RESUMEN
We demonstrated that beta-glucosidase and beta-galactosidase can be trapped inside erythrocytes by rapid hemolysis of the cell in the presence of these enzymes. Enzyme enters only during hemolysis, and optimum uptake occurs within 60 sec. There is no loss in cell number after hemolysis-induced enzyme uptake, and the ghosts have only a slightly increased mean cell volume. Smaller proteins enter more readily than larger proteins, although enzymes with a molecular weight of at least 180,000 can be readily entrapped by erythrocytes. This finding may provide a useful approach to the problem of enzyme replacement in certain diseases, including Gaucher's disease.
Asunto(s)
Enzimas/administración & dosificación , Eritrocitos/enzimología , Animales , Catalasa/metabolismo , Cerebrósidos , Grupo Citocromo c/metabolismo , Terapia Enzimática , Escherichia coli/enzimología , Galactosidasas/metabolismo , Enfermedad de Gaucher/tratamiento farmacológico , Glucosa , Glucosidasas/metabolismo , Glicósido Hidrolasas/administración & dosificación , Glicósido Hidrolasas/uso terapéutico , Hemólisis , Humanos , Soluciones Hipotónicas , Inmunoglobulinas/metabolismo , Riñón/enzimología , Errores Innatos del Metabolismo/tratamiento farmacológico , Peso Molecular , Fagocitosis , Ratas , Albúmina Sérica/metabolismo , Cloruro de Sodio , Isótopos de AzufreRESUMEN
Intramural gastrointestinal hemorrhage should be suspected in patients taking anticoagulants who develop acute abdominal symptoms with clotting parameters out of the therapeutic range. Likewise, patients with inherent bleeding disorders may present with intramural hemorrhage. Characteristic features are seen on radiological examination; ultrasonography or computed tomography may be valuable noninvasive diagnostic approaches. The diagnosis may be supported by abdominal paracentesis. Surgical intervention is not necessary as conservative treatment, with replacement of clotting factors leading to rapid recovery.