RESUMEN
11 beta-Hydroxydehydroepiandrosterone has been isolated from the urine of a 14-yr-old girl with a virilizing adrenal adenoma. Its excretion was estimated to be 0.4 mg/24 h by gas chromatography and the compound was further identified by mass spectrometry. When [7-3H]dehydroepiandrosterone was incubated with mitochondria prepared from the adenoma, approximately 10% was converted to 11 beta-hydroxydehydroepiandrosterone. The identity of the radioactive 11 beta-hydroxydehydroepiandrosterone was verified by reversed isotopic dilution, its conversion to 11 beta-hydroxyandrostenedione, and its mobility in several chromatographic systems. This is the first demonstration of an 11 beta-hydroxylase from a human source having an affinity for dehydroepiandrosterone.
Asunto(s)
Adenoma/metabolismo , Neoplasias de las Glándulas Suprarrenales/metabolismo , Deshidroepiandrosterona/análogos & derivados , Deshidroepiandrosterona/metabolismo , Mitocondrias/metabolismo , Adenoma/cirugía , Adolescente , Neoplasias de las Glándulas Suprarrenales/cirugía , Deshidroepiandrosterona/orina , Femenino , Humanos , Virilismo/etiologíaAsunto(s)
Glándulas Suprarrenales/metabolismo , Hormona Adrenocorticotrópica/farmacología , Compuestos de Anilina/farmacología , Anticonvulsivantes/farmacología , Colesterol/metabolismo , Piridonas/farmacología , Acetatos/metabolismo , Glándulas Suprarrenales/efectos de los fármacos , Aminoglutetimida/farmacología , Animales , Isótopos de Carbono , Cromatografía , Cromatografía en Capa Delgada , Depresión Química , Ésteres/metabolismo , Femenino , Técnicas In Vitro , Ovinos , Estadística como Asunto , Estimulación QuímicaRESUMEN
Immature female rats (21 to 23 days old, 35 to 45 g) were injected subcutaneously with 2-5 i. u. HCG 18 hr before autopsy. Ovaries and uteri were removed; wet weight, dry weight and uterine protein content were determined. Ovarian and uterine weights, ovarian blood volume and uterine protein content were increased after HCG treatment. When immature female rats were pretreated with indomethacin, flufenamic acid or aspirin, the ovarian effects of HCG were inhibited: only slight increases in ovarian weight and blood volume were observed. Indomethacin attenuated the increases in uterine weight, and protein content, but neither flufenamic acid nor aspirin were effective in inhibiting these responses. The possible role of prostaglandins and of oestrogen as mediators of these responses is discussed.