A total of 596 species were identified in 360 samples and grouped in 18 phyla and 104 genera. Titanium- or zirconia-related sites as well as teeth showed similar total numbers of operational taxonomic units (OTUs) colonizing surfaces over time. Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, and Actinobacteria were the most prevalent phyla with significant differences between different surfaces and time point. Unclassified genera were found in lower levels (1.71% up to 9.57%) on titanium and zirconia samples when compared with teeth, with no significant differences. CONCLUSION: Titanium- and zirconia-related surfaces are promptly colonized by a bacterial community similar to those found in the remaining adjacent teeth. Results suggest a selective adhesion of different bacterial genotypes for either titanium or zirconia surfaces. Data also indicate a significant interaction between the relative effects taxa, time point, and sampling site. CLINICAL RELEVANCE: The present study disclosed a wider spectrum of microorganisms colonizing either titanium- or zirconia-related microbiomes in very early stage of implant colonization, revealing differences and suggesting a probably specific mechanism for selective bacterial adhesion.

Strain transfer behavior of different planning options for mandibular single-molar replacement.

STATEMENT OF PROBLEM: The loss of the first molar and second premolar could lead to mesial movement of the second molar, thus limiting the restoration space for the 2 missing teeth. Placement of a larger first molar is a common choice, but the best implant number and position option remain controversial. PURPOSE: The purpose of this in vitro study was to test different planning options for replacing the mandibular first molar. MATERIAL AND METHODS: Two polyoxymethylene models simulated first molar edentulous spaces of 11 mm (conventional size first molar: control group) and 14 mm (enlarged first molar: all remaining groups other than control). Models included acrylic resin replicas of a first and second premolar, a second molar, and the first molar edentulous space. The following groups were established: control (CO), ø3.5-mm center implant; center implant (CI), ø3.5 mm; mesial implant (MI), ø3.5 mm; distal implant (DI), ø3.5 mm; center implant (WI), ø5.0; 2 implants (2I), 2 ø3.5-mm implants. Three Co-Cr molar crowns were fabricated for each group by using a computer-aided design and computer-aided manufacturing (CAD-CAM) technique. Model surface strains under a 250-N first molar load were calculated by 3-dimensional digital image correlation. Three regions of interest below the first molar were selected for comparison among groups. A test for unequal variances and a follow-up Welch ANOVA were used for statistical analysis (α=.05). RESULTS: The highest strains were found when the first molar was restored by using a 5.0-mm-wide implant (P<.05). Region of interest 1 showed that two 3.5-mm implants replacing the lost molar showed strain distribution similar to that of only one 3.5-mm implant (P>.05). Mesial and distal placement of the implant showed more neutral strain results than other restoration options (P<.05). CONCLUSIONS: Two small-diameter implants in an increased edentulous space show more optimized surface strain behavior than a single wide-diameter implant. However, a single 3.5-mm implant also showed reduced strains in the restoration of the same edentulous space.

Abutments with reduced diameter for both cement and screw retentions: analysis of failure modes and misfit of abutment-crown-connections after cyclic loading.

OBJECTIVE: The aim of this study was to analyze failure modes and misfit of abutments with reduced diameter for both cement and screw retentions after cyclic loading. MATERIAL AND METHODS: Forty morse-taper abutment/implant sets of titanium were divided into four groups (N = 10): G4.8S-4.8 abutment with screw-retained crown; G4.8C-4.8 abutment with cemented crown; G3.8S-3.8 abutment with screw-retained crown; and G3.8C-3.8 abutment with cemented crown. Copings were waxed on castable cylinders and cast by oxygen gas flame and injected by centrifugation. After, esthetic veneering ceramic was pressed on these copings for obtaining metalloceramic crowns of upper canine. Cemented crowns were cemented on abutments with provisional cement (Temp Bond NE), and screw-retained crowns were tightened to their abutments with torque recommended by manufacturer (10 N cm). The misfit was measured using a stereomicroscope in a 10× magnification before and after cyclic loading (300,000 cycles). Tests were visually monitored, and failures (decementation, screw loosening and fractures) were registered. Misfit was analyzed by mixed linear model while failure modes by chi-square test (α = 0.05). RESULTS: Cyclic loading affected misfit of 3.8C (P ≤ 0.0001), 3.8S (P = 0.0055) and 4.8C (P = 0.0318), but not of 4.8S (P = 0.1243). No differences were noted between 3.8S with 4.8S before (P = 0.1550) and after (P = 0.9861) cyclic loading, but 3.8C was different from 4.8C only after (P = 0.0015) loading. Comparing different types of retentions at the same diameter abutment, significant difference was noted before and after cyclic loading for 3.8 and 4.8 abutments. Analyzing failure modes, retrievable failures were present at 3.8S and 3.8C groups, while irretrievable were only present at 3.8S. CONCLUSIONS: The cyclic loading decreased misfit of cemented and screw-retained crowns on reduced diameter abutments, and misfit of cemented crowns is greater than screw-retained ones. Abutments of reduced diameter failed more than conventional.

Microleakage at Different Implant-Abutment Connections Under Unloaded and Loaded Conditions.

PURPOSE: This study evaluated the microleakage at different implant-abutment (I-A) connections under unloaded and loaded conditions. MATERIALS AND METHODS: Forty implants, specially designed with an opening at the apex, were grouped according to the I-A and screw device: external hexagon implants with titanium (EH) or EH diamond-like carbon screws fixing the abutment; internal hexagon implants with titanium screws (IH); and Morse taper implants with solid (MT) or MT passing screws (MTps) abutments. The implants were fixed in a 2-compartment device, and toluidine blue solution (1.0 mg/mL) was placed at the I-A interface (upper compartment). The lower compartment was filled with purified water. Four implants of each group were loaded (50 N, 1.2 Hz) and after 50,000, 100,000, 200,000, and 300,000 cycles, aliquots were collected from the lower compartment for absorbance reading. Data were analyzed using analysis of variance for repeated measurements and Tukey (α = 0.05). RESULTS: Although microleakage increased over time in most of the groups, MTps group showed lower values when loaded (P < 0.05). The DLC on the EH screw did not prevent microleakage (P < 0.05). CONCLUSION: It can be concluded that MT connection is more effective to prevent microleakage.

Influence of Cyclic Fatigue in Water on Screw Torque Loss of Long-Span One-Piece Implant-Supported Zirconia Frameworks.

PURPOSE: It is still unclear whether four, six, or more implants should be used when restoring fully edentulous maxillae. This research evaluated the in vitro screw torque loss of zirconia frameworks supported by six implants and cantilevered zirconia frameworks supported by four implants. MATERIALS AND METHODS: Computer aided design/computer-assisted machining was used to fabricate 10 one-piece frameworks. Standardized pressable porcelain crowns were fabricated and luted to the frameworks. Specimens were divided into two groups (n = 5): AO4, cantilevered 12-unit full-arch fixed dental prosthesis supported by four implants; AO6, 14-unit supported by six implants. An opposing mandibular dental arch was fabricated with bis-acrylic composite resin. Specimens were submitted to 200 N underwater cyclic load at 2-Hz frequency for 1 × 106 cycles in a controlled 37°C temperature. A digital torque gauge assessed the initial and postload screw removal torque. Linear mixed-effects model was used for statistical analysis (α = 0.05). RESULTS: Significant screw torque loss was found for AO6 after cyclic loading (before: 36.20%/after: 52.82%; p < 0.05). Group AO6 (36.20%) presented lower preload loss before the cyclic loadings compared with AO4 (60.10%) (p < 0.05). CONCLUSIONS: Cyclic loading and lower implant-to-replaced-units ratio do not seem to compromise screw stability compared with higher implant-to-replaced-units ratio; however, a steep drop in preload was found before cyclic loading for both groups.

Stress Over Implants of One-Piece Cast Frameworks Made With Different Materials.

This study aims to compare stress transmitted to implants and passive fit of one-piece cast frameworks fabricated with 3 different materials: commercially pure titanium (G1-CP Ti), cobalt-chromium alloy (G2-Co-Cr), and nickel-chromium-titanium alloy (G3-Ni-Cr-Ti). In total, 12 frameworks simulating bars for fixed prosthesis in a model with 5 implants were fabricated. The passive fit of the framework interface was measured using an optical microscope and the stresses transmitted to implants were measured using quantitative photoelastic analysis. Data were statistically analyzed by analysis of variance (ANOVA) and least significant difference (LSD) tests (α = 0.05). Mean and standard deviation values of passive fit and stress over implants are presented, respectively: G1 [472.49 (109.88) µm and 11.38 (9.23) KPa], G2 [584.84 (120.20) µm and 15.83 (9.30) KPa], and G3 [462.70 (179.18) µm and 16.39 (9.51) KPa]. For stress over implants, there were significant differences between G1, G2, and G3 (P = 0.035), being the lowest values for the G1. There were no significant differences for passive fit between G1 and G3 (P = 0.844), but both were statistically different from G2 (P = 0.028 and P = 0.035, respectively), which showed the worse results. It may be concluded that the stress over implants was affected by the tested materials. The CP Ti presented the best values for the evaluated items.

Marginal fit and microbial leakage along the implant-abutment interface of fixed partial prostheses: An in vitro analysis using Checkerboard DNA-DNA hybridization.

STATEMENT OF PROBLEM: Bidirectional leakage through the implant-abutment interface still constitutes a major concern in implant-supported restorations. PURPOSE: The purpose of this in vitro study was to evaluate the marginal fit, before and after loading simulation, of 3-unit fixed partial prostheses supported by external hexagon or Morse cone implants and to identify and quantify up to 43 microbial species penetrating through the implant-abutment interface after loading. MATERIAL AND METHODS: Forty-eight dental implants with external hexagon (EH; n=24) or Morse cone (MC; n=24) connections were investigated. Experimental specimens were made from 2 implants restored with a 3-unit fixed partial prosthesis and divided into 2 groups (n=12) according to platform connection EH or MC. Vertical misfit at the implant-abutment interface was measured before and after loading (150 Ncm during 500,000 cycles at 1.8 Hz). checkerboard DNA-DNA hybridization was used to identify and quantify up to 38 bacterial and 5 Candida species colonizing the internal parts of the implants after loading. Generalized estimating equations were used for statistical analysis (α=.05). RESULTS: The mean values (mm, ±SD) of vertical misfit for EH were 0.0131 ±0.002 before loading and 0.0138 ±0.002 after loading and for MC were 0.0132 ±0.003 before loading and 0.0137 ±0.001 after loading. Twenty-one bacterial species, including periodontal pathogens and C. albicans, were found colonizing the inner surfaces of EH implants after loading. None of the target species were detected in the internal parts of MC implants. CONCLUSIONS: EH implants showed higher microbial counts than MC implants, in which microbial colonization was not found after loading. Detected species included nonpathogens and microorganisms related to periodontal/periimplant diseases. Further studies are needed to evaluate the effect of loading simulation on the marginal misfit of 3-unit fixed partial prostheses supported by EH or MC implants, because no significant differences could be found either before or after loading.

Three-dimensional finite element analysis of endodontically treated teeth with weakened radicular walls restored with different protocols.

STATEMENT OF PROBLEM: The restoration of weakened roots with glass fiber posts (GFPs) remains a challenge. PURPOSE: The purpose of this study was to evaluate the stress distribution of endodontically treated teeth with weakened radicular walls restored with different protocols by 3-dimensional finite element analysis (3D-FEA). MATERIAL AND METHODS: The following 4 models of endodontically treated maxillary canines restored with metal ceramic crowns were simulated on the basis of computed microtomographic images to characterize the groups: GNW (control), a nonweakened root restored with a GFP; GW, a weakened root restored with a GFP; GDA, a weakened root restored with a direct anatomic GFP; and GIA, a weakened root restored with an indirect anatomic GFP. Loads of 180 N were applied to the lingual surface on the incisal third of the teeth at 45 degrees. The models were supported by a periodontal ligament and fixed in 3 axes (x=y=z=0). The von Mises stress (VMS) was calculated. RESULTS: All models exhibited VMS concentrations at the loading area and were distributed along the proximal surfaces of the root. The GFP exhibited a homogeneous stress distribution, and similar VMS distributions were observed in all of the reconstructive techniques. CONCLUSIONS: Similar stress distributions were observed in the endodontically treated teeth, regardless of root weakness and the reconstructive technique for the radicular walls.

Torque loss under mechanical cycling of long-span zirconia and titanium-cemented and screw-retained implant-supported CAD/CAM frameworks.

OBJECTIVES: This study evaluated the screw joint stability after cyclic loading of implant-supported titanium and zirconia CAD/CAM frameworks for fixed dental prostheses (FDPs) with different retention methods. MATERIAL AND METHODS: Twenty four one-piece frameworks supported by six threaded implants placed in the maxilla were fabricated using a CAD/CAM technique (NeoShape). Dry-pressed porcelain crowns were luted to the frameworks to standardize the specimens. The specimens were then divided into four groups (n = 6) according to framework material (titanium or zirconia) and retention method for the prosthesis (cement- or screw-retained): G1, Ti-cemented; G2, Ti-screw-retained; G3, Zr-cemented; and G4, Zr-screw-retained. A digital torque ratchet was used to assess the initial preload removal torque. Torque was then reapplied and the specimens were submitted to a 200 N cyclic load, at a frequency of 2 Hz, underwater in controlled temperature of 37°, and for 1 × 106 cycles. An opposing lower dental arch was fabricated using bis-acrylic resin to simulate occlusal contacts in centric. After cyclic loading, postload removal torque was measured. Preload and postload torque loss was expressed as a percentage of the initial load. Data were submitted to a linear mixed-effects model for statistical significance (α = 0.05) to evaluate the effect of cyclic loading in the screw torque loss used with frameworks of different materials and retention methods. RESULTS: Significant screw torque loss (%) was found for the tested groups (before/after cyclic loading, respectively): G1 (39.77/61.83), G2 (37.57/50.96), G3 (34.87/54.10), and G4 (47.56/73.50) (P < 0.05). CONCLUSIONS: The screw removal torque was significantly reduced for all groups in this study after cyclic loading the specimens. Screw-retained zirconia specimens presented the highest torque loss before and after the cyclic loadings compared with the other specimens that were tested.

Bacterial adhesion on the titanium and zirconia abutment surfaces.

OBJECTIVE: Microorganisms harboring the oral cavity, mainly those related to periodontal diseases, are the most potential etiologic factor of failure in long-term implant treatment. The material used for abutment components may influence the adhesion and colonization of microbial species. The aim of this in vivo investigation was to evaluate the biofilm formation on machined (MPT) or cast titanium (CPT) and zirconia abutments (Zc). METHODS: Six healthy subjects were enrolled in this randomized crossover clinical investigation. The study was conducted in three phases according to abutment surface evaluated. Each subject used an individual oral splint containing four disks of the same tested substrate, two located in the anterior and two in the posterior region, totalizing 12 specimens for subject. Participants were asked to use the removable intraoral splint during 24 h. DNA checkerboard hybridization method was used to identify and quantify 38 bacterial species colonizing formed biofilm on the abutment substrates. RESULTS: Pathogens and non-pathogens species were found colonizing the three substrates surfaces. Fusobacterium nucleatum, Neisseria mucosa, Porphyromonas aeruginosa, Peptostreptococcus anaerobios, Staphylococcus aureus, Streptococcus gordonii, Streptococcus parasanguinis, and Tanerella forsythia were the only species with no significant differences over the tested materials (P > 0.05). All the other target species presented significant differences sought by Friedman test (P < 0.0001). CONCLUSIONS: There was a significant difference in the total bacterial count between the three groups. CPT presented the higher mean counts, followed by MPT and Zc. CPT group also showed a higher mean incidence of species than MPT and Zc. The anterior or posterior region of disks placement did not show significant differences in relation to bacterial adhesion.

Correlation between vertical misfits and stresses over implants from castable frameworks made of different alloys.

This study aims to investigate a possible correlation between vertical misfits and the stresses transmitted to implants from one-piece casted frameworks fabricated with 3 different materials: commercially pure titanium, cobalt chromium alloy, and nickel chromium titanium alloy. Twelve frameworks simulating screw-retained prosthesis were fabricated from a master cast with 5 implants. Each framework was screwed (20 Ncm) over a metal cast and the vertical mesial and distal misfits were measured using an optical microscope. The stresses transmitted to the implants were measured in a third model by a quantitative photoelastic analysis. Stress and vertical misfit data were statistically analyzed by ANOVA and least significant difference tests and the correlation tests were performed using Pearson Correlation Test (α = 0.05). Mean and standard deviation values of vertical misfit and stress over implants are presented, respectively: commercially pure titanium (29.09 ± 13.24 µm and 11.38 ± 9.23 kPa), cobalt chromium alloy (27.05 ± 10.30 µm and 15.83 ± 9.30 kPa), nickel chromium titanium alloy (24.95 ± 11.14 µm and 16.39 ± 9.51 kPa). There were no significant differences for vertical misfit (P = 0.285). Regarding the stress analysis, there were significant differences between commercially pure titanium, cobalt chromium alloy, and nickel chromium titanium alloy (P = 0.035), with the lowest values for the commercially pure titanium. It may be concluded that stress over implants was affected by different procedures and materials for framework production.

Effect of cyclic loading on the vertical microgap of long-span zirconia frameworks supported by 4 or 6 implants.

STATEMENT OF PROBLEM: Few studies have investigated the microgap of long-span complete-arch fixed dental prosthesis zirconia frameworks. PURPOSE: The purpose of this study was to evaluate the effects of cyclic loading on the vertical microgap of maxillary 12-unit prostheses supported by 4 implants and on 14-unit prostheses supported by 6 implants. MATERIAL AND METHODS: One-piece zirconia frameworks were fabricated with a computer-aided design/computer-aided manufacturing technique and divided into 2 groups (n=5): a group of 12-unit prostheses and a group of 14-unit prostheses. The vertical microgap between the frameworks and prosthetic abutments was measured with an optical microscope (80×) under 2 reading conditions. Condition 1 (1-screw test): 1A, the screw on implant 1 was tightened and readings were made on all implants; 1B, the screw was changed to implant 4 (implant 6 for the 14-unit group) and readings were made on all implants. Condition 2: the microgap was measured with all screws tightened before cyclic loading (2A). Specimens were submitted to 200 N underwater (37°C) cyclic loading at a 2-Hz frequency for 1×10(6) cycles. Microgap reading condition 2 was repeated after cyclic loading (2B). The data were submitted to a linear mixed-effects model for statistical comparison (α=.05). RESULTS: A lower (P<.05) vertical microgap (µm) was found for the 12-unit group (reading conditions: 1A, 47.93; 1B, 43.83; 2A, 11.77; and 2B, 11.25) compared to the 14-unit group (1A, 94.87; 1B, 112.56; 2A, 21.28; and 2B, 16.90). No differences were found when each group was compared before and after cyclic loading (P>.05). CONCLUSIONS: The vertical microgap was significantly reduced after tightening all the screws in the framework, possibly leading to a nonpassive situation. Longer-span frameworks showed an increased microgap. Cyclic loading had no influence on the vertical microgap within each group.

Microbial Leakage through Three Different Implant-Abutment Interfaces on Morse Taper Implants In Vitro.

The objective of this study was to evaluate microbial leakage by means of genome counts, through the implant-abutment interface in dental implants with different Morse taper abutments. Fifty-six samples were prepared and divided in four groups: CMC TB (14 Cylindrical Implants-14 TiBase Abutments), CMX TB (14 Conical Implants-14 TiBase Abutments), CMX PU (14 Conical Implants-14 Universal Abutment) and CMX U (14 Tapered Implants-14 UCLA Abutments). Assemblies had their interface submerged in saliva as the contaminant. Samples were subjected either to thermomechanical cycling (2 × 106 mechanical cycles with frequency of 5 Hz and load of 120 N simultaneously with thermal cycles of 5-55 °C) or thermal cycling (5-55 °C). After cycling, the contents from the inner parts of assemblies were collected and analyzed using the Checkerboard DNA-DNA hybridization technique. Significant differences in the total genome counts were found after both thermomechanical or thermal cycling: CMX U > CMX PU > CMX TB > CMC TB. There were also significant differences in individual bacterial counts in each of the groups (p < 0.05). Irrespective of mechanical cycling, the type of abutment seems to influence not only the total microbial leakage through the interface, but also seems to significantly reflect differences considering individual target species.