Relationships among lumbar hip motion angle, perceptual awareness, and low back pain in young adults.

[Purpose] We aimed to examine the relationships among low back pain, lumbar-hip motion angle, and lumbar perceptual awareness in young adults to improve the treatment of low back pain. [Participants and Methods] Data were collected from 36 university students with low back pain. The items included for evaluation were the low back pain intensity (Numeric Rating Scale), disability due to low back pain (Oswestry Low Back Pain Disability Index), lumbar spine and hip motion angles in test movements, and perceptual awareness (Fremantle Back Awareness Questionnaire). The test movements employed included trunk forward bending, trunk back bending, and prone hip extension. The motion angles of the lumbar spine and hip joints were measured using a wearable sensor. [Results] The Numeric Rating Scale was not correlated with the lumbar hip motion angle and perceptual awareness. The Oswestry Low Back Pain Disability Index was correlated with lumbar hip motion angles, at the beginning of trunk forward bending and at maximum trunk backward bending, and with perceptual awareness. [Conclusion] There are relationships among disabilities due to low back pain, lumbar hip motion angles, and perceptual awareness in each test movement; however, they vary depending on the type and angle of the test movement conducted.

Optical system to display mid-air images on a glossy plane and remove ground images.

Mid-air images are formed in the air by the reflection and refraction of light emitted by a light source, which allows the user to view the floating image in real space without wearing special equipment. However, conventional mid-air image optical systems have some weaknesses, such as the need to suitably adjust the height of the viewpoint position depending on its optical arrangement. We propose an optical design that can be installed simply by placing it on a glossy plane, on which an upright mid-air image can be displayed and which is smaller than the existing system.

Left-Right Difference in Brain Pharmacokinetics Following Nasal Administration Via One-Site Nostrils.

The olfactory and trigeminal pathways are direct delivery pathways between the nose and brain. To determine the effect of direct delivery on drug distribution in the brain, two model drugs with different physical properties, antipyrine (ANP), with high membrane permeability, and ranitidine (RNT), with low membrane permeability, were selected. For ANP, direct delivery from the nose to the brain was observed only in the olfactory bulb beside the nasal cavity, with a direct transport percentage (DTP) of approximately 45 %, whereas in the frontal and occipital brains, the contribution from the systemic circulation to the brain was observed as the primary route of brain distribution. No significant variations were observed in the pharmacokinetics of ANP in the left and right brain, whereas RNT was distributed in all brain regions with a DTP of > 95 %. The closer the brain region is to the nasal cavity, the higher the DTP. Furthermore, the left brain, the same nostril site (left nostril) of administration, had a larger level of drug delivery than the right brain. These findings imply that the influence of the administered nostril site differs based on the physicochemical properties and amount of the drug.

Developmental changes in excitation-contraction mechanisms of the mouse ventricular myocardium as revealed by functional and confocal imaging analyses.

Developmental changes in excitation-contraction mechanisms were examined in the ventricular myocardium from fetal, neonatal, and 1-, 2-, and 4-week-old mice. In isolated tissue, the negative inotropic effect of nifedipine decreased, while that of ryanodine increased with age. Action potential duration decreased with age, especially during the late fetal period. In ventricular cardiomyocytes, fluorescence imaging revealed that the sarcoplasmic reticulum increases progressively during pre- and postnatal development. t-Tubules were absent in the fetus and neonate, were observed only in the subsarcolemmal region at 1 week after birth, and were present throughout the cytoplasm at 2 and 4 weeks after birth. The amplitude of Ca(2+) transients, as well as its ryanodine-sensitive component, increased with age. In the neonate and 1-week-old mice, Ca(2+) at the cell center showed slower rise than the subsarcolemmal region, but in 2- and 4-week-old mice, Ca(2+) increased simultaneously across the entire width of the cell. These results suggest that in the mouse ventricular myocardium, the shortening of the action potential during the late fetal period and the development of t-tubule-sarcoplasmic reticulum coupling during the second postnatal week largely contribute to the developmental increase in the dependence of contraction on sarcoplasmic reticulum function.

Relationship between lumbar spine motor control ability and perceptual awareness during prone hip extension movement in people with low back pain.

PURPOSE: The purpose of this study was to clarify the differences in lumbar spine and hip joint motor control ability (MCA) in prone hip extension (PHE) between individuals with and without low back pain (LBP). It also aimed to determine the relationship between lumbar spine and hip joint MCA and lumbar perceptual awareness in individuals with LBP. METHODS: In total, 78 university students (20 with LBP and 58 without) were included in the study. The MCA of the lumbar spine and hip joint in PHE and perceptual awareness were evaluated. The MCA of the lumbar spine and hip joint was measured using a wearable sensor. Subsequently, a comparison of the MCA of the lumbar spine and hip joints of the participants and the relationship between MCA and lumbar perceptual awareness were examined. RESULTS: The MCA of the LBP group was higher than that of the non-LBP group in motion on the sagittal plane. In addition, perceptual awareness was negatively correlated with MCA in the sagittal plane in the lumbar spine. CONCLUSION: People with LBP had higher lumbar spine and hip joint MCA than those without LBP. Perceptual awareness was associated with lumbar spine and hip joint MCA in people with LBP. J. Med. Invest. 69 : 38-44, February, 2022.

Measuring Lumbar Motion Angle With a Small Accelerometer: A Reliability Study.

Objective: The purpose of this study was to investigate the reliability of measuring the lumbar motion angle using a small accelerometer for movement tests in the sagittal plane, and to determine the number of measurements required to obtain reliable data. Methods: In total, 102 healthy adults (56% women, 44% men; 20.1 ± 1.1 years of age) participated in this study. The intra- and interexaminer reliability of lumbar motion angle measurements during forward and backward trunk bending and prone hip extension were investigated. Lumbar motion angles were measured with small accelerometers placed at the thoracolumbar transition, lumbosacral transition, and thigh, during motion and at the final position. Intraclass correlation coefficients and Bland-Altman analysis were used for statistical analysis. Results: The intraclass correlation coefficients ranged from 0.6 to 0.8 in intraexaminer analyses and from 0.5 to 0.7 in interexaminer analyses. Intraexaminer systematic biases were found only in the maximum angle of trunk forward bending. Interexaminer biases were found in all angles of trunk forward bending. Conclusion: In the measurement of lumbar motion angle in the sagittal plane using a small accelerometer, it was necessary to repeat measurements 2 to 4 times, because a single measurement was not sufficiently reliable either within or between examiners.

Protection of 6-OHDA neurotoxicity by PGF2α through FP-ERK-Nrf2 signaling in SH-SY5Y cells.

6-Hydroxydopamine (6-OHDA) is a neurotoxin that destroy dopaminergic neurons and widely used to establish animal models of Parkinson's disease. Prostaglandins (PGs) are involved in various cellular processes, including the damage and repair of neuronal cells. However, the function of PGF2α in neuronal cells remains unclear. In this study, we investigated the effects of PGF2α against 6-OHDA-mediated toxicity in human neuroblastoma SH-SY5Y cells and elucidated its underlying molecular mechanism. When the cells were treated with 6-OHDA (50 µM) for 6 h, the expression levels of PGF2α synthetic enzymes; cyclooxygenase-2 and aldo-keto reductase 1C3 as PGF2α synthase were enhanced in an incubation-time-dependent manner. In addition, the production of PGF2α was increased in 6-OHDA-treated cells. Fluprostenol, a PGF2α receptor (FP) agonist (500 nM), suppressed 6-OHDA-induced cell death by decreasing the production of reactive oxygen species (ROS) and increasing the expression of the anti-oxidant genes. These fluprostenol-mediated effects were inhibited by co-treatment with AL8810, an FP receptor antagonist (1 µM) or transfection with FP siRNA (20 nM). Moreover, 6-OHDA-induced phosphorylation of extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinase family, was inhibited by co-incubation with AL8810. Furthermore, fluprostenol itself enhanced ERK phosphorylation and further elevated the 6-OHDA-induced phosphorylation of ERK. In addition, 6-OHDA induced nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), activating anti-oxidant gene expression, was repressed by co-culturing with AL8810. These results indicate that PGF2α suppressed 6-OHDA-induced neuronal cell death by enhancing anti-oxidant gene expression via the FP receptor-ERK-Nrf2 signaling. Thus, FP receptor is a potential target for inhibition of ROS-mediated neuronal cell death.

An analysis of current source density profiles activated by local stimulation in the mouse auditory cortex in vitro.

To examine local network properties of the mouse auditory cortex in vitro, we recorded extracellular spatiotemporal laminar profiles driven by short electric local stimulation on a planar multielectrode array substrate. The recorded local field potentials were subsequently evaluated using current source density (CSD) analysis to identify sources and sinks. Current sinks are thought to be an indicator of net synaptic current in the small volume of cortex surrounding the recording site. Thus, CSD analysis combined with multielectrode arrays enabled us to compare mean synaptic activity in response to small current stimuli on a layer-by-layer basis. We also used senescence-accelerated mice (SAM), some strains of which show earlier onset of age-related hearing loss, to examine the characteristic spatiotemporal CSD profiles stimulated by electrodes in specific cortical layers. Thus, the CSD patterns were classified into several clusters based on stimulation sites in the cortical layers. We also found some differences in CSD patterns between the two SAM strains in terms of aging according to principle component analysis with dimension reduction. For simultaneous two-site stimulation, we modeled the obtained CSD profiles as a linear superposition of the CSD profiles to individual single-site stimulation. The model analysis indicated the nonlinearity of spatiotemporal integration over stimulus-driven activity in a layer-specific manner. Finally, on the basis of these results, we discuss the auditory cortex local network properties and the effects of aging on these mouse strains.

Salicylate-Induced Suppression of Electrically Driven Activity in Brain Slices from the Auditory Cortex of Aging Mice.

The prevalence of tinnitus is known to increase with age. The age-dependent mechanisms of tinnitus may have important implications for the development of new therapeutic treatments. High doses of salicylate can be used experimentally to induce transient tinnitus and hearing loss. Although accumulating evidence indicates that salicylate induces tinnitus by directly targeting neurons in the peripheral and central auditory systems, the precise effect of salicylate on neural networks in the auditory cortex (AC) is unknown. Here, we examined salicylate-induced changes in stimulus-driven laminar responses of AC slices with salicylate superfusion in young and aged senescence-accelerated-prone (SAMP) and -resistant (SAMR) mice. Of the two strains, SAMP1 is known to be a more suitable model of presbycusis. We recorded stimulus-driven laminar local field potential (LFP) responses at multi sites in AC slice preparations. We found that for all AC slices in the two strains, salicylate always reduced stimulus-driven LFP responses in all layers. However, for the amplitudes of the LFP responses, the two senescence-accelerated mice (SAM) strains showed different laminar properties between the pre- and post-salicylate conditions, reflecting strain-related differences in local circuits. As for the relationships between auditory brainstem response (ABR) thresholds and the LFP amplitude ratios in the pre- vs. post-salicylate condition, we found negative correlations in layers 2/3 and 4 for both older strains, and in layer 5 (L5) in older SAMR1. In contrast, the GABAergic agonist muscimol (MSC) led to positive correlations between ABR thresholds and LFP amplitude ratios in the pre- vs. post-MSC condition in younger SAM mice from both strains. Further, in younger mice, salicylate decreased the firing rate in AC L4 pyramidal neurons. Thus, salicylate can directly reduce neural excitability of L4 pyramidal neurons and thereby influence AC neural circuit activity. That we observed age-dependent effects of salicylate and varied GABAergic sensitivity in the AC among mouse strains with hearing loss implies that potential therapeutic mechanisms for tinnitus may operate differently in young vs. aged subjects. Therefore, scientists developing new therapeutic modalities for tinnitus treatment should consider using both aged and young animals.