Small-Intensity Rainfall Triggers Greater Contamination of Rubber-Derived Chemicals in Road Stormwater Runoff from Various Functional Areas in Megalopolis Cities.

Rubber-derived chemicals (RDCs) originating from tire and road wear particles are transported into road stormwater runoff, potentially threatening organisms in receiving watersheds. However, there is a lack of knowledge on time variation of novel RDCs in runoff, limiting initial rainwater treatment and subsequent rainwater resource utilization. In this study, we investigated the levels and time-concentration profiles of 35 target RDCs in road stormwater runoff from eight functional areas in the Greater Bay Area, South China. The results showed that the total concentrations of RDCs were the highest on the expressway compared with other seven functional areas. N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD), 6PPD-quinone, benzothiazole, and 1,3-diphenylguanidine were the top four highlighted RDCs (ND-228840 ng/L). Seasonal and spatial differences revealed higher RDC concentrations in the dry season as well as in less-developed regions. A lag effect of reaching RDC peak concentrations in road stormwater runoff was revealed, with a lag time of 10-90 min on expressways. Small-intensity rainfall triggers greater contamination of rubber-derived chemicals in road stormwater runoff. Environmental risk assessment indicated that 35% of the RDCs posed a high risk, especially PPD-quinones (risk quotient up to 2663). Our findings contribute to a better understanding of managing road stormwater runoff for RDC pollution.

The association between type 2 diabetes mellitus/prediabetes status and femoral neck bone mineral density in old adults.

BACKGROUND: The prevalence of both type 2 diabetes mellitus (T2DM) and osteoporosis has been increasing among older individuals, with these two health conditions often coexisting. Our aim in this study was to evaluate the association between T2DM status and bone mineral density (BMD) of the femoral neck among older adults in the United States. METHODS: This was a retrospective analysis of the data from 5695 adults, 60-80 years of age. The data were obtained from the National Health and Nutrition Examination Survey, for the following years: 2005-2006, 2007-2008, 2009-2010, 2013-2014, and 2017-2018. Weighted multivariable regression analyses, with subgroup analyses as appropriate, were performed to identify an association between T2DM/prediabetes status and femoral BMD and mediating factors. RESULTS: There was a significant positive association between T2DM/prediabetes status and femoral neck BMD among older women, but not men, after adjusting for body mass index (BMI). On subgroup analysis, stratified by BMI, the significant positive association was retained for T2DM women with a BMI of 25-29.9 kg/m2 (ß, 0.030; 95% CI, 0.007-0.052) or ≥30 kg/m2 (ß, 0.029; 95% CI, 0.007-0.05), and for prediabetes women with a BMI of 25-29.9 kg/m2 (ß, 0.016; 95% CI, 0.001-0.030). CONCLUSIONS: The association between a positive T2DM/prediabetes status and femoral neck BMD differed by sex among older individuals, with the association being further modulated by BMI. For women with a BMI of 25-29.9 kg/m2 or ≥30 kg/m2, T2DM was associated with a significantly higher femoral neck BMD, compared to the non-diabetes group.

Toxicity of urban stormwater on Chlorella pyrenoidosa: Implications for reuse safety.

Urban stormwater is an alternative water source used to mitigate water resource shortages, and ensuring the safety of stormwater reuse is essential. An in-depth understanding of both individual pollutant concentrations/loads in stormwater and holistic stormwater quality can be used to comprehensively evaluate how safely stormwater can be reused. The toxicity test takes all pollutants present in water samples into account, and the results reflect the integrated effect of these pollutants. In this study, the influence of urban stormwater sourced from different land uses on microalgae (Chlorella pyrenoidosa) and the possible toxicity mechanisms were investigated. The results showed that urban stormwater, particularly residential road stormwater, significantly inhibited microalgal growth. The chlorophyll contents of microalgae exposed to residential road stormwater were relatively lower, while the corresponding values were relatively higher for microalgae exposed to grassland road stormwater. Additionally, the antioxidant-related metabolism of microalgae could be dysregulated due to exposure to urban stormwater. A possible toxicity mechanism is that urban stormwater influences metabolic pathways related to chlorophyll synthesis and further hinders photosynthesis and hence microalgal growth. To resist oxidative stress and maintain regular microalgal cell activities, the ribosome metabolism pathway was upregulated. The research results contribute to elucidating the toxicity effects of urban stormwater and hence provide useful insight for ensuring the safety of stormwater reuse. It is also worth noting that the study outcomes can only represent the influence of land use on stormwater toxicity, while the impacts of other factors (particularly rainfall-runoff characteristics) have not been considered. Therefore, the consideration of all influential factors of stormwater is strongly recommended to generate more robust results in the future and provide more effective guidance for real practices related to stormwater reuse safety.

METTL3 Promotes Nucleus Pulposus Cell Senescence in Intervertebral Disc Degeneration by Regulating TLR2 m6A Methylation and Gut Microbiota.

BACKGROUND: Nucleus pulposus cell (NPC) senescence in intervertebral disc (IVD) tissue is the major pathological cause of intervertebral disc degeneration (IDD). N6-methyladenosine (m6A) methylation and gut microbiota play important roles in the progression of IDD. This study investigated whether methyltransferase-like 3 (METTL3) regulates TLR2 m6A modification and gut microbiota to influence NPC senescence. METHODS: An IDD rat model was established by lumbar IVD puncture and NPCs were challenged with IL-1ß to mimic IVD injury. IDD rats and IL-1ß-exposed NPCs were treated with METTL3-interfering lentivirus and the TLR2 agonist Pam3CSK4. Compositional changes in the rat gut microbiota were analyzed and fecal microbiota transplantation procedures were used. NPC senescence, cell cycle, and the expression of senescence-associated secretory phenotype (SASP) factors were assessed. The m6A enrichment of TLR2 and the binding of IGF2BP1 to TLR2 mRNA were examined. RESULTS: METTL3 and TLR2 were highly expressed in IDD rats. METTL3 silencing attenuated senescent phenotypes and reduced secretion of SASP factors. Pam3CSK4 reversed the beneficial effects of METTL3 silencing on NPC senescence and IVD injury. METTL3 stabilized TLR2 mRNA in an IGF2BP1-dependent manner. METTL3 silencing restored specific gut microbiota levels in IDD rats, which was further reversed by administration of Pam3CSK4. Fecal microbiota from METTL3 silenced IDD rats altered the pathological phenotypes of IDD rats. CONCLUSIONS: These results demonstrate the beneficial effects of METTL3 silencing on NPC senescence and amelioration of IVD injury, involving modulation of TLR2 m6A modification and gut microbiota. These findings support METTL3 silencing as a potential therapeutic target for IDD.

Senescent-like macrophages mediate angiogenesis for endplate sclerosis via IL-10 secretion in male mice.

Endplate sclerosis is a notable aspect of spine degeneration or aging, but the mechanisms remain unclear. Here, we report that senescent macrophages accumulate in the sclerotic endplates of lumbar spine instability (LSI) or aging male mouse model. Specifically, knockout of cdkn2a (p16) in macrophages abrogates LSI or aging-induced angiogenesis and sclerosis in the endplates. Furthermore, both in vivo and in vitro studies indicate that IL-10 is the primary elevated cytokine of senescence-related secretory phenotype (SASP). Mechanistically, IL-10 increases pSTAT3 in endothelial cells, leading to pSTAT3 directly binding to the promoters of Vegfa, Mmp2, and Pdgfb to encourage their production, resulting in angiogenesis. This study provides information on understanding the link between immune senescence and endplate sclerosis, which might be useful for therapeutic approaches.

Occurrence and risk associated with urban road-deposited microplastics.

An in-depth understanding of urban road-deposited MPs is important for the accurate prediction of the risk posed by MPs in different exposure scenarios. This study provides new insights into the intrinsic/extrinsic factors in terms of the variability of concentration and species in urban road-deposited MPs. The study results confirmed that a considerable abundance of road-deposited MPs can be identified with the average concentration ranging from 0.33 to 3.64 g m-2. Land use types and sediment particle size are the important factors that contribute to MPs abundance. The majority of detected MPs including polyethylene (PE), polypropylene (PP), polystyrene (PS) and polyethylene terephthalate (PET) are mainly derived from anthropogenic activities in commercial and residential land uses while rubber MP particles in urban road surfaces are mainly derived from tyre wear. The significant correlation (p < 0.05) between MPs and fine dust particles (< 150 µm) indicated the high affinity of small dust particles for MPs. The risk scores from MPs varied greatly from 10 to 11,000 among the study sites, which indicated the significant spatial variation of potential environmental risks posed by road-deposited MPs. The hotspots of risks posed by MPs were in areas with a high fraction of industrial, commercial and residential land uses. Specifically, the highest risk from MPs was found in mixed industrial and residential areas.