RESUMEN
Seasonal outbreaks of acute encephalitis syndrome (AES) at Gorakhpur, India have been recognized since 2006. So far, the causative agent has not been identified. Use of next generation sequencing identified human parvovirus 4 (HPARV4) sequences in a CSF/plasma pool. These sequences showed highest identity with sequences earlier identified in similar patients from south India. Real-time PCR detected HPARV4 DNA in 20/78 (25.6%) CSF and 6/31 (19.3%) plasma of AES patients. Phylogenetic analysis classified three almost complete genomes and 24 partial NS1 sequences as genotype 2A. The observed association of HPARV4 with AES needs further evaluation. ELISAs for the detection of IgM and IgG antibodies against scrub typhus (Orientia tsutsugamushi, OT) showed â¼70% IgM/IgG positivity suggestive of etiologic association. Prospective, comprehensive studies are needed to confirm association of these agents, singly or in combination with AES in Gorakhpur region.
Asunto(s)
Encefalopatía Aguda Febril/virología , Brotes de Enfermedades , Infecciones por Parvoviridae/epidemiología , Parvovirus/aislamiento & purificación , Análisis de Secuencia de ADN/métodos , Encefalopatía Aguda Febril/sangre , Encefalopatía Aguda Febril/líquido cefalorraquídeo , Encefalopatía Aguda Febril/epidemiología , Niño , Preescolar , ADN Viral/genética , Femenino , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , India/epidemiología , Lactante , Masculino , Infecciones por Parvoviridae/sangre , Infecciones por Parvoviridae/líquido cefalorraquídeo , Infecciones por Parvoviridae/diagnóstico , Parvovirus/genética , Parvovirus/inmunología , FilogeniaRESUMEN
Human herpes simplex virus (HSV)-1 infection is acquired in childhood and persists throughout a person's lifetime. Here, we present two cases from India; one showing symptoms of postpartum haemorrhage with disseminated intravascular coagulation, and the second one showing signs of acute encephalitis syndrome. The aetiological agent in both cases was identified as HSV-1 using the PCR method. The next-generation sequencing method retrieved ~97â% of the viral genome from the isolates of the clinical samples. The phylogenetic analysis of the retrieved genomes revealed that they belong to clade II of HSV-1. This study identifies a few sequence variations in the glycoprotein region of HSV-1 during two different clinical manifestations. There are a couple of papers that analyse variations in the glycoprotein region of clinical samples. Further, this study also highlights the importance of considering HSV-1 during differential diagnosis when analysing the nosocomial infection.