RESUMEN
In this brief review, I attempt an overview of the main components of anatomical laminar-level connectivity. These are: extrinsic outputs, excitatory and inhibitory intrinsic connectivity, and intrinsic inputs. Supporting data are biased from the visual system of nonhuman primates (NHPs), but I have drawn as much as possible from a broader span in order to treat the important issue of area-specific variability. In a second part, I briefly discuss laminar connectivity in the context of network organization (feedforward/feedback cortical connections, and the major types of corticothalamic connections). I also point out anatomical issues in need of clarification, including more systematic, whole brain coverage of tracer injections; more data on anterogradely labeled terminations; more complete, area-specific quantitative data about projection neurons, and quantitative data on terminal density and convergence. Postsynaptic targets are largely unknown, but their identification is essential for understanding the finer analysis and principles of laminar patterns. Laminar resolution MRI offers a promising new tool for exploring laminar connectivity: it is potentially fast and macro-scale, and allows for repeated investigation under different stimulus conditions. Conversely, anatomical resolution, although detailed beyond the current level of MRI visualization, offers a rich trove for experimental design and interpretation of fMRI activation patterns.
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Encéfalo/anatomía & histología , Vías Nerviosas/anatomía & histología , Animales , Neuroimagen Funcional/métodos , Humanos , Imagen por Resonancia Magnética/métodosRESUMEN
Recent commentaries on the role of the thalamus consider a wide sphere of influence beyond sensory-motor transformation, to include task-relevant cognitive processes. In this short review, I reconsider known anatomic features of corticothalamic connectivity, primarily for macaque monkey, and discuss these as part of an intricate network architecture consistent with multiple connectional recombinations and a diversity of functional tasks. Drawing mainly on results from single axon analysis for the two broad classes of corticothalamic (CT) connections, I review the strikingly complementary spatial parameters of their extrinsic CT arbors in relation to intrinsic cortical collaterals. That is, CT neurons in layer 5 (class II) have spatially compact (low divergent) thalamic fields, but highly spatially divergent cortical collaterals. In contrast, CT neurons in layer 6 (class I) have highly divergent thalamic fields, but delimited, low divergent cortical collaterals. CT convergence in the thalamus is technically more difficult to analyze, but one can infer a low convergence of terminations from layer 5, in contrast with CT terminations from layer 6, which are highly convergent. Reciprocating thalamocortical (TC) axons have multiple clustered and divergent arbors. What to conclude from these relationships requires further investigation of activity patterns and networks under different conditions. Specific parameters are suggestive of selective recruitment of distributed postsynaptic networks and ordered activity sequences; but are these separable systems, operating cooperatively or in parallel (L.5 low divergent/low convergent vs. L. 6 high divergent/high convergent)?
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Axones , Corteza Cerebral/citología , Tálamo/citología , Animales , Macaca , Vías Nerviosas/citología , Técnicas de Trazados de Vías NeuroanatómicasRESUMEN
A systematic classification and accepted nomenclature of neuron types is much needed but is currently lacking. This article describes a possible taxonomical solution for classifying GABAergic interneurons of the cerebral cortex based on a novel, web-based interactive system that allows experts to classify neurons with pre-determined criteria. Using Bayesian analysis and clustering algorithms on the resulting data, we investigated the suitability of several anatomical terms and neuron names for cortical GABAergic interneurons. Moreover, we show that supervised classification models could automatically categorize interneurons in agreement with experts' assignments. These results demonstrate a practical and objective approach to the naming, characterization and classification of neurons based on community consensus.
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Algoritmos , Corteza Cerebral/citología , Interneuronas/clasificación , Interneuronas/citología , Terminología como Asunto , Ácido gamma-Aminobutírico/metabolismo , Animales , Teorema de Bayes , Corteza Cerebral/metabolismo , Análisis por Conglomerados , Humanos , Interneuronas/metabolismoRESUMEN
In this novel large-scale multiplexed immunofluorescence study we comprehensively characterized and compared layer-specific proteomic features within regions of interest of the widely divergent dorsolateral prefrontal cortex (A46) and primary visual cortex (A17) of adult rhesus monkeys. Twenty-eight markers were imaged in rounds of sequential staining, and their spatial distribution precisely quantified within gray matter layers and superficial white matter. Cells were classified as neurons, astrocytes, oligodendrocytes, microglia, or endothelial cells. The distribution of fibers and blood vessels were assessed by quantification of staining intensity across regions of interest. This method revealed multivariate similarities and differences between layers and areas. Protein expression in neurons was the strongest determinant of both laminar and regional differences, whereas protein expression in glia was more important for intra-areal laminar distinctions. Among specific results, we observed a lower glia-to-neuron ratio in A17 than in A46 and the pan-neuronal markers HuD and NeuN were differentially distributed in both brain areas with a lower intensity of NeuN in layers 4 and 5 of A17 compared to A46 and other A17 layers. Astrocytes and oligodendrocytes exhibited distinct marker-specific laminar distributions that differed between regions; notably, there was a high proportion of ALDH1L1-expressing astrocytes and of oligodendrocyte markers in layer 4 of A17. The many nuanced differences in protein expression between layers and regions observed here highlight the need for direct assessment of proteins, in addition to RNA expression, and set the stage for future protein-focused studies of these and other brain regions in normal and pathological conditions.
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Sustancia Gris , Macaca mulatta , Corteza Prefrontal , Proteómica , Sustancia Blanca , Animales , Sustancia Blanca/metabolismo , Sustancia Gris/metabolismo , Corteza Prefrontal/metabolismo , Neocórtex/metabolismo , Neocórtex/citología , Masculino , Astrocitos/metabolismo , Neuronas/metabolismo , Oligodendroglía/metabolismo , Neuroglía/metabolismo , Femenino , Lóbulo Occipital/metabolismo , Corteza Visual/metabolismoRESUMEN
The spontaneously hypertensive rat (SHR) is the most widely studied animal model of hypertension. Scores of SHR quantitative loci (QTLs) have been mapped for hypertension and other phenotypes. We have sequenced the SHR/OlaIpcv genome at 10.7-fold coverage by paired-end sequencing on the Illumina platform. We identified 3.6 million high-quality single nucleotide polymorphisms (SNPs) between the SHR/OlaIpcv and Brown Norway (BN) reference genome, with a high rate of validation (sensitivity 96.3%-98.0% and specificity 99%-100%). We also identified 343,243 short indels between the SHR/OlaIpcv and reference genomes. These SNPs and indels resulted in 161 gain or loss of stop codons and 629 frameshifts compared with the BN reference sequence. We also identified 13,438 larger deletions that result in complete or partial absence of 107 genes in the SHR/OlaIpcv genome compared with the BN reference and 588 copy number variants (CNVs) that overlap with the gene regions of 688 genes. Genomic regions containing genes whose expression had been previously mapped as cis-regulated expression quantitative trait loci (eQTLs) were significantly enriched with SNPs, short indels, and larger deletions, suggesting that some of these variants have functional effects on gene expression. Genes that were affected by major alterations in their coding sequence were highly enriched for genes related to ion transport, transport, and plasma membrane localization, providing insights into the likely molecular and cellular basis of hypertension and other phenotypes specific to the SHR strain. This near complete catalog of genomic differences between two extensively studied rat strains provides the starting point for complete elucidation, at the molecular level, of the physiological and pathophysiological phenotypic differences between individuals from these strains.
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Hipertensión/genética , Animales , Codón de Terminación , Dosificación de Gen , Polimorfismo de Nucleótido Simple , Sitios de Carácter Cuantitativo , Ratas , Ratas Endogámicas SHR , Transcripción GenéticaRESUMEN
Neuroscience produces a vast amount of data from an enormous diversity of neurons. A neuronal classification system is essential to organize such data and the knowledge that is derived from them. Classification depends on the unequivocal identification of the features that distinguish one type of neuron from another. The problems inherent in this are particularly acute when studying cortical interneurons. To tackle this, we convened a representative group of researchers to agree on a set of terms to describe the anatomical, physiological and molecular features of GABAergic interneurons of the cerebral cortex. The resulting terminology might provide a stepping stone towards a future classification of these complex and heterogeneous cells. Consistent adoption will be important for the success of such an initiative, and we also encourage the active involvement of the broader scientific community in the dynamic evolution of this project.
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Corteza Cerebral/citología , Interneuronas , Ácido gamma-Aminobutírico/metabolismo , Potenciales de Acción , Axones/ultraestructura , Corteza Cerebral/metabolismo , Humanos , Interneuronas/clasificación , Interneuronas/citología , Interneuronas/metabolismo , Sinapsis/ultraestructuraRESUMEN
The angular gyrus is associated with a spectrum of higher order cognitive functions. This mini-review undertakes a broad survey of putative neuroanatomical substrates, guided by the premise that area-specific specializations derive from a combination of extrinsic connections and intrinsic area properties. Three levels of spatial resolution are discussed: cellular, supracellular connectivity, and synaptic micro-scale, with examples necessarily drawn mainly from experimental work with nonhuman primates. A significant factor in the functional specialization of the human parietal cortex is the pronounced enlargement. In addition to "more" cells, synapses, and connections, however, the heterogeneity itself can be considered an important property. Multiple anatomical features support the idea of overlapping and temporally dynamic membership in several brain wide subnetworks, but how these features operate in the context of higher cognitive functions remains for continued investigations.
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Corteza Cerebral , Lóbulo Parietal , Animales , Humanos , Sinapsis , Cognición , EncéfaloRESUMEN
The feedforward/feedback classification, as originally stated in relation to early visual areas in the macaque monkey, has had a significant influence on ideas of laminar interactions, area reciprocity, and cortical hierarchical organization. In some contrast with this macroscale "laminar connectomics," a more cellular approach to cortical connections, as briefly surveyed here, points to a still underappreciated heterogeneity of neuronal subtypes and complex microcircuitries. From the perspective of heterogeneities, the question of how brain regions interact and influence each other quickly leads to discussions about concurrent hierarchical and nonhierarchical cortical features, brain organization as a multiscale system forming nested groups and hierarchies, connectomes annotated by multiple biological attributes, and interleaved and overlapping scales of organization.
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Conectoma , Corteza Visual , Animales , Corteza Visual/fisiología , Macaca/fisiología , Neuronas/fisiología , Encéfalo/fisiologíaRESUMEN
The inferior temporal (IT) cortex is the last unimodal visual area in the ventral visual pathway and is essential for color discrimination. Recent imaging and electrophysiological studies have revealed the presence of several distinct patches of color-selective cells in the anterior IT cortex (AIT) and posterior IT cortex (PIT). To understand the neural machinery for color processing in the IT cortex, in the present study, we combined anatomical tracing methods with electrophysiological unit recordings to investigate the anatomical connections of identified clusters of color-selective cells in monkey IT cortex. We found that a color cluster in AIT received projections from a color cluster in PIT as well as from discrete clusters of cells in other occipitotemporal areas, in the superior temporal sulcus, and in prefrontal and parietal cortices. The distribution of the labeled cells in PIT closely corresponded with that of the physiologically identified color-selective cells in this region. Furthermore, retrograde tracer injections in the posterior color cluster resulted in labeled cells in the anterior cluster. Thus, temporal lobe color-processing modules form a reciprocally interconnected loop within a distributed network.
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Mapeo Encefálico , Percepción de Color/fisiología , Lóbulo Temporal/fisiología , Vías Visuales/fisiología , Potenciales de Acción/fisiología , Animales , Biotina/análogos & derivados , Biotina/metabolismo , Toxina del Cólera/metabolismo , Color , Dextranos/metabolismo , Macaca fascicularis , Proteínas de Neurofilamentos/metabolismo , Neuronas/fisiología , Parvalbúminas/metabolismo , Estimulación Luminosa , Lóbulo Temporal/citología , Factores de TiempoRESUMEN
Pyramidal neurons with axons that exit from dendrites rather than the cell body itself are relatively common in non-primates, but rare in monkeys and humans.
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Axones , Neuronas , Axones/fisiología , Cuerpo Celular , Neuronas/fisiología , Células PiramidalesRESUMEN
The stria terminalis (ST) is a major bidirectional fiber tract anchored in the amygdala and bed nucleus (BNST). Extensive investigations in rodents report a complex arrangement of neurochemically diverse neurons within the ST, but fewer data are available for non-human primates. Given the functional importance of the ST, we investigated its microarchitecture in one newborn, four infant, and two adult macaque brains, by parallel immunocytochemical series for cells or fibers. Main results are as follows: (1) The pan-neuronal marker NeuN shows scattered neurons and small neuronal clusters in both the dorsal and ventral ST, but more numerous dorsally; (2) smaller neuronal subpopulations are labeled by calretinin (CR), neuropeptide Y (NPY), calbindin (CB), and somatostatin (SOM), of which the CR + neurons are the most numerous; (3) the infant brains on average have more neurons in the ST than the adult brains, but across our sample, there is notable individual variability; and (4) fiber architectonics have a complex organization, which can be referenced to myelin-poor or myelin-dense zones. Myelin-poor zones coincide with concentrations of fibers positive for CB, CR, or tyrosine hydroxylase (TH). Neurons have been reported in other white matter domains (e.g., anterior commissure, corpus callosum, cingulum bundle, and subcortical white matter). Like these, at least some neurons within the ST may give rise to long-distance connections, and/or participate in more local functions, such as vascular regulation or axon guidance/maintenance.
RESUMEN
Temporal lobe epilepsy (TLE) is the most common form of focal epilepsy and is associated with a variety of structural and psychological alterations. Recently, there has been renewed interest in using brain tissue resected during epilepsy surgery, in particular 'non-epileptic' brain samples with normal histology that can be found alongside epileptic tissue in the same epileptic patients - with the aim being to study the normal human brain organization using a variety of methods. An important limitation is that different medical characteristics of the patients may modify the brain tissue. Thus, to better determine how 'normal' the resected tissue is, it is fundamental to know certain clinical, anatomical and psychological characteristics of the patients. Unfortunately, this information is frequently not fully available for the patient from which the resected tissue has been obtained - or is not fully appreciated by the neuroscientists analyzing the brain samples, who are not necessarily experts in epilepsy. In order to present the full picture of TLE in a way that would be accessible to multiple communities (e.g., basic researchers in neuroscience, neurologists, neurosurgeons and psychologists), we have reviewed 34 TLE patients, who were selected due to the availability of detailed clinical, anatomical, and psychological information for each of the patients. Our aim was to convey the full complexity of the disorder, its putative anatomical substrates, and the wide range of individual variability, with a view toward: (1) emphasizing the importance of considering critical patient information when using brain samples for basic research and (2) gaining a better understanding of normal and abnormal brain functioning. In agreement with a large number of previous reports, this study (1) reinforces the notion of substantial individual variability among epileptic patients, and (2) highlights the common but overlooked psychopathological alterations that occur even in patients who become "seizure-free" after surgery. The first point is based on pre- and post-surgical comparisons of patients with hippocampal sclerosis and patients with normal-looking hippocampus in neuropsychological evaluations. The second emerges from our extensive battery of personality and projective tests, in a two-way comparison of these two types of patients with regard to pre- and post-surgical performance.
RESUMEN
The COUP-TFII nuclear receptor, also known as NR2F2, is expressed in the developing ventral telencephalon and modulates the tangential migration of a set of subpallial neuronal progenitors during forebrain development. Little information is available about its expression patterns in the adult brain. We have identified the cell populations expressing COUP-TFII and the contribution of some of them to network activity in vivo. Expression of COUP-TFII by hippocampal pyramidal and dentate granule cells, as well as neurons in the neocortex, formed a gradient increasing from undetectable in the dorsal to very strong in the ventral sectors. In the dorsal hippocampal CA1 area, COUP-TFII was restricted to GABAergic interneurons and expressed in several, largely nonoverlapping neuronal populations. Immunoreactivity was present in calretinin-, neuronal nitric oxide synthase-, and reelin-expressing cells, as well as in subsets of cholecystokinin- or calbindin-expressing or radiatum-retrohippocampally projecting GABAergic cells, but not in parvalbumin- and/or somatostatin-expressing interneurons. In vivo recording and juxtacellular labeling of COUP-TFII-expressing cells revealed neurogliaform cells, basket cells in stratum radiatum and tachykinin-expressing radiatum dentate innervating interneurons, identified by their axodendritic distributions. They showed cell type-selective phase-locked firing to the theta rhythm but no activation during sharp wave/ripple oscillations. These basket cells in stratum radiatum and neurogliaform cells fired at the peak of theta oscillations detected extracellularly in stratum pyramidale, unlike previously reported ivy cells, which fired at the trough. The characterization of COUP-TFII-expressing neurons suggests that this developmentally important transcription factor plays cell type-specific role(s) in the adult hippocampus.
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Factor de Transcripción COUP II/metabolismo , Hipocampo/citología , Hipocampo/metabolismo , Neuronas/metabolismo , Receptores de GABA/metabolismo , Aminas , Animales , Axones/metabolismo , Moléculas de Adhesión Celular Neuronal/metabolismo , Linaje de la Célula/genética , Ácidos Ciclohexanocarboxílicos , Proteínas de la Matriz Extracelular/metabolismo , Gabapentina , Interneuronas/metabolismo , Masculino , Red Nerviosa/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Neuroglía/metabolismo , Técnicas de Placa-Clamp , Células Piramidales/metabolismo , Ratas , Ratas Sprague-Dawley , Proteína Reelina , Serina Endopeptidasas/metabolismo , Ácido gamma-AminobutíricoRESUMEN
To investigate the fine anatomical organization of cortical inputs to visual association area TE, 2-3 small injections of retrograde tracers were made in macaque monkeys. Injections were made as a terminal procedure, after optical imaging and electrophysiological recording, and targeted to patches physiologically identified as object-selective. Retrogradely labeled neurons occurred in several unimodal visual areas, the superior temporal sulcus, intraparietal sulcus (IPS), and prefrontal cortex (PFC), consistent with previous studies. Despite the small injection size (<0.5 mm wide), the projection foci in visual areas, but not in IPS or PFC, were spatially widespread (4-6 mm in extent), and predominantly consisted of neurons labeled by only one of the injections. This can be seen as a quasi-modular organization. In addition, within each projection focus, there were scattered neurons projecting to one of the other injections, together with some double-labeled (DL) neurons, in a more distributed pattern. Finally, projection foci included smaller "hotspots," consisting of intermixed neurons, single-labeled by the different injections, and DL neurons. DL neurons are likely the result of axons having extended, spatially separated terminal arbors, as demonstrated by anterograde experiments. These results suggest a complex, hybrid connectivity architecture, with both modular and distributed components.
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Reconocimiento Visual de Modelos/fisiología , Lóbulo Temporal/anatomía & histología , Corteza Visual/anatomía & histología , Vías Visuales/anatomía & histología , Animales , Axones/fisiología , Axones/ultraestructura , Mapeo Encefálico , Electrofisiología , Técnica del Anticuerpo Fluorescente , Macaca mulatta , Masculino , Microscopía Fluorescente , Red Nerviosa/anatomía & histología , Red Nerviosa/fisiología , Trazadores del Tracto Neuronal , Neuronas/citología , Neuronas/fisiología , Lóbulo Parietal/fisiología , Estimulación Luminosa , Corteza Prefrontal/fisiología , Lóbulo Temporal/fisiología , Corteza Visual/fisiología , Vías Visuales/fisiología , Imagen de Colorante Sensible al VoltajeRESUMEN
Synaptic zinc is an activity-related neuromodulator, enriched in hippocampal mossy fibers and a subset of glutamatergic cortical projections, exclusive of thalamocortical or corticothalamic. Some degree of pathway specificity in the utilization of synaptic zinc has been reported in rodents. Here, we use focal injections of the retrograde tracer sodium selenite to identify zinc-positive (Zn+) projection neurons in the monkey ventral visual pathway. After injections in V1, V4, and TEO areas, neurons were detected preferentially in several feedback pathways but, unusually, were restricted to deeper layers without involvement of layers 2 or 3. Temporal injections resulted in more extensive labeling of both feedback and intratemporal association pathways. The Zn+ neurons had a broader laminar distribution, similar to results from standard retrograde tracers. After anterograde tracer injection in area posterior TE, electron microscopic analysis substantiated that a proportion of feedback synapses was co-labeled with zinc. Nearby injections, Zn+ intrinsic neurons concentrated in layer 2, but in temporal areas were also abundant in layer 6. These results indicate considerable pathway and laminar specificity as to which cortical neurons use synaptic zinc. Given the hypothesized roles of synaptic zinc, this is likely to result in distinct synaptic properties, possibly including differential synaptic plasticity within or across projections.
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Vías Nerviosas/metabolismo , Sinapsis/metabolismo , Corteza Visual/metabolismo , Zinc/metabolismo , Animales , Macaca , Microscopía Electrónica de Transmisión , Vías Nerviosas/ultraestructura , Sinapsis/ultraestructura , Corteza Visual/ultraestructuraRESUMEN
Apical dendritic bundles from pyramidal neurons are a prominent feature of cortical neuropil but with significant area specializations. Here, we investigate mechanisms of bundle formation, focusing on layer (L) 2 bundles in rat granular retrosplenial cortex (GRS), a limbic area implicated in spatial memory. By using microarrays, we first searched for genes highly and specifically expressed in GRS L2 at postnatal day (P) 3 versus GRS L2 at P12 (respectively, before and after bundle formation), versus GRS L5 (at P3), and versus L2 in barrel field cortex (BF) (at P3). Several genes, including neurotrophin-3 (NT-3), were identified as transiently and specifically expressed in GRS L2. Three of these were cloned and confirmed by in situ hybridization. To test that NT-3-mediated events are causally involved in bundle formation, we used in utero electroporation to overexpress NT-3 in other cortical areas. This produced prominent bundles of dendrites originating from L2 neurons in BF, where L2 bundles are normally absent. Intracellular biocytin fills, after physiological recording in vitro, revealed increased dendritic branching in L1 of BF. The controlled ectopic induction of dendritic bundles identifies a new role for NT-3 and a new in vivo model for investigating dendritic bundles and their formation.
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Envejecimiento , Dendritas/fisiología , Sistema Límbico/metabolismo , Neuronas/metabolismo , Neurotrofina 3/genética , Neurotrofina 3/metabolismo , Corteza Somatosensorial/metabolismo , Envejecimiento/fisiología , Animales , Procesos de Crecimiento Celular , Sistema Límbico/citología , Masculino , Neuronas/citología , Análisis de Secuencia por Matrices de Oligonucleótidos , Ratas , Corteza Somatosensorial/citología , Regulación hacia Arriba/genéticaRESUMEN
An ordered relation of structure and function has been a cornerstone in thinking about brain organization. Like the brain itself, however, this is not straightforward and is confounded both by functional intricacy and structural plasticity (many routes to a given outcome). As a striking case of putative structure-function correlation, this mini-review focuses on the relatively well-characterized pattern of cytochrome oxidase (CO) blobs (aka "patches" or "puffs") in the supragranular layers of macaque monkey visual cortex. The pattern is without doubt visually compelling, and the semi-dichotomous array of CO+ blobs and CO- interblobs is consistent with multiple studies reporting compartment-specific preferential connectivity and distinctive physiological response properties. Nevertheless, as briefly reviewed here, the finer anatomical organization of this system is surprisingly under-investigated, and the relation to functional aspects, therefore, unclear. Microcircuitry, cell type, and three-dimensional spatiotemporal level investigations of the CO+ CO- pattern are needed and may open new views to structure-function organization of visual cortex, and to phylogenetic and ontogenetic comparisons across nonhuman primates (NHP), and between NHP and humans.
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Complejo IV de Transporte de Electrones , Corteza Visual , Animales , Complejo IV de Transporte de Electrones/metabolismo , Macaca , Filogenia , Corteza Visual/metabolismoRESUMEN
We examined the number, distribution, and immunoreactivity of the infracortical white matter neuronal population, also termed white matter interstitial cells (WMICs), throughout the telencephalic white matter of an adult female chimpanzee. Staining for neuronal nuclear marker (NeuN) revealed WMICs throughout the infracortical white matter, these cells being most numerous and dense close to the inner border of cortical layer VI, decreasing significantly in density with depth in the white matter. Stereological analysis of NeuN-immunopositive cells revealed an estimate of approximately 137.2 million WMICs within the infracortical white matter of the chimpanzee brain studied. Immunostaining revealed subpopulations of WMICs containing neuronal nitric oxide synthase (nNOS, approximately 14.4 million in number), calretinin (CR, approximately 16.7 million), very few WMICs containing parvalbumin (PV), and no calbindin-immunopositive neurons. The nNOS, CR, and PV immunopositive WMICs, possibly all inhibitory neurons, represent approximately 22.6% of the total WMIC population. As the white matter is affected in many cognitive conditions, such as schizophrenia, autism, epilepsy, and also in neurodegenerative diseases, understanding these neurons across species is important for the translation of findings of neural dysfunction in animal models to humans. Furthermore, studies of WMICs in species such as apes provide a crucial phylogenetic context for understanding the evolution of these cell types in the human brain.
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Corteza Cerebral/fisiología , Neuronas/química , Pan troglodytes/fisiología , Sustancia Blanca/fisiología , Animales , Química Encefálica , Calbindina 2/metabolismo , Calbindinas/metabolismo , Recuento de Células , Corteza Cerebral/química , Corteza Cerebral/citología , Femenino , Inmunohistoquímica , Modelos Animales , Óxido Nítrico Sintasa de Tipo I/metabolismo , Parvalbúminas/metabolismo , Sustancia Blanca/química , Sustancia Blanca/citologíaRESUMEN
Anterogradely labeled connections at the single-axon level provide unparalleled spatial and quantitative data as well as a novel perspective on laminar, columnar, hierarchical and other aspects of cortical organization. Here, I briefly summarize single-axon results from representative examples of thalamocortical, corticocortical, callosal, and lateral intrinsic connections, with attention to implications for cortical organization. Particularly worth emphasizing is the intricate spatial configuration and striking morphometric heterogeneity of individual axons even within the same system of connections. A short section touches on patterns of axonal trajectories in the distal, preterminal few millimeters. Emphasis is on studies in nonhuman primates from about 1983 to present, with non-viral tracers and 2-D reconstruction (i.e., compressed z-axis) in the early visual cortical pathway. The last section recapitulates what this approach can tell us about inter-areal communication and cortical organization, and possible implications for dynamics and effective connectivity, and concludes with comments on open questions and future directions.
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Axones , Encéfalo/citología , Animales , Corteza Cerebral/citología , Cuerpo Calloso/citología , Vías Nerviosas/citología , Técnicas de Trazados de Vías Neuroanatómicas , Primates , Tálamo/citologíaRESUMEN
Continuing investigations of corticostriatal connections in rodents emphasize an intricate architecture where striatal projections originate from different combinations of cortical layers, include an inhibitory component, and form terminal arborizations which are cell-type dependent, extensive, or compact. Here, we report that in macaque monkeys, deep and superficial cortical white matter neurons (WMNs), peri-claustral WMNs, and the claustrum proper project to the putamen. WMNs retrogradely labeled by injections in the putamen (four injections in three macaques) were widely distributed, up to 10 mm antero-posterior from the injection site, mainly dorsal to the putamen in the external capsule, and below the premotor cortex. Striatally projecting labeled WMNs (WMNsST) were heterogeneous in size and shape, including a small GABAergic component. We compared the number of WMNsST with labeled claustral and cortical neurons and also estimated their proportion in relation to total WMNs. Since some WMNsST were located adjoining the claustrum, we wanted to compare results for density and distribution of striatally projecting claustral neurons (ClaST). ClaST neurons were morphologically heterogeneous and mainly located in the dorsal and anterior claustrum, in regions known to project to frontal, motor, and cingulate cortical areas. The ratio of ClaST to WMNsST was about 4:1 averaged across the four injections. These results provide new specifics on the connectional networks of WMNs in nonhuman primates, and delineate additional loops in the corticostriatal architecture, consisting of interconnections across cortex, claustralstriatal and striatally projecting WMNs.