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The pre-cervical uterine torsion is a rotation of a pregnant uterine horn on its longitudinal axis with the point of rotation located cranial to the cervix. After diagnosis based on vaginal and rectal examination, uterine detorsion was first transrectal approached because the cervix was not open and blocked. Five cases are described. The direction of rotation in all 5 cows was clockwise. The head of the calf was manually caught with the left hand per rectum and the entire uterus swayed several times in a left-right direction. During left-right swinging of the pregnant uterus and fetus, manually through the rectum, when the right swing tip reached, then abruptly tried to turn anti-clockwise. The procedure was repeated several times. After partial transrectal detorsion, we could access the calf, vaginally, and detorsion with the right hand and the same procedure of swinging the calf were started, but vaginally. Even if the uterus was restored to the normal position, sometimes the calf remained in an unfavourable position; therefore, it was corrected in the dorsal position and prepared for a forced extraction. This is the first report of a successful combination of transrectal and vaginal manual detorsion of a rare pre-cervical uterine torsion in cows.
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Útero , Vagina , Embarazo , Femenino , Animales , Bovinos , Útero/diagnóstico por imagen , PelvisRESUMEN
The dairy sector is essential for the world's food systems, playing a vital role in agricultural production. Yet, mastitis, an inflammation of the mammary gland, affects a considerable portion of the dairy cow population annually, leading to reduced productivity and sustainability. Insight into therapeutic protocols is essential for a better understanding of the situation on farms with the aim of developing new or harmonizing existing protocols in the mastitis treatment. This study conducted on dairy farm in Serbia aimed to explore the most commonly used antibiotics during bovine mastitis treatment and their association with daily milk yield. Data from 100 lactating cows, including therapeutic protocols, lactation numbers, mastitis episodes, milk yield, and antibiotic usage, were obtained from a computerized database and statistically analysed. The results demonstrated a high prevalence of narrow-spectrum antibiotics usage, with the majority of cows treated with combination therapy. The route of antibiotic application did not significantly impact daily milk yield, while treatment duration had no discernible effect. Nonetheless, specific antibiotics were associated with milk yield variations, suggesting the need for careful antibiotic selection and management in mastitis therapy. These findings emphasize the importance of prudent antibiotic use to safeguard animal health and long-term milk production sustainability.
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Enfermedades de los Bovinos , Mastitis Bovina , Femenino , Animales , Bovinos , Lactancia , Antibacterianos/uso terapéutico , Mastitis Bovina/tratamiento farmacológico , Serbia/epidemiología , Glándulas Mamarias Animales , Leche , Industria Lechera , Recuento de Células/veterinaria , Enfermedades de los Bovinos/tratamiento farmacológicoRESUMEN
Dilated cardiomyopathy is one of the important diseases in dogs and humans. The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. This study aimed to determine changes in the plasma metabolome of dogs with iDCM compared to healthy dogs. For that purpose, a multiplatform mass-spectrometry-based approach was used. In this study, we included two groups of dogs: 12 dogs with iDCM and 8 healthy dogs. A total of 272 metabolites were detected in the plasma samples of dogs by combining three approaches but four MS-based platforms (GC-MS, LC-MS (untargeted), LC-MS (targeted), and FIA-MS (targeted) methods). Our findings demonstrated changes in the canine plasma metabolome involved in the development of iDCM, including the different concentrations of amino acids, biogenic amines, acylcarnitines, triglycerides and diglycerides, sphingomyelins, and organic acids. The results of this study will enable the detection and monitoring of pathophysiological mechanisms involved in the development of iDCM in the future.
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Cardiomiopatía Dilatada , Insuficiencia Cardíaca , Humanos , Perros , Animales , Cardiomiopatía Dilatada/metabolismo , Metaboloma , Aminoácidos/metabolismo , Cromatografía de Gases y Espectrometría de MasasRESUMEN
When choosing a voluntary blood donor (VBD), it is important to ensure the donor health, and at the same time to produce a quality and safe blood product. The donor selection process leads to donor rejection related to their current health condition. The aim of this study was to determine gender differences in VBDs, to examine the reasons for their rejection, and to determine the share of permanently and temporarily deferred VBD, especially due to low hemoglobin levels. The research was conducted in eastern Croatia in the 2014 to 2018 period. The study included 144,041 blood donations from a total of 80,418 VBDs, of which 83.3% of donations were from male VBDs and 16.7% from female VBDs. There were 11.46% of temporarily deferred and permanently rejected donors. Out of all temporarily deferred donors and possible reasons for deferral, the largest share of deferrals referred to a reduced hemoglobin level, which accounted for 54% in female VBD and 46% in male VBD. Female VBDs made up to one-third of total VBDs and account for one-sixth of total blood donations. Low hemoglobin was the most common reason for VBD deferral. Gender and age are related to blood hemoglobin levels in rejected and accepted donors. Female VBDs represent a potential reservoir for increasing the total number of VBDs.
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Donantes de Sangre , Hemoglobinas , Humanos , Masculino , Femenino , Croacia , Hemoglobinas/análisis , Selección de Donante , Factores SexualesRESUMEN
Herniation of the intervertebral disc (IVDH) is the most common cause of neurological and intervertebral disc degeneration-related diseases. Since the disc starts to degenerate before it can be observed by currently available diagnostic methods, there is an urgent need for novel diagnostic approaches. To identify molecular networks and pathways which may play important roles in intervertebral disc herniation, as well as to reveal the potential features which could be useful for monitoring disease progression and prognosis, multi-omics profiling, including high-resolution liquid chromatography-mass spectrometry (LC-MS)-based metabolomics and tandem mass tag (TMT)-based proteomics was performed. Cerebrospinal fluid of nine dogs with IVDH and six healthy controls were used for the analyses, and an additional five IVDH samples were used for proteomic data validation. Furthermore, multi-omics data were integrated to decipher a complex interaction between individual omics layers, leading to an improved prediction model. Together with metabolic pathways related to amino acids and lipid metabolism and coagulation cascades, our integromics prediction model identified the key features in IVDH, namely the proteins follistatin Like 1 (FSTL1), secretogranin V (SCG5), nucleobindin 1 (NUCB1), calcitonin re-ceptor-stimulating peptide 2 precursor (CRSP2) and the metabolites N-acetyl-D-glucosamine and adenine, involved in neuropathic pain, myelination, and neurotransmission and inflammatory response, respectively. Their clinical application is to be further investigated. The utilization of a novel integrative interdisciplinary approach may provide new opportunities to apply innovative diagnostic and monitoring methods as well as improve treatment strategies and personalized care for patients with degenerative spinal disorders.
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Enfermedades de los Perros/líquido cefalorraquídeo , Desplazamiento del Disco Intervertebral/veterinaria , Animales , Estudios de Casos y Controles , Perros , Desplazamiento del Disco Intervertebral/líquido cefalorraquídeo , Metaboloma , Proteoma , ProteómicaRESUMEN
The aim of the present study was to investigate whether and to what extent the RNF4-SacII gene polymorphism influences reproduction performances in hyperprolific sow lines. The study involved 101 Landrace x Large White crossbred sows, with 461 records collected on the following reproductive traits: Total Number of piglets Born per litter (TNB), Number of piglets Born Alive per litter (NBA), Number of StillBorn piglets per litter (NSB), piglet Pre-Weaning Mortality (PWM) and Number of piglets at Weaning per litter (NW). The least square method with the GLM procedure in SAS with eight effects was used to pursue the data analysis. Study results revealed that TT homozygotes and TC heterozygotes had a significantly higher (p < .05) NW than CC homozygotes for the life-span performance in all parities and first parity analysed. In the fourth parity analysed, TNB and NBA in TC genotype were significantly higher (p < .05) as compared with TT genotype. Based on the life-span performance, significant effect (p < .05) was recorded for order of parity on TNB, NBA and NW, for farrowing season on TNB and NSB, and for lactation length on PWM. In the second parity, significant effect (p < .05) was recorded for sire of boar on NSB and for gestation length on TNB. Only in the fourth parity, significant effect (p < .05) of RNF4 gene was observed on NBA. There was significant additive effect (p < .05) of the RNF4 gene polymorphism identified on NW in all parities analysed, and significant additive and dominance effects (p < .05) on NSB in the third parity analysed. In conclusion, additional research on related production pig genotypes is necessary to elucidate the effect of RNF4 gene mutation on reproductive traits.
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Proteínas Nucleares/genética , Sus scrofa/genética , Factores de Transcripción/genética , Animales , Femenino , Lactancia/genética , Tamaño de la Camada/genética , Mortalidad , Paridad , Polimorfismo Genético , Embarazo , Reproducción/genética , Mortinato/genética , Mortinato/veterinaria , Sus scrofa/fisiologíaRESUMEN
Recently an increase has been reported in the number of HBV transmissions from anti-HBc positive blood donors that were repeatedly negative in HBsAg and nucleic acid testing using the most sensitive tests available. The aim of the study was to show the effect of anti-HBc antibody testing performed in 2006 on permanent deferral of voluntary blood donors (VBDs), and to estimate occult hepatitis B infection (OBI) rate in this population after the introduction of mandatory molecular testing in the 2013-2016 period. More than 30,000 blood donations collected during the 2005-2007 period and more than 14,000 VBDs having donated blood during the 2013-2016 period after the introduction of molecular testing from eastern Croatia were included in the study. Serologic testing was performed with HBsAg assay throughout the study period, and anti-HBc assay was only performed in 2006. As part of the confirmatory algorithm testing, all HBsAg positive and unclear results were tested with molecular tests. Anti-HBc prevalence among VBDs in 2006 was 1.5%, with a rate of 1:197, whereas HBsAg prevalence was stable from 2005 to 2007 (0.04%, 0.1% and 0.1%, respectively). The calculated OBI rate from 2013 to 2016 was 1:30,250. Ten of 161 (12.4%) VBDs had serologic anti-HBc-only pattern. Anti-HBc testing in 2006 resulted in statistically more deferrals of VBDs compared to 2005 and 2007, and to the rest of Republic of Croatia. The strategy of universal anti-HBc testing of VBDs in addition to the existing HBsAg and molecular screening could be an additional measure to prevent HBV transmission by blood and blood components.
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Donantes de Sangre , Virus de la Hepatitis B , Hepatitis B , Croacia/epidemiología , ADN Viral , Hepatitis B/diagnóstico , Hepatitis B/epidemiología , Virus de la Hepatitis B/genética , HumanosRESUMEN
Inflammatory bowel diseases are multifactorial disorders the clinical manifestation of which depends on the interaction among immune response, genetic and environmental factors. There is growing evidence that cytokines and gene polymorphisms have an important role in disease pathogenesis in various populations although molecular mechanism of their signaling and interactions is not fully understood yet. The present study aimed at exploring the effects of interleukin-6, C-reactive protein and interleukin-6 rs1800795 polymorphism on the development of Crohn's disease, ulcerative colitis and inflammatory bowel diseases overall and at determining differences between inflammatory bowel disease patients and healthy controls. A total of 132 inflammatory bowel disease patients and 71 healthy blood donors were investigated. In order to assess the clinical relevance of interleukin-6 and C-reactive protein serum concentration and interleukin-6 rs1800795 single nucleotide polymorphism in patients with Crohn's disease and ulcerative colitis, we performed a cross-sectional, case-control study. Quantitative assessment of serum interleukin-6 and C-reactive protein was performed with solid-phase, enzyme-labeled, chemiluminescent sequential immunometric and immunoturbidimetric assay, respectively. A real-time fluorescence resonance energy transfer-based method on a LightCyclerTM PCR 1.2 was used for genotyping of IL-6 rs1800795 polymorphism. Both interleukin-6 and C-reactive protein serum levels were elevated in Crohn's disease and ulcerative colitis patients. Positive correlations were observed between C-reactive protein and interleukin-6 serum concentration and ulcerative colitis activity index as measured by modified Truelove-Witt's severity index scale. C-reactive protein serum level was higher in Crohn's disease patients without intestinal resection than in Crohn's disease patients with prior intestinal resection. In ulcerative colitis patients, interleukin-6 and C-reactive protein serum levels were statistically significantly higher in CC interleukin-6 genotype in comparison to GG+GC genotype. Analysis of the promoter region of the interleukin-6 rs1800795 gene polymorphism showed no statistically significant difference in allele frequency either between inflammatory bowel disease patients and healthy controls or between the two inflammatory bowel disease phenotypes and healthy controls. Associations presented in this study give a potentially important insight into the role of interleukin-6 and C-reactive protein signaling and interleukin-6 polymorphism in the pathogenesis of Crohn's disease and ulcerative colitis disease.
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Proteína C-Reactiva , Colitis Ulcerosa , Enfermedad de Crohn , Enfermedades Inflamatorias del Intestino , Interleucina-6 , Proteína C-Reactiva/análisis , Estudios de Casos y Controles , Colitis Ulcerosa/genética , Enfermedad de Crohn/genética , Estudios Transversales , Humanos , Interleucina-6/genética , Polimorfismo GenéticoRESUMEN
The aim of this study was to investigate the influence of season, rainfall and air temperature on the reproductive efficiency in the Romanov breed of sheep in continental part of Croatia during five consecutive years (2012-2016). During this period, 5379 matings resulted in 5046 successful conceptions, i.e. lambings at eight medium-scale Romanov breed sheep farms. The conception rate was 93.81%, fecundity was 195% and average preweaning mortality until 90 days of age was 12.41%. The seasonal distribution of lambings was 47.64% for ewes that delivered in winter (n = 2422), 23.37% in spring (n = 1179), 18.82% in summer (n = 950) and 9.81% in autumn (n = 495). Sexual activity was lowest during spring and early summer when air temperatures were above average (very and extremely warm), and sexual activity peaked from August to September, especially during extremely wet and very wet seasons. Litter size was greater during winter than in other seasons (1.70 vs. 1.54) and was significantly different as compared to each of selected years of the study period. There was a statistically significant difference in the number of pregnant ewes between mating seasons. Most female Romanov lambs born during winter and early spring mated in late summer or autumn and delivered at the age of 1 year or earlier. The seasonal distribution of matings and lambing was not uniform throughout the seasons over five consecutive years. Thus, it can be assumed that air temperature and rainfall during different seasons could affect the reproductive efficiency in Romanov breed of sheep in continental part of Croatia.
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Cruzamiento , Reproducción , Animales , Croacia , Femenino , Embarazo , Estaciones del Año , Ovinos , TemperaturaRESUMEN
The aim of this study was to investigate the effects of monthly air temperature and rainfall variations on the reproductive performance and lambing distribution of the Jezersko-Solcava breed in northwestern Croatia during 7 years period from 2010 until 2016. At 11 medium-scale sheep farms, the sheep were kept on a pasture in a semi-intensive environment with access to the stables. The anomalies of rainfall observed on a monthly basis during monitoring were statistically significant. Non-parametric test showed that the percentages of lambing differ among the months during the year (P = 0.0001). By using regression analysis, it was found that the percentages of pregnant sheep were linearly associated with the sequential number of the month. The seasonal distribution of lambings were 53.85% of ewes delivered in winter (n = 1296), 21.13% in spring (n = 509), 8.91% in summer (n = 215) and 16.11% in autumn (n = 387). Sexual activity was lowest during spring and early summer (from March to May) with a peak from the end of July to October. The conception rate during year 2011 was significantly lower (89.74%), and the litter size was the lowest (1.05) than in other years of the observation. Average conception rate was 92.85% and average litter size 1.21. Despite being a seasonally polyoestrous, the distribution of matings and lambings of this breed of sheep was not uniform throughout the seasons. There were established a positive or negative correlations between the air temperature and rainfall precipitation variations with tested reproductive performances of Jezersko-Solcava breed sheep in the Northwestern part of Croatia.
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Lluvia , Reproducción , Ovinos/fisiología , Temperatura , Animales , Croacia , Femenino , EmbarazoRESUMEN
The study was performed on 16 Holstein-Friesian calves divided into two groups of eight animals each. The first group was composed of calves whose mothers did not receive selenium supplements (Se0). The second group consisted of calves whose mothers were administered intramuscular injections of a selenium and vitamin E supplement containing 0.5 of sodium selenite/ml and 50 mg of tocopherol acetate/ml in a single dose of 30 ml (Se30) ml, 10 days before the expected parturition date (10 ± 2 days). The calves were fed 2.5 L of the mother's colostrum administered by stomach tube 2 hr after birth and another 2 L 6 hr after birth. Blood from all calves was collected 7 times from external jugular vein (day 0-before colostrum administration and on the 2nd, 3rd, 4th, 7th, 14th and 21st days of life) for analyses of selenium, ceruloplasmin, transferrin, lactoferrin, immunoglobulin G (IgG) concentrations and gamma-glutamyl transferase (GGT) and lysozyme activity. Selenium concentration was significantly higher in calves whose mothers received selenium supplements than in the offspring of non-supplemented cows until 72 hr after birth (p ≤ .05). Lysozyme and GGTP activity and IgG concentration were significantly higher in the S30 group during the entire experiment (p ≤ .05). Supplementation of selenium to the mothers did not influence the ceruloplasmin, lactoferrin and transferrin levels in calves. A single injection of a selenium supplement administered to cows during late pregnancy increases selenium levels in calves and enhances passive transfer from the mother to the offspring.
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Animales Recién Nacidos/inmunología , Bovinos/fisiología , Selenio/administración & dosificación , Animales , Animales Recién Nacidos/sangre , Bovinos/inmunología , Calostro , Femenino , Inmunoglobulina G/sangre , Masculino , Muramidasa/sangre , Embarazo , Selenio/sangre , alfa-Tocoferol/administración & dosificación , gamma-GlutamiltransferasaRESUMEN
Bovine mastitis, as a significant and prevalent health problem in dairy herds, is primarily attributed to bacterial infections. Yeasts, although considered relatively rare causative agents, have also been associated with mastitis in dairy cattle. Current mastitis treatment predominantly relies on antibiotics, with limited emphasis on antifungal treatment. However, mycotic mastitis is challenging to treat, since these fungi are often resistant to antibiotics and may even utilize them for energy. In the current research, the in vivo antimicrobial activity of the essential oil-based formulation (Phyto-Bomat), as a possible alternative mastitis treatment associated with yeasts, was studied. This study involved a total of 68 animals from two dairy farms with diagnosed mastitis, and three treatment groups were established: conventional antibiotic treatment, Phyto-Bomat treatment, and the combination of both. The findings suggest significant variations in the presence of Candida samples based on the treatment administered, and the most significant difference was noted in cows treated with the combination (Phyto-Bomat and antibiotics). Yet, it is important to note that the results reveal that, regardless of the treatment type, there are statistically significant differences in the presence of Candida samples across the examined time points. These results aim to provide valuable insights into the potential of EOs as an alternative therapy in bovine mastitis, specifically targeting yeasts. Such findings could offer new strategies in the mycotic mastitis control and reducing the occurrence of secondary infections following antibiotic treatment.
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Canine babesiosis is a rapidly spreading tick-borne disease in Europe, which entails protozoan parasites invading red blood cells. Small extracellular vesicles (EVs) (< 200â¯nm) were isolated from the serum of 15 healthy and 15 by Babesia canis naturally infected dogs aimed to distinguish EV characteristics and protein profiles. There were no significant differences (P = 0.05) observed in the mean sizes and concentrations of serum EVs between the healthy and canine babesiosis groups. Despite a higher number of Canis lupus proteins detected in EVs from serum of diseased dogs, there were no statistically significant differences (P < 0.05) in the number of protein IDs between the experimental groups. We successfully identified 211 Canis lupus proteins across both experimental groups, of which 147 Canis lupus proteins were validated as being EV-associated. This data set is accessible via the ProteomeXchange PXD047647. EVs isolated from serum of B. canis infected dogs were Cd9+, Cd63+, Cd81+, and Cd82+. Furthermore, 73 Canis lupus proteins were validated as EV-associated and specific for EVs isolated from serum of B. canis-infected dogs. These were predominantly membrane and cytosolic proteins, and innate and adaptive immune system-related proteins, especially those involved in adhesion and proteoglycan mechanisms like integrins. Enrichment was also observed for proteins involved in vascular and cellular responses, including signalling pathways such as VEGF, VEGFR, and the LKB1 network. When only blood-related sites of EV expression were evaluated, the origins of EV proteins were mostly cells of immune system. These were dendritic cells, neutrophils, B cells, monocytes and platelets. In general, proteins were enriched in pathways that collectively regulate various cellular processes, including immune responses, communication, signal transduction, membrane trafficking, and apoptosis. Serum EVs and their protein cargo may have an important role in both the invasion of B. canis and the host's response to the parasitic infection, nevertheless, additional experimental research is warranted. The overall count of identified EV proteins of parasitic origin, meeting cut off criteria of two peptides and 1â¯% FDR, was relatively low.
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Babesia , Babesiosis , Enfermedades de los Perros , Vesículas Extracelulares , Proteómica , Animales , Perros , Babesia/clasificación , Babesia/aislamiento & purificación , Babesiosis/parasitología , Babesiosis/sangre , Enfermedades de los Perros/parasitología , Enfermedades de los Perros/sangre , Vesículas Extracelulares/química , Cromatografía Líquida con Espectrometría de Masas , Proteómica/métodos , Espectrometría de Masas en Tándem/veterinariaRESUMEN
Anthropogenic radiofrequency electromagnetic radiation (RF-EMR) from wireless technologies has increased dramatically. The boar semen used for artificial insemination is essential in sustaining the pig industry, and additionally it is also exposed to the effects of the RF-EMR of wireless technologies. Furthermore, there are no data on the effects of RF-EMR on semen quality, and this is the first analysis of sperm's morphometric parameters for assessing the effect of RF-EMR on the spermatozoa subpopulations of boars. This study investigated the effect of RF-EMR on in vitro exposed breeding boar semen spermatozoa motility and the proportions of spermatozoa subpopulations according to their morphometric head and tail parameters. The semen samples of 12 boars were divided into control and experimental groups. The samples in the experimental group were exposed in a gigahertz transverse electromagnetic chamber at a frequency of 2500 MHz (the frequency band used in 5G technology) and an electric field strength of 10 Vm-1 for two hours. After exposure, the spermatozoa motility was evaluated for both groups. A morphometric analysis of the semen smears was performed using SFORM software (Version 1.0; VAMS, Zagreb, Croatia). The progressive spermatozoa motility was significantly reduced in the experimental group (74.7% vs. 85.7%). PC analysis and cluster analysis revealed two spermatozoa subpopulations: S1, spermatozoa with a more regular head shape and a smaller midpiece outline, and S2, spermatozoa with a more elongated head shape and a larger midpiece outline. The experimental semen samples had a greater proportion of the S1 spermatozoa subpopulation (68.2% vs. 64.4%). The effect of RF-EMR at 2500 MHz on the in vitro exposed boar semen resulted in decreased progressive spermatozoa motility and a lower proportion of the spermatozoa subpopulation with a higher fertilizing potential.
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Vitamin K epoxide reductase (VKOR) is a key enzyme in the y-carboxylation of proteins associated with important bodily functions (coagulation, bone metabolism, etc.). This feature is particularly used in warfarin therapy, which blocks the VKOR enzyme and leads to production of dysfunctional coagulation proteins. Genetic factors, particularly vitamin K epoxide reductase complex 1 (VKORC1) gene, have greatest influence on warfarin therapy. The aim of this study was to determine the distribution of VKORC1 1173C > T and VKORC1 -1639G > A polymorphisms, which are most important for warfarin therapy. We investigated 420 unrelated healthy individuals, mostly blood donors, from region of the Eastern Croatia. Both investigated polymorphisms were in perfect linkage disequilibrium (LD) and showed identical results. 151 patients (36%) were homozygous for the wild-type (C/C and G/G), 196 (47%) were heterozygous (C/T and G/A) and 73 (17%) were homozygous for the variant allele (T/T and A/A). Number of normal alleles among individuals was 498 (59.3%), and number of variant alleles was 342 (40.7%). The data obtained are in good agreement with the results of studies in other European populations.
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Genética de Población , Polimorfismo Genético , Vitamina K Epóxido Reductasas/genética , Adulto , Anciano , Croacia , Femenino , Humanos , Masculino , Persona de Mediana Edad , Reacción en Cadena en Tiempo Real de la Polimerasa , Adulto JovenRESUMEN
Despite preventive and therapeutic measures, mastitis continues to be the most prevalent health problem in dairy herds. Considering the risks associated with antibiotic therapy, such as compromised effectiveness due to the emergence of resistant bacteria, food safety issues, and environmental impact, an increasing number of scientific studies have referred to the new therapeutic procedures that could serve as alternatives to conventional therapy. Therefore, the aim of this review was to provide insight into the currently available literature data in the investigation of non-antibiotic alternative approaches. In general, a vast number of in vitro and in vivo available data offer the comprehension of novel, effective, and safe agents with the potential to reduce the current use of antibiotics and increase animal productivity and environmental protection. Constant progress in this field could overcome treatment difficulties associated with bovine mastitis and considerable global pressure being applied on reducing antimicrobial therapy in animals.
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The aim of this study was to examine the biochemical components and the parameters of antioxidant protection in the seminal plasma (SP) of boars, as well as their relationship with semen quality parameters. Thirty-six boars were included in the study, whose ejaculates were divided into two groups: Group I (good quality semen, > 70% progressively motile sperm, < 20% spermatozoa with abnormal morphology, n = 16), and Group II (poor quality semen, < 70% progressively motile sperm, > 20% spermatozoa with abnormal morphology, n = 20). Significantly higher concentrations of ionized calcium (iCa), total cholesterol (TC), lactate-dehydrogenase (LDH) activity, as well as significantly higher values ââof antioxidant protection parameters: thiol groups (-SH), paraoxonase 1 (PON1), and total antioxidant capacity (TAC) ) were found in the good quality semen, while higher phosphorus (P) concentrations and increased alkaline-phosphatase (ALP) activity were found in the semen of poor quality. A negative correlation of total and progressive sperm motility with P and ALP was found in all examined semen samples, while a positive correlation was found with PON1 and TAC. The percentage of fast sperm cells positively correlated with iCa, chlorine (Cl), lactate, LDH and TAC, while a negative correlation was found with P, magnesium (Mg) and the enzyme creatine-kinase (CK). The percentage of immobile sperm positively correlated with P and ALP, and negatively correlated with TC, CK, PON1 and TAC. Elevated values ââof PON1 and TAC in SP and a positive correlation with sperm motility indicate the possible use of these parameters as sensitive biomarkers of boar semen quality. To the best of our knowledge there are no published data on association between PON1 activity and boar semen quality.
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Arildialquilfosfatasa , Análisis de Semen , Semen , Animales , Masculino , Antioxidantes , Semen/enzimología , Análisis de Semen/veterinaria , Motilidad Espermática , PorcinosRESUMEN
BACKGROUND: Retained placenta (RP), a quite common disorder in dairy cows, shows a high negative impact on their health status and milk production. AIM: To investigate the difference in the serum proteome between the cows with RP and the physiologic puerperium (PP). MATERIAL & METHODS: Analysis of serum samples from nine cows with RP and six with PP using high-resolution liquid chromatography-tandem mass spectrometry approach. The proteins differing in the relative abundance between the PP and RP groups were classified using the Protein Analysis Through Evolutionary Relationship tool. For the pathway enrichment analysis, the REACTOME tool, with the human genome as the background, was employed. The criterion for significance was the false discovery rate corrected P-value less than 0.05. RESULTS: In total 651 proteins were identified with altered relative abundance of ten proteins. Among them, seven had higher, and three showed lower relative abundance in RP than in the PP group. The differently abundant proteins participated in 15 pathways: six related to hemostasis, three involved in lipoprotein metabolism, and the remaining ones associated with for instance redox homeostasis, post-translational modification, and scavenging. Finally, the validation of the proteomic results showed that haptoglobin and lipopolysaccharide-binding protein levels reliably differentiated between the RP and PP groups. CONCLUSION: The pattern of serum proteome alterations in the cows with RP mirrored several interplaying mechanisms underlying the systematic response to the presence of RP, therefore representing a source to mine for predictive or prognostic biomarkers.
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Enfermedades de los Bovinos , Retención de la Placenta , Embarazo , Femenino , Humanos , Bovinos , Animales , Retención de la Placenta/veterinaria , Retención de la Placenta/metabolismo , Proteoma/análisis , Proteoma/metabolismo , Proteómica , Periodo Posparto , Lactancia/metabolismo , LecheRESUMEN
Mastitis in dairy cows is responsible for major economic losses on dairy farms worldwide as the most expensive and prevalent disease in dairy cattle. In spite of the fact that antibiotic therapy still remains the main treatment strategy for bovine mastitis, concerns about the shortcomings of this treatment approach are continuously raised. Hence, research on alternative treatments with increased effectiveness and reduced costs is needed. Therefore, we conducted a pharmacoeconomic analysis of conventional antibiotic vs. a proposed Phyto-Bomat treatment based on essential oils in bovine mastitis therapy. Treatments were compared from the farmer's perspective in the domain of costs (expressed in total, direct and indirect, cost differences) and effectiveness (expressed in daily milk yield differences). Economic calculations were based on data from a dairy farm in Serbia. The average cost of conventional antibiotic treatment was estimated at EUR 80.32 consisting of therapy costs, veterinary services and milk rejection costs at EUR 16.54, EUR 17.68 and EUR 46.10, respectively. The average cost of Phyto-Bomat treatment was estimated at EUR 76.34 with therapy costs of EUR 34.34, veterinary costs of EUR 32.00 and rejection of milk costs of only EUR 10.00. Therefore, Phyto-Bomat results in cost savings approximating EUR 4 per each mastitis episode with the highest cost reductions obtained in milk rejection costs. This estimation of Phyto-Bomat's economic benefits could be used as a starting point for the inclusion of this formulation as an alternative treatment approach with a focus on subclinical mastitis since it contributes to most of the financial losses.
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Antimicrobials have had an important impact on animal health and production performance. However, non-prudent antimicrobial use (AMU) in food producing animals is considered to contribute to the emergence of antimicrobial resistance (AMR), with a potential impact on both animal and public health. Considering the global importance of AMR, and the threats and challenges posed by mastitis and mastitis therapy in livestock production, the main objective of this study was to quantify AMU on three dairy farms in Serbia and to examine whether there is an association between AMU and the emergence of antimicrobial resistance of mastitis-associated pathogens. Antimicrobial susceptibility testing was performed by the disk diffusion method using causative agents isolated from the milk samples of 247 dairy cows. AMU data were obtained for a one-year period (May 2021 to May 2022) based on antibiotic prescriptions listed in electronic databases kept by farm veterinarians. To estimate antimicrobial drug exposure at the farm level, the veterinary drug Defined Daily Dose was calculated by multiplying the total amount of antibiotic used on the farms during the study period by the quantity of antibiotic in the administered drug and number of original drug packages used. The results on the association between the use of common antibiotics in mastitis treatment and AMR of isolated mastitis-associated pathogens confirm a pattern that could raise awareness of the importance of this aspect of good veterinary and clinical practice to combat the global threat of AMR.