RESUMEN
A GIS-based risk index model was developed to quantify EEEV transmission risk to horses in the State of Florida. EEEV is a highly pathogenic arbovirus that is endemic along the east coast of the United States, and it is generally fatal to both horses and humans. The model evaluates EEEV transmission risk at individual raster cells in map on a continuous scale of 0 to 1. The risk index is derived based on local habitat features and the composition and configuration of surrounding land cover types associated with EEEV transmission. The model was verified and validated using the locations of documented horse cases of EEEV. These results of the verification and validation indicate that the model is able to predict locations of EEEV transmission to horses broadly across the state. The model is relatively robust to regional variation in EEEV transmission and habitat conditions in Florida, and it accurately predicted nearly all verification and validation cases in the Panhandle, North, and Central regions of the state. The model performed less accurately in the South, where relatively few cases are documented. Despite these differences, the model provides a useful way to assess EEEV risk both from a regional perspective and at more localized scales. The resulting predictive maps are designed to guide EEEV surveillance and prevention efforts by county mosquito control districts.
RESUMEN
Sequencing of dengue virus type 1 (DENV-1) strains isolated in Key West/Monroe County, Florida, indicate endemic transmission for >2 years of a distinct and predominant sublineage of the American-African genotype. DENV-1 strains isolated elsewhere in Florida grouped within a separate Central American lineage. Findings indicate endemic transmission of DENV into the continental United States.
Asunto(s)
Anticuerpos Antivirales/sangre , Virus del Dengue/genética , Dengue/epidemiología , Enfermedades Endémicas , Proteínas Virales/genética , Aedes/virología , Animales , Dengue/sangre , Dengue/virología , Virus del Dengue/clasificación , Virus del Dengue/aislamiento & purificación , Florida/epidemiología , Genotipo , Humanos , Incidencia , Insectos Vectores/virología , Filogenia , Estudios Retrospectivos , Análisis de Secuencia de ADN , Proteínas Virales/clasificaciónRESUMEN
Eastern Equine Encephalitis virus (EEEV; family Togaviridae, genus Alphavirus) a highly pathogenic mosquito-borne virus is endemic to eastern North America. The ecology of EEEV in Florida differs from that in other parts of the United States; EEEV in the northeastern United States is historically associated with freshwater wetlands. No formal test of habitat associations of EEEV in Florida has been reported. Geographical Information Sciences (GIS) was used in conjunction with sentinel chicken EEEV seroconversion rate data as a means to examine landscape features associated with EEEV transmission in Walton County, FL. Sentinel sites were categorized as enzootic, periodically enzootic, and negative based on the number of chicken seroconversions to EEEV from 2005 to 2009. EEEV transmission was then categorized by land cover usage using Arc GIS 9.3. The land classification data were analyzed using the Kruskal-Wallis test for each land use class to determine which habitats may be associated with virus transmission as measured by sentinel chicken seroconversion rates. The habitat class found to be most significantly associated with EEEV transmission was tree plantations. The ecological factor most commonly associated with reduced levels of EEEV transmission was vegetated nonforest wetlands. Culiseta melanura (Coquillett), the species generally considered to be the major enzootic EEEV vector, was relatively evenly distributed across all habitat classes, while Aedes vexans (Meigen) and Anopheles crucians Weidemann were most commonly associated with tree plantation habitats.
Asunto(s)
Ecosistema , Encefalomielitis Equina Oriental/transmisión , Animales , Pollos , Culicidae , Florida , Densidad de PoblaciónRESUMEN
BACKGROUND: Eastern Equine Encephalitis virus (EEEV) is an alphavirus with high pathogenicity in both humans and horses. Florida continues to have the highest occurrence of human cases in the USA, with four fatalities recorded in 2010. Unlike other states, Florida supports year-round EEEV transmission. This research uses GIS to examine spatial patterns of documented horse cases during 2005-2010 in order to understand the relationships between habitat and transmission intensity of EEEV in Florida. METHODS: Cumulative incidence rates of EEE in horses were calculated for each county. Two cluster analyses were performed using density-based spatial clustering of applications with noise (DBSCAN). The first analysis was based on regional clustering while the second focused on local clustering. Ecological associations of EEEV were examined using compositional analysis and Euclidean distance analysis to determine if the proportion or proximity of certain habitats played a role in transmission. RESULTS: The DBSCAN algorithm identified five distinct regional spatial clusters that contained 360 of the 438 horse cases. The local clustering resulted in 18 separate clusters containing 105 of the 438 cases. Both the compositional analysis and Euclidean distance analysis indicated that the top five habitats positively associated with horse cases were rural residential areas, crop and pastureland, upland hardwood forests, vegetated non-forested wetlands, and tree plantations. CONCLUSIONS: This study demonstrates that in Florida tree plantations are a focus for epizootic transmission of EEEV. It appears both the abundance and proximity of tree plantations are factors associated with increased risk of EEE in horses and therefore humans. This association helps to explain why there is are spatially distinct differences in the amount of EEE horse cases across Florida.
Asunto(s)
Encefalomielitis Equina Oriental/veterinaria , Mapeo Geográfico , Enfermedades de los Caballos/epidemiología , Animales , Análisis por Conglomerados , Encefalomielitis Equina Oriental/epidemiología , Florida/epidemiología , Caballos , Humanos , IncidenciaRESUMEN
Eastern equine encephalitis virus (EEEV; family Togaviridae, genus Alphavirus) epizootics are infrequent, but they can lead to high mortality in infected horses and humans. Despite the importance of EEEV to human and animal health, little is known about how the virus overwinters and reinitiates transmission each spring, particularly in temperate regions where infected adult mosquitoes are unlikely to survive through the winter. One hypothesis to explain the mechanism by which this virus persists from year to year is the spring recrudescence of latent virus in avian reservoir hosts. In this study, we tested the recrudescence hypothesis with gray catbirds (Dumatella carolinensis) captured in northern Ohio (July-August 2007). Birds were experimentally infected with EEEV on 1 October 2007. In January 2008, they were then exposed to exogenous testosterone and/or extended photoperiod to initiate reactivation of latent EEEV infection. All birds became viremic with EEEV, with mean viremia of 6.0 log10 plaque-forming units/ml serum occurring at 1 d postinoculation. One male in the testosterone, long-day treatment group had EEEV viral RNA in a cloacal swab collected on 18 January 2008. Otherwise, no other catbirds exhibited reactivated infections in cloacal swabs or blood. Antibody titers fluctuated over the course of the study, with lowest titers observed in January 2008, which corresponded with the lowest mean weight of the birds. No EEEV viral RNA was detected in the blood, kidney, spleen, brain, liver, and lower intestine upon necropsy at 19 wk postinfection.
Asunto(s)
Culicidae/virología , Virus de la Encefalitis Equina del Este/fisiología , Pájaros Cantores/virología , Animales , Enfermedades de las Aves/virología , Clima Frío , Virus de la Encefalitis Equina del Este/genética , Virus de la Encefalitis Equina del Este/crecimiento & desarrollo , Virus de la Encefalitis Equina del Este/aislamiento & purificación , Encefalomielitis Equina Oriental/epidemiología , Femenino , Masculino , Mosquiteros , Ohio/epidemiología , Estaciones del Año , Pájaros Cantores/sangre , Testosterona/sangre , Viremia/virologíaRESUMEN
We isolated and characterized St. Louis encephalitis virus (SLEV) from cloacal swabs of naturally exposed adult sentinel chickens in 2006. Phylogenetic analysis of SLEV strains isolated in Florida indicated that Brazilian SLEV circulated in 1972 and 2006; lineages were VA and VB.
Asunto(s)
Pollos/virología , Virus de la Encefalitis de San Luis/genética , Virus de la Encefalitis de San Luis/aislamiento & purificación , Animales , Enfermedades Transmisibles Emergentes/transmisión , Reservorios de Enfermedades/virología , Virus de la Encefalitis de San Luis/clasificación , Encefalitis de San Luis/epidemiología , Encefalitis de San Luis/transmisión , Florida/epidemiología , Genotipo , Humanos , Filogenia , Vigilancia de GuardiaRESUMEN
In this study, the upregulated expression level of vascular endothelial growth factor (VEGF) in a pulmonary endothelial cell line (HPMEC-ST1.6R) infected with dengue virus serotypes 1, 2, 3, and 4 (DENV-1, -2, -3 and -4), was investigated. This cell line exhibits the major constitutive and inducible endothelial cell characteristics, as well as angiogenic response. Infection by all four DENV serotypes was confirmed by an observed cytopathic effect (CPE), as well as RT-PCR (reverse-transcription polymerase chain reaction) assays. As we had previously reported, the DENV-infected HPMEC-ST1.6R cells exhibited an elongated cytoplasmic morphology, possibly representing a response to VEGF and activation of angiogenesis. In this study, increase in VEGF expression level at designated time points of 0, 8, 24, 96 and 192 hours post-infection was investigated, using a microbead-based Bio-Plex immunoassay. Increased level of VEGF expression in infected-HPMEC-ST1.6R was detected at 8 hours post-infection. Interestingly, VEGF expression level began to decrease up to 96 hours post-infection, after which an upsurge of increased VEGF expression was detected at 192 hours post-infection. This profile of VEGF upregulated expression pattern associated with DENV infection appeared to be consistent among all four DENV-serotypes, and was not observed in mock-infected cells. In this study, the expression level of VEGF, a well-established vascular permeabilizing agent was shown to be elevated in a time-dependent manner, and exhibited a unique dual-response profile, in a DENV-infected endothelial cell. The experimental observation described here provided additional insights into potential mechanism for VEGF-mediated vascular leakage associated with DENV, and support the idea that there are potential applications of anti-VEGF therapeutic interventions for prevention of severe DENV infections.
Asunto(s)
Virus del Dengue/fisiología , Células Endoteliales/virología , Perfilación de la Expresión Génica , Regulación hacia Arriba , Factor A de Crecimiento Endotelial Vascular/biosíntesis , Humanos , Factores de TiempoRESUMEN
Eastern equine encephalitis virus (EEEV) infection results in high mortality in infected horses and humans. Florida has been identified as an important source of EEEV epidemics to other states in the United States. In this study, we further characterized the epidemiological and evolutionary dynamics of EEEV in Florida. Epidemiological analysis of sentinel chicken seroconversion rates to EEEV infections during 2005-2016 suggested significant seasonality of EEEV activity in Florida. We observed significant annual activity of EEEV in the North and North Central regions, with little significant seasonality in the Panhandle region. Phylogenetic analysis of complete EEEV genome sequences from different host sources and regions in Florida during 1986-2014 revealed extensive genetic diversity and spatial dispersal of the virus within Florida and relatively more clustering of the viruses in the Panhandle region. We found no significant association between EEEV genetic variation and host source. Overall, our study revealed a complex epidemiological dynamic of EEEV within Florida, implicating the Panhandle region as a possible source of the virus with sustained year-round transmission. These findings will help in implementing targeted control measures that can have the most impact in reducing or eliminating EEEV and other mosquito-borne viral infections within Florida and in the rest of the United States.
Asunto(s)
Pollos/virología , Encefalomielitis Equina Oriental/epidemiología , Monitoreo Epidemiológico/veterinaria , Variación Genética , Estaciones del Año , Animales , Anticuerpos Antivirales/sangre , Virus de la Encefalitis Equina del Este/genética , Encefalomielitis Equina Oriental/sangre , Florida/epidemiología , Genoma Viral , Geografía , Filogenia , Salud Pública , SeroconversiónRESUMEN
In this study, the ability of dengue virus serotypes 2 (DENV-2) and 3 (DENV-3) to infect and induce increased production of proinflammatory cytokines in a pulmonary endothelial cell line (HPMEC-ST1.6R) was investigated. This cell line exhibits the major constitutive and inducible endothelial cell characteristics, as well as angiogenic response. DENV-2 and DENV-3 infection was confirmed by an observed cytopathic effect (CPE), as well as RT-PCR and immunofluorescence assays. Increases in Th-1 and Th-2 cytokines IL-4, IL-8, IL-6, IL-10, GM-CSF, INF-gamma, and tumor necrosis factor (TNF-alpha) within DENV-2- and DENV-3-infected cells were demonstrated using a microbead-based Bio-plex assay. Proinflammatory cytokine increases and the expression of a potent angiogenic inducer protein, VEGF were confirmed by dot-blot analysis using the TranSignal Human Angiogenesis Antibody Array. Dengue virus-infected HPMEC-ST1.6R cells exhibited an elongated cytoplasmic morphology, possibly representing a response to VEGF and activation of angiogenesis. The increased levels of Th-1 cytokines and VEGF in DENV-2 virus infected-HPMEC-ST1.6R could be distinguished from those infected by DENV-3. This suggests that cytokine patterns associated with DENV infections may be serotype and strain-specific. The experimental approaches described here could be developed further into a useful diagnostic tool for the characterization of dengue hemorrhagic fever cases, leading to enhancement of treatment therapy.
Asunto(s)
Citocinas/biosíntesis , Virus del Dengue/fisiología , Células Endoteliales/virología , Factor A de Crecimiento Endotelial Vascular/biosíntesis , Línea Celular , Efecto Citopatogénico Viral , Células Endoteliales/citología , Técnica del Anticuerpo Fluorescente , Humanos , Immunoblotting , Pulmón/citología , Pulmón/virología , Microesferas , ARN Viral/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas Virales/análisisRESUMEN
We investigated mosquito and bird involvement in West Nile virus (WNV) transmission in July 2001 in Jefferson County, FL, and Lowndes County, GA. We detected 16 WNV-infected pools from Culex quinquefasciatus, Cx. salinarius, Cx. nigripalpus, and Culiseta melanura. In Florida, 11% of 353 bird sera neutralized WNV. Antibody prevalence was greatest in northern cardinal (Cardinalis cardinalis, 75%), northern mockingbird (Mimus polyglottus, 50%), common ground-dove (Columbina passerina, 25%), common grackle (Quiscalus quiscula, 15%), domestic chicken (Gallus gallus, 16%), and house sparrow (Passer domesticus, 11%). Antibody-positive birds were detected in nine of 11 locations, among which prevalence in chickens ranged from 0% to 100%. Seropositive chickens were detected in Georgia as well. The primary transmission cycle of WNV in the southeastern United States apparently involves Culex mosquitoes and passerine birds. Chickens are frequently infected and may serve as effective sentinels in this region.
Asunto(s)
Anticuerpos Antivirales/sangre , Enfermedades de las Aves/epidemiología , Pollos , Culicidae/virología , Insectos Vectores/virología , Fiebre del Nilo Occidental/veterinaria , Animales , Enfermedades de las Aves/transmisión , Aves , Vectores de Enfermedades , Enfermedades de las Aves de Corral/epidemiología , Enfermedades de las Aves de Corral/transmisión , Vigilancia de Guardia , Estudios Seroepidemiológicos , Sudeste de Estados Unidos/epidemiología , Fiebre del Nilo Occidental/epidemiología , Fiebre del Nilo Occidental/transmisión , Virus del Nilo OccidentalRESUMEN
In October 2002, West Nile virus (WNV) was identified in farmed American alligators (Alligator mississippiensis) in Florida showing clinical signs and having microscopic lesions indicative of central nervous system disease. To perform seroepidemiologic studies, an indirect enzyme-linked immunosorbent assay (ELISA) was developed to determine exposure of captive and wild alligators to WNV. To validate the test, a group of WNV-seropositive and -seronegative alligators were identified at the affected farm using hemagglutination inhibition (HAI) and the plaque reduction neutralization test (PRNT). The indirect ELISA utilized a rabbit anti-alligator immunoglobulins polyclonal antibody as the secondary antibody, and inactivated WNV-infected Vero cells were used as the coating antigen. For all samples (n=58), the results of the ELISA were consistent with the HAI and PRNT findings. Plasma was collected from 669 free-ranging alligators from 21 sites across Florida in April and October 2003. Four samples collected in April and six in October were positive for WNV antibodies using HAI, PRNT, and the indirect ELISA. This indicated that wild alligators in Florida have been exposed to WNV. These findings can be used as a baseline for future surveys.
Asunto(s)
Caimanes y Cocodrilos/virología , Anticuerpos Antivirales/sangre , Ensayo de Inmunoadsorción Enzimática/veterinaria , Fiebre del Nilo Occidental/veterinaria , Virus del Nilo Occidental/inmunología , Animales , Reservorios de Enfermedades/veterinaria , Ensayo de Inmunoadsorción Enzimática/métodos , Ensayo de Inmunoadsorción Enzimática/normas , Femenino , Florida/epidemiología , Masculino , Estudios Seroepidemiológicos , Fiebre del Nilo Occidental/diagnóstico , Fiebre del Nilo Occidental/epidemiologíaRESUMEN
In September and October 2002, an epizootic of neurologic disease occurred at an alligator farm in Florida (USA). Three affected American alligators (Alligator mississippiensis) were euthanatized and necropsied, and results confirmed infection with West Nile virus (WNV). The most significant microscopic lesions were a moderate heterophilic to lymphoplasmacytic meningoencephalomyelitis, necrotizing hepatitis and splenitis, pancreatic necrosis, myocardial degeneration with necrosis, mild interstitial pneumonia, heterophilic necrotizing stomatitis, and glossitis. Immunohistochemistry identified WNV antigen, with the most intense staining in liver, pancreas, spleen, and brain. Virus isolation and RNA detection by reverse transcription-polymerase chain reaction confirmed WNV infection in plasma and tissue samples. Of the tissues, liver had the highest viral loads (maximum 10(8.9) plaque-forming units [PFU]/0.5 cm3), whereas brain and spinal cord had the lowest viral loads (maximum 10(6.6) PFU/0.5 cm3 each). Virus titers in plasma ranged from 10(3.6) to 10(6.5) PFU/ml, exceeding the threshold needed to infect Culex quinquefasciatus mosquitoes (10(5) PFU/ml). Thus, alligators may serve as a vertebrate amplifying host for WNV.
Asunto(s)
Caimanes y Cocodrilos/virología , Fiebre del Nilo Occidental/veterinaria , Virus del Nilo Occidental/aislamiento & purificación , Animales , Animales Domésticos/virología , Antígenos Virales/sangre , Brotes de Enfermedades/veterinaria , Reservorios de Enfermedades/veterinaria , Femenino , Florida/epidemiología , Inmunohistoquímica/veterinaria , Hígado/patología , Hígado/virología , Masculino , ARN Viral/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Estudios Seroepidemiológicos , Carga Viral/veterinaria , Fiebre del Nilo Occidental/epidemiología , Fiebre del Nilo Occidental/patología , Virus del Nilo Occidental/inmunologíaRESUMEN
After West Nile virus (WNV) was first detected in Florida in July 2001, intensive surveillance efforts over the following five months uncovered virus activity in 65 of the state's 67 counties with 1,106 wild birds, 492 horses, 194 sentinel chickens, and 12 people found infected with the virus. Thirteen of 28 mosquito isolations came from Culex mosquitoes. As seen in the northeastern United States, wild bird mortality was the most sensitive surveillance method. However, unlike the predominantly urban 1999 and 2000 epizootics, the Florida transmission foci were rural with most activity detected in the northern part of the state. All human cases were preceded by the detection of WNV in animals; however, only eight of the twelve cases were preceded by reports of WNV activity in the county of residence. West Nile virus-positive animals detected by multiple surveillance systems preceded seven of these cases by two weeks or more.
Asunto(s)
Brotes de Enfermedades , Vigilancia de Guardia , Fiebre del Nilo Occidental/epidemiología , Fiebre del Nilo Occidental/prevención & control , Virus del Nilo Occidental/aislamiento & purificación , Animales , Aves/virología , Pollos/virología , Culex/virología , Femenino , Florida/epidemiología , Caballos/virología , Humanos , Insectos Vectores , Masculino , Persona de Mediana Edad , Estaciones del Año , Fiebre del Nilo Occidental/etiologíaRESUMEN
We describe the first documented field transmission of West Nile (WN) virus by a North American mosquito. WN was first detected in northern Florida in 2001. An intensive mosquito trapping and surveillance program was conducted in this region for four nights to assess mosquito transmission of WN. Four mosquito traps, each with a single sentinel chicken, were placed at five different locations on each of four nights. A total of 11,948 mosquitoes was collected, and 14 mosquito pools were found to contain WN, giving a minimum infection rate between 1.08 and 7.54 per 1,000. Only one of the 80 sentinel chickens seroconverted to WN, demonstrating a single mosquito transmission event during the study and a mosquito transmission rate of between 0.8 and 1 per 1,000. Culex nigripalpus Theobald was responsible for WN transmission to the sentinel chicken, although both Cx. nigripalpus and Culex quinquefasciatus Say were found infected with WN. Mosquito transmission rates are reported in this study for the first time for a WN outbreak. This information is essential to determine risk of human and animal infection.
Asunto(s)
Culex/virología , Fiebre del Nilo Occidental/transmisión , Virus del Nilo Occidental/aislamiento & purificación , Animales , Pollos , Documentación , Femenino , Florida , Geografía , Humanos , Vigilancia de GuardiaRESUMEN
We examined the prevalence of antibodies to three mosquito-borne arboviruses in blue jays, Cyanocitta cristata, and Florida scrub-jays, Aphelocoma coerulescens, to identify the effects on host survival, the influence of sex and age on infection, and the temporal patterns of antibody prevalence. Blood samples from 306 blue jays and 219 Florida scrub-jays were collected at Archbold Biological Station (Lake Placid, FL) from April 1994 through December 1995. Sera were analyzed for hemagglutination-inhibition antibody to eastern equine encephalitis (EEE) and St. Louis encephalitis (SLE) viruses, and neutralizing antibodies to EEE, Highlands J (HJ), and SLE viruses. Overall, 31.4% of blue jay samples and 22.1% of scrub-jay samples had antibodies to EEE. Antibodies to HJ were detected in slightly >15% of samples in each jay species, and SLE was detected in <3% of the samples in each jay species. A single EEE virus isolation was made from the blood of an 11-d-old scrub-jay nestling. Survival of adult blue jays seropositive to EEE was significantly lower than that of seronegative birds based on resight rates, but infection did not seem to affect survival of adult or juvenile Florida scrub-jays.
Asunto(s)
Infecciones por Arbovirus/veterinaria , Arbovirus/aislamiento & purificación , Virus de la Encefalitis Equina del Este/aislamiento & purificación , Pájaros Cantores/virología , Animales , Animales Salvajes/virología , Anticuerpos Antivirales/sangre , Infecciones por Arbovirus/epidemiología , Arbovirus/clasificación , Encefalomielitis Equina Oriental/epidemiología , Encefalomielitis Equina Oriental/veterinaria , Pruebas de Inhibición de Hemaglutinación/veterinaria , Densidad de Población , Estados Unidos/epidemiologíaRESUMEN
More than 30,000 mosquitoes in 22 species or species groups were collected from the Florida Keys, Monroe County, FL, USA, in dry ice-baited light and gravid traps. Dry ice-baited traps collected more mosquitoes than did gravid traps. West Nile virus was detected in pools of Anopheles atropos Dyar & Knab, Deinocerites cancer Theobald, and Ochlerotatus taeniorhynchus (Wiedemann).
Asunto(s)
Culicidae/virología , Virus del Nilo Occidental/aislamiento & purificación , Animales , Anopheles/clasificación , Anopheles/virología , Culex/clasificación , Culex/virología , Culicidae/clasificación , Florida , Especificidad de la EspecieRESUMEN
For a variety of infectious diseases, the richness of the community of potential host species has emerged as an important factor in pathogen transmission, whereby a higher richness of host species is associated with a lowered disease risk. The proposed mechanism driving this pattern is an increased likelihood in species-rich communities that infectious individuals will encounter dead-end hosts. Mosquito-borne pathogen systems potentially are exceptions to such "dilution effects" because mosquitoes vary their rates of use of vertebrate host species as bloodmeal sources relative to host availabilities. Such preferences may violate basic assumptions underlying the hypothesis of a dilution effect in pathogen systems. Here, we describe development of a model to predict exposure risk of sentinel chickens to eastern equine encephalitis virus (EEEV) in Walton County, Florida between 2009 and 2010 using avian species richness as well as densities of individual host species potentially important to EEEV transmission as candidate predictor variables. We found the highest support for the model that included the density of northern cardinals, a highly preferred host of mosquito vectors of EEEV, as a predictor variable. The highest-ranking model also included Culiseta melanura abundance as a predictor variable. These results suggest that mosquito preferences for vertebrate hosts influence pathogen transmission.
Asunto(s)
Virus de la Encefalitis Equina del Este/fisiología , Exposición a Riesgos Ambientales/estadística & datos numéricos , Modelos Estadísticos , Passeriformes/crecimiento & desarrollo , Animales , Pollos/virología , Culicidae/fisiología , Insectos Vectores/fisiología , Densidad de Población , Riesgo , Análisis EspacialRESUMEN
Florida has the highest degree of endemicity for eastern equine encephalitis virus (EEEV) of any state in the United States and is the only state with year-round transmission of EEEV. To further understand the viral population dynamics in Florida, the genome sequence of six EEEV isolates from central Florida were determined. These data were used to identify the most polymorphic regions of the EEEV genome from viruses isolated in Florida. The sequence of these polymorphic regions was then determined for 18 additional Florida isolates collected in four geographically distinct regions over a 20-year period. Phylogenetic analyses of these data suggested a rough temporal association of the Florida isolates, but no clustering by region or by source of the isolate. Some clustering of northeastern isolates with Florida isolates was seen, providing support for the hypothesis that Florida serves as a reservoir for the periodic introduction of EEEV into the northeastern United States.
Asunto(s)
Virus de la Encefalitis Equina del Este/clasificación , Animales , Secuencia de Bases , Cartilla de ADN , Virus de la Encefalitis Equina del Este/genética , Virus de la Encefalitis Equina del Este/aislamiento & purificación , Florida , Genoma Viral , Ratones , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Serum antibodies from myriad species, particularly birds, can provide key information regarding the transmission and the expansion of the territory of emerging pathogens. Expedient antibody analysis is constrained by a lack of species-specific reagents, a deficiency potentially highlighted by the recent swine-origin influenza A virus (H1N1) outbreak. Available methodologies present difficulties that discourage thorough serologic monitoring of potential disease vectors or hosts. Rapid high-throughput procedures that combined serum amine labeling via biotinylation, contaminant removal, and microsphere-based immunoassays for antibodies to three arboviruses were developed. Agent-specific adaptations of this simple format should facilitate expanded surveillance and diagnostic capabilities regarding pathogens of human and veterinary importance.
Asunto(s)
Anticuerpos Antivirales/sangre , Anticuerpos Antivirales/aislamiento & purificación , Técnicas de Laboratorio Clínico/métodos , Virus de la Encefalitis Equina del Este/inmunología , Virus de la Encefalitis de San Luis/inmunología , Inmunoensayo/métodos , Virus del Nilo Occidental/inmunología , Animales , Humanos , MicroesferasRESUMEN
The objective of this study was to evaluate whether indicator microbes and physical-chemical parameters were correlated with pathogens within a tidally influenced Estuary. Measurements included the analysis of physical-chemical parameters (pH, salinity, temperature, and turbidity), measurements of bacterial indicators (enterococci, fecal coliform, Escherichia coli, and total coliform), viral indicators (somatic and MS2 coliphage), viral pathogens (enterovirus by culture), and protozoan pathogens (Cryptosporidium and Giardia). All pathogen results were negative with the exception of one sample which tested positive for culturable reovirus (8.5MPN/100L). Notable physical-chemical parameters for this sample included low salinity (<1ppt) and high water temperature (31 degrees C). Indicator bacteria and indicator virus levels for this sample were within average values typically measured within the study site and were low in comparison with levels observed in other freshwater environments. Overall results suggest that high levels of bacterial and viral indicators were associated with low salinity sites.