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1.
J Microsc ; 258(2): 127-39, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-25639642

RESUMEN

In this paper, the use of lithium fluoride (LiF) as imaging radiation detector to analyse living cells by single-shot soft X-ray contact microscopy is presented. High resolved X-ray images on LiF of cyanobacterium Leptolyngbya VRUC135, two unicellular microalgae of the genus Chlamydomonas and mouse macrophage cells (line RAW 264.7) have been obtained utilizing X-ray radiation in the water window energy range from a laser plasma source. The used method is based on loading of the samples, the cell suspension, in a special holder where they are in close contact with a LiF crystal solid-state X-ray imaging detector. After exposure and sample removal, the images stored in LiF by the soft X-ray contact microscopy technique are read by an optical microscope in fluorescence mode. The clear image of the mucilaginous sheath the structure of the filamentous Leptolyngbya and the visible nucleolus in the macrophage cells image, are noteworthiness results. The peculiarities of the used X-ray radiation and of the LiF imaging detector allow obtaining images in absorption contrast revealing the internal structures of the investigated samples at high spatial resolution. Moreover, the wide dynamic range of the LiF imaging detector contributes to obtain high-quality images. In particular, we demonstrate that this peculiar characteristic of LiF detector allows enhancing the contrast and reveal details even when they were obscured by a nonuniform stray light.


Asunto(s)
Fluoruros , Compuestos de Litio , Microscopía/métodos , Animales , Chlamydomonas/ultraestructura , Cianobacterias/ultraestructura , Rayos Láser , Macrófagos/ultraestructura , Ratones , Células RAW 264.7 , Rayos X
2.
Phys Rev Lett ; 107(6): 065004, 2011 Aug 05.
Artículo en Inglés | MEDLINE | ID: mdl-21902333

RESUMEN

Fast electrons produced by a 10 ps, 160 J laser pulse through laser-compressed plastic cylinders are studied experimentally and numerically in the context of fast ignition. K(α)-emission images reveal a collimated or scattered electron beam depending on the initial density and the compression timing. A numerical transport model shows that implosion-driven electrical resistivity gradients induce strong magnetic fields able to guide the electrons. The good agreement with measured beam sizes provides the first experimental evidence for fast-electron magnetic collimation in laser-compressed matter.

3.
J Microsc ; 229(Pt 3): 490-5, 2008 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-18331500

RESUMEN

In this work, we report a method to observe soft X-ray radiographs at nanoscale of various kind of samples, biological and metallic, stored in a thin layer of lithium fluoride, employing scanning near-field optical microscopy with an optical resolution that reaches 50 nm. Lithium fluoride material works as a novel image detector for X-ray nano-radiographs, due to the fact that extreme ultraviolet radiation and soft X-rays efficiently produce stable point defects emitting optically stimulated visible luminescence in a thin surface layer. The bi-dimensional distribution of the so-created defects depends on the local nanostructure of the investigated sample.


Asunto(s)
Fluoruros , Compuestos de Litio , Microscopía de Sonda de Barrido , Radiografía , Cristalización , Microscopía Confocal , Microscopía Fluorescente , Nanotecnología/instrumentación , Nanotecnología/métodos , Olea/ultraestructura , Polen/ultraestructura , Radiografía/instrumentación , Radiografía/métodos , Rayos X
4.
Phys Rev E ; 95(6-1): 063205, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28709273

RESUMEN

Multimegabar laser-driven shock waves are unique tools for studying matter under extreme conditions. Accurate characterization of shocked matter is for instance necessary for measurements of equation of state data or opacities. This paper reports experiments performed at the LULI facility on the diagnosis of shock waves, using x-ray-absorption radiography. Radiographs are analyzed using standard Abel inversion. In addition, synthetic radiographs, which also take into account the finite size of the x-ray source, are generated using density maps produced by hydrodynamic simulations. Reported data refer to both plane cylindrical targets and hemispherical targets. Evolution and deformation of the shock front could be followed using hydrodynamic simulations.

5.
Rev Sci Instrum ; 87(1): 013504, 2016 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-26827318

RESUMEN

In this paper, a preliminary shadowgraph-based analysis of dust particles re-suspension due to loss of vacuum accident (LOVA) in ITER-like nuclear fusion reactors has been presented. Dust particles are produced through different mechanisms in nuclear fusion devices, one of the main issues is that dust particles are capable of being re-suspended in case of events such as LOVA. Shadowgraph is based on an expanded collimated beam of light emitted by a laser or a lamp that emits light transversely compared to the flow field direction. In the STARDUST facility, the dust moves in the flow, and it causes variations of refractive index that can be detected by using a CCD camera. The STARDUST fast camera setup allows to detect and to track dust particles moving in the vessel and then to obtain information about the velocity field of dust mobilized. In particular, the acquired images are processed such that per each frame the moving dust particles are detected by applying a background subtraction technique based on the mixture of Gaussian algorithm. The obtained foreground masks are eventually filtered with morphological operations. Finally, a multi-object tracking algorithm is used to track the detected particles along the experiment. For each particle, a Kalman filter-based tracker is applied; the particles dynamic is described by taking into account position, velocity, and acceleration as state variable. The results demonstrate that it is possible to obtain dust particles' velocity field during LOVA by automatically processing the data obtained with the shadowgraph approach.

6.
Rev Sci Instrum ; 85(6): 063112, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24985804

RESUMEN

Recently, surveying large areas in an automatic way, for early detection of both harmful chemical agents and forest fires, has become a strategic objective of defence and public health organisations. The Lidar and Dial techniques are widely recognized as a cost-effective alternative to monitor large portions of the atmosphere. To maximize the effectiveness of the measurements and to guarantee reliable monitoring of large areas, new data analysis techniques are required. In this paper, an original tool, the Universal Multi Event Locator, is applied to the problem of automatically identifying the time location of peaks in Lidar and Dial measurements for environmental physics applications. This analysis technique improves various aspects of the measurements, ranging from the resilience to drift in the laser sources to the increase of the system sensitivity. The method is also fully general, purely software, and can therefore be applied to a large variety of problems without any additional cost. The potential of the proposed technique is exemplified with the help of data of various instruments acquired during several experimental campaigns in the field.

8.
Phys Rev A Gen Phys ; 37(6): 2007-2011, 1988 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-9899893
9.
Phys Rev A ; 45(6): 4064-4076, 1992 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-9907458
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